RU2494734C1 - Method for normalising spontaneous erythrocyte aggregation in newborn piglets suffered acute hypoxia at birth - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science, and may be used for normalising spontaneous erythrocyte aggregation (SEA) in newborn piglets suffered acute hypoxia at birth. That is ensured by prescribing crezacin 4 mg/kg a day of fed-on plan in the morning for five days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for five days in the newborn piglets suffered acute hypoxia at birth.

EFFECT: method enables normalising SEA in the newborn piglets, normalising weight gain, reducing death loss and improving the quality of meat products.

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Description

The invention relates to biology and veterinary medicine, namely to hematology.

Analogues of the claimed method of normalizing the spontaneous aggregation of red blood cells (SAE) in newborn piglets who underwent acute hypoxia at birth do not exist. There is information in the literature on the high biological activity of crezacin, a derivative of aroxyalkylcarboxylic acids, capable of stimulating various systems of living organisms, increasing their survival under adverse conditions (Baimatov V.N., Latypov M.M. . - No. 11. - S.49-51) and gamavita - a complex balanced means based on the placenta containing sodium nucleinate, a set of amino acids, vitamins and salts, which has a high organoprotective and anabolic effect (White Usova R.V. Use of gamavit to increase the efficiency of pig reproduction // Veterinary Medicine - 2006 - No. 10. - S.11-12). However, in the literature there are no indications of their effect on SAE and, all the more, on the possibility of normalization with their help of SAE in newborn piglets that underwent acute hypoxia at birth.

The aim of the invention is the normalization of SAE in newborn piglets who underwent acute hypoxia at birth.

The essence of the proposed method lies in the fact that to normalize the SAE, newborn piglets who underwent acute hypoxia at birth are prescribed krezacin 4 mg / kg per day, which is included in the watering schedule for five days in the morning, and at the same time, gamavit 0.03 mg / kg intramuscularly once a day, in the morning, for five days.

The method allows to stably normalize SAE in newborn piglets who underwent acute hypoxia at birth, 5 days after the end of treatment. Subsequently, in these piglets, the SAE is stably at a normal level, which allows to significantly improve the number of pigs, normalize weight gain, reduce their pathological burden, reduce mortality and improve the quality of meat products received from them in the future.

The inventive method is as follows.

Red blood cell aggregation is assessed by the degree of ability of red blood cells to spontaneously aggregate (Pakhrova O.A., Grineva MR, Ivanov K.S. Methodology and clinical significance of the study of rheological properties of blood // Bulletin of the Ivanovo Medical Academy. - 2008. - T13, No. 1 -2. - p. 89-98.).

To assess the aggregation of red blood cells, blood from a vein is taken into sodium citrate at a ratio of 9: 1 and centrifuged for 10 minutes at 3000 rpm. In a 96 well plate, 2 wells of 0.2 ml of plasma of the subject are filled. All plasma and the white blood cell layer are removed from the tube. The red blood cells are resuspended in standard 1: 4 phosphate buffer, followed by centrifugation for 10 min at 3000 rpm./min, which allows them to be washed from plasma residues when removing the supernatant. After that, 0.02 ml of red blood cells is taken and resuspended in the first autologous plasma-filled well of a 96 well plate, which allows to obtain 10% hematocrit. Then, 0.02 ml of the contents are taken from this well with a clean dry pipette and placed in a second filled well, which allows to obtain 1% hematocrit. After that, 1 grid in the Goryaev’s chamber is filled with the obtained erythrocyte suspension, incubated for 3 minutes. for the occurrence of spontaneous aggregation, they count free red blood cells (including 2 red blood cells together) and aggregates starting with 3 red blood cells connected in the form of “coin columns”) in 2 large squares of the camera (lens × 40, eyepiece × 10 ) The number of “coin columns” and the number of red blood cells involved in them are considered.

Based on the data obtained, the following indicators are calculated that evaluate the SAE:

1) Average unit size (CPA):

CPA = SEA / CA,

SEA - the sum of all red blood cells in the aggregates;

KA - the number of units,

2) Aggregation Index (PA):

PA = (CPA · KA + KSE) / (KA + KSE),

KSE - the number of free red blood cells,

3) Percentage of non-aggregated red blood cells (PNA):

PNA = KSE · 100 / (CPA · KA + KSE).

The standard values for CPA are 4-5, for PA 1.05-1.30, for PNA 80.0-92.0%. An increase in CPA above 5, PA above 1.30 and a decrease in PNA below 80.0% indicates an increase in SAE. In case of violation of at least one of these three indicators, sick animals must be treated according to the claimed method, including crezacin 4 mg / kg per day in a 5-day rehydration regimen and at the same time gamavit 0.03 ml / kg intramuscularly once a day for five days.

The use of the claimed complex normalizes SAE in newborn piglets who underwent acute hypoxia at birth, 5 days after the end of treatment. This is the basis for improving the herd, reducing the mortality rate and guaranteeing the high quality of meat products obtained from piglets treated using the claimed method.

Example 1. In a newborn piglet No. 34, who underwent acute hypoxia at birth, an increase in SAE was detected (CPA = 5.9, PA = 1.82, PNA = 64.8%). Piglet was prescribed simultaneously krezacin 4 mg / kg per day in the scheme of drinking in the morning for 5 days and gamavit 0.03 ml / kg intramuscularly once a day, in the morning for five days.

5 days after the end of treatment, the piglet registered a complete normalization of SAE (CPA = 4.1, PA = 1.21, PNA = 86.1%). Subsequently, her performance in the piglet did not experience negative dynamics.

Example 2. In a newborn piglet No. 38, who underwent acute hypoxia at birth, an increase in SAE was detected (CPA = 5.4, PA = 1.72, PNA = 67.4%). Piglets were simultaneously prescribed krezacin 4 mg / kg per day in the morning vaping schedule for 5 days and gamavit 0.03 ml / kg intramuscularly once a day, in the morning for 5 days.

5 days after the end of treatment, the piglet achieved full normalization of the level of SAE (CPA = 4.3, PA = 1.24, PNA = 83.8%). During subsequent observation, the considered indicators for this pig did not experience negative dynamics.

Example 3. In piglet No. 40, who underwent acute hypoxia at birth, an increase in SAE was detected (CPA = 5.8, PA = 1.77, PNA = 68.6%). The piglet was prescribed krezacin 4 mg / kg per day in the morning vaping schedule for 5 days and gamavit 0.03 ml / kg intramuscularly once a day, in the morning for 5 days.

5 days after the end of treatment, the piglet achieved full normalization of SAE (CPA = 4.2, PA = 1.28, PNA = 88.0%), which subsequently did not experience negative dynamics.

The application of the claimed method has been successfully tested on 128 newborn piglets who underwent acute hypoxia at birth, with the achievement of full normalization of SAE in all cases.

Claims (1)

A method of normalizing the spontaneous aggregation of red blood cells in newborn piglets who underwent acute hypoxia at birth, including the simultaneous use of crezacin 4 mg / kg per day in the scheme of drinking in the morning for five days and gamavita 0.03 ml / kg intramuscularly once a day, in the morning for five days.