RU2350321C1 - Method of normalisation of lipids content in membranes of thrombocytes at newborn pigs with anemia - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: way consists that ferrogluckine on 150 mg (2 ml) is prescribed intramuscularly, two times with an interval of 10 days, phosprenile intramuscularly, three times in different syringes in the different points, first time of 0.10 ml/kg simultaneously with an iron preparation, second time in a dose of 0.08 ml/kg in five days after the first ferrogluckine injection , third time in a dose of 0.05 ml/kg simultaneously with the second ferrogluckine injection. Thus in addition gamavit 0.014 ml/kg is injected intramuscularly once a day, eight days, since the first ferrogluckine injection.

EFFECT: method allows to optimise microcirculation and to lower risk of thrombotic complications.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method does not exist.

In the literature there are recommendations on the appointment of ferroglukin for the correction of anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. - M.: Agropromizdat, 1991. - 576 pp.) And gamavit for optimizing growth and improvement. metabolic processes (Deeva A.V., Salakhova Z.A., Lobova T.P. and others. Increasing the safety and productivity of piglets when using fosprenil and gamavit. // Veterinary medicine. - 2006. - No. 4. - p.13- fifteen).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn piglets with anemia to normalize the cholesterol / total phospholipids (cholesterol / phospholipids) gradient in the membranes of blood plates.

The aim of the invention is to increase the efficiency of correction of violations of the lipid composition of platelet membranes in newborn piglets with anemia.

The essence of the proposed method is that for the correction of the lipid composition of platelet membranes, newborn piglets with anemia are prescribed ferroglukin 150 mg (2 ml) intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0, 10 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and gamavit intramuscularly 0.014 ml / kg once a de s, eight days after the first injection ferroglyukina.

The method allows you to adjust the lipid composition of platelet membranes in newborn piglets with anemia 5 days after completion of the treatment, translating it to a level close to that for healthy animals. In animals treated in this way, the stable lipid composition of platelet membranes is subsequently observed to be stable, which will allow newborn piglets with anemia to optimize microcirculation and reduce the risk of thrombotic complications, increase weight gain, accelerate the growth and development of animals and reduce mortality.

The inventive method is as follows.

To assess the cholesterol / CFL gradient in platelets of the examined newborn piglet with anemia, the cholesterol level in the membranes of washed and resuspended blood plates is evaluated using an enzymatic colorimetric method using a set of Vital Diagnosticum and OFL for phosphorus content (Kolb V.G., Kamyshnikov BC Handbook Clinical Chemistry. Minsk, 1982. - 367 pp.) The standard values of cholesterol in the platelets of newborn calves are 0.62-0.73 micromol / 10 ⁹ tr., OFL 0.43-0.49 micromol / 10 ⁹ tr., gradient CS / OFL 1.26-1.69.

With anemia in newborn piglets, there is often an increase in the loading of cholesterol platelet membranes with a decrease in AF, which leads to an increase in the gradient of cholesterol / AF. When establishing this, a newborn piglet with anemia should be prescribed treatment with ferroglucin 150 mg intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, the first time 0.10 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and hamavit 0.014 ml / kg intramuscularly once a day, eight days starting from first injection of ferroglukin.

Example. In a newborn piglet No. 2 with anemia, a violation of erythropoiesis and signs of a decrease in the level of iron in its body (serum iron 13.0 μmol / l, siderocytes 1.4%, with an amount of hemoglobin of 80.0 g / l, erythrocytes - 3.90 × 10 ¹² / L.

The examination revealed a high content of cholesterol in the platelet membranes (1.06 μmol / 10 ⁹ tr.) And a low content of AFL (0.35 micromol / 10 ⁹ tr.), The gradient of cholesterol / AFL = 3.02. A piglet was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0.10 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.05 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.014 ml / kg intramuscularly once a day, eight days starting from the first injection of ferroglukin.

The pig was under constant clinical supervision with daily monitoring of the cholesterol / CFL gradient.

After 5 days at the end of the treatment, the pig achieved normalization of red blood - serum iron 20.5 μmol / L, siderocytes 8.2%, with an average hemoglobin of 117.0 g / L, erythrocytes - 5.02 × 10 ¹² / l with a decrease in the total iron-binding ability of blood serum to 38.9 μmol / l, normalization of the content of cholesterol and OFL in platelet membranes was achieved (0.66 μmol / 10 ⁹ tr., 0.48 micromol / 10 ⁹ tr.) with optimization gradient of cholesterol / AFL = 1.37, which provided a reduced risk of platelet activation and the development of thrombosis in the future.

Using the proposed method for correcting the lipid composition of platelet membranes in newborn piglets with anemia in biology and veterinary medicine will help to avoid many vascular complications and optimize microcirculation and tissue trophism in animals, improve livestock numbers, reduce mortality, accelerate piglet growth and growth, and increase weight gain.

Claims (1)

A method of normalizing the lipid content in platelet membranes in newborn piglets with anemia, including the use of 150 mg ferroglukin intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, three times in different syringes at different points, the first time 0.10 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.08 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.05 ml / kg simultaneously with a second injection of ferroglukin and gamavit 0.014 ml / kg intramuscularly once a day, eight days starting from ditch injection of ferroglukin.