RU2404755C1 - Method for normalisation of spontaneous aggregation of erythrocytes in milk-fed piglets with anaemia - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: invention relates to the field of veterinary science. Method includes introduction of ferroglucin in amount of 75 mg (1 ml) intramuscularly three times with interval of 5 days, phosprenyl intramuscularly, three times in different syringes in different points simultaneously with preparation of iron, the first time - in amount of 0.10 ml/kg, the second time - in dose of 0.09 ml/kg, the third time - in dose of 0.08 ml/kg and gamavit in amount of 0.015 ml/kg intramuscularly once a day, for seven days, starting from the first injection of ferroglucin.

EFFECT: method makes it possible to avoid vascular complications in milk-fed piglets with anaemia, to optimise microcirculation and trophism in their tissues, to accelerate growth of animals, to make herd healthier, and to further receive healthy posterity and more meat produce from these animals.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for the correction of spontaneous aggregation of red blood cells (SAE) in piglets with milk anemia does not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as effective stimulants of the vital activity of a growing organism in phosprenyl, a drug based on polyprenol phosphates obtained from needles, and gamavit, a complex balanced means based on the placenta, containing sodium nucleate, a set of amino acids, vitamins, and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita. // Veterinary Medicine. - 2006. - No. 4. - p.13-15).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in milk-fed piglets with anemia in order to optimize their level of SAE immediately after treatment.

The aim of the invention is to improve the optimization of the level of SAE in piglets of dairy food with anemia.

The essence of the proposed method is that to optimize the level of SAE, milk piglets with anemia are prescribed ferroglukin 75 mg (1 ml) intramuscularly, three times with an interval of 5 days, fosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, first times 0.10 ml / kg, a second time at a dose of 0.09 ml / kg, a third time at a dose of 0.08 ml / kg and gamavit 0.015 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.

The method allows to optimize the level of SAE in dairy piglets with anemia immediately after treatment, translating the SAE to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to maintain a subsequent level of fibrinogenemia in an optimal condition, which will allow milk pigs with anemia to significantly reduce the risk of thrombotic complications.

The inventive method is as follows.

Red blood cell aggregation is assessed by the degree of ability of red blood cells to spontaneously aggregate (Pakhrova O.A., Grineva M.R., Ivanov K.S. Methodology and clinical significance of the study of rheological properties of blood // Bulletin of the Ivanovo Medical Academy. - 2008. - T 13, No. 1-2. - p. 89-98).

To assess the aggregation of red blood cells, blood from a vein is taken into sodium citrate at a ratio of 9: 1 and centrifuged for 10 min at 3000 rpm. In a 96-well plate, 2 wells of 0.2 ml of plasma of the subject are filled. All plasma and the white blood cell layer are removed from the tube. The red blood cells are resuspended in standard 1: 4 phosphate buffer, followed by centrifugation for 10 min at 3000 rpm, which allows them to be washed from plasma residues when removing the supernatant. After that, 0.02 ml of red blood cells is taken and resuspended in the first autologous plasma-filled well of a 96-well plate, which allows to obtain 10% hematocrit. Then, 0.02 ml of the contents are taken from this well with a clean dry pipette and placed in a second filled well, which makes it possible to obtain 1% hematocrit. After that, 1 grid in the Goryaev’s chamber is filled with the obtained erythrocyte suspension, kept for 3 min for the occurrence of spontaneous aggregation, and free erythrocytes (including 2 red blood cells together) and aggregates are counted, starting with 3 erythrocytes connected as “coin columns”) in 2 large squares of the camera (lens × 40, eyepiece × 10). The number of “coin columns” and the number of red blood cells involved in them are considered.

Based on the data obtained, the following indicators are calculated that evaluate the SAE:

1) Average unit size (CPA):

CPA = SEA / CA,

SEA - the sum of all red blood cells in the aggregates;

KA - the number of units;

2) Aggregation Index (PA):

PA = (CPA · KA + KSE) / (KA + KSE),

KSE - the number of free red blood cells;

3) Percentage of non-aggregated red blood cells (PNA):

PNA = KSE · 100 / (CPA · KA + KSE).

The standard values for CPA are 4-5, for PA 1.05-1.30, for PNA 80.0-92.0%. An increase in CPA above 5, PA above 1.30 and a decrease in PNA below 80.0% indicates an increase in SAE. In case of violation of at least one of these three indicators, sick animals need to be treated according to the claimed method.

When milk pigs with anemia are found to have an increase in SAE, they are prescribed therapy according to the claimed method - they are prescribed 75 mg ferroglukin (1 ml) intramuscularly, three times with an interval of 5 days, phosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation , the first time at a dose of 0.10 ml / kg, the second time at a dose of 0.09 ml / kg, the third time at a dose of 0.08 ml / kg and gamavit 0.015 ml / kg intramuscularly once a day, seven days, starting with the first injection ferroglukin.

Example. The number of hemoglobin in a milk pig with anemia No. 28 was 82.0 g / l, erythrocytes 4.16 × l0 ¹² / l, siderocytes 1.1% with a serum iron level of 12.6 μmol / l with an increase in the total iron binding capacity of serum blood up to 69.5 μmol / l. Blood was taken from a piglet in a plastic tube followed by a study of the aggregation activity of red blood cells (CPA = 5.9, PA = 1.79, PNA = 52.9%), which indicated an increase in SAE.

Piglet was prescribed ferroglukin 75 mg intramuscularly, three times with an interval of 5 days, phosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, the first time 0.10 ml / kg, the second time at a dose of 0.09 ml / kg, the third time at a dose of 0.08 ml / kg and gamavit 0.015 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.

The piglet was examined regularly during and after treatment. At the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 119.0 g / l, erythrocytes - to 4.95 × 10 ¹² / l, siderocytes to 8.7% with an increase in serum iron to 19.8 μmol / l and a decrease in the total iron binding capacity blood serum to 37.2 μmol / L with normalization of SAE (CPA = 4.9, PA = 1.16, PNA = 82.7%).

Using the proposed method for optimizing the level of SAE in biology and veterinary medicine will help to avoid vascular complications in dairy piglets with anemia, to optimize their microcirculation and trophic tissue, accelerating the growth of animals, improving the herd, which will allow to obtain more high-quality meat products from these animals in the future healthy offspring.

Claims (1)

A method of normalizing spontaneous aggregation of red blood cells in milk-fed piglets with anemia by applying ferroglukin 75 mg intramuscularly, three times with an interval of 5 days, fosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, the first time 0.10 ml / kg, a second time at a dose of 0.09 ml / kg, a third time at a dose of 0.08 ml / kg and hamavit 0.015 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.