RU2403025C1 - Method of thrombin time normalisation in anemia of newborn calves and piglets - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention refers to medicine and veterinary science. The method involves the introduction of ferroglucin by 150 mg (2 ml) intramuscularly, two times every 10 days, phosprenyl intramuscularly, two times by different syringes in different points, at first 0.09 ml/kg combined with an iron preparation, and for the second time in dosage 0.08 ml/kg five days after the first injection of ferroglucin and cresacine 4 mg/kg that is included in the feeding schedule for 8 days starting with the first injection of ferroglucin.

EFFECT: method allows avoiding vascular complications in newborn calves and piglets with anaemia, improving a herd, reducing mortality and increasing amount and quality of meat production, producing a healthy posterity.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for normalizing thrombin time in newborn calves and piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in calves and piglets for anemia (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as an effective stimulant of the vital activity of a growing organism. Phosprenyl is a drug based on polyprenol phosphates obtained from needles (Deeva A.V., Salakhova Z.A., Lobova T.P. et al. Improving the safety and productivity of piglets when used fosprenil and gamavita // Veterinary medicine. - 2006. - No. 4. - p.13-15), and crezacin (a derivative of aroxyalkylcarboxylic acids), which is an effective stimulant for living organisms, increasing the survival of animals in adverse and extreme conditions, stimulating or normalizing life processes (Baimatov VN, Latypov MM The effect of krezacin on the state of the bird. Veterinary medicine, 2006, No. 11, p. 49-51).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and crezacin been used in newborn calves and piglets with anemia in order to normalize thrombin time.

The aim of the invention is to increase the correction efficiency of thrombin time in newborn calves and piglets with anemia.

The essence of the proposed method lies in the fact that to optimize thrombin time, newborn calves and piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0, 09 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, and crezacin 4 mg / kg when it was included in the 8-day rehydration schedule, starting simultaneously with the first injection of ferroglukin.

The method allows to normalize the thrombin time in newborn calves and piglets with anemia 3 days after completion of treatment, translating it to a level close to that for healthy calves and piglets. Subject to the recommendations of the proposed method, it is possible to maintain the optimum thrombin time in the future, which will allow newborn calves and piglets with anemia to significantly reduce the risk of thrombotic complications, improve the herd by improving trophism and their tissues, and, as a result, accelerate growth and increase the amount received meat products and reduce the case.

The inventive method is as follows.

For the diagnosis of newborn calves and piglets with anemia of a violation of thrombin time, it is evaluated according to the following method.

Principle of the method: determine the clotting time of plasma under the influence of thrombin, standardized for activity on the control normal plasma. Thrombin test characterizes the kinetics of the final stage of blood coagulation - the rate of conversion of fibrinogen to fibrin.

Reagents used: 1. Tris buffer, 0.05 M (or Michaelis buffer), pH 7.3-7.4. 2. A solution of thrombin in a buffer with an activity of 14-16 s. The working solution of the reagent is prepared in a plastic or silicone tube and used on the day of the study. It is stored at room temperature.

The material for the study is citrate BTP.

To 0.2 ml of the test plasma, heated in a water bath at + 37 ° C for 1 min, add 0.2 ml of a thrombin solution (which should have a temperature of no higher than + 18-25 ° C before the study). Start the stopwatch immediately and note the coagulation time.

The determination is carried out manually or on a coagulometer.

The result is expressed in seconds, comparing the clotting time in the control normal (reference) and the studied plasma. Normal thrombin time (TB) is 14-16 s. With anemia in calves and piglets, an acceleration of TV is noted.

When accelerating TB in newborn calves and piglets with anemia, it is necessary to prescribe treatment according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, twice in different syringes at different points, the first time 0.09 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, and crezacin 4 mg / kg when it is included in the 8-day watering schedule, starting simultaneously with the first injection of ferroglukin.

Example 1. In a newborn piglet No. 19 with anemia, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 80.0 g / l, red blood cells 4.02 × 10 ¹² / l, siderocytes 0.9% with a serum iron level of 12.1 μmol / l with an increase in the total iron binding capacity of blood serum to 67, 8 μmol / L. The examination revealed an acceleration of thrombin time (12 s). Piglet was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.09 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, and crezacin 4 mg / kg when it is included in the 8-day drinking schedule, starting simultaneously with the first injection of ferroglukin.

3 days after the end of treatment, the red blood returned to normal in the piglet — the amount of hemoglobin in it was 128.0 g / l, erythrocytes 5.20 × 10 ¹² / l, siderocytes 8.1% with a serum iron level of 23.0 mmol / l with a decrease in the total iron binding capacity of blood serum to 30.4 μmol / l. A complete normalization of thrombin time was achieved (15 s), which was stably preserved in the future.

Example 2. In a newborn calf No. 28 with anemia, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 81.0 g / l, erythrocytes 4.10 × 10 ¹² / l, siderocytes 0.9% with a serum iron level of 12.3 μmol / l with an increase in the total iron binding capacity of blood serum to 69, 2 μmol / L. The examination revealed an acceleration of thrombin time (11 s). The calf was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.09 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, and crezacin 4 mg / kg when it is included in the 8-day drinking schedule, starting simultaneously with the first injection of ferroglukin.

3 days after the end of treatment, the calf returned to normal red blood - the amount of hemoglobin in it was 129.0 g / l, red blood cells 5.26 × 10 ¹² / l, siderocytes 8.6% with a serum iron level of 24.0 μmol / l with a decrease in the total iron binding capacity of blood serum to 29.1 μmol / l. A complete normalization of thrombin time was achieved (16 s), which was stably preserved in the future.

Using the proposed method for optimizing thrombin time in biology and veterinary medicine will help to avoid vascular complications in newborn calves and piglets with anemia, improve the herd, reduce mortality and increase the volume and quality of meat products and get healthy offspring from these animals.

Claims (1)

A method of normalizing trombone time in newborn calves and piglets with anemia, including the use of ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, twice in different syringes at different points, the first time 0.09 ml / kg at a time with an iron preparation, a second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 8-day watering schedule, starting simultaneously with the first injection of ferroglukin.