RU2315487C1 - Biologically active product from brown algae, biologically active food supplement, soft drink, perfume and cosmetic agent - Google Patents

Abstract

FIELD: food processing and cosmetic industries.

SUBSTANCE: claimed biologically active product represents polysaccharide composition obtained from Fucus evanescens algae and containing 60-80 % of fucoidane and 20-40 % of polymannuronic acid. Said product is additional source of immune active polysaccarides and soluble diet fibers and is useful as raw materials in production of biologically active food supplements and healthy soft drinks. Moreover it may be used as raw materials for perfume and cosmetic agent with immunomodulating action.

EFFECT: products with active biological action and prolonged storage time.

5 cl, 6 ex, 5 tbl

Description

The group of inventions relates to the food and cosmetic industry and relates to biologically active products from brown algae, and also relates to biologically active food additives, drinks and cosmetics based on them.

Seaweed is a valuable and stabilizing food for the human body. Around the world, there is a growing interest in using them in food. However, seaweed can not only be directly consumed, but also obtained from them various biologically active substances, as the basis of biologically active food additives and functional foods.

Known biologically active products based on seaweed contain either the algae themselves, or extracts or concentrates from them.

Known natural product based on seaweed (seaweed or fucus, or a mixture thereof), providing a physiological norm in micro and macro elements, a complex of vitamins, iodine, essential and essential amino acids [RU 2122812 C1, 10.12.1998]. The biologically active additive "Zdravitsa-L", which has a healing effect in various diseases and pathological conditions, contains food kelp and wheat bran [RU 2170585 C1, 07.20.2001]. The product "Lamifaren" with an adsorbing effect, which is a specially treated algae containing free alginate in the form of calcium and sodium salts, macro- and microelements [RU 2230464 C1, 20.06.2004].

A biologically active food supplement is known to compensate for the lack of iodine in the body, which contains kelp extract [RU 2164144 C1, 03/20/2001], as well as a dietary supplement, which contains a concentrate as a micro- and macroelement, adaptogen and / or detoxifier laminaria [RU 2142240 C1, 12/10/1999]. Known dietary supplement containing a fucus concentrate [RU 2165720 C2, 04/27/2001]. It is recommended to use it in various food products, both with the aim of replenishing the missing macro and microelements, vitamins, and as an adaptogen and / or detoxifier.

Known biologically active additive containing a water-soluble concentrate (VK) from brown algae - kelp and fucus vesicular Fucus vesiculosis, which has adaptogenic and detoxifying effects [RU 2152737 C1, 07.20.2000]. VK contains proteins (including amino acids), sugars, micro and macro elements, including iodine. It can be used in the production of various food products, including soft drinks, as well as in the manufacture of perfumes and cosmetics.

However, the known products, biologically active additives from brown algae have inherent disadvantages in that the uncertainty of their composition makes it difficult to standardize these products, both in composition and biological effect, and the lipids contained in the concentrates oxidize over time, which leads to their reduction quality and shelf life. In addition, dietary supplements based on an aqueous concentrate of brown algae can have a side effect when used for people with thyroid diseases (hyperfunction of the thyroid gland), as well as with diseases in which iodine preparations are contraindicated (pulmonary tuberculosis, nephritis, nephrosis, hemorrhagic diathesis, furunculosis )

Global trends in the development of the production of dietary supplements unequivocally lead to its complication, increased processing depth, degree of extraction, purification of active ingredients and the creation of parapharmaceutical products (dietary supplements, medical cosmetics) and food products on their basis.

Japan, one of the first countries in the world, began the development and patenting of products based on individual polysaccharides, in particular fucoidan [priority applications JP 19960171666, 06/12/1996; JP 19960318598, 11/15/1996; patent US 6573250, 06/03/2003 and other patents-analogues].

Food and drinks in accordance with this invention contain, as fucoidan, the so-called fucoidan-U and / or fucoidan-P, which have apoptotic activity.

Takara Bio Joint Stock Company and its biotechnology department started with the launch of the Apoidan-V® drink containing fucoidan in 1996, and then they began selling a wide variety of healthy food products. The raw material was Kjellmaniella crassifolia, collected in the southwest of the Hokkaido coast, the most mucous and with the highest fucoidan content of all available brown algae. It contains 5% fucoidan of dry weight, which is two times more than in Laminaria japonica [Savago and Kato "Fucoidan as a functional food product. Structure and biological activity. Translation from Japanese of an article from the journal Japan. J.Phycol. (Sorui) "51, 2003, p. 10].

Purified fucoidan from K. crassifolia was freeze-dried. A dried product was obtained in the form of fibers, which was ground to increase its solubility. In 1997, it was marketed as a food raw material and named "Takarakombifucoidan®". The product contains sulfated fucoidan SFGM (fucoglucuronomannan), which causes cancer cell apoptosis. This product is a dietary fiber consisting of fucoidan, almost pure in its mass, without any additives. In order for this product to be suitable as a food raw material, a specific algal odor is removed from it and only a pleasant aroma of brown algae is left. Given this, the proposed product can be easily used for a wide variety of food products. It is approved for use by the American FDA and is sold worldwide as a raw material.

Foreign companies, mainly Japanese, Korean companies are constantly developing more and more new products containing fucoidan, products of its enzymatic hydrolysis and other biologically active polysaccharides [JP 10165114, 06.23.1998, JP 2002223727, 08/13/2002; JP 2002306131. 10/22/2002; JP 2003135028, May 13, 2003; JP 2004024054, Jan 29, 2004; KR 2001019225, 03/15/2001].

These firms distribute their fucoidan-containing products to the US and European markets. In the Russian market, there are practically no similar products containing individual polysaccharides from algae obtained using modern methods of isolation and highly effective purification.

Therefore, the task was to create a new product based on biologically active polysaccharides of brown algae growing in the Far Eastern seas of Russia, and the task was to create a biologically active food supplement, drinks and cosmetics based on it.

As a result of the solution of the problem, a biologically active product was created containing polysaccharides of brown algae, which according to the invention as polysaccharides of brown algae contains a polysaccharide composition obtained from alga Fucus evanescens, consisting of fucoidan in an amount of 60-80% and polymannuronic acid in an amount of 20- 40%, and the target product is used as a raw material for the production of biologically active food additives, drinks and cosmetics.

A polysaccharide composition from Fucus evanescens, consisting of fucoidan in an amount of 60-80% and polymannuronic acid in an amount of 20-40% in the patent and other scientific literature is not found.

The applicant proposes to use brown algae Fucus evanescens (disappearing fucus) to obtain a new product, which has not been previously used by anyone to obtain a polysaccharide composition in order to use it as a raw material for biologically active food additives, drinks and cosmetic products.

The use of this type of algae to obtain a new product has an advantage over other types of algae due to the high content of fucose-containing polysaccharides in it. So the content of fucoidan in it is 10-15% of the weight of dry algae, which exceeds this indicator in Kjellmaniella crassifolia algae by 2-3 times.

The alga Fucus evanescens has an extensive distribution area, grows in shallow water, is easily accessible and is found along the entire coast of the Kuril Islands and Kamchatka, as well as in the Primorsky Territory and Sakhalin [Shuntov V.P. Biology of the Far Eastern Seas of Russia. Vladivostok: TINRO Center. - 2001. - 580 p.].

The method of obtaining the inventive product from Fucus evanescens is simple because it involves only extraction and purification of the product by ultrafiltration, in contrast to the method for producing the known product "Takarakombifucoidan®", which is a mixture of low molecular weight fucoidans, however, these fucoidans or their mixture were obtained by laborious, expensive technology , including the enzymatic treatment of high molecular weight polysaccharides with alginate lyase [Savago and Kato "Japan. J. Phycol. (Sorui)" 51, 2003, p. 3] or endo-fucoidan-lyase [US 6573250, 03.06.2003].

The method of obtaining the claimed composition does not include enzymatic treatment, but despite this, the polysaccharides that make up the composition have a low molecular weight (not more than 80 kDa), and therefore, the composition has very good solubility in water. This also consists of the advantage of the claimed product in comparison with the known. Good solubility is very important when using the polysaccharide composition as a raw material for the production of healthy foods and cosmetics.

The product from Fucus evanescens was named "Fucolam-S" (the applicant has filed an application for registration of a trademark). It is an additional source of immunoactive polysaccharides and soluble dietary fiber that increase the nonspecific resistance of the body, and can be used as a raw material for dietary supplement for the prevention of painful conditions of the body caused by general physical weakening of the body, decreased immunity, to improve the condition of vital systems and human organs.

Animal studies were performed at the Research Institute of Epidemiology and Microbiology SB RAMS (Vladivostok). The experiment investigated the acute toxicity of fucolam-C, the effect of this product on non-specific resistance indices, the survival of mice with endotoxic shock, specific reactions of the humoral and cellular type, in vitro cytokine production, and the effect on the hemostasis system. It was found that fucolam-C is not toxic in the studied dose range (from 0.5 to 250 mg / kg), has a stimulating effect on factors of nonspecific resistance of the body and on a specific immune response by inducing the expression of a number of regulatory cytokines, and contributes to increased survival and longevity animals with endotoxemia, has anti-inflammatory activity.

Studies have shown that fucolam-C also has a beneficial effect on liver function, and this is apparently due to the polymannuronic acid present in the polysaccharide composition. It is known that alginate enriched with polymannuronic acid protects the liver more effectively from obesity than alginate enriched with guluronic acid [J. Biology of the sea. 2001, volume 27, No. 3, p. 157].

Thus, the important advantages of fucolam-C are the economical and environmentally friendly production technology of the product, its low toxicity to the body, good solubility, a large degree of dosage freedom, and a wide range of biological activity.

Fucoidans are known to have anticoagulant activity in vitro and in vivo, comparable to that of heparin. Studies of the anticoagulant effect of fucolam-C revealed another advantage: when using fucolam-C per os at a dose of 100 mg / kg, it does not have an anticoagulant effect. Therefore, it has no contraindications for use by people with reduced blood coagulation. In addition, the inventive product does not contain iodine, and therefore can be recommended for people with thyroid diseases and with diseases in which iodine preparations are contraindicated.

The invention expands the possibilities of using products based on brown algae polysaccharides, and also expands the range of such products. Moreover, in comparison with algal extracts and concentrates widely represented on the Russian market, the claimed product has an advantage, because It is more standardized in composition and, therefore, has a directed biological effect. The product does not contain substances of lipid nature, and therefore the necessary quality parameters are stored for a longer period. The shelf life of fucolam-C is 3 years, the shelf life of algal concentrates, extracts containing substances of lipid nature is not more than 1 year.

Fucolam-C is prepared as follows.

Raw or thawed seaweed Fucus evanescens is previously degreased. Ethyl alcohol 80-96% or acetone or nephraz in the ratio of raw materials: absolute alcohol 1: 1 is used as an extractant. Algae is dried from residual solvent. To extract the polysaccharide complex, the algae is extracted twice with an aqueous solution of hydrochloric acid at a ratio of raw materials: extractant 1:20 with stirring for 3 hours. The extraction is carried out at a pH of not higher than 6 and a temperature of 50-100 ° C. The finished extract is poured into a container, while filtering. Precipitation centrifuges or separators are recommended for purification of the extract. The spent seaweed is squeezed out on a press filter.

The extract is passed through a sterilizing microfiltration unit with membranes and concentrated on an ultrafiltration unit with separation membranes 3-4 times of the original volume. The concentrate is neutralized to pH 5.5-6.5 with sodium bicarbonate solution. To remove low molecular weight impurities, an equal volume of distilled water is added to the obtained concentrate and again concentrated to the original volume. The washing process is repeated 2-3 times. The washed concentrate is evaporated on a vacuum evaporator 4-5 times from the initial volume of the concentrate.

The target product is dried in a freeze dryer or precipitated with ethyl alcohol at a concentration of 80-96% in the presence of calcium chloride or magnesium chloride or sodium chloride. The precipitate is squeezed out on a press filter, dehydrated, squeezed out again and dried in an explosion-proof vacuum drum dryer.

Fucolam-C is made in the form of a powder. Grinding is carried out on a hammer-type grinder with a set of sieves. The crushed powder is sieved through a sieve with a mesh size of 1 mm and Packed in bags of film materials weighing from 0.5 to 5.0 kg Bags must be sealed to ensure tight seams.

To determine the qualitative and quantitative composition of fucolam-C, they are analyzed by the Dishe method (fucoidan) and the carbosol method (polymannuronic acid).

The inventive product was examined at the Institute of Nutrition RAMS and is currently registered with the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being as a raw material for the production of biologically active additives for the food industry and cosmetology.

The applicant uses this product to obtain Fukolam dietary supplement, which contains fucolam-C and calcium alginate in the following ratio of components, wt.%:

Fukolam-S - 20.

Calcium Alginate - 80

Calcium alginate is used as a filler. At the same time, it serves as an additional source of soluble fiber.

To obtain the claimed dietary supplement Fucolam-C and calcium alginate in the amounts prescribed by the formulation, are loaded into a ball mixer and mixed until the components are evenly distributed (about 2 hours). At the end of the mixing process, the mass is discharged from the mixer into bags or containers and with an accompanying label sent for encapsulation. The finished product sent for packaging and packaging must be pre-checked for compliance of its indicators with the requirements of the standard. Encapsulation is carried out on specialized equipment. The mass of the contents of the capsule is 0.5 g.

Safety indicators of dietary supplements "Fukolam" do not exceed the permissible levels regulated by SanPiN 2.3.2.1078-01 for dietary supplements based on algae.

Studies have shown that the recommended dose of fucolam-C for humans is 100 mg per day. Method of application: Adults take 1 capsule once a day during or after meals for 1 month. Shelf life: 3 years from the date of manufacture.

Fukolam dietary supplement is registered in the Federal dietary supplement dietary supplement No. 77.99.23.3.U.739.1.06 of January 30, 2006, is recommended as an additional source of polysaccharides and soluble dietary fiber, and increases the nonspecific resistance of the body.

The technical result is the expansion of the possibility of using products based on brown algae polysaccharides, namely, the claimed dietary supplement for food can be consumed by people with reduced blood coagulation, people with thyroid diseases and with diseases in which dietary supplements containing iodine are contraindicated. The inventive dietary supplement has all the advantages that are inherent in the substance - fucolam-S. It has a standard composition and directed biological effect. The invention provides an expansion of the range of products based on individual brown algae polysaccharides.

Known non-alcoholic tonic drink "Vita-marina" containing sugar, natural honey, apple pectin, citrus pectin, zosterin (polysaccharide from sea grass zostera), citric acid, viburnum juice, rosehip extract, sparkling or still water [RU 2125816 C1, 10.02 .1999]. The drink has therapeutic and prophylactic properties.

Closest to the claimed beverage according to the main functional component contained in it is the drink "Apoidan-V®", created on the basis of fucoidan U and / or fucoidan-F, which is obtained in a laborious and expensive way from the alga Kjellmaniella crassifolia, growing only off the coast of Japan [ Savago and Kato "Japan. J. Phycol. (Sorui)" 51, 2003, p. 11], [US 6573250. 03.06.2003].

As a result of solving the task, the applicant created a composition for a drink containing a biologically active product - fucolam-C, obtained in a simple way from affordable, environmentally friendly alga Fucus evanescens.

The composition of the ingredients for a soft drink is characterized by the fact that it contains fucolam-C, an infusion of rose hips and hawthorn fruits, a tincture of the roots of Rhodiola rosea, sorbic acid, succinic acid, honey and water in the following ratio of ingredients, kg / 100 gave:

Fukolam-S - 2.0-4.0

Natural honey - 50.0-70.0

Sorbic acid - 1.0-2.0

Succinic acid - 1.0-2.0,

as well as in l:

Infusion of rose hips and hawthorn fruits - 100.0-104.0

Tincture of the roots of Rhodiola rosea - 1.0-2.0

Water is the rest.

The drink was called "Fukovit."

Along with fucolam-C, plant extracts containing plant complex of physiologically active substances (antioxidants, carotenoids, flavonoids, etc.), plant adaptogenic acids of the Krebs cycle and honey increase the biological value of the drink.

The applicant also offers non-alcoholic drinks containing fucolam-C and a base selected from the range: drinking water, mineral water, juice, nectar, mineralized water, lemonade, fruit drink, kvass, non-alcoholic beer, in the following ratio, wt.%: Fucolam- C - up to 4.0, the basis - the rest.

Tests of the Fukovit drink were carried out by volunteers in the Department of Anesthesiology - Resuscitation of the Medical Association of the Far Eastern Branch of the Russian Academy of Sciences. Patients drank a drink of 100 ml once a day for 1 month. Based on the tests, the following was revealed: the Fukovit drink improves immunity, increases resistance to colds, does not change the hemostatic system, does not increase fibrinogen, and improves liver function. Moreover, it is not contraindicated for people with reduced blood coagulation, with thyroid disease. The invention expands the range of therapeutic drinks containing biologically active polysaccharides of brown algae.

A method of obtaining a drink "Fukovit" is as follows. An infusion is prepared from plant materials: hawthorn and rosehips. To do this, sorted, shredded and weighed air-dry raw materials are fed in a certain ratio to the infusion vat, into which water is poured (the water can be distilled, purified drinking, electrochemically activated or mineral). The infusion is obtained by boiling for 2 hours or keeping for 10 hours at 55-60 ° C. The infusion is filtered. The residue is washed with water. Infusion is served in the blending department. Prepare tincture of the roots of Rhodiola rosea by infusion in 40% ethanol at the rate of 100 g of roots per liter of extractant and served in the blending department.

The estimated amount of fucolam-C is dissolved in 50 l of hot water and served on the blend. Honey is preheated at 60 ° C for 2 hours. Cooked calculated quantities of ingredients are introduced into an enameled or stainless steel blended container; the contents are mixed for one hour. The drink is kept at room temperature for 10-15 hours. In the finished blend, the dry matter content, acidity and organoleptic characteristics are checked.

Blend served at the bottling. Bottling, briquetting, labeling of beverage bottles are carried out in accordance with generally accepted technological instructions for the production of soft drinks.

The drink according to organoleptic and physico-chemical indicators must meet the following requirements: solids content of 8-10 g per 100 g of drink; The pH of the drink is 6.0-7.2. Color - light brown, taste and aroma corresponds to a bouquet of plant materials.

As a result of solving the task, the applicant created cosmetics based on fucolam-C.

It has been established that many algae contain a wide range of biologically active substances, which have a positive effect on the condition of the skin and are therefore useful in cosmetics.

Describes the use as biologically active substances in the composition of cosmetics, freshly grown cells of Spirulina platensis [RU 2114632 C1, 07/10/1998], particles of dry biomass of kelp Laminaria saccarina or Laminaria Japonica [RU 2210359 C1, 08/20/2003], biogel from Laminaria Japonica [RU 2210359 C1, 08/20/2003], an extract of brown algae [RU 2189246 C1, 09/20/2002].

In order to create targeted cosmetics, they began to introduce not concentrates or extracts of brown algae into their compositions, but individual biologically active substances isolated from various algae. Moreover, the properties of these substances are affected by both the type of algae and the method of their preparation from algae.

A cosmetic product for external use is known containing 0.1-0.8% fucoidan obtained from sporophylls of the Undaria brown alga (WAKAME). The agent may contain either fucoidan or fucoidan itself in admixture with sodium alginate. A cream or lotion containing this substance is perfectly absorbed by the skin, protects it from ultraviolet rays, wind, low temperature, etc., supports the skin in a normal state [JP 1031707, 02.02.1989].

Almost simultaneously with the launch of the Apoidan-V® drink, the Japanese company Tarkarabio launched a lotion containing brown seaweed fucoidan K.crassifolia [Savago and Kato "Japan. J.Phycol. (Sorui)" 51: 19-25,2003, p .13].

A well-known cream for the skin of the face, which, along with the ingredients that form the basis of any cosmetic product, contains a fucoidan hydrolyzate from the alga Fucus vesiculosis (bubbly fucus), which in the known composition functions as a wound healing agent [RU 2246933 C1, 02.27.2005].

However, the immunomodulatory properties of the known cream are low, because the biological activity of fucoidan hydrolyzate, consisting of individual monomeric sugars, is reduced.

EFFECT: enhanced immunomodulatory properties of a cosmetic product due to the introduction of fucolam-C into the composition, expansion of the arsenal of immunocosmetological agents that slow down the aging process.

Perfume and cosmetic product contains fucolam-C and a cosmetic acceptable base for external use, selected from the following: fat-based module, emulsion-based module, gel-based module, water-alcohol-based module, in the following ratio of components, wt.% : fucolam-C - up to 6.0, cosmetic base - the rest.

Fukolam-S can be introduced into any perfumery-cosmetic base, depending on which cosmetic product you need to create. Moreover, the recommended percentage of input of this ingredient into a cosmetic product is from 1 to 6%. Tests have shown that at a higher concentration, the density of the cosmetic product increases, while at a lower concentration, the biological effectiveness of the cosmetic composition decreases.

Tests of the claimed cosmetics carried out on volunteers (10 people aged 18 to 60 years) in the Department of Anesthesiology and Resuscitation of the Medical Association of the Far Eastern Branch of the Russian Academy of Sciences showed that they exhibit anti-inflammatory, decongestant, anti-allergic effects. Means show an increased regenerative effect, because during skin healing, the simultaneous immunological protection and collagen synthesis leads to the activation of “skin immunity”. Physically and biologically, the skin is cleaned of impurities and regenerated with the help of newly synthesized collagen, while restoring skin elasticity, softness and elasticity.

The group of inventions is illustrated by the following examples of specific performance:

Example 1. The biologically active product is fucolam-C.

The product is obtained from brown alga Fucus evanescens, it contains a polysaccharide composition consisting of fucoidan in an amount of 70% and polymannuronic acid in an amount of 30%.

Physico-chemical characteristics of fucolam-C are presented in table 1.

Table 1 Name of indicator Indicator value Mass fraction of water,% 15.0 Mass fraction of fucoidan in%, calculated on absolutely dry weight 70.0 Mass fraction of polymannuronic acid in%, calculated on absolutely dry weight 30,0 Biological activity according to the stimulation index in the HCT test (test for the restoration of nitro blue tetrazolium by neutrophils of the peritoneal cavity of mice) 1.8 The content of sulfates (SO) ⁴ ,% 21.0 PH of 1% solution 6.2 Mass fraction of ash,% 22.0

Organoleptic characteristics of the product are presented in table 2.

table 2 Name of indicator (characteristic) Content Characteristics Appearance Homogeneous or fine fiber powder, light beige to brown Taste and smell Pleasant aroma of algae, without extraneous taste and smell The presence of impurities Not allowed

Indicators of the content of toxic elements, pesticides and radionuclides in fucolam-C are presented in table 3.

Table 3 Name of substance Indicator value one 2 Toxic elements, mg / kg Lead 0,086 Cadmium 0.19 Arsenic less than 0.03 Mercury 0.0012 Radionuclides, Bq / kg Cesium-137 less than 50.9 Strontium-90 less than 16.5 Pesticides mg / kg Hexachlorocyclohexane 0.01 DDT and its metabolites 0.012

Microbiological indicators of fucolam-C are presented in table 4.

Table 4 Name of indicator Indicator value The number of mesophilic aerobic and facultative anaerobic microorganisms, CFU / g Less than 1 × 10 ¹ BGKP (coliforms) in 0.1 g Not found Not found E. coli in 1.0 g Not found Not found S.aureus 1.0 g Not found Not found Pathogenic (including salmonella) in 10.0 g Not found Not found Mold and yeast, CFU / g, Less than 1 × 10 ¹

Example 2. BAA "Fukolam"

A 0.5 g capsule dietary supplement contains 100 mg of fucolam-C and 400 mg of calcium alginate.

Physico-chemical characteristics of dietary supplements "Fukolam" are presented in table 5.

Table 5 Name of indicator Indicator value Mass fraction of water,% 12.0 Mass fraction of fucolam-C,%, calculated on absolutely dry weight 17.0 Mass fraction of alginic acids,%, calculated on absolutely dry weight 51.0 Mass fraction of calcium,%, calculated on absolutely dry weight 8.0 Biological activity according to the stimulation index in the HCT test (test for the restoration of nitro blue tetrazolium by neutrophils of the peritoneal cavity of mice) 1.8 Mass fraction of ash,% 20,0

Example 3. The composition of the ingredients for the drink "Fukovit"

For the preparation of the drink, the consumption of constituent ingredients per 100 gave the following, kg:

Fukolam-S - 3.0

Natural honey - 60.0

Sorbic acid - 1.5

Succinic acid - 1.5,

as well as in l:

Infusion of rose hips and hawthorn fruits - 102.0

Tincture of the roots of Rhodiola rosea - 1.5

Water is the rest.

Example 4. Soft drink

To obtain 100 Dal of the drink, the components are taken in the following ratio (kg): fucolam-C - 4.0; and also in l: apple juice - 200.0, water - the rest.

Example 5. Perfume and cosmetic

Fat-based cream containing (wt.%): Fucolam-C - 5.0, paraffin - 0.5-1.5, olive oil - 0.5-1.5, pork fat - 1.0-2, 0, distilled monoglycerides - 6.5-7.5, beeswax - 0.5-1.5, coconut oil - 1.0-2.0, glycerin - 5.0-6.0, sulfonol 0.05- 0.15, sorbic acid 0.15-0.25, pectin - 0.5-1.5, potassium hydroxide - 0.01-0.03, fragrance 0.1-0.2, aqueous extract of Japanese kelp - up to one hundred.

The cream preparation process is carried out in an enameled container with a steam jacket and a stirrer. Japanese kelp extract, fucolam-C, pectin, sorbic acid are loaded into the container and heated with stirring. A fat base is loaded into a second enameled container and heated to 85-90 ° C, melting with continuous stirring. Pumped into the first container and emulsified for 30-60 minutes. The temperature is reduced to 55-60 ° C, the fragrance is charged and emulsified for 15-20 minutes. The finished emulsion is served in an enameled boiler for cooling with a refrigerator and a stirrer. Knead the mass for 25-30 minutes and cool to a temperature of 25-30 ° C.

The resulting cream is easily and evenly applied to the skin, quickly absorbed. The cream has a beneficial effect on the skin - turgor and skin elasticity increase, regeneration is enhanced, puffiness is removed and inflammation is eliminated, especially on irritated, sensitive and hypersensitive skin.

Example 6. Perfume and cosmetic

A water-alcohol based lotion containing (wt.%) Fucolam-C - 2.5, succinic acid - 0.1-0.2, boric acid - 0.1-0.2, sodium chloride - 0.8- 0.9, urea - 0.1-0.2, perfume - 0.1-0.2, ethanol - 5.0-6.0, water - up to 100.

To prepare the lotion, all the ingredients in accordance with the recipe are mixed with ethanol in a glass dish at room temperature, and then with water, mixed and filtered.

The lotion moisturizes the skin well, improves its smoothness and elasticity, reduces cellulite effects, as when it affects the skin, the lipid content in the problem areas of the subcutaneous fat layer decreases due to the activation of lipases that break down fats.

Claims (5)

1. A biologically active product containing polysaccharides of brown algae, characterized in that as polysaccharides of brown algae contains a polysaccharide composition obtained from alga Fucus evanescens, consisting of fucoidan in an amount of 60-80% and polymannuronic acid in an amount of 20-40%. 2. A biologically active food supplement containing polysaccharides of brown algae, characterized in that as the polysaccharides of brown algae contains the product according to claim 1 and calcium alginate in the following ratio of components, wt.%: biologically active product according to claim 1 twenty calcium alginate 80 3. The composition of the ingredients for a soft drink, characterized in that it contains the biologically active product according to claim 1, an infusion of rose hips and hawthorn fruits, tincture of the roots of Rhodiola rosea, sorbic acid, succinic acid, honey and water in the following ratio of ingredients, kg / 100 gave: biologically active product according to claim 1 2.0-4.0 natural honey 50.0-70.0 sorbic acid 1.0-2.0 succinic acid 1.0-2.0 as well as in l: infusion of rose hips and fruits hawthorn 100.0-104.0 tincture of the roots of Rhodiola rosea 1.0-2.0 water rest 4. A non-alcoholic drink, characterized in that it contains the biologically active product according to claim 1 and a base selected from the series: drinking water, mineral water, juice, nectar, mineralized water, lemonade, fruit drink, kvass, non-alcoholic beer or mixtures thereof, with the following ratio of components, wt.%: biologically active product according to claim 1 4.0 the basis rest 5. Perfume and cosmetic product, characterized in that it contains the biologically active product according to claim 1 and a perfume and cosmetic acceptable base for external use, selected from the following: a fat-based module, an emulsion-based module, a gel-based module, a module on water-alcohol based, in the following ratio of components, wt.%: biologically active product according to claim 1 6.0 perfumery and cosmetic base rest