RU2488387C1 - Method for normalising spontaneous erythrocyte aggregation in suckling piglets with arthritis - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: invention refers to veterinary science, and may be used for normalising spontaneous erythrocyte aggregation in suckling piglets with arthritis. That is ensured by prescribing crezacin 4 mg/kg a day of fed-on plan in the morning for five days combined with gamavit 0.03 mg/kg intramuscularly once a day in the morning for five days in the suckling piglets with arthritis.

EFFECT: method enables normalising spontaneous erythrocyte aggregation in piglets, reducing death loss, normalising weight gain, and improving the quality of meat products.

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Description

The invention relates to biology and veterinary medicine, namely to hematology.

Analogues of the claimed method of normalizing the spontaneous aggregation of red blood cells (SAE) in piglets of milk food with arthritis does not exist.

The literature contains information on the high biological activity of krezacin, a derivative of aroxyalkylcarboxylic acids, which can stimulate various systems of living organisms, increasing their survival under adverse conditions (Baimatov V.N., Latypov M.M. Effect of krezacin on the state of birds // Veterinary Medicine. - 2006 . - No. 11 - S.49-51) and gamavita - a comprehensive balanced means based on the placenta containing sodium nucleinate, a set of amino acids, vitamins and salts, which has a high organoprotective and anabolic effect (White owl RV Application gamavita to improve the efficiency and reproduction of pigs // Veterinary Medicine -. 2006. - №10 -. S.11-12). However, in the literature there are no indications of their effect on SAE and, all the more, on the possibility of normalizing SAE in milk piglets with arthritis with their help.

The aim of the invention is the normalization of SAE in piglets of dairy food with arthritis.

The essence of the proposed method lies in the fact that to normalize the SAE, milk pigs with arthritis are prescribed crezacin 4 mg / kg per day, included in the watering schedule for five days in the morning, and at the same time gamavit 0.03 mg / kg intramuscularly once a day , in the morning, for five days.

The method allows to stably normalize SAE in piglets of milk food with arthritis 5 days after the end of treatment. Subsequently, in these piglets, the SAE is stably at a normal level, which allows to significantly improve the number of pigs, normalize weight gain, reduce their pathological burden, reduce mortality and improve the quality of meat products received from them in the future.

The inventive method is as follows.

Red blood cell aggregation is assessed by the degree of ability of red blood cells to spontaneously aggregate (Pakhrova O.A., Grineva MR, Ivanov K.S. Methodology and clinical significance of the study of rheological properties of blood // Bulletin of the Ivanovo Medical Academy. - 2008. - T13, No. 1 -2. - p. 89-98.).

To assess the aggregation of red blood cells, blood from a vein is taken into sodium citrate at a ratio of 9: 1 and centrifuged for 10 min at 3000 rpm. In a 96 well plate, 2 wells of 0.2 ml of plasma of the subject are filled. All plasma and the white blood cell layer are removed from the tube. The red blood cells are resuspended in standard 1: 4 phosphate buffer, followed by centrifugation for 10 min at 3000 rpm, which allows them to be washed from plasma residues while removing the supernatant. After that, 0.02 ml of red blood cells is taken and resuspended in the first autologous plasma-filled well of a 96 well plate, which allows to obtain 10% hematocrit. Then, 0.02 ml of the contents are taken from this well with a clean dry pipette and placed in a second filled well, which allows to obtain 1% hematocrit. After that, 1 grid in the Goryaev’s chamber is filled with the obtained erythrocyte suspension, kept for 3 min for the occurrence of spontaneous aggregation, and free erythrocytes (including 2 red blood cells together) and aggregates are counted, starting with 3 erythrocytes connected in the form of “coin columns”) in 2 large squares of the camera (lens × 40, eyepiece × 10). The number of “coin columns” and the number of red blood cells involved in them are considered.

Based on the data obtained, the following indicators are calculated that evaluate the SAE:

1) Average Unit Size (CPA):

CPA = SEA / CA

SEA - the sum of all red blood cells in the aggregates;

KA - the number of units

2) Aggregation Index (PA):

PA = (CPA · KA + KSE) / (KA + KSE)

KSE - the number of free red blood cells

3) Percentage of non-aggregated red blood cells (PNA):

PNA = KSE · 100 / (CPA · KA + KSE)

The normative values for CPA are 4-5, for PA 1.05-1.30, for PNA 80.0-92.0%. An increase in CPA above 5, PA above 1.30 and a decrease in PNA below 80.0% indicates an increase in SAE. In case of violation of at least one of these three indicators, sick animals must be treated according to the claimed method, including crezacin 4 mg / kg per day in a 5-day rehydration regimen and at the same time gamavit 0.03 ml / kg intramuscularly once a day for five days.

The use of the claimed complex completely normalizes SAE, eliminating the signs of arthritis, in piglets of milk feeding with arthritis 5 days after the end of treatment. This is the basis for improving the herd, reducing the mortality rate and guaranteeing the high quality of meat products obtained from piglets treated with it.

Example 1. In a piglet dairy food No. 54 with arthritis, an increase in SAE was detected (CPA = 5.6, PA = 1.80, PNA = 66.2%). Piglet was prescribed simultaneously krezacin 4 mg / kg per day in the scheme of drinking in the morning for 5 days and gamavit 0.03 ml / kg intramuscularly once a day, in the morning for five days.

5 days after the end of the treatment, the signs of arthritis and complete normalization of SAE were recorded in the piglet (CPA = 4.3, PA = 1.24, PNA = 85.7%). Subsequently, the achieved SAE values in the piglet did not experience negative dynamics.

Example 2. In a piglet dairy food No. 56 with arthritis, an increase in SAE was detected (CPA = 5.3, PA = 1.67, PNA = 62.9%). Piglets were simultaneously prescribed krezacin 4 mg / kg per day in the morning vaping schedule for 5 days and gamavit 0.03 ml / kg intramuscularly once a day, in the morning for 5 days.

5 days after the end of treatment, the pig achieved the elimination of arthritis and a complete normalization of the level of SAE (CPA = 4.6, PA = 1.26, PNA = 83.8%). Upon subsequent observation, the SAE values for this piglet did not experience negative dynamics.

Example 3. In a piglet dairy food No. 59 with arthritis, an increase in SAE was detected (CPA = 5.8, PA = 1.83, PNA = 68.9%). The piglet was prescribed krezacin 4 mg / kg per day in the morning vaping schedule for 5 days and gamavit 0.03 ml / kg intramuscularly once a day, in the morning for 5 days.

5 days after the end of treatment, the pig achieved arthritis elimination and full normalization of SAE (CPA = 4.4, PA = 1.24, PNA = 87.2%), which subsequently did not experience negative dynamics.

The application of the claimed method has been successfully tested on 132 piglets of milk nutrition with arthritis with the achievement of full normalization of SAE in all cases.

Claims (1)

A method for normalizing the spontaneous aggregation of red blood cells in milk-fed piglets with arthritis, including the simultaneous use of krezacin 4 mg / kg per day in the morning vodication regimen for five days and gamavita 0.03 ml / kg intramuscularly once a day, in the morning for five days.