RU2399367C1 - Method of thrombin clotting time optimisation for newborn piglets with anaemia - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention refers to medicine and veterinary science. The method involves double intramuscular introduction of ferroglucin 150 mg (2 ml), every 10 days. Polyzone 4 mg/kg and cresacin 4 mg/kg are included in the feed regimen for 8 days.

EFFECT: method allows avoiding vascular complications in newborn piglets with anaemia, improving swine herd, reducing mortality and increasing amount and quality of meat production.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for normalizing trombone time in newborn piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, polyisone, a phosphoric acid salt of 2-amino-4-methylthio- (S-oxo-S-amino) -butyric acid, is a poorly soluble white to cream-colored powder in water (Ovchinnikov A) as effective stimulants of the vital activity of a growing organism in modern veterinary medicine. . and others. Polizon is a growth stimulator. // Poultry farming, 2006. - No. 12. - p.14-15.), and crezacin is a derivative of aroxyalkylcarboxylic acids. It is a white crystalline powder, readily soluble in water, with a faint specific smell, sweetish-bitter taste. It exhibits an effective stimulating effect on living organisms, increasing the survival of humans and animals in adverse and extreme conditions, stimulating or normalizing life processes (Baymatov V.N., Latypov M.M.The effect of krezacin on the state of birds. Veterinary Medicine, 2006, No. 11, p. 49-51).

However, never before has a treatment complex consisting of ferroglukin, polysone and crezacin been used in newborn piglets with anemia in order to normalize thrombin time.

The aim of the invention is to increase the correction efficiency of thrombin time in newborn piglets with anemia.

The essence of the proposed method lies in the fact that to optimize thrombin time, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg included in the scheme 8 days.

The method allows to normalize the thrombin time in newborn piglets with anemia 3 days after completion of treatment, translating it to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to maintain the optimum thrombin time in the future, which will allow newborn piglets with anemia to significantly reduce the risk of thrombotic complications, improve the herd by improving trophism of their tissues and, as a result, accelerate growth, increase the amount of meat products received and reduce case.

The inventive method is as follows.

For diagnosis in newborn piglets with anemia of a violation of thrombin time, its assessment is carried out according to the following method.

Principle of the method: determine the clotting time of plasma under the influence of thrombin, standardized for activity on the control normal plasma. Thrombin test characterizes the kinetics of the final stage of blood coagulation - the rate of conversion of fibrinogen to fibrin.

Reagents used:

1. Tris buffer, 0.05M (or Michaelis buffer), pH 7.3-7.4.

2. A solution of thrombin in the buffer activity of 14-16 seconds. The working solution of the reagent is prepared in a plastic or silicone tube and used on the day of the study. It is stored at room temperature.

The material for the study is citrate BTP.

To 0.2 ml of the test plasma, heated in a water bath at + 37 ° C for 1 min, add 0.2 ml of a thrombin solution (which should have a temperature of no higher than + 18-25 ° C before the study). Start the stopwatch immediately and note the coagulation time.

The determination is carried out manually or on a coagulometer.

The result is expressed in seconds, comparing the clotting time in the control normal (reference) and the studied plasma. Normally, thrombin time (TV) is 14-16 seconds. With anemia in piglets, an acceleration of TV is noted.

When accelerating TB in newborn piglets with anemia, it is necessary to prescribe a treatment according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg included in the 8-day watering schedule .

Example. In a newborn piglet No. 21 with anemia, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 78.6 g / l, red blood cells 4.09 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.1 μmol / l with an increase in the total iron binding capacity of blood serum to 69, 7 μmol / L. The examination revealed an acceleration of thrombin time (12 s). The piglet was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg, included in the 8-day vaping schedule.

3 days after the end of treatment, the red blood returned to normal in the piglet — the amount of hemoglobin in it was 122.0 g / l, erythrocytes 5.12 × 10 ¹² / l, siderocytes 8.6% with a serum iron level of 21.3 μmol / l with a decrease in the total iron binding capacity of blood serum to 32.3 μmol / L, a complete normalization of thrombin time was achieved (15 s), which was stably maintained in the future.

Using the proposed method for optimizing thrombin time in biology and veterinary medicine will help to avoid vascular complications in newborn piglets with anemia, improve the herd, reduce mortality and increase the volume and quality of meat products and get healthy offspring from these animals.

Claims (1)

A method for optimizing the level of thrombin time in newborn piglets with anemia, including the use of ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, polyzone 4 mg / kg and crezacin 4 mg / kg included in the 8-day vaping schedule.