RU2406485C1 - Method of thrombin time correction for newborn calves and piglets with anaemia - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention refers to veterinary science. Method involves the prescription to calves and piglets of two intramuscular injections of ferroglucin 150 mg (2 ml) every 5 days, two intramuscular injections of phosprenyl in different syringes into different points in dosage 0.09 ml/kg together with the injections of ferroglucin, and the intramuscular injections of gamavit 0.02 ml/kg once a day for five days running together with the first injection of ferroglucin.

EFFECT: method allows to normalise thrombin time in newborn calves and piglets with anaemia in 4 days after the termination of treatment to be brought close to such in healthy calves and piglets.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method of normalizing trombone time in newborn calves and piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in calves and piglets for anemia (Internal non-communicable diseases of farm animals. Edited by V. M. Danilevsky. M.: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as effective stimulants of the vital activity of a growing organism in phosprenyl, a preparation based on polyprenol phosphates obtained from needles, and gamavit, a comprehensive balanced means based on the placenta, containing sodium nucleate, a set of amino acids, vitamins, and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita // Veterinary medicine. - 2006. - No. 4. - S.13-15).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn calves and piglets with anemia in order to normalize thrombin time.

The aim of the invention is to increase the correction efficiency of thrombin time in newborn calves and piglets with anemia.

The essence of the proposed method lies in the fact that to optimize thrombin time, newborn calves and piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 5 days, phosprenyl intramuscularly, twice in different syringes at different points of 0.09 ml / kg simultaneously with injections of ferroglukin and gamavit 0.02 ml / kg intramuscularly once a day, five days in a row, starting with the first injection of ferroglukin.

The method allows to normalize the thrombin time in newborn calves and piglets with anemia 4 days after completion of treatment, translating it to a level close to that for healthy newborn calves and piglets. Subject to the recommendations of the proposed method, it is possible to maintain the optimum thrombin time in the future, which will allow newborn calves and piglets to significantly reduce the risk of microthrombotic complications, improve the herd by improving trophism of their tissues and, as a result, accelerate growth, increase the amount of meat products received and reduce case.

The inventive method is as follows.

For the diagnosis of newborn calves and piglets with anemia of a violation of thrombin time, it is evaluated according to the following method.

Principle of the method: determine the clotting time of plasma under the influence of thrombin, standardized for activity on the control normal plasma. Thrombin test characterizes the kinetics of the final stage of blood coagulation - the rate of conversion of fibrinogen to fibrin.

The following reagents are used: 1. Tris buffer, 0.05 M (or Michaelis buffer), pH 7.3-7.4.

2. A solution of thrombin in a buffer with an activity of 14-16 s. The working solution of the reagent is prepared in a plastic or silicone tube and used on the day of the study. It is stored at room temperature.

The research material is citrate platelet-rich plasma.

To 0.2 ml of the test plasma, heated in a water bath at + 37 ° C for 1 min, add 0.2 ml of a thrombin solution (which should have a temperature of no higher than + 18-25 ° C before the study). Start the stopwatch immediately and note the coagulation time.

The determination is carried out manually or on a coagulometer.

The result is expressed in seconds, comparing the clotting time in the control normal (reference) and the studied plasma. Normal thrombin time (TB) is 14-16 s. With anemia in calves and piglets, an acceleration of TV is noted.

When accelerating TB in newborn calves, it is necessary to prescribe treatment according to the claimed method: ferroglukin 150 mg intramuscularly, twice with an interval of 5 days, phosprenyl intramuscularly, twice in different syringes at different points at 0.09 ml / kg simultaneously with ferroglukin injections and gamavit 0.02 ml / kg intramuscularly once a day, for five consecutive days, starting with the first injection of ferroglukin.

Example 1. In a newborn calf No. 36, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 83.0 g / l, red blood cells 4.02 × 10 ¹² / l, siderocytes 1.1% with a serum iron level of 12.2 μmol / l with an increase in the total iron binding capacity of blood serum to 68, 6 μmol / L. The examination revealed an acceleration of thrombin time (12 s). The calf was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 5 days, phosprenyl intramuscularly, twice in different syringes at different points at 0.09 ml / kg simultaneously with injections of ferroglukin and gamavit 0.02 ml / kg intramuscularly once a day, five days in a row, starting with the first injection of ferroglukin.

4 days after the end of treatment, the calf returned to normal red blood - the amount of hemoglobin in it was 120.0 g / l, erythrocytes 5.12 × 10 ¹² / l, siderocytes 9.0% with a serum iron level of 22.0 μmol / l with a decrease in the total iron-binding ability of blood serum to 35.6 μmol / L, a complete normalization of thrombin time was achieved (15 s), which stably remained in the future.

Example 2. In a newborn piglet No. 19, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 80.0 g / l, erythrocytes 3.98 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.0 μmol / l with an increase in the total iron binding capacity of blood serum to 70, 2 μmol / L. The examination revealed an acceleration of thrombin time (11 s). A piglet was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 5 days, phosprenyl intramuscularly, twice in different syringes at different points at 0.09 ml / kg simultaneously with injections of ferroglukin and gamavit 0.02 ml / kg intramuscularly once a day, five days in a row, starting with the first injection of ferroglukin.

4 days after the end of the treatment, the red blood returned to normal in the piglet — the amount of hemoglobin in it was 122.0 g / l, erythrocytes 5.21 × 10 ¹² / l, siderocytes 9.2% with a serum iron level of 22.4 μmol / l with a decrease in the total iron-binding ability of blood serum to 33.7 μmol / L, a complete normalization of thrombin time was achieved (15 s), which stably remained in the future.

Using the proposed method for optimizing thrombin time in biology and veterinary medicine will help to avoid vascular complications in newborn calves and piglets with anemia, improve the herd, reduce mortality and increase the volume and quality of meat products and get healthy offspring from these animals.

Claims (1)

A method for correcting thrombin time in newborn calves and piglets with anemia, including the use of 150 mg ferroglukin intramuscularly, twice with an interval of 5 days, fosprenyl intramuscularly, twice in different syringes at different points at 0.09 ml / kg simultaneously with injections iron preparation and gamavit 0.02 ml / kg intramuscularly once a day, five days in a row, starting with the first injection of ferroglukin.