RU2472495C1 - Method for normalising alpha2-antiplasmin level in sows with bronchitis - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: declared invention relates to veterinary science. A method involves the use of crezacin 4 mg/kg a day of fed-on plan in the morning for 5 days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for 5 days.

EFFECT: use of the declared method enables avoiding developing vascular thrombosis in sows with bronchitis, sanitising a flock, reducing death loss, producing high-quality meat products from these animals.

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Description

The invention relates to biology and veterinary medicine, namely to hematology.

Analogues of the proposed method for normalizing the level of α ₂ -antiplasmin in sows with bronchitis does not exist.

There is information in the literature on the high biological activity of crezacin, a derivative of aroxyalkylcarboxylic acids, capable of stimulating various systems of living organisms, increasing their survival under adverse conditions (Baimatov V.N., Latypov M.M. . - No. 11. - S.49-51), and gamavita - a complex balanced means based on the placenta containing sodium nucleinate, a set of amino acids, vitamins and salts, which has a high organoprotective and anabolic effect (Bel Usov RV Application gamavita to improve the efficiency of reproduction of pigs // Veterinary Medicine - 2006. - №10 -. S.11-12). However, in the literature there is no indication of the effect of these drugs on SAE in sows with arthritis, and even more so on the possibility of normalizing it using a combination of them.

The aim of the invention is the normalization of the level of α ₂ -antiplasmin in sows with bronchitis.

The essence of the proposed method lies in the fact that to normalize the level of α ₂ -antiplasmin in sows with bronchitis, they are prescribed crezacin 4 mg / kg per day, which is included in the feeding schedule for five days in the morning, and at the same time gamavit 0.03 mg / kg intramuscularly once a day, in the morning, for five days.

The use of the claimed complex normalizes the level of α ₂ -antiplasmin in sows with bronchitis 5 days after the end of treatment, eliminating the effects of bronchitis. This is the basis for improving the herd of sows, reducing the mortality and high quality of piglets and meat products received from them.

The inventive method is as follows.

It is known that α ₂ -antiplasmin (α ₂ -AP) is the most important inhibitor of fibriolysis, in the presence of which there is a rapid formation of an irreversible, depleted plasmin-α ₂ -AP complex. The method for assessing the level of α ₂ -AP is based on determining the ability of the α ₂ -AP to study the plasma sample to inactivate the purified plasmin added to the plasma. The content of residual plasmin is determined by the degree of hydrolysis of the chromogenic substrate specific for this enzyme.

Reagents are used: 1. Pig plasmin, lyophilized. It is diluted in buffer to a concentration of 0.1 CTA U / ml. 2. Chromogenic substrate D-Norvalylcyclohexylalanil-lysil-p-nitroanilide. It is diluted in distilled water to a concentration of 3 mmol / L. 3. Buffer solution: KH ₂ PO ₄ (100 mmol / L), NaCl 9 g / L, glycerin (250 g / L), pH 7.5.

To perform the study, standard or control normal plasma with a known content of α ₂ -AP or pooled (taken from at least 10 healthy sows), the activity of α ₂ -AP in which is taken as 100%, is also required.

The following equipment is used: spectrophotometer with a thermostatically controlled cell (reaction temperature + 37 ° C). The used wavelength is 405 nm. The width of the optical path of the cell is 1 cm.

The material for the study is nitrate-poor platelet plasma.

Previously, all mixed reagents are heated to + 37 ° C. The progress of the determination is given in the table below.

Table Scheme for determining the level of α ₂ -antiplasmin Sequence of operations Test sample Control (plasmin) 1. Pour into a cuvette or test tube: test plasma 20 μl (0.02 ml) - physiological (0.15 M) sodium chloride solution - 20 μl (0.02 ml) plasmin solution 1.0 ml 1.0 ml 2. Stir and incubate at + 37 ° C for 1 min 3. Add a chromogenic substrate and turn on the stopwatch 0.1 ml 0.1 ml Within 30 seconds, absorption indicators are taken into account and at the same time a stopwatch is started. After 60 and 120 seconds, absorbance values were re-determined.

The rate of change in absorption over 1 min (Δ A / min), which is proportional to the activity of α ₂ -AP, is calculated. To do this, calculate the difference in absorption at 405 nm between 0 and 60 seconds of incubation, as well as between 60 and 120 seconds. Determine the average value of the two indicators Δ A / min

Each series of analyzes requires at least one value for a plasmin solution (Δ A / min _plasmin ) and one reference value determined using the control normal plasma (Δ A / min _control ) with a known value of α ₂ -AP. These values are used to calculate the intralaboratory conversion factor (K _p ):

The content of α ₂ -AP in the studied plasma sample in the form of% of the norm is further calculated by the formula:

α ₂ -AP _sample (% of normal) = Kp × (Δ A / min _plasmin - Δ A / min _sample )

Normally, the level of α ₂ -AP in the blood plasma is 80-120%.

A decrease in the activity of α ₂ -AP can be determined by activation of fibrinolysis, its increase is associated with a risk of thrombosis.

When diagnosing an increase in α ₂ -AP in sows with bronchitis, it is necessary to prescribe treatment according to the claimed method.

Example 1. Blood was taken from a sow with bronchitis No. 19 and examined according to the above scheme.

Examination of the sow revealed an increased activity of α ₂ -AP (147%). The animal was prescribed krezacin 4 mg / kg per day, included in the five-day morning feeding schedule, and at the same time, gamavit 0.03 mg / kg intramuscularly once a day, in the morning, for five days.

5 days after the end of treatment, the activity of α ₂ -AP (128%) was normalized in the sow with the elimination of bronchitis.

Subsequent monitoring of the sow subsequently recorded persistent maintenance of the normal level of α ₂ -AP for at least 3 months (follow-up) after treatment - α ₂ -AP not higher than 92%.

Example 2. Blood was taken from a sow with bronchitis No. 24 and examined according to the above scheme. She revealed an increase in the activity of α ₂ -AP (136%). The animal was prescribed krezacin 4 mg / kg per day, included in the five-day morning feeding schedule, and at the same time, gamavit 0.03 mg / kg intramuscularly once a day, in the morning, for five days.

5 days after the end of treatment, the activity of α ₂ -AP (86%) was normalized in the sow with the elimination of bronchitis.

Subsequent observation of the sow subsequently did not record negative dynamics of α ₂ -AP (within 3 months of observation).

Using the proposed method for optimizing the activity of α ₂ -AP in veterinary medicine can help to avoid the development of vascular thrombosis in sows with bronchitis, which allows to improve the herd, reduce mortality and receive high-quality meat products from these animals.

The application of the claimed method was successfully tested in 126 sows with bronchitis with the achievement of full normalization of the level of α ₂ -antiplasmin in all cases.

Claims (1)

A method for normalizing the level of α ₂ -antiplasmin in sows with bronchitis, including the use of crezacin 4 mg / kg per day in the morning route for five days and simultaneously with gamavita 0.03 mg / kg intramuscularly once a day in the morning for five days.