RU2352325C1 - Method of fast endotheliocytomy normalisation in newborn piglets suffering from anemia - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: method consists that Ferroglukin is injected intramuscularly in dosage 150 mg (2 ml) twice every 10 days. It is combined with intramuscular injections of Phosprenyl three times in different syringes into different points. For the first time Phosprenyl dosed 0.09 ml/kg is combined with Ferroglukin, for the second time Phosprenyl dosed 0.07 ml/kg is injected five days after the first injection of Ferroglukin. And for the third time Phosprenyl dosed 0.05 ml/kg is combined with the second injection of Ferroglukin. Additionally Hamavit is injected intramuscularly in dosage 0.014 ml/kg once a day within ten days since the first injection of Ferroglukin.

EFFECT: method enables to avoid various vascular complications and to optimise microcirculation and tissue trophism in newborn piglets suffering from anemia, to increase weight gain, to minimise mortality and to make the stock healthier completely.

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Description

The invention relates to veterinary medicine and biology.

Analogues of the proposed method for the rapid normalization of endotheliocythemia in newborn piglets with anemia do not exist.

Analogues of the proposed method for optimizing protein C activity in newborn piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M.: "Agropromizdat", 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as effective stimulants of the vital activity of a growing organism in phosprenyl, a drug based on polyprenol phosphates obtained from needles, and gamavit, a complex balanced means based on the placenta, containing sodium nucleate, a set of amino acids, vitamins, and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita // Veterinary medicine. - 2006. - No. 4. - p.13-15).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn piglets with anemia in order to quickly normalize endotheliocythemia.

The aim of the invention is to increase the efficiency of correction of endotheliocythemia in newborn piglets with anemia.

The essence of the proposed method is that to quickly normalize endotheliocythemia, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0.09 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin, and gamavit 0.014 ml / kg intramuscularly once per day, ten it, starting with the first ferroglyukina injection.

The method allows to normalize the level of endotheliocythemia in newborn piglets with anemia, translating it to a level close to that for healthy piglets, immediately after treatment. Subject to the data in the method of recommendations, a stable normalization of endotheliocythemia in the optimal state is possible, which will allow newborns with anemia to significantly reduce the risk of thrombotic complications, improve microcirculation, optimize trophic tissue, increase weight gain, improve livestock numbers, minimize mortality and subsequently receive healthy offspring from treated piglets.

The inventive method is as follows.

For diagnosis in newborn piglets with anemia of an increase in the level of endotheliocythemia, it is evaluated according to the following method (Zaynulina M.S. Determination of endotheliocytes in the blood. In the book: Hemostasis. Physiological mechanisms, principles for the diagnosis of the main forms of hemorrhagic diseases. St. Petersburg, 1999. - С .72-74).

The principle of the method is based on the isolation of endothelial cells together with platelets, followed by their precipitation by ADP. Venous blood in volumes of 4-5 ml is collected in test tubes. As a stabilizer, a 3.8% sodium citrate solution is used at a ratio of 1: 9. Platelet plasma is obtained by centrifugation (1000 rpm, 10 min). Platelets are separated by adding ADP (at the rate of 0.4 ml of a solution of 1 mg / ml ADP per 1 ml of supernatate), mixing the mixture for 10 min, followed by centrifugation (1000 rpm, 10-15 min). Platelet-free plasma is carefully separated from the deposited platelets and re-centrifuged (1000 rpm, 20 min). The resulting supernatant is drained and 0.1 ml of 0.9% NaCl is added to the precipitate, the precipitate is carefully separated with a glass rod from the walls and bottom of the tube. Counting the number of endothelial cells is carried out in two grids of the Goryaev chamber. The normal values of endotheliocythemia are 1-2 cells / μl. The increase in the number of circulating edothelial cells in the blood, noted in newborn piglets with anemia, reflects the degree of vascular damage and allows us to judge the severity of the disease and evaluate the effectiveness of the therapy.

With an increase in endotheliocythemia, newborn piglets with anemia are prescribed treatment according to the proposed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, the first time 0.09 ml / kg simultaneously with iron preparation, a second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin, and gamavit 0.014 ml / kg intramuscularly once a day, ten days starting from the first and ferroglukin injections.

Example. In a newborn piglet with anemia No. 11, examination revealed a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 81.0 g / l, erythrocytes 3.92 × 10 ¹² / l, siderocytes 1.2% with a level of iron in serum 12.0 μmol / l with an increase in the total iron binding capacity of blood serum to 68, 2 μmol / l, increased activity of endotheliocythemia was detected - 14 cells / μl.

The animal was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, the first time 0.09 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.05 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.014 ml / kg intramuscularly once a day, ten days starting from the first injection of ferroglukin.

The piglet was examined during the course of treatment and after its completion. Evaluation of endotheliocythemia at the end of treatment showed its normalization - 2 cells / μl.

Subsequently, the piglet showed no deviations from normal values.

Using the proposed method of normalizing endotheliocythemia in biology and veterinary medicine will help to avoid many vascular complications and optimize microcirculation and tissue trophism in newborn piglets with anemia, increase weight gain, minimize mortality and completely improve the herd.

Claims (1)

A method for the rapid normalization of endotheliocytemia in newborn piglets with anemia, characterized in that they administer 150 mg (2 ml) of ferroglukin intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, the first time 0.09 ml / kg at the same time as ferroglukin, a second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg at the same time as a second injection of ferroglukin, and hamavit is additionally administered at a dose of 0.014 ml / kg intramuscularly once a day for ten days starting with the first injection of ferroglukin.