RU2340332C1 - Method of prevention of cow mastitis - Google Patents

Abstract

FIELD: medicine; veterinary science.

SUBSTANCE: administer a biogenic stimulator in the form of a fabric emulsion. As a biogenic stimulator use the fabric emulsion obtained from an udder, caruncles of the pregnant cow or after uterus maternity, a thymic gland, a thoracal and abdominal aorta of cattle stock to the age of 4-6 years. Thus an udder, uterus caruncles, thymic gland both the thoracal and abdominal aorta take in amount of 3; 2.5; 2.5 and 2 weight fraction accordingly. Then add ASD f-2, potassium iodide, natrium benzoicum caffeine and formalin to the tissue emulsion with such calculation so that in a ready preparation there was a following parity of components by quantity, g, and wt %: tissue emulsion - 11 g, 75.4%; ASD f-2-0.5 g, 3.4%; potassium iodide - 1.5 g, 10.3%, natrium benzoicum caffeine - 1.5 g, 10.3%, formalin - 0.09, 0.6%. Introduction of a biogenic stimulator is carried out intramusculary on 1/2 parts of a dose from calculation of mass of a body by of 0.03-0.06 ml/kg on both sides of a spine column in a crest area.

EFFECT: high efficiency and harmlessness.

Description

The present invention relates to veterinary medicine, specifically to methods for the prevention and treatment of diseases of the mammary glands of animals.

Since 1936, a method of treating diseases of people and animals with tissue preparations containing biostimulants and other substances that activate the activity of the patient’s organs (recipient), which are formed in the tissues of the body when stored in the refrigerator at + 2-4 ° C during the struggle of living cells for life, is known , which cured diseases in 70-75% of cases where other methods of treatment were useless [2, 5].

According to V.P. Filatov, when preparing a tissue preparation (emulsion), a fresh organ of a clinically and hematologically healthy animal is cleaned of excess tissue, placed in a refrigerator with a temperature of plus 2-4 ° C. After 6-8 days, it is chopped 2-3 times with a meat grinder, the ground parts are ground in a porcelain mortar, 2.0 ml of 0.9% sodium chloride solution are added to 1.0 g of minced meat. The mixture is left at room temperature for 2-4 hours, kept in a water bath for 60 hours at 60-80 ° C, boiled for 2-3 minutes, filtered hot through two layers of sterile gauze, poured into penicillin vials, they are closed with rubber stoppers and metal run-in, autoclave for 60 minutes at 120 ° C or boil fractionally for the first time for 75 minutes, for the second time in a day - 45 minutes, keep at plus 2-4 ° C in a dark dry place for up to 7 months, inject cattle subcutaneously or intramuscularly and horses at a dose of 12-14, pigs - 2-8, sheep and goats - 2-4 ml once or twice with an interval of 6-7 days.

We have not found any reports in the patent and periodic literature on the treatment of diseases of the udder with tissue preparations.

The purpose of the invention is the creation of a tissue preparation that has a beneficial effect in the prevention and treatment of acute and chronic diseases of the udder of cows.

This goal is achieved by the fact that as a biogenic stimulator use tissue emulsion obtained from the udder, caruncles of the pregnant woman or after childbirth of the uterus, thymus, chest and abdominal aorta of cattle up to 4-6 years of age, while the udder, caruncles of the uterus, the thymus, thoracic and abdominal aorta are taken in an amount of 3; 2.5; 2.5 and 2 parts by weight respectively, and then to the tissue emulsions add ASD f-2, potassium iodide, caffeine sodium benzoate and formalin so that in the finished product there was the following ratio of components in quantity, g and% by weight: tissue emulsion - 11.0 g 75.4%; ASD f-2 - 0.5 g, 3.4%; potassium iodide - 1.5 g, 10.3%; caffeine sodium benzoate - 0.5 g, 10.3%; formalin - 0.09 g, 0.6%; and the introduction of a biogenic stimulator is carried out intramuscularly in 1/2 of the dose at the rate of 0.03-0.06 ml / kg body weight on both sides of the spinal column in the withers.

Preparation technology: 30.0 g (30.0%) udders, better than lactating, 25.0 g (25.0%) of the pregnant or postpartum carunculus, cleared of excess tissue, 25.0 g (25.0%) of the thymus (goiter) gland, 20.0 g (20.0%) of the thoracic and abdominal aorta of cattle, up to 4-6 years of age, are cut with a sharp knife into pieces of the same size, put the organs separately in separate sterile glass lockable containers with a sufficient margin air and place them in a refrigerator with a temperature of plus 2-4 ° C, they can withstand the aorta for up to 15 days, parenchymal organs about 8 days.

Then the organs are jointly crushed three times by a meat grinder, preferably not a metal one, the minced meat is diluted with a double amount of 0.9% sterile sodium chloride solution and left at room temperature for four hours with stirring after 10-15 minutes, the mixture is filtered through two layers of sterile gauze. the resulting liquid 3-4 times with a daily interval is subjected to heat treatment in a water bath at a temperature of 58-59 ° C, checked for sterility.

After cooling to room temperature under sterile conditions, 0.5 g of f-2 ASD, 1.5 g of potassium iodide and caffeine sodium benzoate, 0.09 g of formalin are added to stirring on 11.0 g of a fabric emulsion with stirring; 14.0 ml in sterile penicillin vials. They are closed with rubber stoppers and metal run-ins, they are subjected to hourly heat treatments in a water bath at 58-59 ° C 2-3 times with a daily interval, they are checked for sterility, the preparation is stored in closed bottles at plus 2-4 ° C.

Pharmacodynamics:

- the udder of a cow is located in the inguinal region between the hips, built of an active glandular parenchyma and connective tissue stroma containing a certain amount of adipose tissue [1, p. 214-215].

The udder product - milk - has a complex chemical composition. It contains more than 100 different substances, including 30 fatty acids, 20 amino acids, up to 40 different mineral substances, some of its components - casein, lactose - are not contained in any other natural product [3, p.266-278].

Naturally, when the udder is in pieces for 6-8 days in the refrigerator at plus 2-4 ° C and sufficient aeration, these substances will undergo significant changes with the formation of different in strength and direction of action of biostimulants and other substances that activate the activity of the diseased organ and systems organs of a sick body;

- uterine caruncles increase significantly in size and activity during the pregnancy of the cow, are the connecting links between the mother and the fetus, through them nutrients, vitamins, protective bodies, atmospheric oxygen, hormones and much more are needed for normal life and baby development, and vice versa - carbon dioxide, metabolic products, etc.

When in unusual conditions for life and sufficient aeration, half-life products, biostimulants and other substances are formed in the tissues of the caruncles, activating the activity of the organs of the donor’s body after drug administration [3, pp. 256-267];

- The thymus (goiter) gland is highly developed in cattle up to 4-6 years of age. It distinguishes between lymphoid and reticular cells; removal of the gland in newborn animals causes impaired growth, development of the body, its death.

It was established that the hormone of the gland - thymosin - stimulates the development of lymphoid cells, accelerates the rejection of foreign bodies (transplants), data on the role of the thymus gland in preventing the development of malignant tumors in the body, it is also determined that the gland is in a certain relationship with the thyroid gland that regulates metabolism substances, especially protein [3, p.230, 214-219];

- the thoracic and abdominal aorta has the most developed middle layer, capable of stretching with systole of the ventricles of the heart and driving blood further through the vessels with its diastole. It was built from elastic and smooth muscle fibers in which extraordinary biostimulants and other substances activating the activity of the organs of the donor substance will form during the struggle of cells for life under unusual conditions [1, pp. 214-215];

- 0.9% sodium chloride solution for sodium chloride contains, in addition to sodium chloride, and many other mineral substances necessary for the life and development of the animal’s body [2, p.1, p.214-215];

- ASD f-2 is a product of distillation of meat and bone meal, while proteins, fats, carbohydrates are gradually broken down to low molecular weight compounds similar in chemical structure to cell metabolites, it contains inorganic and organic nitrogenous substances, choline esters of carboxylic acids. As a result, f-2 ASD has a high biological activity, has a neurotropic cholinomimetic effect on the central and peripheral (vegetative) nervous systems, stimulates the digestive system, improves the digestibility of nutrients, activates the activity of tissue enzymes, accelerates tissue regeneration, has a strong antiseptic and antimicrobial effect [4, p. 58-59];

- potassium iodide - it is soluble in water (1: 0.75), does not contain free iodine, does not irritate tissues and nerve endings at the injection site, does not have a bactericidal effect. After a donor enters the body, the drug changes with the formation of various iodine compounds, which are part of the thyroid hormones that regulate metabolism, especially protein, affect the milk activity and the state of the udder, activate the activity of protective devices, act bactericidal, anti-inflammatory, promote resorption and neutralization of unoxidized metabolic products [6, p.323];

- sodium caffeine-benzoate - when ingested, it normalizes the functioning of the heart, helps normalize blood circulation without increasing blood pressure, increases blood flow to the vascular glomeruli of the kidneys and the formation of urine, from which toxic and toxic metabolic products are removed from the animal’s body, normalizes the functioning of the vasomotor center and cerebral cortex [6, p.142-150];

- formalin is a 40% aqueous solution of formaldehyde in the addition of methyl alcohol and formic acid, which protects formalin from polymerization.

After getting into the animal’s body with the drug, formalin oxidizes rather quickly to formic acid, partially turns into hexamethylenetetramine and in the form of these compounds is excreted from the body by the kidneys, gastric glands, bronchi, acting antiseptically [2, p. 500; 6, p. 474].

Thus, the proposed drug with its different strength and direction of action of biostimulants and medicinal substances affects the activation of various organs and organ systems of the sick animal, interacts with its various organs, such as the thymus (goiter), thyroid glands, heart, vasomotor center, cerebral cortex, blood vessels, etc.

During experimental checks of the effectiveness of the action and the safety of the drug, the following was established:

- with an overdose of 5-6 times, the drug does not change the clinical condition of white mice with a body weight of 27.0-29, 0 g, the mass of their bodies increases by 3.0-4.0 g more than the body weight of control analog mice, the drug is not administered;

- after a single administration to the cows, the signs of acute mastitis disappeared three days earlier than in the control cows, the preparation of which was not administered, the cure of the postpartum acute endometritis in experimental cows occurred 3-6 days earlier than in animals, the preparation of which was not administered.

We believe that the proposed drug will find application in veterinary medicine in the treatment of acute and chronic mastitis, acute and chronic postpartum endometritis, normalization of the reproductive cycle of cows, and the growth and development of young animals.

Literature

1. Akayevsky A.I. Anatomy of pets. M .: Kolos, 1975, p. 214-215.

2. Veterinary Encyclopedic Dictionary. M .: Selkhozizdat, 1950-1951, v. 1, p. 366, T.2, p. 236, 430, 500.

3. Golikov A.I., Parshugin G.V. The physiology of agricultural animals. M .: Kolos, 1980, p. indicated in the text.

4. Deryabin Z.I. Determination of the biological activity of the drug DSD f-2. Veterinary Medicine, 1980, p. 58-59.

5. Degtyarev V.I., Parakin V.K., Luzyain D.Kh. Biogenic stimulants in animal husbandry. Rostov Book Publishing House, 1960, p. 4-9.

6. Brains I.E. Pharmacology. M .: Selkhozizdat, 1954, p.141-146, 323, 474.

Claims (1)

A method for treating mastitis in cows, comprising administering a biogenic stimulant in the form of a tissue emulsion, characterized in that a tissue emulsion obtained from the udder, caruncle of the pregnant woman, or after childbirth of the uterus, thymus, thoracic and abdominal aorta of cattle up to 4- is used as a biogenic stimulator 6 years of age, with the udder, caruncles of the uterus, thymus and thoracic and abdominal aorta taken in an amount of 3; 2.5; 2.5 and 2 parts by weight respectively, and then to the tissue emulsion add ASD f-2, potassium iodide, caffeine sodium benzoate and formalin so that in the finished product there was the following ratio of components in quantity: tissue emulsion - 11 g (75.4 wt.%) ; ASD f-2 - 0.5 g (3.4 wt.%); potassium iodide - 1.5 g (10.3 wt.%), caffeine sodium benzoate - 1.5 g (10.3 wt.%), formalin - 0.09 g (0.6 wt.%); and the introduction of a biogenic stimulator is carried out intramuscularly in 1/2 of the dose at the rate of 0.03-0.06 ml / kg body weight on both sides of the spinal column in the withers.