RU2383334C1 - Method for normalisation of fibrin content in blood in neonatal piglets with anaemia - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: method includes prescription of the following preparations to piglets - ferroglucinum in dose of 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polyson in dose of 4 mg/kg and krezacin in dose of 4 mg/kg with their inclusion into program of bottle-feeding for 10 days.

EFFECT: method makes it possible to correct hyperfibrinogenemia in neonatal piglets with anaemia, to further maintain level of fibrinogenemia in optimal condition, to reduce risk of thrombotic complications.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for the correction of hyperfibrinogenemia in newborn piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M.: Agropromizdat, 1991 - 576 s).

In modern veterinary medicine, polyisone, a phosphoric acid salt of 2-amino-4-methylthio- (S-oxo-S-amino) -butyric acid, is poorly soluble in water from white to cream-colored powder (Ovchinnikov) as effective stimulants of the vital activity of a growing organism in modern veterinary medicine. A. et al. Polizon - a growth stimulator // Poultry farming, 2006 - No. 12 - p.14-15), and crezacin - a derivative of aroxyalkylcarboxylic acids. It is a white crystalline powder, readily soluble in water, with a faint specific smell, sweetish-bitter taste. It exhibits an effective stimulating effect on living organisms, increasing the survival of humans and animals in adverse and extreme conditions, stimulating or normalizing life processes (Baymatov V.N., Latypov M.M.The effect of krezacin on the state of birds. Veterinary Medicine, 2006, No. 11, p. 49-51).

However, never before has a treatment complex consisting of ferroglukin, polysone and crezacin been used in newborn piglets with anemia in order to normalize hyperfibrinogenemia 3 days after the end of treatment.

The aim of the invention is to improve the correction of hyperfibrinogenemia in newborn piglets with anemia.

The essence of the proposed method lies in the fact that for the correction of hyperfibrinogenemia, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polizon 4 mg / kg and crezacin 4 mg / kg when it is included in the vaping scheme for 10 days.

The method allows to correct hyperfibrinogenemia in newborn piglets with anemia 3 days after the end of treatment, translating fibrinogenemia to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to maintain a subsequent level of fibrinogenemia in optimal condition, which will allow newborn piglets with anemia to significantly reduce the risk of thrombotic complications.

The inventive method is as follows.

Hyperfibrinogenemia is established in the examined piglet by the following method (Barkagan Z.S., Momot A.P. Basics for the diagnosis of hemostasis disorders. M., 1999 - p. 84-86).

The following reagents are used: 1. Phosphate buffer, pH 7.0 (0.06 M) of the following composition: 190 ml 0.5 M KH ₂ PO ₄ (68.045 g per 1 l) and 110 ml 0.5 N sodium hydroxide. For operation, 20 ml of this basic buffer solution is brought to a volume of 100 ml with distilled water. 2. 0.15 M sodium chloride solution. 3. 0.04 M solution of monoiodoacetic acid in 0.06 M phosphate buffer (40 mg of monoiodoacetic acid in 5 ml of buffer). 4. Pig thrombin, 50 NIH units / ml. 5. 1.5% solution of acetic acid.

Applied equipment. Spectrophotometer with optical density measurement in the ultraviolet zone.

The material for the study is a citrate platelet-poor plasma.

1.6 ml of 0.06 M phosphate buffer was added to a test tube with 0.2 ml of plasma and placed in a water bath (+ 37 ° C). After a few minutes, 0.1 ml of a 0.04 M solution of monoiodoacetic acid was added to the mixture, and after 3 minutes, 0.1 ml of a thrombin solution was added. After adding each component, the contents of the tube are thoroughly mixed with a glass rod (with a rough surface at the end), leaving it in the tube. The resulting mixture is incubated at + 37 ° C for at least 15 minutes (longer incubation is allowed). The resulting fibrin clot is removed by winding onto a stick, and squeezed against the wall of the tube. The clot is washed twice in 50 ml of a 0.15 M sodium chloride solution. Then washed once in chilled distilled water. Water from the surface of the clot is additionally removed with filter paper. The resulting clot is dissolved with stirring in 5 ml of 1.5% acetic acid at room temperature. Dissolution complete in 5 minutes. The protein content in the solution is determined spectrophotometrically by measuring the absorbance at 280 and 320 nm. The extinction is stable, the measurement time can be arbitrarily selected.

The concentration of fibrinogen (X) in the test plasma sample is calculated by the formula:

,

,

where E (280-320) is the difference between the extinction values at 280 and 320 nm, thereby achieving the necessary correction for turbidity; 255 — coefficient for expressing the amount of fibrinogen in g / l in an analysis performed with 0.2 ml of plasma; 15,067 is the extinction coefficient for fibrinogen in an acidic medium at a wavelength of 280 nm (E 1% 1 cm).

Normally, the fibrinogen content determined by this method is 2.0-4.0 g / l.

When pigs with anemia of hyperfibrinogenemia are found in newborns, they are prescribed therapy according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg when they are included in the 10-ml watering schedule days.

Example. In a newborn piglet with anemia No. 15, the amount of hemoglobin was 81.2 g / l, erythrocytes 4.01 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.8 μmol / l with an increase in the total iron binding capacity of blood serum up to 67.0 μmol / l, hyperfibrinogenemia level (8 g / l).

The piglet was prescribed treatment according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg when included in the 10-day vaping schedule. The sick pig was under dynamic observation during the entire treatment.

3 days after the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 115.0 g / l, erythrocytes - up to 4.96 × 10 ¹² / l, siderocytes to 7.1% with an increase in serum iron to 19.7 μmol / l and a decrease in the total iron binding capacity blood serum up to 38.0 μmol / l. 3 days after the end of treatment, complete normalization of plasma fibrinogen level (2 g / l) was achieved, which did not change in the next 2 months of observation.

Using the proposed method for the correction of hyperfibrinogenemia in biology and veterinary medicine will help to avoid many vascular complications in newborn piglets with anemia, improve the herd, reduce mortality, increase weight gain, increase the quality and volume of meat products and subsequently receive healthy offspring from these piglets.

Claims (1)

A method of normalizing the blood content of fibrin in newborn piglets with anemia by using ferroglukin 150 mg (2 ml) intramuscularly, twice, with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg when they are included in the 10-day watering schedule .