RU2015129762A - ОДНОЛИНКЕРНЫЕ АНТИТЕЛА FabFv И СПОСОБЫ ИХ ПОЛУЧЕНИЯ - Google Patents
ОДНОЛИНКЕРНЫЕ АНТИТЕЛА FabFv И СПОСОБЫ ИХ ПОЛУЧЕНИЯ Download PDFInfo
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Claims (85)
1. Молекула мультиспецифического антитела, содержащая или состоящая из трех полипептидов:
a) полипептидной цепи формулы (I)
(Vxx)nVx-Cx-X-V1 и
b) полипептидной цепи формулы (II)
(Vyy)nVy-Cy;
c) полипептида формулы (III)
V2,
где Vx - вариабельный домен,
Vxx - вариабельный домен,
Сх - константную область,
X - линкер,
V1 - вариабельный домен,
Vy - вариабельный домен,
Vyy - вариабельный домен,
Cy - константную область,
V2 - вариабельный домен,
n независимо равен 0 или 1,
где полипептидная цепь формулы (I) и полипептидная цепь формулы (II) выровнены так, что происходит спаривание константных областей Сх и Cy и спаривание вариабельных доменов Vx и Vy с образованием связывающего домена и, необязательно, дисульфидной связи между V1 и V2, в частности, если дисульфидная связь присутствует.
2. Молекула биспецифического антитела, содержащая или состоящая из трех полипептидов:
a) тяжелой цепи формулы (Ia)
VH-CH1-X-V1 и
b) легкой цепи формулы (IIa)
VL-CL;
c) полипептида формулы (III)
V2,
где VH - вариабельный домен тяжелой цепи,
CH1 - домен 1 константной области тяжелой цепи,
X - линкер,
V1 - вариабельный домен,
VL - вариабельный домен легкой цепи,
CL - константную область легкой цепи,
V2 - вариабельный домен,
где между V1 и V2 необязательно присутствует дисульфидная связь, в частности если дисульфидная связь присутствует.
3. Молекула биспецифического антитела, содержащая или состоящая из трех полипептидов:
a) тяжелой цепи формулы (Ib)
VH-CH1 и
b) легкой цепи формулы (IIb)
VL-CL-X-V2;
c) полипептида формулы (III)
V1,
где VH - вариабельный домен тяжелой цепи,
СН1 - домен 1 константной области тяжелой цепи,
X - линкер,
V1 - вариабельный домен,
VL - вариабельный домен легкой цепи,
CL - константную область легкой цепи,
V2 - вариабельный домен,
где между V1 и V2 необязательно присутствует дисульфидная связь, в частности если дисульфидная связь присутствует.
4. Молекула мультиспецифического антитела по п. 1,
где V1 - вариабельный домен тяжелой цепи, а
V2 - вариабельный домен легкой цепи.
5. Молекула мультиспецифического антитела по 1,
где пара вариабельных доменов V1/V2 связана дисульфидной связью между двумя остатками цистеина, введенными рекомбинантным методом, один в V1 и один в V2,
где положение пары остатков цистеина, введенных рекомбинатным методом, выбрано из группы, состоящей из VH37 и VL95, VH44 и VL100, VH44 и VL105, VH45 и VL87, VH100 и VL50, VH100b и VL49, VH98 и VL46, VH101 и VL46, VH105 и VL43 и VH106 и VL57.
6. Молекула мультиспецифического антитела по п. 1,
где пара вариабельных доменов V1/V2 обладает специфичностью в отношении сывороточного белка-носителя.
7. Молекула мультиспецифического антитела по п. 6,
где V1 содержит
последовательность SEQ ID NO: 87 для CDRH-1,
последовательность SEQ ID NO: 88 для CDRH2 и
последовательность SEQ ID NO: 89 для CDRH-3 и
V2 содержит
последовательность SEQ ID NO: 90 для CDRL-1,
последовательность SEQ ID NO: 91 для CDRL2 и
последовательность SEQ ID NO: 92 для CDRL-3.
8. Молекула мультиспецифического антитела по п. 6,
где V1 содержит
последовательность SEQ ID NO: 93 для CDRH-1,
последовательность SEQ ID NO: 94 для CDRH2 и
последовательность SEQ ID NO: 95 для CDRH-3 и
V2 содержит
последовательность SEQ ID NO: 96 для CDRL-1,
последовательность SEQ ID NO: 97 для CDRL2 и
последовательность SEQ ID NO: 98 для CDRL-3.
9. Молекула мультиспецифического антитела по п. 1,
где X имеет последовательность SEQ ID NO: 78.
10. Молекула биспецифического антитела по п. 3, где X имеет последовательность SEQ ID NO: 103.
11. Полинуклеотид, кодирующий молекулу мультиспецифического или биспецифического антитела по любому из пп. 1-10.
12. Вектор, содержащий полинуклеотид по п. 11.
13. Клетка-хозяин, содержащая вектор или полинуклеотид по п. 12 или 11 соответственно.
14. Клетка-хозяин, содержащая три вектора, где каждый вектор содержит полинуклеотид, кодирующий отдельную полипептидную цепь молекулы мультиспецифического или биспецифического антитела по любому одному из пп. 1-10.
15. Способ, включающий экспрессию молекулы мультиспецифического или биспецифического антитела в клетке-хозяине по п. 13 или 14.
16. Фармацевтическая композиция, содержащая молекулу мультиспецифического или биспецифического антитела по любому из пп. 1-10 и по меньшей мере один наполнитель.
17. Молекула мультиспецифического или биспецифического антитела по любому из пп. 1-10 или фармацевтическая композиция по п. 16 для применения в медицине.
18. Способ лечения пациента, нуждающегося в этом, включающий введение терапевтически эффективного количества молекулы мультиспецифического или биспецифического антитела по любому из пп. 1-10 или фармацевтической композиции по п. 16.
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Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104583239B (zh) | 2012-05-10 | 2018-09-18 | 生物蛋白有限公司 | 多特异单克隆抗体 |
WO2014022540A1 (en) * | 2012-08-02 | 2014-02-06 | Regeneron Pharmaceuticals, Inc. | Multivalent antigen-binding proteins |
GB201223276D0 (en) * | 2012-12-21 | 2013-02-06 | Ucb Pharma Sa | Antibodies and methods of producing same |
GB201409558D0 (en) | 2014-05-29 | 2014-07-16 | Ucb Biopharma Sprl | Method |
GB201411320D0 (en) | 2014-06-25 | 2014-08-06 | Ucb Biopharma Sprl | Antibody construct |
GB201412659D0 (en) | 2014-07-16 | 2014-08-27 | Ucb Biopharma Sprl | Molecules |
GB201412658D0 (en) | 2014-07-16 | 2014-08-27 | Ucb Biopharma Sprl | Molecules |
GB201506869D0 (en) * | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
GB201506868D0 (en) | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method for protein purification |
GB201601077D0 (en) | 2016-01-20 | 2016-03-02 | Ucb Biopharma Sprl | Antibody molecule |
GB201601075D0 (en) | 2016-01-20 | 2016-03-02 | Ucb Biopharma Sprl | Antibodies molecules |
GB201601073D0 (en) | 2016-01-20 | 2016-03-02 | Ucb Biopharma Sprl | Antibodies |
GB201521389D0 (en) | 2015-12-03 | 2016-01-20 | Ucb Biopharma Sprl | Method |
GB201521383D0 (en) | 2015-12-03 | 2016-01-20 | Ucb Biopharma Sprl And Ucb Celltech | Method |
GB201521382D0 (en) | 2015-12-03 | 2016-01-20 | Ucb Biopharma Sprl | Antibodies |
GB201521393D0 (en) | 2015-12-03 | 2016-01-20 | Ucb Biopharma Sprl | Antibodies |
GB201521391D0 (en) | 2015-12-03 | 2016-01-20 | Ucb Biopharma Sprl | Antibodies |
JP7133544B2 (ja) | 2016-05-01 | 2022-09-08 | ユーシービー バイオファルマ エスアールエル | 親和性を操作した血清タンパク質担体結合ドメイン |
GB201621591D0 (en) * | 2016-12-19 | 2017-02-01 | Ucb Biopharma Sprl | Antibody molecules and method |
CA3067835A1 (en) * | 2017-06-22 | 2018-12-27 | Apexigen, Inc. | Anti-vista antibodies and methods of use |
US11965030B2 (en) | 2018-12-24 | 2024-04-23 | Sanofi | Multispecific binding proteins with mutant fab domains |
GB201919062D0 (en) | 2019-12-20 | 2020-02-05 | Ucb Biopharma Sprl | Antibody |
GB201919058D0 (en) | 2019-12-20 | 2020-02-05 | Ucb Biopharma Sprl | Multi-specific antibodies |
GB202001447D0 (en) | 2020-02-03 | 2020-03-18 | Ucb Biopharma Sprl | Antibodies |
US20240024520A1 (en) | 2020-03-27 | 2024-01-25 | UCB Biopharma SRL | Autonomous knob domain peptides |
US20230374148A1 (en) | 2020-10-15 | 2023-11-23 | UCB Biopharma SRL | Binding molecules that multimerise cd45 |
JP2023551981A (ja) | 2020-12-07 | 2023-12-13 | ユーシービー バイオファルマ エスアールエル | 多重特異性抗体及び抗体の組み合わせ |
GB202111905D0 (en) | 2021-08-19 | 2021-10-06 | UCB Biopharma SRL | Antibodies |
Family Cites Families (69)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4741900A (en) | 1982-11-16 | 1988-05-03 | Cytogen Corporation | Antibody-metal ion complexes |
GB8422238D0 (en) | 1984-09-03 | 1984-10-10 | Neuberger M S | Chimeric proteins |
DK336987D0 (da) | 1987-07-01 | 1987-07-01 | Novo Industri As | Immobiliseringsmetode |
GB8719042D0 (en) | 1987-08-12 | 1987-09-16 | Parker D | Conjugate compounds |
GB8720833D0 (en) | 1987-09-04 | 1987-10-14 | Celltech Ltd | Recombinant dna product |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
EP1892296A1 (en) | 1988-09-02 | 2008-02-27 | Dyax Corporation | Generation and selection of recombinant varied binding proteins |
GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
GB8907617D0 (en) | 1989-04-05 | 1989-05-17 | Celltech Ltd | Drug delivery system |
GB8928874D0 (en) | 1989-12-21 | 1990-02-28 | Celltech Ltd | Humanised antibodies |
WO1991010737A1 (en) | 1990-01-11 | 1991-07-25 | Molecular Affinities Corporation | Production of antibodies using gene libraries |
US5780225A (en) | 1990-01-12 | 1998-07-14 | Stratagene | Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules |
EP1690935A3 (en) | 1990-01-12 | 2008-07-30 | Abgenix, Inc. | Generation of xenogeneic antibodies |
US5427908A (en) | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
EP0542810A1 (en) | 1990-08-02 | 1993-05-26 | B.R. Centre Limited | Methods for the production of proteins with a desired function |
US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
US5770429A (en) | 1990-08-29 | 1998-06-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
DK0546073T3 (da) | 1990-08-29 | 1998-02-02 | Genpharm Int | Frembringelse og anvendelse af transgene, ikke-humane dyr, der er i stand til at danne heterologe antistoffer |
US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
US5698426A (en) | 1990-09-28 | 1997-12-16 | Ixsys, Incorporated | Surface expression libraries of heteromeric receptors |
ATE164395T1 (de) | 1990-12-03 | 1998-04-15 | Genentech Inc | Verfahren zur anreicherung von proteinvarianten mit geänderten bindungseigenschaften |
CA2108147C (en) | 1991-04-10 | 2009-01-06 | Angray Kang | Heterodimeric receptor libraries using phagemids |
GB9112536D0 (en) | 1991-06-11 | 1991-07-31 | Celltech Ltd | Chemical compounds |
GB9120467D0 (en) | 1991-09-26 | 1991-11-06 | Celltech Ltd | Anti-hmfg antibodies and process for their production |
WO1993011236A1 (en) | 1991-12-02 | 1993-06-10 | Medical Research Council | Production of anti-self antibodies from antibody segment repertoires and displayed on phage |
US5733743A (en) | 1992-03-24 | 1998-03-31 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
US5454806A (en) | 1992-11-06 | 1995-10-03 | Terumo Kabushiki Kaisha | Medical device |
EP0733070A1 (en) | 1993-12-08 | 1996-09-25 | Genzyme Corporation | Process for generating specific antibodies |
DK0744958T3 (da) | 1994-01-31 | 2003-10-20 | Univ Boston | Polyklonale antistofbiblioteker |
FR2716640B1 (fr) | 1994-02-28 | 1996-05-03 | Procedes Machines Speciales | Dispositif de centrage et de blocage d'une pièce en vue de son rodage à l'aide d'un rodoir à expansion. |
US5516637A (en) | 1994-06-10 | 1996-05-14 | Dade International Inc. | Method involving display of protein binding pairs on the surface of bacterial pili and bacteriophage |
US5685727A (en) | 1995-01-20 | 1997-11-11 | Ocean Design, Inc. | Underwater mateable connector |
JP2978435B2 (ja) | 1996-01-24 | 1999-11-15 | チッソ株式会社 | アクリロキシプロピルシランの製造方法 |
US5980898A (en) | 1996-11-14 | 1999-11-09 | The United States Of America As Represented By The U.S. Army Medical Research & Material Command | Adjuvant for transcutaneous immunization |
GB9625640D0 (en) | 1996-12-10 | 1997-01-29 | Celltech Therapeutics Ltd | Biological products |
CN1224712C (zh) * | 1997-06-04 | 2005-10-26 | 牛津生物医学(英国)有限公司 | 载体 |
DK1049787T3 (da) | 1998-01-23 | 2005-04-04 | Vlaams Interuniv Inst Biotech | Antistofderivater med flere anvendelsesmuligheder |
GB9812545D0 (en) | 1998-06-10 | 1998-08-05 | Celltech Therapeutics Ltd | Biological products |
WO2000002809A1 (en) | 1998-07-10 | 2000-01-20 | East/West Industries, Inc. | Floor crane with rotatable boom |
US20060228364A1 (en) | 1999-12-24 | 2006-10-12 | Genentech, Inc. | Serum albumin binding peptides for tumor targeting |
US6908963B2 (en) | 2001-10-09 | 2005-06-21 | Nektar Therapeutics Al, Corporation | Thioester polymer derivatives and method of modifying the N-terminus of a polypeptide therewith |
ES2374068T3 (es) | 2002-12-03 | 2012-02-13 | Ucb Pharma, S.A. | Ensayo para identificar células productoras de anticuerpos. |
GB0312481D0 (en) | 2003-05-30 | 2003-07-09 | Celltech R&D Ltd | Antibodies |
GB0315450D0 (en) | 2003-07-01 | 2003-08-06 | Celltech R&D Ltd | Biological products |
GB0412181D0 (en) | 2004-06-01 | 2004-06-30 | Celltech R&D Ltd | Biological products |
KR101336204B1 (ko) | 2006-08-18 | 2013-12-03 | 주식회사 케이티 | 다시점 비디오에서 전역변이를 이용하여 상이한 시점의 화면들을 압축 또는 복호하는 인코더와 인코딩하는 방법 및디코더와 디코딩하는 방법 |
GB0619291D0 (en) | 2006-09-29 | 2006-11-08 | Ucb Sa | Altered antibodies |
AU2008282218A1 (en) | 2007-07-31 | 2009-02-05 | Medimmune, Llc | Multispecific epitope binding proteins and uses thereof |
ES2628395T3 (es) | 2007-08-15 | 2017-08-02 | Bayer Pharma Aktiengesellschaft | Anticuerpo regulado por proteasa |
CN101842387B (zh) | 2007-09-26 | 2014-05-07 | Ucb医药有限公司 | 双特异性抗体融合物 |
WO2010035012A1 (en) | 2008-09-26 | 2010-04-01 | Ucb Pharma S.A. | Biological products |
KR20110124369A (ko) | 2009-04-07 | 2011-11-16 | 로슈 글리카트 아게 | 이중특이적 항erbb3/항cmet 항체 |
PT2417156E (pt) | 2009-04-07 | 2015-04-29 | Roche Glycart Ag | Anticorpos trivalentes, biespecíficos |
AU2010252284A1 (en) * | 2009-05-27 | 2011-11-17 | F. Hoffmann-La Roche Ag | Tri- or tetraspecific antibodies |
CN102597298B (zh) | 2009-09-09 | 2014-09-17 | 耳蜗有限公司 | 具有通过多个涂层形成的基本上连续的阻挡层的绝缘传导元件 |
ES2667258T3 (es) * | 2009-09-10 | 2018-05-10 | Ucb Biopharma Sprl | Anticuerpos multivalentes |
GB201005063D0 (en) * | 2010-03-25 | 2010-05-12 | Ucb Pharma Sa | Biological products |
GB0920127D0 (en) * | 2009-11-17 | 2009-12-30 | Ucb Pharma Sa | Antibodies |
JP5758004B2 (ja) * | 2010-08-24 | 2015-08-05 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | ジスルフィドによって安定化されたFv断片を含む二重特異性抗体 |
MX340556B (es) | 2010-08-24 | 2016-07-14 | Roche Glycart Ag | Anticuerpos biespecificos activables. |
MX348738B (es) * | 2011-07-13 | 2017-06-27 | Ucb Pharma Sa | Cepa huesped bacteriana que expresa dsbc recombinante. |
SI2776466T1 (sl) * | 2011-11-11 | 2017-12-29 | Ucb Biopharma Sprl | Protitelesa, ki se vežejo na albumin in njihovi vezavni fragmenti |
UA112203C2 (uk) * | 2011-11-11 | 2016-08-10 | Юсб Фарма С.А. | Злитий білок біоспецифічного антитіла, який зв'язується з ox40 людини та сироватковим альбуміном людини |
GB201223276D0 (en) * | 2012-12-21 | 2013-02-06 | Ucb Pharma Sa | Antibodies and methods of producing same |
GB201506869D0 (en) * | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
GB201506870D0 (en) * | 2015-04-22 | 2015-06-03 | Ucb Biopharma Sprl | Method |
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