RU2504222C2 - Food product with preventive medical properties - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to food industry. One proposes a biologically active product containing a water concentrate of antlers of marals and/or reindeer and/or spotted deer in an amount of 5.0-10.0 wt %, a water solution of dihydroquercetin in an amount of 1.0-2.0 wt %, honey and bee farming products - balance.

EFFECT: invention allows to manufacture a product with high organoleptic indices, mitigating fatigue and improving physical stamina and biological activity of the organism.

3 cl, 6 tbl, 4 ex

Description

The invention relates to the food industry and relates to products consisting of additives of animal origin, natural antioxidant of plant origin (bioflavonoid) and beekeeping products. The food product contains an aqueous concentrate of extracts of antlers of deer, and / or reindeer and / or sika deer in an amount of 5.0-10.0 wt.%, An aqueous solution of dihydroquercetin (taxifolin) in an amount of 1.0-2.0 wt.% . and bee products - the rest.

Additionally, it may include a syrup of carbohydrates and / or glucose, and / or sucrose, and / or fructose, and / or other sugars. Moreover, as a beekeeping product, it contains natural honey and / or beekeeping products (wax, propolis, morbidity) and compositions based on honey. The product may be in an encapsulated form. This product has high actoprotective, adaptogenic properties and geroprophylactic value. The production of the product according to the invention helps to expand the range of food products with prophylactic properties.

There is a method of increasing the biological value of bee honey (application for pat. RF №2001116740 / 13 from 06.15.2001), consisting in the introduction of natural products in honey, characterized in that decoctions and infusions of medicinal or food plants prepared at the rate of 1 are introduced into honey : 100, 10 ml of infusion per 1000 g of honey, followed by drainage of moisture to the original level. The disadvantage of this method is the multi-stage process of obtaining a biologically active product.

A known method of obtaining a biologically active product, which provides the ability to use canned antlers, liver and canned by-products of deer, namely the spleen, embryos and penises with deer testes in the form of fine (MDP) or ultrafine powder (UDP). The extraction is carried out with natural honey in a water bath at a temperature of 45-50 ° C at a ratio of raw materials: extractant 1:10 or 1:20. As an option, extraction is carried out with natural honey at a temperature of 45-50 ° C and simultaneous exposure to an ultrasonic field with a field frequency of 25 kHz with a ratio of raw materials: extractant 1:10 or 1:20 (US Pat. RF No. 2345573 dated 02/10/2009) (prototype) .

The disadvantage of this method is the technological complexity of obtaining a biologically active product

A known method of producing a food product with prophylactic properties (US Pat. RF 2150853 from 06/20/2000), consisting of additives of animal origin and beekeeping products. The food product contains crushed embryonic tissue of animals, birds, fish and reptiles in an amount of 2.0-20 wt.% And bee products - the rest. The disadvantage of this method is the technological complexity of obtaining a biologically active product and due to its multicomponent nature, in addition, there may be an increased allergic reaction to some products.

The technical result of the present invention is to obtain a product that increases physical endurance, biological activity of the body, effectively relieves fatigue, increases muscle strength, increases the threshold of pain sensitivity, and also has high organoleptic properties that can be consumed by any population group, regardless of age and state of health. Also, the technical result of the solution is to obtain a product with a long shelf life.

The claimed technical result is achieved through the use of the following biologically active products:

- an aqueous concentrate of antler extracts - a complex adaptogen, materials from experimental studies indicate that the biologically active components contained in the extracts of antlers have a hypotensive and lipotropic effect, exhibit a gonadotropic effect and antimutagenic effect, have immunostimulating properties, and enhance metabolic processes, including due to activation of oxygen metabolism, act as a pronounced reparative agent, affect the function of the thyroid gland, can be considered how tetoksikant. The use of products enriched in antler products can increase the body's non-specific resistance to the effects of adverse environmental factors, make up for nutrient deficiencies, and obtain a mechanism for a safe way to regulate and support the functions of individual organs and systems of the human body, thereby improving health, reducing morbidity, and prolonging life person;

- dihydroquercetin is an active antioxidant and has capillary-protective, anti-inflammatory, hepatoprotective properties, which makes it possible to successfully use it in the production of biologically active food additives, medicines, food [1];

- honey neutralizes the taste, enriches the product with organic acids, vitamins and trace elements. In addition, honey is an invert sugar, which allows a long time to preserve easily oxidized substances and, accordingly, provides a long shelf life of the product. Glucose, contained in large quantities in honey, quickly enters the bloodstream, participates in oxidative processes and provides energy to muscles, the brain and other vital organs.

Beekeeping products (wax, propolis, bee morbidity) are biologically active nutrients of a wide spectrum of action.

The patented composition of the product accelerates the recovery process with depleting physical activity.

The process of preparing a patentable product includes the following main technological stages:

- preparation of an aqueous concentrate of antler extracts;

- preparation of an aqueous solution of dihydroquercetin;

- preparation of a solution of natural honey;

- product blending

- product filling, clogging;

- storage and transportation.

Obtaining an aqueous concentrate from a deer antler extract consists in enzymatic hydrolysis of antler flour (deer and / or reindeer and / or sika deer) in the presence of water and proteolytic enzymes (papain and / or trypsin), followed by aqueous extraction of the hydrolyzate, centrifugation and filtration. Enzymatic hydrolysis is carried out at a weight ratio of antler flour: water 1: 1.5 for 3-4 hours at a temperature of not more than 50 ° C. The hydrolyzate is extracted at a weight ratio of hydrolyzate: water of 1: 2.5-1: 5 for 3 hours in an ultrasonic extractor, and after centrifugation and filtration, the extract containing the target product is evaporated to a solids content of 40-50% in concentrate (biochemical indicators of the concentrate from the antler extract are presented in Annexes A, B, C).

Dihydroquercetin is dissolved in water at t = 40-60 ° C based on the ratio of dihydroquercetin: water 1:20. This ratio is due to the degree of solubility of the product in water.

Honey is dissolved in a water bath and / or oven at t = 30-35 ° C and filtered.

Alternately prepared products are added to honey in the following proportions: aqueous concentrate antler extract - 5.0-10.0 wt.%, Aqueous dihydroquercetin solution - 1.0-2.0 wt.%; beekeeping products (wax, propolis, bee morbidity) - 2.0-5.0 wt.%. The resulting mixture is placed in an ultrasonic extractor and extracted for 1-2 hours at a temperature of 35-40 ° C with a radiation frequency of 27-32 kHz. In the process of extraction under the influence of ultrasound with a given frequency on the extracted mass, cavitation phenomena arise in it, pressure pulses arising in cavitation bubbles cause grinding (dispersion) to a micron level of solid particles in the liquid; homogenization of the processed product.

After the extraction process is completed, the product is drained and re-filtered.

The finished product is Packed in cans of 60, 100, 200 ml or encapsulated.

Example 1

Example 1 Product type Mass fraction, kg Antler extract concentrate 0.05 Dihydroquercytin 0.01 Beekeeping products 0.02 Natural honey up to 1.0 Example 2 Product type Mass fraction, kg Antler extract concentrate 0,075 Dihydroquercytin 0.0125 Beekeeping products 0,03 Natural honey up to 1.0 Example 3 Product type Mass fraction, kg Antler extract concentrate od Dihydroquercytin 0.02 Beekeeping products 0.05 Natural honey up to 1.0 Example 4 Product type Mass fraction, kg Antler extract concentrate 0.1 Dihydroquercytin 0.02 Beekeeping products 0.10 Natural honey up to 1.0

These examples reflect only part of the possible compositions of the proposed product and are not exhaustive for this invention.

The introduced amount of an aqueous concentrate of antler extract of 5.0-10.0 wt.% Is optimal, since its introduction of less than 5 wt.% Does not provide an effect that increases physical endurance, biological activity of the body, also immunostimulating properties, and more than 10 wt.% Worsens organoleptic characteristics of a food product with prophylactic properties, due to the predominance of the smell of antlers. An aqueous solution of dihydroquercetin (taxifolin) in an amount of 1.0-2.0 wt.% Provides the daily requirement (40-60 mg.) Of a person in this substance and has an angioprotective, antioxidant, detoxification, hepatoprotective (antitoxic), radioprotective and decongestant action, and its increase of more than 2.0 wt.% is not advisable in the pharmacological direction and economically.

The resulting product is resistant to storage and does not lose its properties. The product made according to this invention was stored at room temperature for 2 years or more. The product retained all its organoleptic characteristics, and also contributed to the improvement of the physiological parameters of the recommended population when using it.

Thus, this invention allows to expand the range of food products with prophylactic properties and to create a product with high actoprotective, adaptogenic properties and geroprophylactic value.

Test

After we established the optimal dose of components for the patented product, studies were conducted to study its general toxic effect in laboratory animals.

The study of the general toxic effect was carried out in accordance with the “Methodological recommendations for the study of the general toxic effect of pharmacological agents” (EV Arzamastsev et al., 1997).

The study of the general toxic effect allows us to identify the organs and systems of the body that are most sensitive to the studied drug, the nature and degree of pathological changes in them.

The effect of the product was studied on immature rats at the age of one month of ten days.

The animals were kept in vivarium conditions corresponding to sanitary standards, with free access to water and food, and the natural background of illumination and air temperature of 21-22 ° C. Animals were taken after 10 days of quarantine.

To exclude the possibility of group toxicity, each group of animals was housed in a separate cage. In total, 10 males and 10 females of white laboratory rats weighing 58-64 g (5 animals in each group) were used in the experiment. Experimental animals received the drug 6 times a week with a feed of 0.5 g per group (0.1 g per head).

The duration of observation of animals was 30 days. Weighing twice a week and once a week blood sampling. They controlled the general condition of animals, their behavior, the intensity and nature of motor activity, coordination of movement, response to tactile, pain, sound and light stimuli, the frequency and depth of respiratory movements, the condition of the hair and skin, the position of the tail and other indicators that may indicate toxic effects of finely divided reindeer antlers. Take into account appetite and weight gain. Peripheral blood and urine were examined. The studies were carried out by generally accepted methods (A.A. Kudryavtsev, L.A. Kudryavtseva, 1974; M.L. Belenky, 1963).

After euthanasia, animals were sampled for further histological studies of internal organs (liver, kidneys, heart, spleen). Subsequently, paraffin histological sections were prepared according to the generally accepted technique with hematoxylin and eosin staining.

The results of the studies showed that during the entire observation period no deaths of rats were recorded and no pronounced signs of intoxication were detected. Animals correctly reacted to sound and light stimuli, mucous membranes were of usual color. The condition of the animals in the experimental groups was identical to the state in the control groups, except for males of the experimental group, which noted high motor activity, increased appetite and short sleep. These differences appeared on the third day after the introduction of the product supplement into the diet.

According to our observations, the maximum activity of the males of this group appeared on the 10th day after the start of the feed supplement (animals were very mobile, quickly ate the whole feed, overturned a heavy drinker several times, constantly fiddling in the cage). Subsequently, their activity decreased, but remained higher than that of a similar group of control rats.

Assessing the physiological state of the animals of the experimental and control groups, it should be noted that we did not observe changes in the cardiovascular and respiratory systems and weight gain. In rats, feces were normally formed, urine of a light color, transparent.

In hematological blood tests of experimental and control animals, no significant differences were found.

At the end of the observation, animals were killed and an atomic autopsy was performed with a description of the state of internal organs, determination of their mass and study of the morphological structure of tissues.

When examining the internal organs, no significant changes were detected.

The results of a macroscopic examination of internal organs:

- Heart - the myocardium is full-blooded, red-brown in color, the cavities are dilated;

- Liver - red-brown, encapsulated, full-blooded;

- Kidneys - dark brown, encapsulated, full-blooded;

- Spleen - cherry-colored, smooth, shiny, with sharp edges. When assessing the mass of the internal organs of rodents from the experimental and control groups, no significant differences were found, p <0.05 (Table 1).

Table 1 The effect of the test product on the mass of the internal organs of rats Group name Number of animals, goal Heart, g Spleen, g Liver, g Kidneys, g Experienced, males 5 0.92 ± 0.04 0.58 ± 0.12 9.15 ± 0.29 1.62 ± 0.12 Control, males 5 0.83 ± 0.06 0.66 ± 0.13 9.6 ± 0.38 1.78 ± 0.02 Experienced, females 5 0.92 ± 0.04 0.48 ± 0.12 8.86 ± 0.50 1.46 ± 0.13 Control, females 5 0.89 ± 0.03 0.62 ± 0.04 8.22 ± 0.30 1.58 ± 0.13

The integral indicators of chronic intoxication of animals of the experimental and control groups are approximately the same, which indicates the absence of pathological changes in organs and indicates non-toxicity of the drug (Table 2).

table 2 Integral indicators of chronic intoxication,% Group IPHI Experienced, males 7.51 Control, males 7.84 Experienced, females 7.65 Control, females 7.75

During histological examination of organs and tissues, necrobiotic and dystrophic changes were not detected, the optimal dose of components for the patented product was established.

To determine the general physical endurance and act-protective action, a generally accepted technique was used for these types of products:

"Open field" (the number of runs in 5 minutes); “Shuttle runs” (the number of runs in 5 minutes); “Ladder”, (number of runs in 5 minutes); “Swimming” (holding on the surface of the water with a load), sec; “Hot plate” (measuring the time during which the animal is quietly on a plate heated to a certain temperature, in our case, up to 50 ° C).

The experiments were carried out on mice of the CBA line of both sexes weighing 18-20 g. Previously, studies were conducted on the optimal effective dose, which amounted to 1.0 ml / 100 g. Products obtained according to the above examples were administered intragastrically in a volume of 1.0 / 100 g once 1 hour before testing, and also repeatedly within 7 days in a volume of 1.0 ml / 100 g once a day 30 minutes before a meal. For animals of the 1st and 2nd control groups, a similar volume of a 20% aqueous solution of honey and saline was respectively administered intragastrically.

The data obtained were processed by statistically generally accepted methods for a small sample with the determination of the arithmetic mean value (M) and the arithmetic mean error (m). To assess the significance of differences, Student's test was used. Differences were considered significant with a probability of 95% (P <0.05). The data obtained are given in the table.

In studies of the effect of the product on performance in the tests "open field", "swimming", "shuttle runs" and "ladder" it was found that in example No. 3 the use of a product of this concentration on the performance of experimental animals showed a significant increase in performance in the tests.

Table 3 Biological Activity of Products Product Name of tests Toxicity "Open field" (number of runs in 5 minutes) “Shuttle runs” (number of runs in 5 minutes) "Ladder" (number of runs in 5 minutes) "Swimming" (holding on the surface of the water with a load), sec. "Hot plate" Example 1 - 16.3 ± 1.9 10.1 ± 1.4 6.82 ± 0.46 33.6 ± 2.4 9.0 ± 1.9 Example 2 - 19.6 ± 1.8 12.1 ± 1.6 8.6 ± 0.9 99.2 ± 2.0 18.4 ± 3.7 Example 3 - 28.7 ± 3.0 15.7 ± 2.2 9.5 ± 1.3 120.0 ± 4.3 20.0 ± 1.9 Example 4 - 19.3 ± 1.6 13.1 ± 1.3 8.82 ± 0.6 113.6 ± 4.3 16.4 ± 3.6 Control 1 - 18.8 ± 2.4 11.1 ± 1.1 8.1 ± 1.6 97.0 ± 2.6 12.0 ± 3.9 Control 2 - 17.8 ± 2.6 9.2 ± 1.5 5.2 ± 0.9 32.7 ± 2.8 7.5 ± 4.7

Studies of the product for pain sensitivity in the hot plate test (measuring the time during which the animal is quietly on a plate heated to a certain temperature, in our case up to 50 ° C) showed that both in Example 2 and in Example 3, the resulting product lowers the threshold of pain sensitivity.

It should be noted that when comparing the product of Example No. 3 with other Examples and control, a more pronounced biological activity was established. In Example 4, a decrease in the activity of the product was noted, but this difference is insignificant.

To study the effect of the product on non-specific resistance factors in dogs, a series of experiments were carried out, the results of which are presented in Table 4.

As can be seen from the table, feeding the resulting product causes an immunostimulating effect, which is most pronounced in Example 3, the values of which increase on average by 45-50% of the norm. In Example 4, immunostimulation is somewhat less pronounced compared to Example 3. Apparently, the use of a product with a higher content of biologically active substances somewhat inhibits the immune system of the animal organism.

Literature

1. http://www.ametis.ru/production/lavitol-dihydroquercetin. Certificate of state registration 77.99.26.9.U. 2490.4.07 dated 04/12/2007

2. Arzamastsev EV, "Guide to the experimental (preclinical) study of new pharmacological substances." M., 2000.399 p.

3. Kudryavtsev A: A. Clinical hematology of animals / A.A. Kudryavtsev, L.A. Kudryavtseva. M .: Kolos, 1974.- 399 p.

4. Belenky M.L. Elements of a quantitative assessment of the pharmacological effect. - L., 1963.168 s.

Appendix A.

Amino acid composition of a concentrate from antlers of a maral NAME MOLECULE, WEIGHT GENERAL AMINO ACIDS FREE AMINO ACIDS OH PRO 131.1 87.95 6.22 ASP 133.1 42.10 3.43 Thr 119.1 21,20 4.89 SER 105.1 30.94 7.63 GLU 147.1 90.39 68.72 PRO 115.1 84.83 27.73 GLY 75.1 160.05 42.73 ALA 89.1 79.55 51.75 Cys 240.3 4.33 8.03 Val 117.1 24.39 15,20 MET 149.2 6.22 2,50 Lie 131.2 10.55 6.47 LEU 131.2 39.73 15.94 Tyr 181.2 7.28 5.04 PHE 165.2 26.44 21.13 OH-LYS 162.2 7.78 Track. Lys 146.2 35.65 13.13 His 155.2 18.62 3.79 ARG 174.2 69.95 7.94 Hylys 162.2 - 2.54 P-SER 163.1 - 1.84 TAURINE 125.2 - 4.44 ORN 132.2 - 4.89 TRG 204.2 - 6.56 Amount 847.94 415.06 Interchangeable 238.46 129.23 Irreplaceable 609.48 285.8

Appendix B.

Vitamin Composition Indicator Content, mg / 100 g Vitamin A (Retinol) 1,2 Vitamin E (alpha-tocopherol) - Vitamin B ₁ 68.1 Vitamin B ₂ 0.24 Vitamin B ₅ (calcium pantothenate) 389.9 Vitamin B ₆ 6.8 Vitamin PP 309.1

Appendix C.

The mineral composition of antler concentrate, μg / g Chemical element Content, mcg / g Aluminum (Al) 9.58 Arsenic (As) 0.11 Boron (B) 2.23 Calcium (Ca) 1734 Cadmium (Cd) 0.018 Cobalt (Co) 0.056 Chrome (Cr) 8.61 Copper (Cu) 18.03 Iron (Fe) 106.7 Mercury (Hg) 0,022 Iodine (I) 0.41 Potassium (K) 7110 Lithium (Li) 0,092 Magnesium (Mg) 267 Manganese (Mn) 0.85 Sodium (Na) 2730 Nickel (Ni) 8.88 Phosphorus (P) 1424 Lead (Pb) 3.47 Selenium (Se) 1,56 Silicon (Si) 51.84 Tin (Sn) 0,054 Strontium (Sr) 3,51 Vanadium (V) 0.22 Zinc (Zn) 9.24

Claims (3)

1. A food product with prophylactic properties, containing an aqueous concentrate of extracts of antlers of deer, and / or reindeer, and / or sika deer in an amount of 5.0-10.0 wt.%, An aqueous solution of dihydroquercetin (taxifolin) in an amount of 1, 0-2.0 wt.%, Honey and bee products - the rest. 2. The food product according to claim 1, characterized in that it contains biologically active nutrients of beekeeping as wax products: beeswax, and / or propolis, and / or a wide range of bee honey. 3. A food product according to claims 1 and 2, characterized in that it is represented by a plastic mass and / or encapsulated form.