RU2016102922A - Способы идентификации, изоляции и применения рецепторов пахучих веществ и ароматов - Google Patents
Способы идентификации, изоляции и применения рецепторов пахучих веществ и ароматов Download PDFInfo
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- RU2016102922A RU2016102922A RU2016102922A RU2016102922A RU2016102922A RU 2016102922 A RU2016102922 A RU 2016102922A RU 2016102922 A RU2016102922 A RU 2016102922A RU 2016102922 A RU2016102922 A RU 2016102922A RU 2016102922 A RU2016102922 A RU 2016102922A
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
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- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
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Claims (19)
1. Способ идентификации обонятельных рецепторов, активируемых пахучим и/или ароматическим соединением, включающий:
(a) диссоциацию изолированного обонятельного эпителия, содержащего нативные обонятельные нейроны, на отдельные клетки от видов млекопитающих, не являющихся человеком, где каждый нейрон экспрессирует обонятельный рецептор;
(b) загрузку обонятельных клеток индикаторным красителем, позволяющим проводить измерение связывающей активности рецепторов веществ с неприятным запахом для обонятельных рецепторов;
(c) обеспечение контакта обонятельных рецепторов с соединениями с неприятным запахом;
(d) измерение изменений активности нейронов, индуцированной пахучими веществами;
(e) изоляцию одного или нескольких обонятельных нейронов, активированных соединением с неприятным запахом;
(f) амплификацию мРНК из изолированных обонятельных рецепторов;
(g) секвенирование по меньшей мере части всего транскриптома мРНК посредством секвенирования нового поколения; и
(h) определение идентичности группы обонятельных рецепторов неприятных запахов путем сравнения последовательности транскриптома с эталонной геномной последовательностью тех же самых видов.
2. Способ по п. 1, дополнительно включающий идентификацию человеческого рецептора, наиболее близко родственного обонятельному рецептору неприятных запахов, идентифицированному в п. 1.
3. Способ по п. 1, дополнительно включающий модификацию клеточной линии для экспрессии по меньшей мере одного обонятельного рецептора неприятных запахов, идентифицированного в п. 1.
4. Способ по п. 2, дополнительно включающий модификацию клеточной линии для экспрессии по меньшей мере человеческого рецептора, идентифицированного в п. 2.
5. Способ по п. 3, дополнительно включающий обеспечение контакта обонятельного рецептора неприятных запахов с тестируемым соединением, чтобы определить, связывается ли тестируемое соединение с обонятельным рецептором неприятных запахов.
6. Способ по п. 4, дополнительно включающий обеспечение контакта человеческого рецептора с тестируемым соединением, чтобы определить, связывается ли тестируемое соединение с рецептором.
7. Клетка, рекомбинантно модифицированная для экспрессии полипептида, включающего аминокислотную последовательность, имеющую по меньшей мере 60%-ную идентичность последовательности с аминокислотной последовательностью, выбранной из группы, состоящей из SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14 SEQ ID, и SEQ ID NO: 16,, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 46, SEQ ID NO: 48, SEQ ID NO: 50, SEQ ID NO: 52, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, SEQ ID NO: 60, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO 76, SEQ ID NO 78, SEQ ID NO 80, SEQ ID NO 82, SEQ ID NO 84 и SEQ ID NO 86.
8. Клетка по п. 7, выбранная из группы, состоящей из НЕК293, СНО, ооцитов Xenopus, COS, дрожжей, бактерий и клеток, полученных из обонятельной плакоды.
9. Способ идентификации соединения, которое блокирует, ингибирует, модулирует, и/или повышает активность обонятельного рецептора, выбранного из группы, состоящей из рецепторов пахучих или ароматических веществ; который активируется соединением, выбранным из группы, состоящей из индола и скатола, включающий:
(a) обеспечение контакта рецептора, или его химеры или фрагмента, с соединением, где рецептор является полипептидом включающим аминокислотную последовательность, имеющую по меньшей мере 60%-ную идентичность последовательности с аминокислотной последовательностью, выбранной из группы, состоящей из SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14 SEQ ID, и SEQ ID NO: 16,, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 46, SEQ ID NO: 48, SEQ ID NO: 50, SEQ ID NO: 52, SEQ ID NO: 54, SEQ ID NO: 56, SEQ ID NO: 58, SEQ ID NO: 60, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO 76, SEQ ID NO 78, SEQ ID NO 80, SEQ ID NO 82, SEQ ID NO 84 и SEQ ID NO 86;
(b) измерение степени, в которой соединение блокирует, ингибирует, модулирует или повышает активность рецептора.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361841282P | 2013-06-29 | 2013-06-29 | |
| US61/841,282 | 2013-06-29 | ||
| US201361921664P | 2013-12-30 | 2013-12-30 | |
| US61/921,664 | 2013-12-30 | ||
| PCT/US2014/044770 WO2014210585A2 (en) | 2013-06-29 | 2014-06-28 | Methods of identifying, isolating and using odorant and aroma receptors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2016102922A true RU2016102922A (ru) | 2017-08-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2016102922A RU2016102922A (ru) | 2013-06-29 | 2014-06-28 | Способы идентификации, изоляции и применения рецепторов пахучих веществ и ароматов |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US9914760B2 (ru) |
| EP (1) | EP3013978B1 (ru) |
| JP (2) | JP2016523546A (ru) |
| CN (1) | CN105339509A (ru) |
| BR (1) | BR112015032700B1 (ru) |
| ES (1) | ES2768338T3 (ru) |
| MX (1) | MX371282B (ru) |
| RU (1) | RU2016102922A (ru) |
| WO (1) | WO2014210585A2 (ru) |
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2014
- 2014-06-28 ES ES14745271T patent/ES2768338T3/es active Active
- 2014-06-28 WO PCT/US2014/044770 patent/WO2014210585A2/en active Application Filing
- 2014-06-28 US US14/318,641 patent/US9914760B2/en active Active
- 2014-06-28 JP JP2016524276A patent/JP2016523546A/ja active Pending
- 2014-06-28 MX MX2015017943A patent/MX371282B/es active IP Right Grant
- 2014-06-28 CN CN201480036935.3A patent/CN105339509A/zh active Pending
- 2014-06-28 RU RU2016102922A patent/RU2016102922A/ru not_active Application Discontinuation
- 2014-06-28 BR BR112015032700-1A patent/BR112015032700B1/pt active IP Right Grant
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2018
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| US20150005177A1 (en) | 2015-01-01 |
| MX371282B (es) | 2020-01-24 |
| CN105339509A (zh) | 2016-02-17 |
| MX2015017943A (es) | 2016-05-10 |
| US9914760B2 (en) | 2018-03-13 |
| BR112015032700B1 (pt) | 2022-12-20 |
| ES2768338T3 (es) | 2020-06-22 |
| JP2019004883A (ja) | 2019-01-17 |
| WO2014210585A3 (en) | 2015-04-16 |
| EP3013978B1 (en) | 2019-11-20 |
| BR112015032700A2 (ru) | 2017-08-22 |
| JP2016523546A (ja) | 2016-08-12 |
| WO2014210585A2 (en) | 2014-12-31 |
| EP3013978A2 (en) | 2016-05-04 |
| JP6835773B2 (ja) | 2021-02-24 |
| US20180208637A1 (en) | 2018-07-26 |
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