RU2350320C1 - Method of fibrinogenemia level optimisation at newborn pigs with anemia - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: way consists that for optimisation of fibrinogenemia level ferroglukine on 75 mg (2 ml) is prescribe intramusculary, three times with an interval of 6 days, phosprenil intramusculary, three times in different syringes in different points simultaneously with an iron preparation, first time of 0.10 ml/kg, second time in a dose of 0.08 ml/kg, third time in a dose of 0.07 ml/kg. Thus in addition gamavit 0.016 ml/kg is entered intramusculary once a day, seven days, since the first ferroglukine injection.

EFFECT: method allows to avoid vascular complications at newborn pigs with an anaemia, to optimise at them microcirculation and a trophicity in tissues.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for the correction of hyperfibrinogenemia in newborn piglets with anemia do not exist.

In the literature there is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M.: Agropromizdat, 1991. - 576 p.).

In modern veterinary medicine, phosprenyl is used as effective stimulants of the vital activity of a growing organism in phosprenyl, a drug based on polyprenol phosphates obtained from needles, and gamavit, a complex balanced means based on the placenta, containing sodium nucleate, a set of amino acids, vitamins, and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita. // Veterinary Medicine. - 2006. - No. 4. - p.13-15).

However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn piglets with anemia in order to optimize their fibrinogenemia levels immediately after treatment.

The aim of the invention is to improve the optimization of the level of fibrinogenemia in newborn piglets with anemia.

The essence of the proposed method is that to optimize the level of fibrinogenemia, newborn piglets with anemia are prescribed ferroglukin 75 mg (2 ml) intramuscularly, three times with an interval of 6 days, fosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, for the first time 0.10 ml / kg, a second time at a dose of 0.08 ml / kg, a third time at a dose of 0.07 ml / kg and gamavit 0.016 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.

The method allows to optimize the level of fibrinogenemia in newborn piglets with anemia immediately after treatment, translating fibrinogenemia to a level close to that for healthy piglets. Subject to the recommendations of the proposed method, it is possible to maintain a subsequent level of fibrinogenemia in optimal condition, which will allow newborn piglets with anemia to significantly reduce the risk of thrombotic complications.

The inventive method is as follows.

Hyperfibrinogenemia is established in the examined piglet by the following method (Barkagan Z.S., Momot A.P. Fundamentals of the diagnosis of hemostatic disorders. M, 1999. - p. 84-86).

The following reagents are used: 1. Phosphate buffer, pH 7.0 (0.06 M) of the following composition: 190 ml 0.5 M KN ₂ PO ₄ (68.045 g per 1 l) and 110 ml 0.5 N sodium hydroxide. For operation, 20 ml of this basic buffer solution is brought to a volume of 100 ml with distilled water. 2. 0.15 M sodium chloride solution. 3. 0.04 M solution of monoiodoacetic acid in 0.06 M phosphate buffer (40 mg of monoiodoacetic acid in 5 ml of buffer). 4. Pig thrombin, 50 NIH units / ml. 5. 1.5% solution of acetic acid.

Applied equipment. Spectrophotometer with optical density measurement in the ultraviolet zone.

The material for the study is a citrate platelet-poor plasma.

1.6 ml of 0.06 M phosphate buffer was added to a test tube with 0.2 ml of plasma and placed in a water bath (37 ° C). After a few minutes, 0.1 ml of a 0.04 M solution of monoiodoacetic acid was added to the mixture and after 3 minutes 0.1 ml of a thrombin solution was added. After adding each component, the contents of the tube are thoroughly mixed with a glass rod (with a rough surface at the end), leaving it in the tube. The resulting mixture is incubated at 37 ° C for at least 15 minutes (longer incubation is allowed). The resulting fibrin clot is removed by winding onto a stick, and squeezed against the wall of the tube. The clot is washed twice in 50 ml of a 0.15 M sodium chloride solution. Then washed once in chilled distilled water. Water from the surface of the clot is additionally removed with filter paper. The resulting clot is dissolved with stirring in 5 ml of 1.5% acetic acid at room temperature. Dissolution complete in 5 minutes. The protein content in the solution is determined spectrophotometrically by measuring the absorbance at 280 and 320 nm. The extinction is stable, the measurement time can be arbitrarily selected.

The concentration of fibrinogen (X) in the test plasma sample is calculated by the formula:

,

,

where E (280-320) is the difference between the values of extinction at 280 and 320 nm, thereby achieving the necessary correction for turbidity; 255 — coefficient for expressing the amount of fibrinogen in g / l in an analysis performed with 0.2 ml of plasma; 15,067 is the extinction coefficient for fibrinogen in an acidic medium at a wavelength of 280 nm (E 1% 1 cm).

Normally, the fibrinogen content determined by this method is 2.0-4.0 g / l.

When newborn piglets with hyperfibrinogenemia anemia are found, they are prescribed therapy according to the claimed method - they are prescribed ferroglukin 75 mg (2 ml) intramuscularly, three times with an interval of 6 days, phosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, for the first time 0.10 ml / kg, a second time at a dose of 0.08 ml / kg, a third time at a dose of 0.07 ml / kg and gamavit 0.016 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.

Example. In a newborn piglet with anemia No. 22, the amount of hemoglobin was 83.0 g / l, red blood cells 4.11 · 10 ¹² / l, siderocytes 1.2% with a serum iron level of 12.2 μmol / l with an increase in the total iron binding capacity of blood serum up to 69.6 μmol / l, hyperfibrinogenemia level (7 g / l).

A piglet was prescribed ferroglukin 75 mg intramuscularly, three times with an interval of 6 days, phosprenyl intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, the first time 0.10 ml / kg, the second time at a dose of 0.08 ml / kg, the third time at a dose of 0.07 ml / kg and gamavit 0.016 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.

The piglet was examined regularly during and after treatment. At the end of treatment, the red blood returned to normal in the piglet. So, the amount of hemoglobin in it increased to 118.5 g / l, erythrocytes - to 4.99 · 10 ¹² / l, siderocytes to 8.6% with an increase in serum iron to 19.9 μmol / l and a decrease in the total iron binding capacity blood serum up to 37.9 μmol / l, hyperfibrinogenemia was not detected (2 g / l).

Using the proposed method for optimizing the level of fibrinogenemia in biology and veterinary medicine will help to avoid vascular complications in newborn piglets with anemia, to optimize their microcirculation and trophic tissue, accelerating the growth of animals, improving the herd, which will allow to receive more high-quality meat products and subsequently healthy animals progeny.

Claims (1)

A method for optimizing the level of fibrinogenemia in newborn piglets with anemia by applying ferroglukin 75 mg intramuscularly, three times with an interval of 6 days, fosprenil intramuscularly, three times in different syringes at different points simultaneously with the iron preparation, the first time 0.10 ml / kg , a second time at a dose of 0.08 ml / kg, a third time at a dose of 0.07 ml / kg and gamavit 0.016 ml / kg intramuscularly once a day, seven days, starting with the first injection of ferroglukin.