RU2469710C1 - Method of correction of antioxidant activity of liquid part of blood of newborn calves with iron deficiency anemia - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: calves are injected intramuscularly as a single dose the solution of ferroglucine-75 on the basis of 15 mg of iron per 1 kg of live weight of a calf, and glicopin is fed 6.0 mg/day in the morning for 6 days, starting simultaneously with the injection of ferroglucine-75.

EFFECT: method enables to normalise the antioxidant activity of the liquid part of the blood of newborn calves with iron deficiency anemia, to increase the reactivity of the animal organism, to reduce the risk of thrombotic complications, promotes normal growth and development of calves.

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Description

The invention relates to biology and veterinary medicine, namely to hematology.

Analogues of the proposed method for the correction of antioxidant activity (AOA) of the liquid part of the blood in newborn calves with iron deficiency anemia does not exist.

In the literature there is information about the use of ferroglukin in anemia in calves (Internal non-communicable diseases of farm animals. Under the editorship of V.M. Danilevsky. M.: Agropromizdat, 1991. - 576 p.).

The literature also contains recommendations for use in animals for the purpose of immunostimulation of glycopine ("N-acetylglucosaminyl-N-acetyl-muramyl-alanyl-D-isoglutamine) (Andronova T.M., Pinegin B.V., Kozlov I.G., Ustinova GI The use of the glycopin immunomodulator for the prevention and treatment of animal diseases. Guidelines. Moscow, 2009. - 12 pp.) However, never before has glycopene and especially its combination with ferroglukin been used in newborn calves with iron deficiency anemia for the purpose of correction their attenuation of AOA liquid parts of the blood that can cause the generation of a huge number of free radicals, which leads to oxidative stress, a sharp deterioration in the course of the underlying disease and a weakening of the reactivity of a living organism.

The aim of the invention is the normalization of the antioxidant activity of blood fluid in newborn calves with iron deficiency anemia.

The essence of the proposed method lies in the fact that for the correction of AOA of the liquid part of the blood (plasma) in newborn calves with iron deficiency anemia, a solution of ferroglukin-75 (1 ml) containing 75 mg of iron in 1 ml of a solution of 15 mg of iron per 1 is once administered intramuscularly kg live calf body weight and glycopine vaping at 6.0 mg / day in the morning (diluted in a small amount (100.0-150.0 ml) of water or milk or colostrum intended for calves to be fed) for 6 days, starting simultaneously with injection of ferroglukin-75.

The method is as follows. The calf is taken 5.0 blood from a vein and AOA of the liquid part of the blood is evaluated (Volchegorsky I.A., Dolgushin I.N., Kolesnikov O.L., Tseilikman V.E. Experimental modeling and laboratory assessment of adaptive reactions of the body. Chelyabinsk, 2000. - 167 p.), Which is an indicator that indirectly characterizes the entire AOA of the animal's body. For these purposes, pre-prepared preparations of combined brain homogenase obtained from 5-6 rats should be used. The brain of each animal is homogenized in 10 ml of physiological saline and freed from large particles by centrifugation, followed by filtration through gauze. A mixture of the obtained supernatants is stored frozen. A preparation thus prepared is suitable for determining AOA within two weeks.

1.79 ml of isotonic sodium phosphate buffer (pH 7.4) are added to two tubes. Then, 1 ml of 28% trichloroacetic acid (TCA) with 0.1% ethylene diamine tetraacetic acid (EDTA), 0.2 ml of brain homogenate, and 0.01 ml of the studied biological fluid are successively added to one of them (control). The same amount of homogenate and test liquid are added to the second test tube (experimental), the tube is incubated under air access at 37 ° C for 60 minutes, after which the induced lipoperoxidation is stopped by adding 1 ml of TCA with EDTA.

At the same time, the oxidizability of the homogenate used is evaluated in the absence of the studied biological fluid (instead, 0.01 ml of sodium phosphate buffer is added to the test tube; otherwise, the determination does not change).

AOA is assessed by the degree of inhibition of lipid peroxidation in vitro in the presence of body fluid. The calculation is carried out according to the formula: AOA = 100% × [1- (Eo; 60-Eo; 0) / (Yk; 60-Ek; 0)], where Ek; 60 and Ek; 0 - optical density values after 60 minutes of incubation and at the “zero” point in time for samples containing the studied biological fluid, and E0; 60 and E0; 0 - the same extinction values for samples that do not contain the studied biological fluid. Normal AOA values for newborn calves range from 34.0% to 42.0%.

To correct the decreased AOA of the body in newborn calves with iron deficiency anemia, ferroglukin-75 (1 ml) is prescribed once, containing 75 mg of iron in a 1 ml solution of 15 mg of iron per 1 kg of calf body weight and 6.0 mg of glycopene / day in the morning for 6 days, starting simultaneously with the injection of ferroglukin-75.

The method allows to normalize the antioxidant activity of the liquid part of the blood in newborn calves with iron deficiency anemia on the next day after treatment, translating it to a level characteristic of healthy animals, which increases the reactivity of the animal’s body and treats the underlying disease, which significantly reduces the risk of thrombotic complications, contributing to normal growth and development of animals.

Example 1. Calf No. 116, the 3rd day of life, body weight of 27.0 kg with iron deficiency anemia was examined in a calf. Blood was taken and examined from the calf with an AOA score, which turned out to be reduced - 19%.

The calf was prescribed 5.4 ml of a solution of ferroglukin-75 intramuscularly once and drinking of glycopine diluted in 100.0 ml of milk at 6.0 mg / day in the morning for 6 days, starting simultaneously with the injection of ferroglukin-75, which allowed the next day after the end correction to raise AOA to standard values - 37.0%, eliminating the risk of its weakening in the future.

In the future, it was recommended to contain this calf on a rational diet for the prevention of relapse of iron deficiency and weakening of the AOA of the liquid part of the blood.

Example 2. Calf No. 119, the fourth day of life, body weight 31.5 kg, with iron deficiency anemia was examined in a calf. Blood was taken and examined from the calf with an AOA score, which turned out to be reduced - 27%. The calf was prescribed 6.3 ml of a solution of ferroglukin-75 intramuscularly once and drinking the glycopine milk diluted in 150.0 ml at 6.0 mg / day in the morning for 6 days, starting simultaneously with the injection of ferroglukin-75, which allowed the next day after the final correction to raise AOA to standard values - 39%, eliminating the risk of its weakening in the future.

In the future, it was recommended to contain this calf on a rational diet for the prevention of relapse of iron deficiency and weakening of the AOA of the liquid part of the blood.

The claimed method was tested on 145 newborn calves with iron deficiency anemia, showing in 100% of cases the effectiveness of the correction of iron deficiency anemia and AOA level of the liquid part of the blood.

Claims (1)

A method for correcting the antioxidant activity of the liquid part of the blood in newborn calves with iron deficiency anemia, including the use of a solution of ferroglukin-75 at the rate of 15 mg of iron per 1 kg of calf body weight intramuscularly, once in combination with the release of glycopine at 6.0 mg / day in the morning for 6 days, starting simultaneously with the injection of ferroglukin-75.