RU2345523C2 - Method of complex activation of animal organism defensors functioning - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: method implies introduction of a biogenic stimulator. A drug preparation containing (in g or wt %): tissue emulsion from bee brood drone bodies - 20.0 (20.0), natural fresh bee honey - 20.0 (20.0), 0.9% table salt solution in terms of sodium chloride - 59.7 (59.7), preserving agent formalin or phenol - 0.3 (0.3) is used as the biogenic stimulator. The drug preparation is introduced subcutaneously or intramuscularly into dorsal muscles to the left and to the right of the vertebral column, to the right and to the left of the neck, thigh or other parts of the body in the amount of 1/2 of a dose with the ratio of 0.05-0.08 ml/kg of the body weight.

EFFECT: activating growth and development of a young animal, increasing resistance of a clinically healthy and diseased animal to pathogenic factors.

Description

The present invention relates to agriculture, veterinary medicine and livestock, specifically to methods and means of activating the growth and development of a young animal, increasing the resistance of a clinically healthy and sick animal to the action of pathogenic factors.

A known method of treating diseases of humans and animals with tissue preparations containing biostimulants that are formed in the tissues of an organ during the struggle of its living cells for life (1, 3, 4). First developed and put into practice by Academician V.P. Filatov.

When preparing a tissue preparation (emulsion) according to V.P. Filatov, the fresh organ of a clinically and hematologically healthy animal is cleaned of excess tissue, placed in a refrigerator with a temperature of plus 2-4 ° C. After 6-8 days, it is chopped two or three times with a meat grinder, ground in a porcelain mortar, adding 2.0 ml of 0.9% sodium chloride solution to 1.0 g of minced meat. The mixture is left for extraction at room temperature for 2-4 hours, kept in a water bath for 60 hours at 60-80 ° C, boiled for 2-3 minutes, filtered hot through two layers of sterile gauze, poured into penicillin vials, and closed with rubber stoppers and metal run-ins, autoclave 60 minutes at 120 ° C or boil fractionally for the first time 75, the second time in a day - 45 minutes.

The drug is stored for up to 7 months in a dark, dry place at a temperature of plus 2-4 ° C, injected into cattle and horses subcutaneously or intramuscularly at a dose of 12.0-14.0, pigs - 5.0-8.0, sheep and goats 2.0-4.0 ml once or twice with an interval of 6-7 days.

We have not found any reports in the periodic and patent literature on the preparation and use of tissue emulsion from tissues and organs of bee brood drones larvae.

The purpose of the invention is the creation of a drug that has an activating and other effect on protective devices, buffer systems of the body, affecting its immune system.

This goal is achieved by the fact that subcutaneously or intramuscularly to the animal to the left and right of the spinal column in the dorsal muscles, on the right and left neck, thigh or other parts of the body, 1/2 of the dose is administered at the rate of 0.05-0.08 ml / kg of its body weight a tissue preparation containing substances in the following ratio of components, g (wt.%): tissue emulsion from the bodies of drones of bee brood - 20.0 (20.0), fresh bee honey, natural - 20.0 (20.0 ), 0.9% sodium chloride solution for sodium chloride - 59.7 (59.7), preservative (formalin, phenol) - 0.3 (0.3).

Dividing the dose into two parts and administering them in two or more places is associated with an improvement in the simultaneous action of the drug substances on both sides of the body and to prevent significant concentrations of the drug substances in the same place, which can cause irritation and cell death at the injection site.

The technology of preparation of the drug "Sitra":

- in a farm that is safe for infectious and invasive diseases, honeycombs from a beehive with a healthy drone brood are selected, they are placed in a refrigerator with a temperature of plus 2-4 ° C. After 6-8 days, the cells are opened and light gray larvae of the same size are removed from them, crushed in a mill with a sterile container;

- minced meat in a ratio of 1: 5 is diluted with 0.9% sodium chloride solution of sodium chloride, treated for 2-5 times an hour with a daily interval in a water bath at a temperature of 58-59 ° C, not coagulating protein structures;

- the mixture is hot filtered through two layers of sterile gauze, a preservative is added to the filtrate;

- the drug is packaged in compliance with the rules of asepsis and antiseptics in sterile penicillin vials, they are closed with rubber stoppers and metal run-in, 3-5 times subjected to heat treatment in a water bath at a temperature of 58-59 ° C with a daily interval, checked for sterility;

- keep the drug in a dark, dry place at a temperature of 2-4 ° C for up to a year in hermetically sealed vials.

Pharmacodynamics of the drug "Sitra":

- an emulsion from the tissues and organs of bee drone brood larvae has an active effect, after its injection in the animal’s body, biostimulants have a complex effect on various protective and buffer systems, the immune system, activate the transition from pathological to physiological regulation of functions in a sick body, in a clinically healthy improve metabolism, which is accompanied by growth and development;

- natural fresh bee honey - a biogenic stimulant of plant origin, processed by bees, is to some extent a prolongator. When a donor enters the body, it activates biostimulants, expands their scope of activity, is an energy material for an animal, its essential oils irritate local nerve endings and, through them, activate and normalize the work of protective and buffering devices (2, 4);

- 0.9% sodium chloride solution of sodium chloride has a diverse effect due to the presence of various substances. At the injection site, its mineral substances have a weak irritating effect, after absorption they take part in the mineral metabolism, maintain the osmotic balance (4),

- formalin is a 40% aqueous solution of formaldehyde with the addition of up to 1% methyl alcohol and formic acid, which protect formalin from polymerization.

After the animal enters the body, formalin oxidizes rather quickly to formic acid, partially turns into hexamethylenetetramine and is excreted in the form of these compounds by the kidneys, gastric glands, and bronchi, thereby acting antiseptically (1, 4).

After the substance of the drug is introduced into the body in a therapeutic dose, first the activity of local, then general protective devices, buffer, immune, and other body systems is activated, and metabolism is improved.

We believe that the drug will find wide application in veterinary medicine and livestock farming.

Literature

1. Veterinary Encyclopedic Dictionary. M .: Kolos, 1951, Volume 2, p. 436, 500.

2. Yorish N.P. Beekeeping products and their use. M .: Rosselkhozizdat, 1976, p.22-50, 86-102.

3. Degtyarev V.I., Parakin V.K., Luzyanin D.Kh. Biogenic stimulants in animal husbandry. Rostov Book Publishing House, 1960, pp. 4-9.

4. Brains I.E. Pharmacology. M .: Selkhozizdat, 1954, p. 219, 346-347.

Claims (1)

A method of complex activation of the protective devices of an animal’s body, including the introduction of a biogenic stimulator, characterized in that a drug containing, g or wt.%: Tissue emulsion from the bodies of drones of a bee brood is used as a biogenic stimulator - 20.0 (20.0) , fresh bee honey, natural - 20.0 (20.0), 0.9% sodium chloride sodium chloride solution - 59.7 (59.7), formalin or phenol preservative - 0.3 (0.3) which is administered subcutaneously or intramuscularly to the right and left of the spinal column in the dorsal e muscles, the right and left neck, hip or other body parts to 1/2-th part of the dose rate of 0.05-0.08 ml / kg body weight.