RU2349309C1 - Method of thrombin time stabilisation for newborn piglets with anaemia - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: method involves prescription of intramuscular injection of ferroglucinum by 150 mg (2 ml) twice over 10 day interval, intramuscular injection of phosprenyl thrice by different syringes into different points, by 0.08 ml/kg for the first time together with iron agent, by 0.07 ml/kg over five days after the first injection, by 0.05 ml/kg for the third time together with the second injection. Additionally, 0.016 ml/kg of gamavit is injected intramuscularly once per day for six days beginning from the first ferroglucinum injection.

EFFECT: possible thrombin time stabilisation for newborn piglets with anaemia.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for normalizing trombone time in newborn piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M.: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as effective stimulants of the vital activity of a growing organism in phosprenyl, a drug based on polyprenol phosphates obtained from needles, and gamavit, a complex balanced means based on the placenta, containing sodium nucleate, a set of amino acids, vitamins, and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita. // Veterinary Medicine. - 2006. - No. 4. - p.13-15).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn piglets with anemia in order to normalize thrombin time.

The aim of the invention is to increase the correction efficiency of thrombin time in newborn piglets with anemia.

The essence of the proposed method lies in the fact that to optimize thrombin time, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, three times in different syringes at different points, the first time 0.08 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and gamavit 0.016 ml / kg intramuscularly once a day, six days, n starting with the first injection of ferroglukin.

The method allows to normalize the thrombin time in newborn piglets with anemia 5 days after completion of treatment, translating it to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to maintain the optimal thrombin time in the future, which will allow newborns with anemia to significantly reduce the risk of thrombotic complications, improve the herd by improving trophism and their tissues, and as a result accelerate growth, increase the amount of meat products received and reduce case.

The inventive method is as follows.

For diagnosis in newborn piglets with anemia of a violation of thrombin time, its assessment is carried out according to the following method.

Principle of the method: determine the clotting time of plasma under the influence of thrombin, standardized for activity on the control normal plasma. Thrombin test characterizes the kinetics of the final stage of blood coagulation - the rate of conversion of fibrinogen to fibrin.

The following reagents are used: 1. Tris buffer, 0.05 M (or Michaelis buffer), pH 7.3-7.4. 2. A solution of thrombin in the buffer activity of 14-16 seconds. The working solution of the reagent is prepared in a plastic or silicone tube and used on the day of the study. It is stored at room temperature.

The material for the study is citrated BTP.

To 0.2 ml of the test plasma, heated in a water bath at + 37 ° C for 1 min, add 0.2 ml of a thrombin solution (which should have a temperature not higher than + 18-25 ° C before the study). Start the stopwatch immediately and note the coagulation time.

The determination is carried out manually or on a coagulometer.

The result is expressed in seconds, comparing the clotting time in the control normal (reference) and the studied plasma. Normally, thrombin time (TV) is 14-16 seconds. With anemia in piglets, acceleration of TV is noted.

When accelerating TB in newborn piglets with anemia, it is necessary to prescribe treatment according to the claimed method: ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, the first time 0.08 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and gamavit 0.016 ml / kg intramuscularly once a day, six days starting with the first injection f erroglyukin.

Example. In a newborn piglet No. 11 with anemia, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 82.0 g / l, erythrocytes 4.05 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.0 μmol / l with an increase in the total iron binding capacity of blood serum to 68, 2 μmol / L. The examination revealed an acceleration of thrombin time (12 seconds). Piglet was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0.08 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.05 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.016 ml / kg intramuscularly once a day, six days, starting with the first injection of ferroglukin.

5 days after the end of the treatment, the red blood returned to normal in the piglet - its hemoglobin count was 118.0 g / l, erythrocytes 5.10 × 10 ¹² / l, siderocytes 8.0% with a serum iron level of 21.0 μmol / l with a decrease in the total iron-binding ability of blood serum to 35.9 μmol / L, a complete normalization of thrombin time was achieved (15 sec), which stably remained in the future.

Using the proposed method for optimizing thrombin time in biology and veterinary medicine will help to avoid vascular complications in newborn piglets with anemia, improve the herd, reduce mortality and increase the volume and quality of meat products and get healthy offspring from these animals.

Claims (1)

A method for normalizing thrombin time in newborn piglets with anemia, including the use of ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, the first time 0.08 ml / kg simultaneously with the drug iron, a second time at a dose of 0.07 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and hamavit 0.016 ml / kg intramuscularly once a day, six days, starting with the first ferroglu injection ins.