RU2453321C1 - Preparation for treating animals with anti-inflammatory and antibacterial actions - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to veterinary science. The preparation contains pumpkin sludge 84-86%, walnut concentrate 10-12%, the preparation Metrosept 2-3%, emulsifier T2 1-2%.

EFFECT: preparation is safe for animals, effective in treating inflammatory processes and wound surfaces, possesses prolonged action.

4 tbl, 1 ex

Description

The invention relates to veterinary medicine, in particular to drugs for the treatment of inflammatory processes and wound surfaces.

Improving wound care is a daunting task for veterinary practice. An increase in the number of complications and dissatisfaction with the results of treatment in many cases indicate the relevance of therapy for tissue damage with a violation of their integrity.

Due to the high level of inflammatory diseases, which in most cases become chronic, which leads to an increase in treatment time, the question arises of creating new effective anti-inflammatory and antibacterial drugs for external use, especially with components of natural origin that have biological activity.

The main tasks of treating wounds in the first phase of the wound healing process are the suppression of infection, the normalization of local homeostasis, the activation of necrotic tissue rejection, the adsorption of toxic separable wounds (products of microbial and tissue decay) and the relief of pain. In the treatment of wounds in the granulation phase (phase 2), reliable protection of granulation tissue from infection and stimulation of its growth is necessary, providing conditions for an optimal local reaction and metabolic processes in the tissues.

The delay of involutional processes (subinvolution) in the genitals is accompanied by the development of deep histomorphological changes that affect reproductive ability and often lead to sterility of cows. With the development of acute postpartum subinvolution, intoxication of the animal’s body occurs, the contractile function and retraction of the uterine muscle fibers are sharply disturbed. In this case, favorable conditions are created for penetration through the open channel of the cervix into the uterine cavity of potentially pathogenic and pathogenic microorganisms, their reproduction, as a result of which uterine subinvolution can be complicated by purulent-catarrhal or purulent endometritis. According to numerous authors, of the diseases of the reproductive organs of cows, the highest percentage is in acute postpartum endometritis

A known drug for the treatment of skin diseases - dermatitis in animals, which is a waste product from processing mustard oil seeds (fus) and is a complex mixture consisting of fats, proteins, carbohydrates, vitamins and an antiseptic - allyl mustard oil inherent in mustard oil seeds. The proposed drug is effective, affordable and relatively cheap. (RF patent No. 2111781, publ. 05.27.1998).

A known preparation containing ammonia, dimexide, fus from the production of oil from pumpkin seeds and a carrier type Aerosil-380 in the following ratio of components, wt.%: 30% solution of dimexide 4-5; 0.5% ammonia solution (ammonia) 2-4; carrier Aerosil-380 2-5; fuzz - the rest. (RF patent No. 2170085, publ. 07/10/2001).

A known drug for the treatment of endometritis in cows containing tilan, trichopolum, salicylic acid, ergot extract - ergotin, aerosil and fuz from the production of oil from pumpkin seeds in the following ratio of all initial components, wt.%: Tilan - 1.0-2.0 ; Trichopolum - 1.0-3.0; ergotin - 1.0-3.0; salicylic acid - 1.0-3.0; emulsifier Aerosil-380 - 2.0-3.0; pumpkin fuz - up to 100%. (RF patent No. 2181282, publ. 04/20/2002).

A known drug for the treatment of endometritis in cows containing pumpkin fuse, dimexide, ergotin and an emulsifier in the following ratio of components, wt.%: Ergotin 0.01-0.03, dimexide 30% aqueous solution 5.0-10.0, emulsifier ( Aerosil-380) 2.0-3.0, pumpkin fuz up to 100%. (RF patent No. 2178295, publ. 20.01.2002).

Known drug "metrosept", which relates to veterinary pharmacology, namely, drugs for the treatment of gynecological diseases in cows. The drug - "metrosept" includes the following components, wt.%: Dioxidine - 0.90-1.05, gelatin - 12.88-17.20, glycerin - 49.55-51.33, distilled water - the rest. The drug is used in veterinary practice for the treatment and prevention of inflammation of the uterus of cows after complicated and pathological births - with acute postpartum, catarrhal, purulent catarrhal endometritis, after manual separation of the placenta, prolapse of the uterus, cesarean section, with metritis of varying severity of the inflammatory process. (RF patent No. 1772917, publ. 03.20.1995).

A known drug for the treatment of endometritis in cows, containing milk-ripened walnuts extract on medical dimexide, mustard pumpkin fuz and Aerosil-380 emulsifier in the following ratio, wt.%: Milk-ripened walnuts extract on medical dimexide 12, 0-15.0; emulsifier Aerosil-380 2.0-3.0; fuz mustard and pumpkin up to 100.0. (RF patent No. 2295966, publ. 03/27/2007). This drug is taken as a prototype.

The task is to create a drug for the treatment of inflammatory processes and wound surfaces with natural components that have biological activity.

The technical result from the use of this drug is the possibility of treating inflammatory processes and wound surfaces with prolonged action of anti-inflammatory and antibacterial effects, with the maximum effectiveness of biologically active substances.

The technical result is achieved by the creation of a drug for the treatment of animals with anti-inflammatory and antibacterial effects, including active, biologically active and structure-forming substances, while fuse pumpkin and drug Metrosept are used as active substances, concentrated walnut extract is used as a biologically active substance, emulsifier T2 is used as a structure-forming substance in the following ratio of components, wt.% :

Pumpkin Foose - 84-86

Walnut Concentrate - 10-12

The drug Metrosept - 2-3

Emulsifier T2 - 1-2.

Active, biologically active and structure-forming substances that are part of the claimed preparation are selected in such a way that their combination in the indicated ratio provides the maximum anti-inflammatory and antibacterial effect with the maximum maximum effectiveness of biologically active substances.

Fuzz pumpkin is a waste from the production of pumpkin oil. In its composition, it has a complex set of biologically active substances: tocopherols, carotenoids, phosphatides, amino acids, polyunsaturated fatty acids, etc., whose combined effect has a positive effect on the endocrine system, activating the activity of the reproductive organs, acting as a highly active biostimulant. Foose, as the main component of the claimed preparation, provides extension (prolongation) of the validity period of the biologically active substance included in the preparation - concentrated extract of walnut milk-ripeness and the drug Metrosept. Pumpkin fuz obtained in the production of pumpkin oil has the following chemical and amino acid compositions. The data are shown in table 1 and table 2

The chemical composition of the pumpkin fuse,%

Table 1 Indicator In air-dry matter In absolutely dry matter Moisture 1.87 - Nitrogen 4.29 4.38 Protein 26.8 27.33 Fat 50,48 51.49 Cellulose 10.59 10.33 Calcium 0.24 0.24 Fasfor 0.63 0.63 Potassium 0.52 0.53 Sodium 1.40 1.43 Sugar 5.04 5.14 Starch 1,0 1,0

Amino acid composition of the pumpkin fuse, 1 mg / g

table 2 Indicator In air-dry matter Lysine 17.52 Histidine 10.15 Arginine 8.77 Aspartic acid 15.11 Threonine 6.45 Sbrin 13.52 Glutamic acid 35.6 Proline 3.76 Glycine 17.37 Alanine 10.55 Valine 15.02 Methionine 2.77 Isoleucine 8.12 Leucine 17.63 Tyrosine 11.59 Phenylalanine 16.90

In appearance, the fuz is a liniment-like mass of green-yellow in color with the smell of pumpkin seeds.

Concentrated extract of walnut milk-ripeness - one-off extract of walnut milk-ripeness. Physico-chemical composition of the obtained concentrated extract are shown in table 3.

Table 3 Name of constituent substances Mass fraction, mg Esters of ellagic and gallic acids 800,0 Flavonoids 60.0 Carotenoids 20,0 Ascorbates 800,0 Phytosols 600,0 Nautoquinone 250,0 Calcium 15.0 Magnesium 12.0 Iron 0.3 Cobalt 4.0 mcg% Wax 200,0 Sahara 1,200.0 Potassium 600,0 Phosphorus 400,0 Zinc 4.4 Iodine 0.50 Manganese 0.15 Sulfur 1,2 Vitamins: B ₁ 0.3 mg% B ₂ 1.0 mg% PP 500.0 mg% FROM 0.4 mg% Organic acids: lemon 1,2 apple 1.8 coffee 0.4 Ethanol 60,000.0 Cell water 32,800.0

From the above data in table 3 shows that the concentrated extract of walnut is a biologically active product. The presence of ellagic and gallic acids contribute to the protection of red blood cells from hemolysis and provide a membrane-stabilizing effect.

The drug Metrosept is an antibacterial drug derived from di-N-quinoxaline oxide, a wide spectrum of action. It has a bactericidal effect. It is active against gram-positive and gram-negative pathogenic and conditionally pathogenic aerobic bacteria and anaerobic microorganisms: Staphylococcus spp., Streptococcus spp., Escherichia spp., Salmonella spp., Proteus vulgaris, Pseudomonas aeruginosa, Clostridium perfingium. Dioxidine activity increases under anaerobic conditions. Dioxidine selectively inhibits DNA synthesis in a microbial cell without affecting the synthesis of RNA and protein. It causes structural changes in the cell wall and in the structure of bacterial nucleide, inhibits the activity of pathogenicity factors - extracellular bacterial nuclease, coagulase, α-toxin. Dioxidine is active against certain bacteria that are resistant to antibiotics and sulfonamides.

Emulsifier T2 provides the ability to obtain a high quality dosage form. Allows to achieve a uniform distribution of medicinal substances. It has a pronounced effect on the release rate of active substances, which leads to a prolonged absorption rate of these substances in animal tissues.

To obtain a concentrated extract, milk ripeness nuts are used, which are poured with ethyl alcohol, then the extract is infused, filtered. Ethyl alcohol is removed.

The drug is prepared as follows. To prepare 10 kg of the drug, pumpkin fuz is weighed - 8500 g, concentrated walnut extract - 1100 g, the drug Metrosept - 250 g, emulsifier T2 - 150 g.

The drug Metrosept is prepared according to known technology. To obtain it in an amount of 250 g, the following amount of components is weighed: 2.42 g of dioxidine, 37.62 g of gelatin; 126.2 g of glycerol and 83.75 g of distilled water.

When heated to 60 ° C, weighed fuz pumpkin, concentrated walnut extract and T2 emulsifier are added to the mass. The resulting mixture is placed in a mixer and mixed until a homogeneous mass. Stand for 2 hours at room temperature for better distribution of the components and mix again. Packed in glass jars.

The drug is a mass of brown-green with a specific smell. It remains stable for 18 months when stored in a dark and cool place.

A study of the effect of the claimed preparation on wound healing processes was carried out on a sheep, which had an unsterile skin and muscle incision of 804 mm ² in the right hypochondrium and the wound surface was not treated until an inflammatory reaction with signs of suppuration of the wound occurred. We treat the wound with the claimed drug 1 time per day during the entire observation, taking into account the size of the wound, which was 4 days later - 732 mm ² (56.5%); after 7 days - 368 mm ² (78.1%); after 10 days, 186 mm ² (89%). The wound surface in this case was filled with migrating leukocytes, lymphocytes. The presence of adhesions and cellular elements are usually considered as necessary conditions preceding the regeneration of blood vessels and nerve endings. The final and strong splicing occurred after 13 days.

To treat endometritis in cows, the drug is administered intrauterine at the rate of 85-100 cm ³ using a Jean syringe, which is connected to an ebonite catheter. Depending on the state of the inflammatory process, the drug is administered with an interval of 24-48 hours.

The therapeutic activity of the claimed drug was tested in an experimental group of 75 cows. After administration of the drug to this group of cows, calving took place independently, without surgical intervention. The results in comparison with the prototypes are shown in table 4.

Table 4 Belly group
ny Number of animals The effectiveness of the treatment and prevention of endometritis Impregnated goals % goals % Experienced 75 73 97.3 71 94.6 Proto-
type of 83 80 96.4 77 92.8%

The inventive preparation is harmless to animals and has the ability to treat inflammatory processes and wound surfaces with prolonged action of anti-inflammatory and antibacterial effects, with the maximum effectiveness of biologically active substances.

Claims (1)

A drug for the treatment of animals with anti-inflammatory and antibacterial effects, including active, biologically active and structure-forming substances, characterized in that pumpkin and drug Metrosept are used as active substances, concentrated walnut extract is used as a biologically active substance, and a structure-forming substance is used use emulsifier T2 in the following ratio of components, wt.%:
Foose pumpkin 84-86 Walnut concentrate 10-12 The drug Metrosept 2-3 Emulsifier T2 1-2