RU2521209C1 - Water-soluble bactericidal reparative composition - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: composition includes a bactericidal substance - catapol - in amount of 2.1-2.5 wt %, zosterin in amount of 1.1-5.0 wt % and distilled water.

EFFECT: providing a composition which stimulates a reparative process in external protective tissue, having anti-inflammatory and radioprotective action.

1 tbl, 1 ex

Description

The invention relates to medicine, veterinary medicine, namely to the field of combustology, dermatology, surgery, and is used both for treatment and for the prevention of diseases and complications in humans and animals.

It is known the use of compositions as wound healing, dermatological agents containing antiseptics, tissue metabolism stimulants.

A known composition for stimulating skin repair (wound healing), containing (wt.%): Collagen of farm animals 0.2-1.5; sulfated glycosaminoglycans 0.002-0.05; water soluble cellulose derivatives 0.05-2.5; water - the rest [RU 2092156, A61K 9/08, A61K 35/32; 10/10/1997].

A significant disadvantage of the analogue is that the composition can cause allergic reactions, since it contains protein - collagen.

The closest tool to the proposed is an antiseptic agent [RU 1517173, A61K 31/14, 04/30/1997], which relates to antimicrobial compositions, contains antiseptic catapol, sodium chloride, water, taken in certain quantitative proportions. Common signs of the claimed and known means is the use in both cases of catapol as a bactericidal substance.

The disadvantages of the prototype: in the presence of antimicrobial activity, the drug has poorly expressed reparative properties, which can negatively affect the timing of wound healing and the restoration of the active life of the individual.

The technical task and the positive result of the invention is the creation of a multifunctional tool to accelerate the healing of lesions of integumentary tissues and superficial wounds at all stages of the healing process of a wound, in particular burns of various etiologies, including radiation, as the most severe skin lesions, combined lesions. The inventive composition has the following distinctive features when used: a reduced latency, an accelerated completion of the inflammatory period, early periods and a shortened period of epithelial formation, a reduced or absent period of purulent inflammation, and a lack of toxicity and allergenicity.

The problem has been solved by obtaining a composition containing the following ingredients: as a biologically active substance with reparative activity, a component related to pectins obtained from marine plants of the Zosteraceae family is zosterin (trade names: zosterin, zosterin-ultra 30, zosterin-ultra 60, isosterite); catapol - a broad-spectrum antiseptic (exhibits bactericidal activity against staphylococci, streptococci, gram-negative bacteria - Escherichia coli and Pseudomonas aeruginosa, Proteus, Klebsiella, etc.), also has antifungal and virucidal effects (inactivates Herpes simplex virus); distilled water - up to 100 in the following weight ratios, wt.%:

Zosterin 1.0-5.0 Catapol 0.1-5.0 distilled water rest

The composition of the claimed composition is confirmed by standard methods.

An example of obtaining this composition.

To 94 g of distilled water heated to 70 ° C, add 1 g of Zosterin powder and dissolve with stirring for 1.5-2 hours. After cooling to room temperature, 5 ml of a 10% solution of catapol preparation is added to the resulting solution and stirred for 1 hour until the components are completely combined.

The effectiveness of the claimed composition was evaluated in experiments performed on white male rats weighing 200-240 g in a treatment model of radiation injury of the III-A degree. For the study, healthy animals with clean skin were used, which were quarantined for 14 days. 1 day before the experiment, the hair was removed using a warm 15% sodium sulfide solution on the intended area of the skin of the animals back, if necessary, the method of depilation of the skin area of the animal was used.

Before irradiation of the skin, the animal was fixed in a horizontal position on a specially adapted jig.

Radiation skin lesions were simulated by irradiating a portion of the depilated skin of the back of rats from a source of soft x-ray radiation on a RUM-17 apparatus at a tube voltage of 180 kV, an anode current of 15 mA, a filter of 1 mm Cu + 1 mm A1, a dose rate of 52 R / min, and skin focal length 10 cm. The dose of skin irradiation was 58 Gy, the irradiation field was 1.7 × 1.7 cm ² . The body of the animals during skin irradiation was protected by lead plates 2 mm thick. All studied solutions were applied immediately after skin irradiation and continued to be applied once daily (in the first half of the day) throughout the entire healing period of a radiation ulcer until skin epithelization - until the skin peels off and the skin is restored. As shown by experimental studies, all the studied compounds were non-toxic, did not have a local irritant effect and were well tolerated by animals with long-term local use.

The visual signs of the formation of radiation damage to the skin were evaluated: the timing and severity of signs of inflammation at the site of skin irradiation - hyperemia (redness), peeling, swelling, the duration of the inflammatory period and the formation of a focus of radiation damage to the skin (ulcer, covered with a dry crust). Reparative processes (healing of local radiation injury) were evaluated by the terms of wound healing (terms of exfoliation of the crusts), the duration and intensity of wound healing. The intensity of wound healing was evaluated by the index of wound healing, determined by the generally accepted indicator - the rate of wound healing.

Comparative data on the effectiveness of the treatment of radiation injuries of the skin of animals (rats) with solutions of zosterol, catapol and their compositions are shown in the table:

Table Experience-
group
py Solution composition Signs of radiation damage to the skin (day) Signs of healing of radiation damage to the skin (day) The average healing rate is
lazy sores
(% per day) Dates of appearance
nenia Longitudinal
soreness
period Dates of formation
crusting
(ulcers) Healing time
lazy sores Continue
healing life
lazy sores one 1,0% 7.7 ± 0.7 4.8 ± 0.5 12.7 ± 02 28.3 ± 2.4 15.8 ± 2.6 6.8 ± 1.0 zosterol 2 0.5% 7.5 ± 02 4.0 ± 0.5 12.0 ± 0.4 29.0 ± 0.8 16.0 ± 1.2 6.4 ± 0.5 catapol 3 0.5% 5.8 ± 0.4 4.2 ± 0.3 10.5 ± 02 25.2 ± 1.1 15.0 ± 1.4 6.8 ± 0.6 catapol + 1.0% zosterol four 0.1% Without treatment catapol + 5.0% zosterol 5 5.0%
catapol
+ 1.0%
zosterol Without treatment 6 2.5% 6.3 ± 0.4 2.5 ± 0.2 8.3 ± 0.3 28.0 ± 2.3 20.4 ± 2.5 5.3 ± 0.7 zosterol The control 9.3 ± 0.8 4.7 ± 0.8 14.7 ± 0.8 33.0 ± 2.9 17.5 ± 2.6 6.1 ± 0.9

Thus, the positive effect of the proposed solution lies in the fact that a multifunctional composition has been created that stimulates the reparative process in the integumentary tissues of mammals, has anti-inflammatory, radioprotective, antimicrobial effects, reduces the degree of burn damage, intended for external use in the form of a cutaneous application.

Literature

1. Afinogenov G.E., Panarin E.F. Antimicrobial polymers, St. Petersburg: Hippocrates, 1993, 261 pp.

2. Goncharenko E.N., Kudryashov Yu.B. Radiation protection products of natural origin. Successes of modern biology. 1991, vol. 3, issue 2, pp. 302-316.

3. Saxonov P.P., Shashkov B.C., Sergeev P.V. Radiation Pharmacology. M .: Medicine, 1976, p. 214-217.

4. Kolenchenko EA, Sonina L.N., Khotimchenko Yu.S. Comparative evaluation of the antioxidant activity of low esterified pectin from sea grass zostera marina and in vitro antioxidant preparations. Biology of the Sea, 2005, v. 31, No. 5, pp. 380-383.

5. Zaporozhets T.S. Comparative immunological activity of polyglycans of marine origin. Antibiotics and Chemotherapy, 2001, v. 46, No. 7, p. 6-10.

5. Vasin M.V. Means of prevention and treatment of radiation injuries. 2nd ed., Add., M., 2001,312 p.

Claims (1)

A water-soluble bactericidal reparative composition comprising the bactericidal substance catapol, characterized in that it additionally contains polysaccharide zosterol in the following ratio of components, wt.%:
Zosterin 1.1-5.0 Catapol 2.1-2.5 Distilled water rest