RU2017124909A3 - - Google Patents
Download PDFInfo
- Publication number
- RU2017124909A3 RU2017124909A3 RU2017124909A RU2017124909A RU2017124909A3 RU 2017124909 A3 RU2017124909 A3 RU 2017124909A3 RU 2017124909 A RU2017124909 A RU 2017124909A RU 2017124909 A RU2017124909 A RU 2017124909A RU 2017124909 A3 RU2017124909 A3 RU 2017124909A3
- Authority
- RU
- Russia
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/105—Murine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462094104P | 2014-12-19 | 2014-12-19 | |
US62/094,104 | 2014-12-19 | ||
US201562167408P | 2015-05-28 | 2015-05-28 | |
US62/167,408 | 2015-05-28 | ||
US201562205524P | 2015-08-14 | 2015-08-14 | |
US62/205,524 | 2015-08-14 | ||
PCT/US2015/066681 WO2016100819A1 (en) | 2014-12-19 | 2015-12-18 | Methods and compositions for targeted genetic modification through single-step multiple targeting |
Publications (3)
Publication Number | Publication Date |
---|---|
RU2017124909A RU2017124909A (ru) | 2019-01-21 |
RU2017124909A3 true RU2017124909A3 (ru) | 2019-06-05 |
RU2707137C2 RU2707137C2 (ru) | 2019-11-22 |
Family
ID=55229831
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
RU2017124909A RU2707137C2 (ru) | 2014-12-19 | 2015-12-18 | Способы и композиции для нацеленной генетической модификации посредством одноэтапного множественного нацеливания |
Country Status (15)
Country | Link |
---|---|
US (2) | US11326184B2 (ru) |
EP (2) | EP3653048B9 (ru) |
JP (2) | JP6840077B2 (ru) |
KR (1) | KR102530821B1 (ru) |
CN (1) | CN107208113A (ru) |
AU (1) | AU2015364427B2 (ru) |
BR (1) | BR112017013104A2 (ru) |
CA (1) | CA2971213C (ru) |
ES (2) | ES2760508T3 (ru) |
IL (1) | IL252755B (ru) |
MX (1) | MX2017008190A (ru) |
NZ (1) | NZ732895A (ru) |
RU (1) | RU2707137C2 (ru) |
SG (1) | SG11201704646YA (ru) |
WO (1) | WO2016100819A1 (ru) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11201406547YA (en) | 2012-04-25 | 2014-11-27 | Regeneron Pharma | Nuclease-mediated targeting with large targeting vectors |
DK2986729T3 (en) | 2013-04-16 | 2018-10-29 | Regeneron Pharma | TARGETED MODIFICATION OF ROOT THROUGH |
RU2685914C1 (ru) | 2013-12-11 | 2019-04-23 | Регенерон Фармасьютикалс, Инк. | Способы и композиции для направленной модификации генома |
KR102374379B1 (ko) | 2014-06-06 | 2022-03-17 | 리제너론 파마슈티칼스 인코포레이티드 | 표적화된 좌를 변형시키는 방법 및 조성물 |
ES2901074T3 (es) | 2014-06-26 | 2022-03-21 | Regeneron Pharma | Métodos y composiciones para modificaciones genéticas objetivo y métodos de uso |
MX2017004890A (es) | 2014-10-15 | 2018-02-12 | Regeneron Pharma | Metodos y composiciones para la generación o el mantenimiento de células pluripotentes. |
WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
NZ731962A (en) | 2014-11-21 | 2022-07-01 | Regeneron Pharma | Methods and compositions for targeted genetic modification using paired guide rnas |
MX2017008190A (es) | 2014-12-19 | 2018-03-23 | Regeneron Pharma | Metodos y composiciones para la modificacion genetica dirigida a traves de direccionamiento multiple de una sola etapa. |
WO2016196185A1 (en) | 2015-05-29 | 2016-12-08 | Regeneron Pharmaceuticals, Inc. | Non-human animals having a disruption in a c9orf72 locus |
US11667911B2 (en) | 2015-09-24 | 2023-06-06 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/CAS-mediated genome editing |
EP4257599A3 (en) | 2016-01-13 | 2024-01-17 | Regeneron Pharmaceuticals, Inc. | Rodents having an engineered heavy chain diversity region |
US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
JP7026678B2 (ja) | 2016-09-30 | 2022-02-28 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | C9orf72座位中にヘキサヌクレオチドリピート伸長を有する非ヒト動物 |
CN109996441B (zh) | 2016-11-04 | 2022-02-08 | 瑞泽恩制药公司 | 具有经工程化的免疫球蛋白λ轻链基因座的非人类动物 |
AU2018208463A1 (en) * | 2017-01-10 | 2019-08-01 | Christina Care Gene Editing Institute, Inc. | Methods for in vitro site-directed mutagenesis using gene editing technologies |
WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
SG11201912236YA (en) * | 2017-07-31 | 2020-01-30 | Regeneron Pharma | Crispr reporter non-human animals and uses thereof |
CN111432635B (zh) | 2017-12-05 | 2022-10-21 | 瑞泽恩制药公司 | 具有经工程改造的免疫球蛋白λ轻链的非人动物以及所述非人动物的用途 |
US20190380316A1 (en) | 2018-06-14 | 2019-12-19 | Regeneron Pharmaceuticals, Inc. | Non-human animals capable of engineered dh-dh rearrangement and uses thereof |
CN111321171A (zh) * | 2018-12-14 | 2020-06-23 | 江苏集萃药康生物科技有限公司 | 一种应用CRISPR/Cas9介导ES打靶技术制备基因打靶动物模型的方法 |
EP3732291A1 (en) | 2018-12-20 | 2020-11-04 | Regeneron Pharmaceuticals, Inc. | Nuclease-mediated repeat expansion |
IL286917B (en) * | 2019-04-04 | 2022-09-01 | Regeneron Pharma | Methods for scar-free insertion of targeted modifications into targeted vectors |
TW202110323A (zh) | 2019-06-05 | 2021-03-16 | 美商再生元醫藥公司 | 具有表現自κ 基因座的有限λ 輕鏈組庫的非人類動物及其用途 |
EP4051700A1 (en) | 2020-12-23 | 2022-09-07 | Regeneron Pharmaceuticals, Inc. | Nucleic acids encoding anchor modified antibodies and uses thereof |
Family Cites Families (94)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5612205A (en) | 1990-08-29 | 1997-03-18 | Genpharm International, Incorporated | Homologous recombination in mammalian cells |
US5830729A (en) | 1996-04-18 | 1998-11-03 | Institut Pasteur | I Sce I-induced gene replacement and gene conversion in embryonic stem cells |
AU8587598A (en) | 1997-07-26 | 1999-02-16 | Wisconsin Alumni Research Foundation | Trans-species nuclear transfer |
US6372956B1 (en) | 1998-12-31 | 2002-04-16 | The J. David Gladstone Institutes | Transgenic rats and rat cell lines expressing human CD4 and a human chemokine receptor |
US6599692B1 (en) | 1999-09-14 | 2003-07-29 | Sangamo Bioscience, Inc. | Functional genomics using zinc finger proteins |
US20030104526A1 (en) | 1999-03-24 | 2003-06-05 | Qiang Liu | Position dependent recognition of GNN nucleotide triplets by zinc fingers |
IL150069A0 (en) | 1999-12-06 | 2002-12-01 | Sangamo Biosciences Inc | Methods of using randomized libraries of zinc finger proteins for the identification of gene function |
US7105348B2 (en) * | 2000-10-31 | 2006-09-12 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
US6586251B2 (en) | 2000-10-31 | 2003-07-01 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
US6596541B2 (en) | 2000-10-31 | 2003-07-22 | Regeneron Pharmaceuticals, Inc. | Methods of modifying eukaryotic cells |
US20050144655A1 (en) | 2000-10-31 | 2005-06-30 | Economides Aris N. | Methods of modifying eukaryotic cells |
AU2002228841C1 (en) | 2000-12-07 | 2006-11-23 | Sangamo Biosciences, Inc | Regulation of angiogenesis with zinc finger proteins |
WO2002057308A2 (en) | 2001-01-22 | 2002-07-25 | Sangamo Biosciences, Inc. | Zinc finger polypeptides and their use |
US7273923B2 (en) | 2001-01-22 | 2007-09-25 | Sangamo Biosciences, Inc. | Zinc finger proteins for DNA binding and gene regulation in plants |
AUPR451401A0 (en) | 2001-04-20 | 2001-05-24 | Monash University | A method of nuclear transfer |
CA2474486C (en) | 2002-01-23 | 2013-05-14 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
WO2003078619A1 (en) | 2002-03-15 | 2003-09-25 | Cellectis | Hybrid and single chain meganucleases and use thereof |
AU2003218382B2 (en) | 2002-03-21 | 2007-12-13 | Sangamo Therapeutics, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
US7612250B2 (en) | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
AU2003298574B2 (en) | 2002-09-05 | 2008-04-24 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
US20030175968A1 (en) | 2002-10-30 | 2003-09-18 | Golic Kent G. | Gene targeting method |
EP1587545A2 (en) | 2003-01-13 | 2005-10-26 | Mahendra S. Rao | Persistent expression of candidate molecule in proliferating stem and progenitor cells for delivery of therapeutic products |
US8409861B2 (en) | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
US20060063231A1 (en) | 2004-09-16 | 2006-03-23 | Sangamo Biosciences, Inc. | Compositions and methods for protein production |
EP2767161B1 (en) | 2004-10-19 | 2018-02-07 | Regeneron Pharmaceuticals, Inc. | Method for generating an non-human animal homozygous for a genetic modification |
FR2879622B1 (fr) | 2004-12-17 | 2008-02-01 | Agronomique Inst Nat Rech | Procede in vitro de production d'ovocytes ou d'oeufs presentant une modification genomique ciblee |
WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
AU2006224248B2 (en) | 2005-03-15 | 2011-01-06 | Cellectis | I-Crei meganuclease variants with modified specificity, method of preparation and uses thereof |
GB0615327D0 (en) | 2006-03-30 | 2006-09-13 | Univ Edinburgh | Culture medium containing kinase inhibitors and uses thereof |
EP2505058A1 (en) | 2006-03-31 | 2012-10-03 | Medarex, Inc. | Transgenic animals expressing chimeric antibodies for use in preparing human antibodies |
CN101117633B (zh) | 2006-08-03 | 2011-07-20 | 上海交通大学附属儿童医院 | 一种细胞核移植方法 |
HUE029238T2 (en) | 2006-12-14 | 2017-02-28 | Dow Agrosciences Llc | Optimized non-canonical zinc finger proteins |
US7771967B2 (en) | 2006-12-22 | 2010-08-10 | The J. David Gladstone Institutes | Nucleic acid encoding apolipoprotein E-I3 |
ATE489465T1 (de) | 2007-04-26 | 2010-12-15 | Sangamo Biosciences Inc | Gezielte integration in die ppp1r12c-position |
AU2008259939B2 (en) | 2007-06-01 | 2014-03-13 | Open Monoclonal Technology, Inc. | Compositions and methods for inhibiting endogenous immunoglobulin genes and producing transgenic human idiotype antibodies |
GB0803109D0 (en) | 2008-02-20 | 2008-03-26 | Gene Bridges Gmbh | Method of nucleic acid recombination |
WO2010065123A1 (en) | 2008-12-04 | 2010-06-10 | Sangamo Biosciences, Inc. | Genome editing in rats using zinc-finger nucleases |
EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
EP2408921B1 (en) | 2009-03-20 | 2017-04-19 | Sangamo BioSciences, Inc. | Modification of cxcr4 using engineered zinc finger proteins |
US8772008B2 (en) | 2009-05-18 | 2014-07-08 | Sangamo Biosciences, Inc. | Methods and compositions for increasing nuclease activity |
WO2011017293A2 (en) | 2009-08-03 | 2011-02-10 | The General Hospital Corporation | Engineering of zinc finger arrays by context-dependent assembly |
CA2779337A1 (en) | 2009-10-28 | 2011-05-05 | Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Fuer Gesundheit U Nd Umwelt (Gmbh) | Homologous recombination in the oocyte |
TR201903376T4 (tr) | 2009-10-29 | 2019-04-22 | Regeneron Pharma | Çok fonksiyonlu alleller. |
WO2011053957A2 (en) | 2009-11-02 | 2011-05-05 | Gen9, Inc. | Compositions and methods for the regulation of multiple genes of interest in a cell |
US9309537B2 (en) | 2009-12-01 | 2016-04-12 | National Cancer Center | Chimeric rat produced using rat embryonic stem cells in the presence of an ES cell differentiation suppressant |
PT2816112T (pt) | 2009-12-10 | 2018-11-20 | Univ Iowa State Res Found Inc | Modificação do adn modificada pelo efector tal |
CN102791865B (zh) | 2009-12-21 | 2015-07-01 | 凯津公司 | 用于转染原生质体的改进技术 |
EP2534163B1 (en) | 2010-02-09 | 2015-11-04 | Sangamo BioSciences, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
AU2011265306C1 (en) | 2010-06-11 | 2015-01-22 | Regeneron Pharmaceuticals, Inc. | Production of fertile XY female animals from XY ES cells |
JP6050230B2 (ja) | 2010-07-21 | 2016-12-21 | サンガモ バイオサイエンシーズ, インコーポレイテッド | Hla遺伝子座の修飾のための方法及び組成物 |
JP2013537410A (ja) | 2010-07-23 | 2013-10-03 | シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | 標的化エンドヌクレアーゼおよび一本鎖核酸を用いたゲノム編集 |
WO2012018726A1 (en) | 2010-08-02 | 2012-02-09 | Cellectis Sa | Method for increasing double-strand break-induced gene targeting |
WO2012129198A1 (en) | 2011-03-23 | 2012-09-27 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for obesity and diabetes |
KR102148387B1 (ko) | 2011-10-28 | 2020-08-26 | 리제너론 파아마슈티컬스, 인크. | 유전자 변형된 t 세포 수용체 마우스 |
WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
SG11201406547YA (en) | 2012-04-25 | 2014-11-27 | Regeneron Pharma | Nuclease-mediated targeting with large targeting vectors |
JP6559063B2 (ja) | 2012-05-07 | 2019-08-14 | サンガモ セラピューティクス, インコーポレイテッド | 導入遺伝子のヌクレアーゼ媒介標的化組み込みのための方法および組成物 |
ES2960803T3 (es) | 2012-05-25 | 2024-03-06 | Univ California | Métodos y composiciones para la modificación de ADN diana dirigida por RNA y para la modulación de la transcripción dirigida por RNA |
ES2926021T3 (es) | 2012-10-23 | 2022-10-21 | Toolgen Inc | Composición para escindir un ADN objetivo que comprende un ARN guía específico para el ADN objetivo y ácido nucleico codificador de proteína Cas o proteína Cas, y uso de la misma |
EP3617309A3 (en) | 2012-12-06 | 2020-05-06 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
SG10201912328UA (en) | 2012-12-12 | 2020-02-27 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications |
CN113355357A (zh) | 2012-12-12 | 2021-09-07 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
DK2931030T4 (da) * | 2012-12-14 | 2024-04-22 | Omniab Inc | Polynukleotider, der koder for graver-antistoffer med humane idiotyper, og dyr, der omfatter disse |
IL308158A (en) | 2012-12-17 | 2023-12-01 | Harvard College | RNA-guided human genome engineering |
DK3491915T3 (da) | 2012-12-27 | 2023-08-28 | Keygene Nv | Fremgangsmåde til at inducere en målrettet translokation i en plante |
MX2015010841A (es) | 2013-02-20 | 2016-05-09 | Regeneron Pharma | Modificacion genetica de ratas. |
EP2922393B2 (en) | 2013-02-27 | 2022-12-28 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Gene editing in the oocyte by cas9 nucleases |
WO2014143381A1 (en) | 2013-03-09 | 2014-09-18 | Agilent Technologies, Inc. | Methods of in vivo engineering of large sequences using multiple crispr/cas selections of recombineering events |
US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
CA2906970C (en) | 2013-03-21 | 2021-05-18 | Ospedale San Raffaele Srl | Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases |
DK2986729T3 (en) * | 2013-04-16 | 2018-10-29 | Regeneron Pharma | TARGETED MODIFICATION OF ROOT THROUGH |
DE202014010413U1 (de) | 2013-09-18 | 2015-12-08 | Kymab Limited | Zellen und Organismen |
US10787684B2 (en) | 2013-11-19 | 2020-09-29 | President And Fellows Of Harvard College | Large gene excision and insertion |
RU2685914C1 (ru) | 2013-12-11 | 2019-04-23 | Регенерон Фармасьютикалс, Инк. | Способы и композиции для направленной модификации генома |
AU2014368982B2 (en) | 2013-12-19 | 2021-03-25 | Amyris, Inc. | Methods for genomic integration |
KR101823661B1 (ko) | 2014-04-24 | 2018-01-30 | 기초과학연구원 | 마이크로 상동 기반의 유전자 녹아웃을 위한 뉴클레아제 표적 서열을 확인하는 방법 |
GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
KR102374379B1 (ko) | 2014-06-06 | 2022-03-17 | 리제너론 파마슈티칼스 인코포레이티드 | 표적화된 좌를 변형시키는 방법 및 조성물 |
ES2901074T3 (es) | 2014-06-26 | 2022-03-21 | Regeneron Pharma | Métodos y composiciones para modificaciones genéticas objetivo y métodos de uso |
US20160081314A1 (en) * | 2014-09-19 | 2016-03-24 | Regeneron Pharmaceuticals, Inc. | Chimeric Antigen Receptors |
WO2016061073A1 (en) | 2014-10-14 | 2016-04-21 | Memorial Sloan-Kettering Cancer Center | Composition and method for in vivo engineering of chromosomal rearrangements |
MX2017004890A (es) | 2014-10-15 | 2018-02-12 | Regeneron Pharma | Metodos y composiciones para la generación o el mantenimiento de células pluripotentes. |
EP3222718A4 (en) | 2014-11-20 | 2018-05-30 | Kyoto University | Method for knock-in of dna into target region of mammalian genome, and cell |
NZ731962A (en) | 2014-11-21 | 2022-07-01 | Regeneron Pharma | Methods and compositions for targeted genetic modification using paired guide rnas |
WO2016089866A1 (en) | 2014-12-01 | 2016-06-09 | President And Fellows Of Harvard College | Rna-guided systems for in vivo gene editing |
MX2017008190A (es) | 2014-12-19 | 2018-03-23 | Regeneron Pharma | Metodos y composiciones para la modificacion genetica dirigida a traves de direccionamiento multiple de una sola etapa. |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
-
2015
- 2015-12-18 MX MX2017008190A patent/MX2017008190A/es unknown
- 2015-12-18 BR BR112017013104A patent/BR112017013104A2/pt not_active Application Discontinuation
- 2015-12-18 WO PCT/US2015/066681 patent/WO2016100819A1/en active Application Filing
- 2015-12-18 US US14/974,623 patent/US11326184B2/en active Active
- 2015-12-18 KR KR1020177019826A patent/KR102530821B1/ko active IP Right Grant
- 2015-12-18 ES ES15828604T patent/ES2760508T3/es active Active
- 2015-12-18 SG SG11201704646YA patent/SG11201704646YA/en unknown
- 2015-12-18 EP EP19201384.5A patent/EP3653048B9/en active Active
- 2015-12-18 NZ NZ732895A patent/NZ732895A/en unknown
- 2015-12-18 EP EP15828604.7A patent/EP3232774B1/en active Active
- 2015-12-18 CN CN201580069507.5A patent/CN107208113A/zh active Pending
- 2015-12-18 CA CA2971213A patent/CA2971213C/en active Active
- 2015-12-18 ES ES19201384T patent/ES2947714T3/es active Active
- 2015-12-18 JP JP2017532808A patent/JP6840077B2/ja active Active
- 2015-12-18 AU AU2015364427A patent/AU2015364427B2/en active Active
- 2015-12-18 RU RU2017124909A patent/RU2707137C2/ru active
-
2017
- 2017-06-07 IL IL252755A patent/IL252755B/en unknown
-
2020
- 2020-12-28 JP JP2020219029A patent/JP7095066B2/ja active Active
-
2022
- 2022-04-05 US US17/713,696 patent/US20220235381A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
ES2947714T3 (es) | 2023-08-17 |
SG11201704646YA (en) | 2017-07-28 |
AU2015364427A1 (en) | 2017-07-06 |
JP2017538428A (ja) | 2017-12-28 |
CN107208113A (zh) | 2017-09-26 |
NZ732895A (en) | 2022-05-27 |
IL252755B (en) | 2021-10-31 |
KR20170093246A (ko) | 2017-08-14 |
ES2947714T9 (es) | 2024-03-14 |
EP3232774A1 (en) | 2017-10-25 |
CA2971213C (en) | 2023-09-26 |
US20160177339A1 (en) | 2016-06-23 |
US20220235381A1 (en) | 2022-07-28 |
JP2021045174A (ja) | 2021-03-25 |
KR102530821B1 (ko) | 2023-05-10 |
AU2015364427B2 (en) | 2021-05-27 |
EP3653048B1 (en) | 2023-06-07 |
JP6840077B2 (ja) | 2021-03-10 |
EP3653048C0 (en) | 2023-06-07 |
CA2971213A1 (en) | 2016-06-23 |
MX2017008190A (es) | 2018-03-23 |
JP7095066B2 (ja) | 2022-07-04 |
ES2760508T3 (es) | 2020-05-14 |
EP3232774B1 (en) | 2019-10-09 |
IL252755A0 (en) | 2017-08-31 |
US11326184B2 (en) | 2022-05-10 |
EP3653048B9 (en) | 2023-10-04 |
RU2707137C2 (ru) | 2019-11-22 |
WO2016100819A1 (en) | 2016-06-23 |
RU2017124909A (ru) | 2019-01-21 |
BR112017013104A2 (pt) | 2018-05-15 |
EP3653048A1 (en) | 2020-05-20 |