RU2349308C1 - METHOD OF α2 ANTIPLASMIN LEVEL STABILISATION FOR NEWBORN PIGLETS WITH ANAEMIA - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: method involves prescription of intramuscular injection of ferroglucinum by 150 mg (2 ml) twice over 10 day interval, intramuscular injection of phosprenyl thrice by different syringes into different points, by 0.07 ml/kg for the first time together with iron agent, by 0.06 ml/kg over five days after the first injection, by 0.05 ml/kg for the third time together with the second injection. Additionally, 0.012 ml/kg of gamavit is injected intramuscularly once per day for ten days beginning from the first ferroglucinum injection.

EFFECT: possible alpha 2 antiplasmin level stabilisation for newborn piglets with anaemia.

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Description

The invention relates to biology and veterinary medicine, namely to hematology.

Analogues of the proposed method for normalizing the level of α ₂ antiplasmin in newborn piglets with anemia does not exist.

There is evidence of the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as effective stimulants of the vital activity of a growing organism in phosprenyl, a drug based on polyprenol phosphates obtained from needles, and gamavit, a complex balanced means based on the placenta, containing sodium nucleate, a set of amino acids, vitamins, and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita. // Veterinary Medicine. - 2006. - No. 4. - p.13-15).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn piglets with anemia in order to normalize the level of α ₂ antiplasmin.

The aim of the invention is to increase the efficiency of correction of the level of α ₂ antiplasmin in newborn piglets with anemia.

The essence of the proposed method lies in the fact that to optimize the level of α ₂ antiplasmin, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0, 07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.06 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.05 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.012 ml / kg intramuscularly once a day, eight days Starting with the first ferroglyukina injection.

The method allows to normalize the level of α ₂ antiplasmin in newborn piglets with anemia 5 days after completion of treatment, translating it to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to maintain the subsequent activity level of α ₂ antiplasmin in optimal condition, which will allow newborn piglets to significantly reduce the risk of thrombotic complications, improve the herd, increase weight gain, reduce mortality and improve the quality and volume of meat products.

The inventive method is as follows.

For diagnosis in newborn piglets with anemia of violation of the level of α ₂ antiplasmin, its assessment is carried out by the following method.

α ₂ Antiplasmin (α ₂ -AP) is the most important inhibitor of fibrinolysis, in the presence of which the rapid formation of an irreversible, deprived of activity complex "plasmin-α ₂ -AP" occurs. The method is based on the determination of the ability of the α ₂ -AP of the studied plasma sample to inactivate the purified plasmin added to the plasma. The content of residual plasmin is determined by the degree of hydrolysis of the chromogenic substrate specific for this enzyme.

α ₂ -AP- _sample + plasmin _excess = [α ₂ -AP-plasmin] + plasmin _residue

Reagents are used: 1. Pig plasmin, lyophilized. It is diluted in buffer to a concentration of 0.1 CTA U / ml. 2. Chromogenic substrate D-Norvalylcyclohexylalanil-lysil-p-nitroanilide. It is diluted in distilled water to a concentration of 3 mmol / L. 3. The buffer solution: KN ₂ PO ₄ (100 mmol / l), NaCl 9 g / l, glycerin (250 g / l), pH 7.5.

To carry out the study, standard or control normal plasma with a known content of α ₂ -AP or pooled (taken from at least 10 healthy piglets), the activity of α ₂ -AP in which is taken as 100%, is also required.

The following equipment is used: spectrophotometer with a thermostatically controlled cell. The reaction temperature is + 37 ° C. The used wavelength is 405 nm. The width of the optical path of the cell is 1 cm.

The material for the study is a citrate platelet-poor plasma.

Previously, all mixed reagents are heated to + 37 ° C. The course of determination is given in the table.

Table.
The scheme for determining the level of α ₂ antiplasmin in the subject Sequence of operations Test sample Control (plasmin) 1. Pour into a cuvette or test tube: test plasma 20 μl (0.02 ml) - physiological (0.15 M) sodium chloride solution - 20 μl (0.02 ml) plasmin solution 1.0 ml 1.0 ml 2. Stir and incubate at + 37 ° C for 1 min 3. Add a chromogenic substrate and turn on the stopwatch 0.1 ml 0.1 ml Within 30 seconds, absorption indicators are taken into account and at the same time a stopwatch is started. After 60 and 120 seconds, absorbance values were re-determined.

The rate of change in absorption over 1 min (ΔA / min), which is proportional to the activity of α ₂ -AP, is calculated. To do this, calculate the difference in absorption at 405 nm between 0 and 60 seconds of incubation, as well as between 60 and 120 seconds. The average value is determined from two indicators ΔA / min.

Each series of analyzes requires at least one value for the plasmin solution (ΔA / min _plasmin ) and one reference value determined using the control normal plasma (ΔA / min _control ) with a known value of α ₂ -AP. These values are used to calculate the intralaboratory conversion factor (K _p ):

The content of α ₂ -AP in the studied plasma sample in the form of% of the norm is further calculated by the formula: α ₂ -AP _sample (% of the norm) = Kp × (ΔA / min _plasmin -ΔA / min _sample )

Normally, the level of α ₂ -AP in the blood plasma is 80-120%.

A decrease in the activity of α ₂ -AP can be determined by activation of fibrinolysis, its increase is associated with a risk of thrombosis.

When diagnosing newborn piglets with anemia of an increase in α ₂ -AP, it is necessary to prescribe treatment according to the claimed method.

The essence of the proposed method lies in the fact that to optimize the level of α ₂ antiplasmin, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0, 07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.06 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.05 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.012 ml / kg intramuscularly once a day, eight days Starting with the first ferroglyukina injection.

Example 1. Blood was taken from a newborn piglet with anemia No. 18 and examined according to the above scheme.

In this piglet, a violation of erythropoiesis was noted with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 83.0 g / l, red blood cells 4.12 × 10 ¹² / l, siderocytes 1.2% with an iron level in serum of 13.2 μmol / l with an increase in the total iron binding capacity of blood serum to 68, 2 μmol / L.

Examination of the pig showed an increased activity of α ₂ -AP (146%). The animal was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days.

5 days after the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 116.0 g / l, erythrocytes to 5.00 × 10 ¹² / l, siderocytes to 7.2% with an increase in serum iron to 20.4 μmol / l and a decrease in total iron binding capacity blood serum up to 39.6 μmol / l. However, an incomplete decrease in α ₂ -AP activity (128%) was achieved in the piglet, which did not reach normal values.

Subsequent monitoring of the piglet subsequently recorded a weak positive dynamics of α ₂ -AP - 1 month after the end of treatment, α ₂ -AP was 124%.

Example 2. Blood was taken from a newborn piglet with anemia No. 19 and examined according to the above scheme.

In this piglet, a violation of erythropoiesis was noted with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 82.0 g / l, erythrocytes 4.10 × 10 ¹² / l, siderocytes 1.0% with a serum iron level of 12.6 μmol / l with an increase in the total iron binding capacity of blood serum to 68, 9 μmol / L.

Examination of the pig revealed an increased activity of α ₂ -AP (145%). The animal was prescribed 150 mg ferroglukin intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.06 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.05 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.012 ml / kg intramuscularly once a day, eight days, starting with the first injection of ferroglukin.

5 days after the end of treatment, the red blood returned to normal in the piglet.

So, the amount of hemoglobin in him increased to 120.6 g / l, erythrocytes - up to 5.33 × 10 ¹² / l, siderocytes up to 8.4% with an increase in iron in serum to 28.4 μmol / l and a decrease in total iron binding capacity blood serum up to 33.6 μmol / l.

A complete normalization of α ₂ -AP activity was achieved in the piglet (89%).

Subsequent monitoring of the piglet subsequently did not record a negative dynamics of α ₂ -AP.

Using the proposed method for optimizing the activity of α ₂ -AP in veterinary medicine will help to avoid vascular disorders in newborn piglets with anemia, improve the herd, reduce mortality, increase weight gain, improve the quality and volume of meat products and get healthy offspring from these piglets.

Claims (1)

A method for normalizing the level of α ₂ antiplasmin in newborn piglets with anemia by applying ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, three times in different syringes at different points, the first time 0.07 ml / kg simultaneously with iron preparation, a second time at a dose of 0.06 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and gamavit 0.012 ml / kg intramuscularly once a day, eight days starting with the first ferroglukin injection .