RU2259195C2 - Method for preventing and treating animal diseases with biostimulants and hormones of tissue preparation of complex action - Google Patents

Abstract

FIELD: agriculture, veterinary science.

SUBSTANCE: the present innovation deals with normalizing the flow of coupling, mating and lambing, the rate of growth for preventing and treating acute and chronic diseases of etiology in animals. One should prepare a tissue preparation due to applying cow's ovaries without yellow bodies, thyroid and parathyroid glands, thymus, pancreas and liver of cattle up to 4-yr-aged terms. The organs mentioned should be separately kept for 9-10 d in a fridge to be separately reduced thrice with a meat chopper, diluted with fresh running water at 1:2 ratio, extracted for 4 h in glassware at room temperature, thoroughly mixed every 10-15 min, treated in water bath for 1 h at 58-59 C, separately filtered through double-layered sterile gauze to keep a filtrate 4 times at 1-d-long interval in water bath at 58-59 C for 1 h. Components should be mixed so, that their ratio in preparation should be, weight%: ovarian tissue emulsion 10.3, thyroid and parathyroid emulsion 10.3, that of thymus 10.3, that of pancreas 17.2, that of liver 20.6, ACD f-2 10.3, formalin 0.4, natural bee honey 20.6. Before injection the preparation should be mixed and introduced per 1/2 dosage into dorsal lumbar muscles at 0.02-0.04 ml/kg animal body weight. The innovation provides complex biostimulation, activation of hormonal regulation of animal body.

EFFECT: higher efficiency of prophylaxis and therapy.

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Description

The present invention relates to the economy, veterinary medicine, specifically to methods of enhancing the activity of protective devices and organs of hormonal regulation of body functions with tissue preparations in the prevention and treatment of diseases.

A known method of treating diseases of humans and animals with tissue preparations containing biostimulants. Designed in 1936 by academician V.P. Filatov. It consists in the fact that a preparation prepared in advance from the tissues of an organ of a healthy organism is implanted under the skin, injected in the form of tissue preparations, used in the form of powders, ointments, etc.

According to V.P. Filatov, the method of replacing canned tissue gives a positive result in 70-75% of cases where other methods of treating the disease were useless [2, Volume 2, p. 436].

When preparing tissue emulsions according to V.P. Filatov, a fresh organ from a healthy animal is cleaned of excess tissue, placed in a refrigerator with a temperature of plus 2-4 ° C. After 6-8 days, it is crushed 2-3 times with a meat grinder, ground in a porcelain mortar, adding 2.0 ml of isotonic sodium chloride solution to 1.0 g of minced meat. The mixture is left at room temperature for 2-4 hours, kept in a water bath at a temperature of 60-80 ° C, boiled for 2-3 minutes, filtered hot through 2 layers of sterile gauze, the filtrate is poured into genicellin vials, closed with stoppers and metal run-in, autoclaved 60 minutes at a temperature of 120 ° C or boiled fractionally the first time 75, the second time in a day - 45 minutes.

The drug is stored for up to 7 months in a dark, cool place at a temperature of 2-4 ° C, injected into cattle and horses at a dose of 12.0-14.0, pigs - 5.0-8.0, sheep and goats - 2.0-4.0 ml once or twice with an interval of 6-7 days.

According to the recommendations of V.P. Filatov and other scientists, a mixture containing 1.0 g of minced minced prepared organ and 2.0 ml of isotonic sodium chloride solution is subjected to repeated heat treatments at temperatures well above the protein coagulation temperature. Thus, the vast majority of biostimulants and other protein active substances are inactivated and do not work to the best of their ability.

We have not found any messages in the patent periodical literature on other methods for preparing tissue emulsions and developing compositions of preparations containing biostimulants, hormones, and other active substances of several organs of the same system.

The purpose of the invention is to increase the effectiveness of the impact on protective devices and organs of hormonal regulation of functions by biostimulants by hormones of a tissue preparation of complex action.

This goal is achieved by the fact that several organs of one system separately stand for 9-10 days in the refrigerator at plus 2-4 ° C, separate 3 times with a meat grinder, dilute with fresh tap water in a ratio of 1: 2, extract for 4 hours in a glass dish at room temperature , thoroughly mixed every 10-15 minutes, the hour is treated in a water bath at 58-59 ° C, the mixture is separately filtered through 2 layers of material gauze, the filtrate is kept 4 times with a daily interval in a water bath at 58-59 ° C for an hour, mix components in with The ratio shown in table 1.

Table 1
Nomenclature and ratio of components in the preparation №№ Component Name Amount, g Weight.% 1. Ovarian tissue emulsion without corpus luteum of cows 1,5 10.3 2. Tissue emulsion of the thyroid and parathyroid glands of cattle up to 4 years 1,5 10.3 3. Tissue emulsion of the thymus (goiter) gland of cattle up to 4 years 1,5 10.3 4. Pancreatic tissue emulsion of 2.5 cattle up to 4 years 2,5 17,2 5. Tissue emulsion of the liver of cattle up to 4 years 3.0 20.6 6. ASD f-2 1,5 10.3 7. Formalin 0.06 0.4 8. Natural bee honey 3.0 20.6 Total 14.56 100.0

According to the simplified technology, 1.5 kg of cow ovaries, 1.5 kg of the thyroid and parathyroid glands, 1.5 kg of the thymus (goiter) gland, 2.5 kg of the pancreas, 3.0 kg of bovine liver are cleaned from excess tissue years of age are separately placed in a refrigerator with a temperature of plus 2-4 ° C, on the 8-10th day they are crushed 3 times with a meat grinder, thoroughly mixed with 20.0 l of fresh tap water (1: 2), left in a glass dish at room temperature for 4 hours, stirring the contents every 10-15 minutes, the hour is treated in a water bath at 58-59 ° C, the mixture is filtered through 2 layers of sterile gauze, the filtrate is treated 4 times after stirring every day in a water bath at a temperature of 58-59 ° C for one hour, after cooling, add 0.6 l of formalin, 1.5 l of ASD F-2 and 3.0 l of natural bee honey, with constant stirring, the drug is packaged under sterile conditions in penicillin vials of 14.56 ± 0.5 ml, in vials closed with stoppers and run-in vials, the drug is treated 3 times every other day for 60 minutes in a water bath at 58- 59 ° C, before injection it mixes to a homogeneous mixture, lead to 1/2 part of the therapeutic dose in the lumbar dorsal muscle between the last thoracic and lumbar vertebrae from the first calculation of 0.02-0.04 ml / kg of animal body weight once or twice with an interval of 7-9 days, preventing the occurrence of anaphylactic shock.

The mechanism of action of tissue biostimulants in the prevention and treatment of diseases has not been precisely and completely elucidated [2]. It is believed that during canning in a refrigerator, tissues lacking blood supply and innervation respond to oppressive conditions by forming stimulants of biological processes, conservation factors or resistance substances [Krause and Medvedev, 2]. These substances, getting into the general blood flow of the donor, stimulate the activity of protective devices, regeneration processes, neuro-trophic regulation of functions [2].

In our opinion, in the cells of organs placed in unusual temperature conditions, deprived of an influx of nutrition and oxygen, of regulating the course of physiological processes, there is a slower process of biological gradual dying and decomposition, but the decomposition is not complete, but determined by the period of stay of organs under unusual conditions, under this in their cells formed the substance of the struggle for life in unusual conditions, for the revival of lost physiological processes, the further intensity of their actions depends on the safety integral of their structures that are not inactivated by temperatures above 59 ° C, retaining their structure and functions acquired during the struggle, hormones that are not processed exert temperatures that coagulate proteins, exert their specific effect regardless of the place of parenteral ingestion, but when ingested through the mouth they break down like ordinary proteins.

Thus, biostimulants and hormones preserved from inactivation by high temperatures, injected intramuscularly to a donor in the hypersensitivity zone, act primarily on the local nerve endings, the solar plexus, and after getting into the blood, they activate the activity of protective devices everywhere, but hormones, regardless of the place of parenteral administration, have a specific effect on the course of physiological processes, as well as donor hormones.

Based on the available literature and experimental observations, we can conclude about the following mechanism of action of the ingredients of the proposed drug:

- tissue emulsion of the ovaries without corpus luteum contains biostimulants and the hormone estrone (estradiol, estriol), which will affect the enlargement of the uterus, swelling of the cells of its mucous membrane, the appearance of mucous secretions on the walls of her and the vagina, the increase in the mammary gland, the maturation of follicles, the appearance estrus and hunting, male admission and copulation with it, fertilization of the egg, normalization of the nutrition of the zygote, fetus, obtaining a healthy offspring;

- tissue emulsion of the thyroid and parathyroid glands, in addition to specific biostimulants, will contain iodine-containing hormone thyroxin, which stimulates the course of oxidative processes in tissue cells, their absorption of oxygen and carbon dioxide, and the parathyroid hormone - parathyroid hormone - will take part in the regulation of a certain ratio in the blood plasma of calcium salts and phosphorus, in violation of this ratio, seizures of central origin occur due to excitation of the motor centers of the middle and lgovatogo brain;

- tissue emulsion of the thymus (goiter) gland will affect the activity of body defenses, promote the production of antibodies, influence the development of lymphoid cells, accelerate the process of rejection of foreign transplants, and help prevent the development of malignant neoplasms;

- tissue emulsion of the pancreas contains biostimulants and hormones. They will contribute to the breakdown of proteins, fats, carbohydrates in the intestine into simple compounds that can be absorbed into the blood of the portal vein, get into the liver, where they undergo the processes of the body, the hormone insulin will affect the process of converting glucose to glycogen.

It was determined [9] that after the introduction of 1 ME of medical insulin once per decade per 1 kg of pig body weight, their weight increases, up to 5.7 kg more than in control analog animals;

- tissue emulsion of the liver containing specific biostimulants, glycogen and other substances will affect the synthesis of proteins, glycogen, fats characteristic of this animal, deamination, transamination and decarboxylation of amino acids, the conversion of ammonia to urea, and the neutralization of indole, skatol, phenol and other poisonous substances from the intestines, on the synthesis of cyancobalamin, retinol preservation;

- drug ASD f-2 - a product of the distillation of meat and bone meal, in which proteins, fats and carbohydrates are gradually broken down to low molecular weight components similar in chemical structure to cell metabolites, contains inorganic and organic nitrogen compounds, choline esters of carboxylic acids. As a result, the f-2 ASD has a high biological activity, has a neurotropic cholinomimetic effect on the central and autonomic nervous systems, stimulates the motor and secretory function of the gastrointestinal tract, improves the assimilation of nutrients by the donor organism, activates the activity of tissue enzymes, improves tissue trophism, increases the level of metabolic processes in various pathological areas, accelerates tissue regeneration in them [1, 6], has a strong antiseptic and antimi microbial action [10];

- formalin is a 40% solution of formaldehyde in water, with the addition of up to 1% methyl alcohol of formic acid, which protects formalin from polymerization.

Formalin - a preservative - has a strong antimicrobial and bactericidal effect, protects the drug from decomposition.

After injecting the drug to the donor, formaldehyde in its body in the bulk is quite quickly oxidized to formic acid, partially converted to hexamethylenetetramine and excreted in the form of these compounds by the kidneys, gastric glands, and bronchi, while acting antiseptically [7, p. 476].

Formalin is widely used in the treatment of cultures of pathogens of infectious diseases in the preparation of formol vaccine, containing from 0.1 to 0.8% formalin [2, volume 2, p.436];

- natural bee honey contains energetic material, is to some extent a prolongator of the action of the substances of the drug, a biostimulant. After the introduction, it supplies the local tissues with carbohydrates, through irritation with essential oils of the nerve endings of the tissues, the solar plexus will help normalize the functions of the body and the voluntary organ.

During storage, the drug is stratified into the upper almost transparent and lower opaque, sedimentary parts. Therefore, the drug after heating in a bottle to 35-39 ° C is shaken until a homogeneous mass.

After injection in the recipient's body, the substances of the drug that are in solution begin to act at the beginning, then the substances that are in the sediment undergo an enzymatic process. At the same time, active substances that are formed in the enzymatic process of the breakdown of sediment substances enter the body.

In the process of combating specific protein formations that have dug into the body, first local and then general protective devices are activated and enter into the struggle, the actions of enzymatic, motor, secret incretory and other processes are activated, they seem to come out of rest, “shake” from forced unregulated inactivity, the whole body, under the control of the nervous hormonal systems, enters into the struggle, and above all, with the pathological process in the diseased organ, as the most powerful irritant.

Preliminary tests of the action of biostimulants and hormones of a tissue preparation with a complex effect on protective devices and organs of hormonal regulation of functions in the body of laboratory animals gave quite encouraging results, experiments are continuing on domestic animals.

We believe that the proposed drug will find wide application in animal husbandry and veterinary medicine in normalizing the course of sexual hunting and crying, growth and development rates, in the prevention and treatment of acute and chronic diseases of non-infectious etiology.

Literature

1. Abramov S.S., Arestov I.T., Karput I.M. Prevention of non-communicable diseases of young animals. M., 1990, p.24-56

2. Veterinary Encyclopedic Dictionary. M .: Kolos. 1951, Volume 2. S.436, 500.

3. Golikov A.N., Parshutin G.V. (edited by). Physiology of farm animals. M .: Kolos, 1980, p.205-233.

4. Yoyrish N.P. Beekeeping products and their use. M .: Rosselkhozizdat. 1976, p.22-50, 86-102.

5. Degtyarev V.I., Pakin V.K., Luzyanin D.Kh. Biogenic stimulants in animal husbandry. Rostov Book Publishing House, 1960, pp. 4-9.

6. Deryabin Z.I. Determination of the biological activity of the drug ASD f-2, J. "Veterinary". 1980, p. 58-59.

7. Brains I.E. Pharmacology. M .: Selkhozdat. 1954, p. 219, 476.

8. Manual on the use of the drug ASD in veterinary medicine. Developed by the All-Russian State Research Institute of Control, Standardization and Certification of Veterinary Medicines and Armavir Biofactory. M., 1997, p. 5.

9. Obydennov V.A. Hormones in animal life. M .: Kolos, 1965, p. 24-34, 104, 120-182.

10. Savenkov K.S. Materials of the dissertation for the degree of candidate of agricultural sciences. Persianovka, DGAU, 2003

Claims (1)

A method for the prevention and treatment of animal diseases with a tissue preparation of complex biostimulating and hormonal action, prepared by preserving a healthy animal's organ in a refrigerator at plus 2-4 ° C, grinding and heat treatment, characterized in that they prepare tissue emulsions of organs, for which cows' ovaries are used without corpus luteum, thyroid and parathyroid glands, thymus, pancreas, cattle liver up to 4 years, organs separately stand 9-10 days in cold elnik, 3 times separately chopped with a meat grinder, diluted with fresh tap water in a ratio of 1: 2, extracted for 4 hours in a glass dish at room temperature, thoroughly mixed every 10-15 minutes, treated for one hour in a water bath at 58-59 ° C, separately filtered through 2 layers of sterile gauze, the filtrate was kept 4 times with a daily interval in a water bath at 58-59 ° C for an hour, then the components were mixed so that their ratio in the preparation was, wt.%: ovarian tissue emulsion - 10, 3, tissue emulsion of the thyroid and scab of the ovoid glands - 10.3, tissue emulsion of the thymus gland - 10.3, tissue emulsion of the pancreas - 17.2, tissue emulsion of the liver - 20.6, ASD f-2 - 10.3, formalin -0.4, natural honey bee - 20.6, and before injection, the drug is mixed to a homogeneous mass, 1/2 of the dose is injected into the dorsal muscles of the lower back between the last pectoral and first lumbar vertebrae at the rate of 0.02-0.04 ml / kg of animal body weight.