RU2377977C1 - Method of fibrinogenemia intensity normalisation with anemic newborn piglets - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: under the method proposed anemic piglets receive injections of ferroglucine (intramuscularly) in an amount of 75 mg (1 ml) three times at an interval of 5 days, polyzone in an amount of 3 mg per kg of body weight and crezacine in an amount of 3 mg per kg of body weigh in case of its inclusion in the feeding scheme for 12 days.

EFFECT: proposed method enables optimisation of fibrinogenemia with newborn piglets, further maintenance of fibrinogenemia level in an optimum state and reduced risk of thrombotic complications.

1 ex

Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for the correction of hyperfibrinogenemia in newborn piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, polyisone, a phosphoric acid salt of 2-amino-4-methylthio- (8-oxo-8-amino) -butyric acid, is a poorly soluble white to cream-colored powder in water (Ovchinnikov A) as effective stimulants of the vital activity of a growing organism in modern veterinary medicine. . and others. Polizon is a growth stimulator. // Poultry farming, 2006. - No. 12. - p.14-15.), and crezacin is a derivative of aroxyalkylcarboxylic acids. It is a white crystalline powder, readily soluble in water, with a faint specific smell, sweetish-bitter taste. It exhibits an effective stimulating effect on living organisms, increasing the survival of humans and animals in adverse and extreme conditions, stimulating or normalizing life processes (Baymatov V.N., Latypov M.M.The effect of krezacin on the state of birds. Veterinary Medicine, 2006, No. 11, p. 49-51).

However, never before has a treatment complex consisting of ferroglukin, polysone and krezacin been used in newborn piglets with anemia in order to optimize their fibrinogenemia levels immediately after treatment.

The aim of the invention is to improve the optimization of the level of fibrinogenemia in newborn piglets with anemia.

The essence of the proposed method lies in the fact that to optimize the level of fibrinogenemia, newborn piglets with anemia are prescribed ferroglukin 75 mg (1 ml) intramuscularly three times with an interval of 5 days, polyzone 3 mg / kg and crezacin 3 mg / kg when they are included in the vaping scheme for 12 days.

The method allows to optimize the level of fibrinogenemia in newborn piglets with anemia immediately after treatment, translating fibrinogenemia to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to subsequently maintain the level of fibrinogenemia in optimal condition, which will allow newborn piglets with anemia to significantly reduce the risk of thrombotic complications.

The inventive method is as follows. Hyperfibrinogenemia is established in the examined piglet by the following method (Barkagan Z.S., Momot A.P. Basics of the diagnosis of hemostatic disorders. M, 1999. - P.84-86).

The following reagents are used: 1. Phosphate buffer, pH 7.0 (0.06 M) of the following composition: 190 ml 0.5 M KH ₂ PO ₄ (68.045 g per 1 liter) and 110 ml 0.5 N sodium hydroxide. For operation, 20 ml of this basic buffer solution is brought to a volume of 100 ml with distilled water. 2. 0.15M sodium chloride solution. 3. 0.04 M solution of monoiodoacetic acid in 0.06 M phosphate buffer (40 mg of monoiodoacetic acid in 5 ml of buffer). 4. Pig thrombin, 50 NIH units / ml. 5. 1.5% solution of acetic acid.

Applied equipment. Spectrophotometer with optical density measurement in the ultraviolet zone.

The material for the study is citrate, platelet-poor plasma.

1.6 ml of 0.06 M phosphate buffer was added to a test tube with 0.2 ml of plasma and placed in a water bath (+ 37 ° C). After a few minutes, 0.1 ml of a 0.04 M solution of monoiodoacetic acid was added to the mixture, and after 3 minutes, 0.1 ml of a thrombin solution was added. After adding each component, the contents of the tube are thoroughly mixed with a glass rod (with a rough surface at the end), leaving it in the tube. The resulting mixture is incubated at + 37 ° C for at least 15 minutes (longer incubation is allowed). The resulting fibrin clot is removed by winding onto a stick, and squeezed against the wall of the tube. The clot is washed twice in 50 ml of a 0.15 M sodium chloride solution. Then washed once in chilled distilled water. Water from the surface of the clot is additionally removed with filter paper. The resulting clot is dissolved with stirring in 5 ml of 1.5% acetic acid at room temperature. Dissolution complete in 5 minutes. The protein content in the solution is determined spectrophotometrically by measuring the absorbance at 280 and 320 nm. The extinction is stable, the measurement time can be arbitrarily selected.

The concentration of fibrinogen (X) in the test plasma sample is calculated by the formula:

where E (280-320) is the difference between the extinction values at 280 and 320 nm, thereby achieving the necessary correction for turbidity; 255 — coefficient for expressing the amount of fibrinogen in g / l in an analysis performed with 0.2 ml of plasma; 15,067 is the extinction coefficient for fibrinogen in an acidic medium at a wavelength of 280 nm (E 1% 1 cm).

Normally, the fibrinogen content determined by this method is 2.0-4.0 g / l.

When newborn piglets with hyperfibrinogenemia anemia are found, they are prescribed therapy according to the claimed method - they are prescribed ferroglukin 75 mg (1 ml) intramuscularly three times with an interval of 5 days, polyzone 3 mg / kg and crezacin 3 mg / kg when they are included in the watering schedule for 12 days.

Example. In a newborn pig with anemia No. 23, the amount of hemoglobin was 78.0 g / l, erythrocytes 3.99 × 10 ¹² / l, siderocytes 0.8% with a serum iron level of 12.0 μmol / l with an increase in the total iron binding capacity of blood serum up to 69.9 μmol / l, hyperfibrinogenemia level of 8 g / l.

The piglet was prescribed 75 mg ferroglukin (1 ml) intramuscularly three times with an interval of 5 days, polyzone 3 mg / kg and crezacin 3 mg / kg when they were included in the 12-day rehydration schedule.

The piglet was examined regularly during and after treatment. At the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 118.5 g / l, erythrocytes to 5.03 × 10 ¹² / l, siderocytes to 8.9% with an increase in serum iron to 22.6 μmol / l and a decrease in the total iron binding capacity blood serum up to 35.2 μmol / l, hyperfibrinogenemia was not detected (1.5 g / l).

Using the proposed method for optimizing the level of fibrinogenemia in biology and veterinary medicine will help to avoid vascular complications in newborn piglets with anemia, optimize their microcirculation and trophic tissue, accelerating the growth of animals, improving the herd, which will allow to obtain more high-quality meat products and subsequently healthy offspring from these animals .

Claims (1)

A method of normalizing the severity of fibrinogenemia in newborn piglets with anemia by applying ferroglukin 75 mg (1 ml) intramuscularly, three times with an interval of 5 days, polyzone 3 mg / kg and crezacin 3 mg / kg when they are included in the 12-day watering schedule.