RU2316329C2 - Method for manufacturing preparation for preventing and treating diseases of viral etiology in cattle and method for treating such diseases - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: the present innovation deals with preparing basic curative solution, supplementing this solution with additives followed by sterilization, quality control and packaging. As a basic preparation it is necessary to apply ACD-2 fraction, moreover, curative solution of ACD-2 fraction preparation should be prepared upon 0.9%-sodium chloride isotonic solution. As an additive one should apply liquid form of "Vivaton" preparation. The latter should be preliminarily kept under an exhauster in open glassware for the period of about 36-48 h till complete escape of ammonia odor, moreover, ready-to-use form of the preparation mentioned contains, weight%: ACD-2 fraction 4; "Vivaton" 10; 0.9%-sodium chloride isotonic solution - the rest. The suggested method of prophylaxis and treatment of diseases of viral etiology in cattle deals with introducing them with this curative preparation. Moreover, cattle should be introduced with the above-mentioned preparation prepared according to the above-mentioned technique. The innovation is simple and enables to obtain the preparation being of wide range of action.

EFFECT: higher efficiency of therapy.

7 cl, 3 ex, 1 tbl

Description

The invention relates to agriculture, more specifically to veterinary medicine and the production of biogenic and antimicrobial drugs, for the treatment and prevention of various diseases of farm animals, and can be used both on large livestock complexes and farms, and on farm and personal farms, in complex therapy animals, in particular with infectious rhinotracheitis-infectious pustular pustular vulvovaginitis in cattle.

Known tissue biostimulant from the placenta of cows and the method of its use in animal husbandry, including microwave extraction of the placenta in a 0.9% sodium chloride solution (see RF patent No. 2178705, A61K 35/50. Publ. 27.01.2002. Bull. No. 3). With good results using this biostimulant, its disadvantage is that this drug is not mass-produced.

It is also known the use in veterinary medicine of tissue preparations with a wide spectrum of action of the ASD series (Disease Stimulant Antiseptic). ASD is mass-produced; it is a universal adaptogen. The use of ASD gives positive results in the prevention and treatment of bronchitis, gastroenteritis, dyspepsia, degenerative conditions, catarrhal pneumonia, vaginitis and endometritis in cows, trichomoniasis of animals. ASD stimulates the growth and development of calves. Moreover, the products can be used as food for people without restrictions (see ASD - myths and reality. // All-Russian Information and Advertising Bulletin ZooIndustriya. LLC. IA "Partner", St. Petersburg. 05.2004, pp. 14-15).

In veterinary medicine, the most widely used drug is ASD-2ph (see the Manual on the preparation of ASD-2ph in veterinary medicine. Approved by the Department of Veterinary of the Ministry of Agriculture of the Russian Federation on February 17, 2002 No. 13-4-03 / 0528).

Inside, the drug ASD - 2ph is prescribed to animals with drinking water according to special schemes before feeding or mixed with mixed feed for morning feeding. Treatment is carried out in courses of 5 days with an interval of 2-3 days before recovery.

Outwardly, ASD-2f is used in the form of 2-20% solutions prepared in sterile physiological saline or boiled water, alone or in combination with other agents.

Some disadvantages of the recommended methods of administering drugs of the ASD series to animals include the fact that preliminary dilution and dosing of the drug in a buffer solution (liquid) is required, which is not always convenient in production and requires additional staff time. In addition, drugs of the ASD series in the injectable form, the most effective in veterinary medicine, are not produced, which somewhat limits their effectiveness and scope in veterinary medicine.

Also known is the use of the drug from plant materials "Vivaton" in veterinary medicine. "Vivaton" - a drug consisting of infusion and herbal extract in a 25% aqueous solution of ammonia. The drug "Vivaton" has an anti-inflammatory, analgesic effect, stimulates the mechanism of protection of the mammary gland (phagocytosis, etc.) in the event of mastitis. The drug "Vivaton" is used externally. For each treatment, 50-100 cm ^{3 of} “Vivaton” is used (see the Manual for the use of “Vivaton” for the treatment of cows with mastitis. Approved by the head of the Main Veterinary Administration. Ministry of Agriculture of the RSFSR. 10.11.1990).

With its positive properties, the preparations of the Vivaton series so far have limited use in veterinary medicine due to some specific properties (the presence of ammonia in the main forms) and a somewhat slow effect on the animal organism.

The objective of the invention is to develop and create an effective and convenient for use in veterinary medicine, in a production environment, a ready-made therapeutic, universal, enhanced form of a tissue preparation with a wide spectrum of action and method of its use, allowing the well-known preparation to be used for a new purpose, including in the form of injections maximum efficiency and minimum costs for mass immunization of animals that do not require additional training for personnel and with acceptable for veterinary medicine tsitsiny period, in the preparation of which would only use domestic commercially available publicly available components, and in the manufacture would use existing equipment, apparatus and controls.

The problem is solved in that as a tissue preparation for veterinary medicine, an ASD preparation is used - fraction 2 (TU 10-19-73-89), diluted in an isotonic 0.9% sodium chloride solution and supplemented with a weathered non-ammonia form of the Vivaton TU 112- preparation 84-803-3615-001-13 with the ratio of the components in the finished form of the proposed drug, wt.%:

ASD - 2 fraction four "Vivaton" veterinary ammonia weathered 10 0.9% isotonic sodium chloride solution rest

Moreover, in dysfunctional cases of cattle and calves, the drug is administered subcutaneously to cows and calves at a dose of 0.025-0.03 ml and 0.1-0.2 ml, respectively, per 1 kg of live weight before vaccination in 24 hours, with a respiratory form of cattle IRT at a dose of 0 , 1-0.2 ml per 1 kg of live weight subcutaneously for 3-5 days once a day, with a genital form of RTI of cattle subcutaneously in cows at a dose of 0.025 ml per 1 kg of live weight and into the uterine cavity once a day for 20 -25 ml for 3-5 days, with mastitis, the drug is administered intramammary at a dose of 10.0 ml twice a day with an interval of 48 hours for 3-7 days, and for prolact ki intestinal forms RTI RNC newborn calves are inside convalescent serum with drug per vial serum (200.0 mL), adding 20.0 ml of the drug, the drug is used alone or further in combination with other agents.

Preparation of the drug is carried out by sequential mixing of the components, while Vivaton is kept (weathered) before the introduction under the hood in an open method for 36-48 hours in an open container until the smell of ammonia disappears. After sterilization control and packaging, the drug is ready for the treatment of farm animals. The drug before use does not require dilution or mixing with other drugs, sterile, effective and can be used for external, internal and injection use, both independently and in complex treatment with other drugs and agents according to the proposed scheme.

The tissue veterinary preparation based on ASD in the proposed form is conditionally called "Vidor" by the authors (short for "Vivaton" + "Roads").

After conducting numerous laboratory experiments and animal studies, temporary regulations and instructions for conducting production tests of the Vidor preparation were developed and approved.

The ratio of the main active ingredients in the ASD preparation is 2 fractions of 4% by weight and Vivaton ammonia-free weathered 10%, as well as a diluting medium in the form of 0.9% isotonic sterile sodium chloride solution, were selected practically experimentally based on an analytical review and production experience authors, as contributing to the creation of a stable non-delaminating composition, convenient for injection with a sufficient period of use and high therapeutic effect.

An unobvious effect of the new tissue preparation Vidor proposed for veterinary medicine was that, being a comprehensive universal immunomodulator ready for use, the drug not only retains the effectiveness of the two main components of ASD - 2ph and Vivaton, but also exhibits new properties (can be injected), shows high efficiency from treatment according to the proposed scheme, higher separate use of components while reducing labor costs and treatment time, and a dilution medium (0.9% sterile isotoni esky sodium chloride solution) prior to use SDA and "Vivaton" was not used.

Comprehensive production tests of the drug "Vidor" for various animal diseases were carried out at agricultural enterprises of the Sverdlovsk region in 2003-2004.

In production tests used the drug "Vidor" manufactured by LLC "TRAVNIK". Quality control of the drug was carried out in the laboratories of the NIVS of Yekaterinburg in accordance with the approved temporary technical documentation.

According to organoleptic and physico-chemical indicators, the drug must meet the requirements and norms indicated in the table.

Table Characteristic of the drug "Vidor" Name Characteristic Control methods Appearance Liquid - Color Dark straw - % content of ASD-2f four% - % content of weathered "Vivaton" 10% - % content of 0.9% sterile isotonic sodium chloride solution The rest (86%) - Sterility on MPA

To conduct production tests, the drug "Vidor" produced by experimental series. The drug is packaged in glass bottles with a capacity of 0.1-0.2 l, with rubber stoppers and rolled up aluminum caps. Each packing unit is labeled with the name, manufacturing date, expiration date, and batch number on it. Each series of the drug "Vidor" is checked for sterility on MPA with an indication in the journal.

The results of some representative production tests are presented below.

Example 1. The test drug "Vidor" was carried out in 2003-2004. on the farm to them. Sverdlov Bogdanovich district of Sverdlovsk region, unfavorable for IRT-IPV, VD-BS cattle. Treatment of calves aged 2-10 days of the experimental group (652 heads) was carried out by giving “Vidor” in the amount of 20-30 ml with simultaneous subcutaneous administration of “Vidor” at a dose of 0.1-0.2 ml per 1 kg of live weight per within 3-5 days. Two colostrum drinks were replaced with Vidor, the third calves were fed with a mixture of 50% colostrum, 50% Vidor. This mixture was evaporated 3 times a day until the digestion process normalized. A control group of calves (323 heads) was treated with general therapeutic and hepatotropic drugs. The treatment of calves of the experimental group took 5-7 days, the control group 10-14 days. Of the calves in the experimental group, 91.6% recovered, and in the control group, 62.3% recovered. For joint inflammation and viral etiology (VD-BS of cattle), Vidor was administered to calves at the age of 30 days subcutaneously at a dose of 0.1-0.2 ml per 1 kg of live weight in the morning and evening for 5-7 days. Recovery occurred on the 8-10th day of treatment. The experiment involved 121 calves. 79% of the experienced calves recovered, 15% of the calves were left with residual effects in the form of lameness, 6% of the calves were handed over to the meat processing plant for sanitary rejection. The control group consisted of 96 calves. Treated by conventional therapeutic methods. From the control group, 52% of the calf recovered on the 14-18th day of treatment, with residual effects - lameness, joint inflammation remained 36% of the calves, 12% of the calves were handed over to the meat factory with a medical waste.

Example 2. In the SEC "Shilovsky" Berezovsky district of the Sverdlovsk region in the period from 2003-2004. a clinical trial of the effectiveness of treatment with the drug "Vidor" of the genital form of IRT-IPV in cows was conducted. The drug was used in cows with the clinic endometritis, vulvovaginitis, while in animals the titers of antibodies to the IRT-IPV virus - 1: 16-1: 32 were detected, the IRT-IPV virus was isolated from the vaginal swabs at 10 TCD 50 / ML (GUSNIVS data) .

The drug was used on 220 sick cows with a clinic of a genital form of IRT-IPV cattle. The control was a group of cows with a clinic of vulvovaginitis, endometritis in the amount of 51 heads, who were treated according to standard methods.

"Vidor" was administered subcutaneously to cows at a dose of 0.025 ml per 1 kg of live weight and into the uterine cavity with a syringe catheter once a day, 20-25 ml, depending on the severity of the disease, for 3-5 days. In 97% of the cows treated with Vidor, the clinical signs of the disease disappeared within 3-5 days and completely recovered, 3% of the animals had residual effects in the form of redness and a rash on the eve of the vagina, in the control group 61% of the cows observed disappearance clinical signs of the disease, in 22% of the animals after treatment, an extension of the service period was noted, 17% of the cows were delivered to a meat factory.

As a result of using the drug "Vidor" in 97% of cows, a clear recovery was noted.

Example 3. The use of the drug "Vidor" was carried out in 2003-2004. in the SEC of the SEC "Shilovsky" of the Berezovsky district of the Sverdlovsk region, which is unfavorable for IRT-IPV cattle.

The diagnosis and selection of animals in the experimental and control groups (140 and 140 animals) were carried out by veterinary specialists of the farm, with the participation of the staff of the State Scientific and Research Institute for Strategic Research. Treatment of cows of the experimental group was carried out by administering the drug "Vidor" intramammary at a dose of 10.0 ml twice with an interval of 48 hours, for 3-7 days. Along with this, Vidor (30-50 ml) was rubbed into the skin of the affected udder lobe for 2-5 minutes twice a day after milking, for 3-4 days. Treatment of cows in the control group was carried out by veterinary specialists of the farm.

140 cows with a diagnosis of serous mastitis, catarrhal mastitis, purulent-catarrhal, subclinical mastitis, and udder edema were selected in the experimental group. As a result of treatment with Vidor, 98% of the animals recovered. In 2% of cases, the cows had a lump consolidation, where there were papillomas, during the treatment they disappeared, there were no lesions on the skin of the nipples. The average duration of treatment was 3-7 days, with severe mastitis - 3 days, catarrhal - 6 days, purulent-catarrhal - 6 days, subclinical - 3 days, with serous edema of the udder - 2 days.

The control group consisted of 140 goals, the treatment was carried out by generally accepted methods (mastisan, oxytetracycline, penicillin, streptomycin). 27% of treated cows recovered.

During treatment, it was found that the proposed drug "Vidor" and the method of its use give a high and stable therapeutic effect in various lesions of the udder of the viral etiology in cows. The effectiveness of the method is higher than the treatment methods and drugs used during the period in the household.

The method of application of the drug is simple, not laborious, does not lead to restrictions in the use of milk and can be recommended for widespread use.

Long-term versatile successful production tests in the conditions of industrial animal husbandry, accompanied by research, suggest that the use of the injection form of "Vidora" does not cause allergic reactions, side effects and complications. The drug does not have a nephro- and hepatotoxic effect with this dosage regimen.

Vidor as a complex activator of the immune system can be used for the treatment and prevention of projective activity before vaccination with cattle IRT (infectious renotracheitis in cattle).

With mastitis of herpes etiology.

With enteritis caused by cattle ИРТ.

The parenteral administration of Vidor in combination with vaccine prophylaxis in cattle IRT is an effective way to reduce clinical relapses and significantly reduce their frequency in the long term.

The appointment of "Vidor" in the course of treatment contributes to a faster re-epithelialization and a more pronounced lengthening of remission in cattle IRT.

According to the results of studies in dysfunctional cattle farms with cattle and irenitis, it was recommended for cows and calves to administer Vidor subcutaneously at a dose of 0.025-0.03 ml and 0.1-0.2 ml, respectively, per 1 kg of weight before vaccination in 24 hours.

With a respiratory form of cattle IRT, the treatment of sick calves is carried out by Vidor at a dose of 0.1-0.2 ml per 1 kg of live weight subcutaneously for 3-5 days once a day.

With the brilliant form of cattle IRT, Vidor is administered to cows subcutaneously at a dose of 0.025 ml per 1 kg of live weight and into the uterine cavity with a syringe-catheter once a day, 20-25 ml, depending on the severity of the disease, for 3-5 days .

With mastitis, the drug is administered intramammary at a dose of 10.0 ml twice a day with an interval of 48 hours for 3-7 days. In the most severe cases of the disease, it is recommended to administer the drug not only in the affected quarter, but also in the remaining four udders, as well as subcutaneously, once a day, for 3-5 days, 10.0 ml.

For the prevention of the intestinal form of cattle IRT, newborn calves are given inside serum of convalescents with Vidor - 20.0 ml of Vidor are added to a 200.0 ml serum bottle and they are evaporated at the dose indicated above.

"Vidor" with the proposed method of use expands the range of effective agents for treating farm animals, does not have a general and local adverse effect on the animal organism and livestock products and can be recommended for widespread use in veterinary medicine.

Claims (7)

1. The method of preparation of the drug for the prevention and treatment of diseases of viral etiology in cattle, including the manufacture of a basic treatment solution, the introduction of additives into the solution, sterilization, quality control and packaging, characterized in that ASD - 2 fraction is used as the basic preparation, the treatment solution of the drug ASD - 2 fractions are prepared on a 0.9% isotonic solution of sodium chloride, and as an additive, the liquid form of the drug "Vivaton" is used, which is previously held under an extract in the open the dishes for 36-48 hours until the complete disappearance of the smell of ammonia, while the finished form of the drug contains, wt.%: ASD - 2 fraction four Vivaton 10 0.9% isotonic sodium chloride solution rest 2. A method for the prevention and treatment of diseases of viral etiology in cattle, including the introduction of a therapeutic drug, characterized in that the cattle is administered a drug prepared according to claim 1. 3. The method according to claim 2, characterized in that for the prophylaxis of the intestinal form of infectious rhinotracheitis, the newborn calves are orally administered the drug, replacing colostrum in two drinks, and from the third drink, a mixture of 50% colostrum and 50% of the drug is administered, which is given 3 times a day to normalize the digestive process. 4. The method according to claim 2, characterized in that in the respiratory form of infectious rhinotracheitis, the drug is administered to cattle at a dose of 0.1-0.2 ml per 1 kg of live weight subcutaneously and 20-30 ml orally for 3-5 days once a day. 5. The method according to claim 2, characterized in that with the genital form of infectious rhinotracheitis, the drug is administered to the cows subcutaneously at a dose of 0.025 ml per 1 kg of live weight and intrauterine at a dose of 20-25 ml for 3-5 days once a day. 6. The method according to claim 2, characterized in that for mastitis of viral etiology, the drug is administered intramammally at a dose of 10 ml with an interval of 48 hours for 3-7 days, while 30-50 ml of the drug is rubbed into the skin of the affected udder for 2 -5 min twice a day after milking for 3-4 days. 7. The method according to claim 2, characterized in that for inflammation of the joints of viral etiology, the drug is administered to calves subcutaneously at a dose of 0.1-0.2 ml per 1 kg of live weight in the morning and evening for 5-7 days.