RU2462239C1 - Method of thrombin time normalisation in newborn calves with iron-deficient anemia - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary. Method includes introduction of ferroglucinum-75 in dose 15 mg of iron per 1 kg of live weight of calf intramuscularly, one time and drinking of glicopin in dose 6.0 mg/day is the morning during 6 days, starting simultaneously with injection of ferroglucinum.

EFFECT: claimed method makes it possible to avoid vascular complications in newborn calves with iron-deficient anemia, make the herd healthier, reduce murrain, increase volume and quality of obtained meat and milk production, obtain healthy offspring from the animals.

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Description

The invention relates to biology and veterinary medicine, namely to hemostasiology.

The closest analogue of the proposed method for normalizing trombone time in newborn calves with iron deficiency anemia can be considered RU 2403025 "Method for normalizing thrombin time for anemia in newborn calves and piglets." In the prototype, as in the claimed method, ferroglukin is used, but in the form of a double administration, spaced by 2 injections with an interval of 10 days, which lengthens the correction time without individualizing the amount of iron introduced into the calf. In addition, used in the prototype in combination with an iron preparation 2 metabolically active drugs make the method "cumbersome" and difficult to perform in industrial livestock. Moreover, the claimed method is simple and easy to use in practice with achieving correction of thrombin time in newborn calves with iron deficiency anemia for 6 days against 10 days in the prototype. In addition, glycopine (N-acetylglucosaminyl-N-acetyl-muramyl-alanyl-D-isoglutamine) (Andronova T.M., Pinegin B.V., Kozlov I.G., Ustinova G.I. Use of the glycopin immunomodulator for for the prevention and treatment of animal diseases. Guidelines. Moscow, 2009. - 12 pp.) used in the claimed method, previously neither with its isolated use, nor when combined with any means, the ability to influence the duration of thrombin time in newborn calves was not found. with iron deficiency anemia to normalize a blood clot new time.

The aim of the invention is the normalization of thrombin time in newborn calves with iron deficiency anemia.

The essence of the proposed method is that to normalize thrombin time, newborn calves with iron deficiency anemia are prescribed a solution of ferroglukin-75 (1 ml) containing 75 mg of iron in 1 ml of a solution of 15 mg of iron per 1 kg of live calf weight intramuscularly once in a combination with drinking glycopine at 6.0 mg / day in the morning (diluted in a small amount (50.0-100.0 ml) of water or milk, or colostrum intended for feeding the calf) for 6 days, starting simultaneously with the injection of ferroglukin.

The method allows to normalize the thrombin time in newborn calves with iron deficiency anemia the day after treatment is completed, translating it to a level characteristic of healthy calves, which will allow newborn calves with iron deficiency anemia to significantly reduce the risk of thrombotic complications, improve the herd, improving trophic tissue and as a result, accelerate growth and in the future increase the amount of meat and dairy products obtained, minimizing mortality.

The inventive method is as follows.

For diagnosis in newborn calves with anemia of a violation of thrombin time, it is evaluated according to the following method Z.S. Barkagan, A.P. Momot. Osnrvy diagnosis of hemostatic disorders. Moscow, 1999, Newdiamed-AO Publishing House. - p. 82).

Principle of the method: determine the clotting time of plasma under the influence of thrombin, standardized for activity on the control normal plasma. Thrombin test characterizes the kinetics of the final stage of blood coagulation - the rate of conversion of fibrinogen to fibrin.

The following reagents are used: 1. Tris buffer, 0.05M (or Michaelis buffer), pH 7.3-7.4. 2. A solution of thrombin in the buffer activity of 14-16 seconds. The working solution of the reagent is prepared in a plastic or silicone tube and used on the day of the study. It is stored at room temperature.

The research material is calf citrate plasma.

To 0.2 ml of the test plasma, heated in a water bath at + 37 ° C for 1 min, add 0.2 ml of a thrombin solution (which should have a temperature not higher than + 18-25 ° C before the study). Start the stopwatch immediately and note the coagulation time.

The determination is carried out manually or on a coagulometer.

The result is expressed in seconds, comparing the clotting time in the control normal (reference) and the studied plasma. Normally, thrombin time (TV) is 14-16 seconds. With iron deficiency anemia in calves, acceleration of TV is noted.

When accelerating TV in newborn calves with iron deficiency anemia, it is necessary to prescribe a treatment according to the claimed method: ferroglukin-75 (1 ml) at the rate of 15 mg of iron per 1 kg of calf live weight inside the muscle, once and glycopine is taken out at 6.0 mg / day in the morning in for 6 days, starting simultaneously with the injection of ferroglukin.

Example 1. In a newborn calf No. 26 with iron deficiency anemia, the 2nd day of life, body weight 31 kg, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 84.2 g / l, red blood cells 4.00 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.4 μmol / l. The examination revealed an acceleration of thrombin time (12 seconds). The calf was prescribed 6.2 ml of a solution of ferroglukin-75 intramuscularly once and the glycopine was taken out at 6.0 mg / day in the morning while mixing it with 50.0 ml of colostrum for 6 days, starting simultaneously with the injection of ferroglukin.

The day after the treatment was completed, the calf showed normalization of red blood - the amount of hemoglobin in it was 116.0 g / l, erythrocytes 4.76 × 10 ¹² / l with the number of sideocytes 7.7% and the level of iron in serum 21.2 mmol / l The animal achieved complete normalization of thrombin time (15 sec), which is stably preserved in the future.

Example 2. In a newborn calf No. 29 with iron deficiency anemia, the 4th day of life, body weight 29 kg, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 82.1 g / l, erythrocytes 3.92 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 11.3 μmol / l. The examination revealed an acceleration of thrombin time (11 seconds). The calf was prescribed 5.8 ml of ferroglukin-75 solution intramuscularly once and the glycopine was taken out at 6.0 mg / day in the morning while mixing it with 100.0 ml of milk for 6 days, starting simultaneously with the injection of ferroglukin.

The day after the end of treatment, the calf returned to normal red blood - the amount of hemoglobin in it was 118.0 g / l, erythrocytes 4.65 × 10 ¹² / l, siderocytes 7.4% with a serum iron level of 20.8 μmol / l , a complete normalization of thrombin time was achieved (16 sec), stably remaining in the future.

Using the proposed method for normalizing thrombin time in biology and veterinary medicine will help to avoid thrombotic complications in newborn calves with iron deficiency anemia, improve the herd, reduce mortality and increase the volume and quality of future meat and dairy products and get healthy offspring from these animals.

The claimed method was tested on 145 newborn calves with iron deficiency anemia, which showed in 100% of cases the effectiveness of the correction of iron deficiency anemia and thrombin time.

Claims (1)

A method of normalizing thrombin time in newborn calves with iron deficiency anemia, characterized in use in combination with ferroglukin-75 at the rate of 15 mg of iron per 1 kg of calf body weight intramuscularly, once a drug glycopin, which is drunk at 6.0 mg / day in the morning for 6 days, starting at the same time as ferroglukin injection.