RU2402900C1 - Method of correction of hyperfibrinogenemia at anemia of newborn calves and piglets - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to the field of veterinary medicine. The method includes the use of ferroglucine 150 mg (2 ml) intramuscularly two times with an interval of 10 days, fosprenil intramuscularly, twice in different syringes at different points, the first time 0.07 ml/kg simultaneously with the preparation of iron, the second time in a dose of 0.07 ml/kg six days after the first injection of ferroglucine and cresacine 4 mg/kg when included into the scheme of feeding for 10 days.

EFFECT: method enables to avoid many of the vascular complications of newborn calves and piglets with anemia, to restore the health of herd, to reduce mortality and increase weight gain, improve the quality and quantity of meat products, to obtain healthy offspring.

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Description

The invention relates to biology and veterinary medicine. Analogues of the proposed method for the correction of hyperfibrinogenemia in newborn calves and piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in calves and piglets in case of anemia (Internal non-communicable diseases of farm animals. Edited by V. M. Danilevsky. M.: Agropromizdat, 1991, 576 pp.).

In modern veterinary medicine, phosprenyl is used as an effective stimulant of the vital activity of a growing organism. Phosprenyl is a drug based on polyprenol phosphates obtained from needles (Deeva A.V., Salakhova Z.A., Lobova T.P. et al. Improving the safety and productivity of piglets when used phosprenyl and gamavit. // Veterinary medicine. - 2006, - No. 4, - p.13-15), and crezacin is a derivative of aroxyalkylcarboxylic acids, which shows an effective stimulating effect on living organisms, increasing the survival of animals in adverse and extreme conditions Barrier-stimulating or normalizing life processes (Baymatov VN Latypov MM Influence krezatcina on the state bird. Veterinary 2006, №11, s.49-51).

However, never before has a treatment complex consisting of ferroglukin, phosprenyl and crezacin been used in newborn calves and piglets with anemia in order to normalize hyperfibrinogenemia 3 days after the end of treatment.

The aim of the invention is to improve the correction of hyperfibrinogenemia in newborn calves and piglets with anemia.

The essence of the proposed method is that for the correction of hyperfibrinogenemia, newborn calves and piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day rehydration schedule.

The method allows to correct hyperfibrinogenemia in newborn calves and piglets with anemia 3 days after the end of treatment, translating fibrinogenemia to a level close to that for healthy calves and piglets. Subject to the recommendations of the proposed method, it is possible to maintain a subsequent level of fibrinogenemia in optimal condition, which will allow newborn calves and piglets with anemia to significantly reduce the risk of thrombotic complications.

The inventive method is as follows. Hyperfibrinogenemia is established in the examined calf and piglet by the following method (Barkagan Z.S., Momot A.P. Basics of the diagnosis of hemostasis disorders. M, 1999. - p. 84-86).

The following reagents are used: 1. Phosphate buffer, pH 7.0 (0.06 M) of the following composition: 190 ml 0.5 M KH ₂ PO ₄ (68.045 g per 1 l) and 110 ml 0.5 N sodium hydroxide. For operation, 20 ml of this basic buffer solution is brought to a volume of 100 ml with distilled water. 2. 0.15 M sodium chloride solution. 3. 0.04 M solution of monoiodoacetic acid in 0.06 M phosphate buffer (40 mg of monoiodoacetic acid in 5 ml of buffer). 4. Thrombin of a pig or a bull, respectively, 50 NIH units / ml. 5. 1.5% solution of acetic acid.

Equipment is used - a spectrophotometer with optical density measurement in the ultraviolet zone.

The material for the study is a citrate platelet-poor plasma.

1.6 ml of 0.06 M phosphate buffer was added to a test tube with 0.2 ml of plasma and placed in a water bath (+ 37 ° C). After a few minutes, 0.1 ml of a 0.04 M solution of monoiodoacetic acid was added to the mixture and after 3 minutes 0.1 ml of a thrombin solution was added. After adding each component, the contents of the tube are thoroughly mixed with a glass rod (with a rough surface at the end), leaving it in the tube. The resulting mixture is incubated at + 37 ° C for at least 15 minutes (longer incubation is allowed). The resulting fibrin clot is removed by winding onto a stick, and squeezed against the wall of the tube. The clot is washed twice in 50 ml of a 0.15 M sodium chloride solution. Then washed once in chilled distilled water. Water from the surface of the clot is additionally removed with filter paper. The resulting clot is dissolved with stirring in 5 ml of 1.5% acetic acid at room temperature. Dissolution complete in 5 minutes. The protein content in the solution is determined spectrophotometrically by measuring the absorbance at 280 and 320 nm. The extinction is stable, the measurement time can be arbitrarily selected.

The concentration of fibrinogen (X) in the test plasma sample is calculated by the formula

,

,

where E (280-320) is the difference between the values of extinction at 280 and 320 nm, thereby achieving the necessary correction for turbidity; 255 — coefficient for expressing the amount of fibrinogen in g / l in an analysis performed with 0.2 ml of plasma; 15,067 is the extinction coefficient for fibrinogen in an acidic medium at a wavelength of 280 nm (E 1% 1 cm).

Normally, the fibrinogen content determined by this method is 2.0-4.0 g / l.

When newborn piglets with hyperfibrinogenemia anemia are established, they are prescribed therapy according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day watering schedule.

Example 1. In a newborn piglet with anemia No. 17, the amount of hemoglobin was 79.0 g / l, erythrocytes 3.96 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.0 μmol / l with an increase in the total iron binding capacity blood serum up to 71.5 μmol / l, hyperfibrinogenemia level (9 g / l).

The pig was prescribed treatment according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day rehydration schedule.

3 days after the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 126.0 g / l, erythrocytes - to 4.99 × 10 ¹² / l, siderocytes to 7.7% with an increase in serum iron to 24.2 μmol / l and a decrease in the total iron binding capacity blood serum up to 34.0 μmol / l. 3 days after the end of treatment, a complete normalization of the plasma fibrinogen level (2 g / l) was achieved, which did not change in the next 2 months of observation.

Example 2. In a newborn calf with anemia No. 44, the amount of hemoglobin was 75.0 g / l, erythrocytes 3.90 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 11.0 μmol / l with an increase in total iron binding the ability of blood serum to 70.0 μmol / l, the level of hyperfibrinogenemia (8 g / l).

The calf was prescribed treatment according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day rehydration schedule.

3 days after the end of treatment, the calf returned to normal red blood. So, the amount of hemoglobin in it increased to 126.0 g / l, erythrocytes - to 5.02 × 10 ¹² / l, siderocytes to 7.9% with an increase in serum iron to 24.6 μmol / l and a decrease in the total iron binding capacity blood serum up to 32.0 μmol / l. 3 days after the end of treatment, a complete normalization of the plasma fibrinogen level (2 g / l) was achieved, which did not change in the next 2 months of observation.

Using the proposed method for the correction of hyperfibrinogenemia in biology and veterinary medicine will help to avoid many vascular complications in newborn calves and piglets with anemia, improve the herd, reduce mortality, increase weight gain, increase the quality and volume of meat products and subsequently receive healthy offspring from these animals.

Claims (1)

Method for the correction of hyperfibrinogenemia in newborn calves and piglets with anemia by applying ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the drug iron, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the drinking schedule for 10 days.