RU2392927C1 - Method of normalising antiplasmin level in anemia of newborn calves and pigs - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention relates to veterinary science. The method involves application of Ferroglucine 150 mg (2 ml) intramuscularly, two times every 10 days, Phosprenyl intramuscularly, two times in different syringes in different points, at first 0.07 ml/kg combined with an iron preparation, and the second time in dosage 0.07 ml/kg six days after the first injection of Ferroglucine and Cesacine 4 mg/kg that is included in the feeding schedule on the 10th day.

EFFECT: method allows normalising and maintaining optimum of α² antiplasmin level, reducing risk of thrombotic complications, improving herd, reducing mortality, increasing weight gains, improving quality and amount of produced meat.

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Description

The invention relates to biology and veterinary medicine, namely to hematology.

Analogues of the proposed method for normalizing the level of α ₂ antiplasmin in newborn calves and piglets with anemia does not exist.

There is information about the use of ferroglukin in calves and piglets for anemia (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991. - 576 p.).

As modern stimulants of the vital activity of a growing organism in modern veterinary medicine, phosprenyl is used - a drug based on polyprenol phosphates obtained from needles (Deeva A.V., Salakhova Z.A., Lobova T.P. et al. Improving the safety and productivity of piglets when used fosprenil and gamavita. // Veterinary medicine. - 2006. - No. 4. - p.13-15), and crezacin (a derivative of aroxyalkylcarboxylic acids). Krezacin is a white crystalline powder, readily soluble in water, with a faint specific smell, sweetish-bitter taste. It exhibits an effective stimulating effect on living organisms, increasing the survival of animals in adverse and extreme conditions, stimulating or normalizing life processes (Baimatov V.N., Latypov M.M. Effect of krezacin on the state of birds. Veterinary Medicine, 2006, No. 11, p. 49-51).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and crezacin been used in newborn calves and piglets with anemia in order to normalize the level of α ₂ antiplasmin.

The aim of the invention is to increase the efficiency of correction of the level of α ₂ antiplasmin in newborn calves and piglets with anemia.

The essence of the proposed method lies in the fact that to optimize the level of α ₂ antiplasmin newborn calves and piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day rehydration schedule.

The method allows to normalize the level of α ₂ antiplasmin in newborn calves and piglets with anemia 3 days after completion of treatment, translating it to a level close to that for healthy calves and piglets. Subject to the recommendations of the proposed method, it is possible to maintain the subsequent activity level of α ₂ antiplasmin in optimal condition, which will allow newborn calves and piglets to significantly reduce the risk of thrombotic complications, improve the herd, increase weight gain, reduce mortality and improve the quality and volume of meat products.

The inventive method is as follows.

For the diagnosis in newborn calves and piglets with anemia of a violation of the α ₂ level _of antiplasmin, its assessment is carried out according to the following method (Barkagan Z.S., Momot A.P. Basics of the diagnosis of hemostasis disorders. M., 1999).

α ₂ antiplasmin (α ₂ -AP) is the most important inhibitor of fibrinolysis, in the presence of which the rapid formation of an irreversible, deprived of the activity of the complex "plasmin-α ₂ -AP" occurs. The method is based on the determination of the ability of the α ₂ -AP of the studied plasma sample to inactivate the purified plasmin added to the plasma. The content of residual plasmin is determined by the degree of hydrolysis of the chromogenic substrate specific for this enzyme.

α ₂ -AP- _sample + plasmin _excess = [α ₂ -AP-plasmin] + plasmin _residue

The following reagents are used: 1. Pig or bull plasmin, lyophilized, respectively. It is diluted in buffer to a concentration of 0.1 CTA U / ml. 2. Chromogenic substrate of D-Norvalylcyclohexylalananisil-p-nitroanilide. It is diluted in distilled water to a concentration of 3 mmol / L. 3. Buffer solution: KH ₂ PO ₄ (100 mmol / L), NaCl 9 g / L, glycerin (250 g / L), pH 7.5.

To perform the study, a standard or control normal plasma with a known content of α ₂ -AP or pooled (taken from at least 10 healthy calves or piglets, respectively), the activity of α ₂ -AP in which is taken as 100%, is also required.

The following equipment is used: spectrophotometer with a thermostatically controlled cell. The reaction temperature is + 37 ° C. The used wavelength is 405 nm. The width of the optical path of the cell -1 cm.

The material for the study is a citrate platelet-poor plasma.

Previously, all mixed reagents are heated to + 37 ° C. The course of determination is given in the table.

Table. The scheme for determining the level of α ₂ antiplasmin Sequence of operations Test sample Control (plasmin) 1. Pour into a cuvette or test tube: test plasma 20 μl (0.02 ml) - physiological (0.15 M) sodium chloride solution - 20 μl (0.02 ml) plasmin solution 1.0 ml 1.0 ml 2. Stir and incubate at + 37 ° C for 1 min 3. Add a chromogenic substrate and turn on the stopwatch 0.1 ml 0.1 ml Within 30 seconds, absorption indicators are taken into account and at the same time a stopwatch is started. After 60 and 120 seconds, absorbance values were re-determined.

The rate of change in absorption over 1 min (Δ A / min), which is proportional to the activity of α ₂ -AP, is calculated. To do this, calculate the difference in absorption at 405 nm between 0 and 60 seconds of incubation, as well as between 60 and 120 seconds. Determine the average value of the two indicators Δ A / min

Each series of analyzes requires at least one value for a plasmin solution (Δ A / min _plasmin ) and one reference value determined using the control normal plasma (Δ A / min _control ) with a known value of α ₂ -AP. These values are used to calculate the intralaboratory conversion factor (K _p ):

The content of α ₂ -AP in the studied plasma sample in the form of% of the norm is further calculated by the formula:

α ₂ -AP _sample (% of normal) = Kp × (Δ A / min _plasmin - Δ A / min sample)

Normally, the level of α ₂ -AP in the blood plasma is 80-120%.

A decrease in the activity of α ₂ -AP can be determined upon activation of fibrinolysis; its increase is associated with a risk of thrombosis.

In the diagnosis of newborn calves and piglets with anemia, an increase in α ₂ -AP is necessary to prescribe treatment according to the claimed method.

Example 1

Blood was taken from a newborn piglet with anemia No. 23 and examined according to the above scheme.

This piglet showed a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 77.0 g / l, erythrocytes 3.84 × 10 ¹² / l, siderocytes 0.8% with a serum iron level of 11.0 μmol / l with an increase in the total iron binding capacity of blood serum to 72, 4 μmol / L.

Examination of the pig showed an increased activity of α ₂ -AP (146%). The animal was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day rehydration schedule.

3 days after the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 125.0 g / l, erythrocytes - to 5.24 × 10 ¹² / l, siderocytes to 7.9% with an increase in serum iron to 24.4 μmol / l and a decrease in total iron binding capacity blood serum up to 32.0 μmol / l. A complete normalization of α ₂ -AP activity (110%) was achieved in the piglet.

Observation of the piglet subsequently recorded the stability of the achieved level of α ₂ -AP, which was 1 month after the end of treatment (112%).

Example 2

Blood was taken from a newborn calf with anemia No. 34 and examined according to the above scheme.

In this calf, there was a violation of erythropoiesis with signs of a decrease in the level of iron in his body, accompanied by a change in blood counts. So, the amount of hemoglobin in him was 80.9 g / l, red blood cells 4.02 × 10 ¹² / l, siderocytes 1.1% with a serum iron level of 11.9 μmol / l with an increase in the total iron binding capacity of blood serum to 67, 4 μmol / L.

Examination of the calf revealed an increased activity of α ₂ -AP (139%). The animal was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the 10-day rehydration schedule.

3 days after the end of treatment, the calf returned to normal red blood.

So, the amount of hemoglobin in it increased to 129.0 g / l, erythrocytes - up to 5.40 × 10 ¹² / l, siderocytes up to 8.2% with an increase in serum iron to 28.0 μmol / l and a decrease in the total iron binding capacity blood serum up to 32.1 μmol / l.

The calf achieved full normalization of α ₂ -AP activity (92%).

Subsequent monitoring of the calf did not subsequently detect a negative dynamics of α ₂ -AP. After 1 month, α ₂ -AP was 93%.

Using the proposed method for optimizing the activity of α ₂ -AP in veterinary medicine will help to avoid vascular disorders in newborn calves and piglets with anemia, improve the herd, reduce mortality, increase weight gain, improve the quality and volume of meat products and subsequently receive healthy offspring from these calves and piglets .

Claims (1)

A method for normalizing the level of α ₂ antiplasmin in newborn calves and piglets with anemia, which involves the use of 150 mg ferroglukin intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, twice in different syringes at different points, the first time 0.07 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.07 ml / kg six days after the first injection of ferroglukin and crezacin 4 mg / kg when it is included in the watering schedule for 10 days.