RU2014135538A - Способ изготовления микрочипа для реакции амплификации нуклеиновых кислот - Google Patents
Способ изготовления микрочипа для реакции амплификации нуклеиновых кислот Download PDFInfo
- Publication number
- RU2014135538A RU2014135538A RU2014135538A RU2014135538A RU2014135538A RU 2014135538 A RU2014135538 A RU 2014135538A RU 2014135538 A RU2014135538 A RU 2014135538A RU 2014135538 A RU2014135538 A RU 2014135538A RU 2014135538 A RU2014135538 A RU 2014135538A
- Authority
- RU
- Russia
- Prior art keywords
- reagent
- solution
- nucleic acid
- amplification reaction
- microchip
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 17
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 17
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 17
- 238000004519 manufacturing process Methods 0.000 title claims abstract 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract 24
- 230000003321 amplification Effects 0.000 claims abstract 16
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract 16
- 238000001035 drying Methods 0.000 claims abstract 9
- 230000008014 freezing Effects 0.000 claims abstract 6
- 238000007710 freezing Methods 0.000 claims abstract 6
- 230000004807 localization Effects 0.000 claims abstract 5
- 102000004190 Enzymes Human genes 0.000 claims abstract 4
- 108090000790 Enzymes Proteins 0.000 claims abstract 4
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract 4
- 239000000203 mixture Substances 0.000 claims abstract 2
- 239000000126 substance Substances 0.000 claims abstract 2
- 239000013615 primer Substances 0.000 claims 2
- -1 - curing step Chemical class 0.000 claims 1
- 239000003155 DNA primer Substances 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000001291 vacuum drying Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Dispersion Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2012052322 | 2012-03-08 | ||
| JP2012-052322 | 2012-03-08 | ||
| PCT/JP2013/050652 WO2013132891A1 (ja) | 2012-03-08 | 2013-01-16 | 核酸増幅反応用マイクロチップの製造方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2014135538A true RU2014135538A (ru) | 2016-03-20 |
Family
ID=49116378
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014135538A RU2014135538A (ru) | 2012-03-08 | 2013-01-16 | Способ изготовления микрочипа для реакции амплификации нуклеиновых кислот |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9545630B2 (en:Method) |
| EP (1) | EP2824172B1 (en:Method) |
| JP (1) | JP5987895B2 (en:Method) |
| KR (1) | KR20140143139A (en:Method) |
| CN (1) | CN104160011A (en:Method) |
| IN (1) | IN2014MN01628A (en:Method) |
| RU (1) | RU2014135538A (en:Method) |
| WO (1) | WO2013132891A1 (en:Method) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019513235A (ja) * | 2016-03-15 | 2019-05-23 | アボット モレキュラー インク． | 自動分析のためのシステム及び方法 |
| EP3556853B1 (en) * | 2016-12-13 | 2021-09-22 | Eiken Kagaku Kabushiki Kaisha | Microchip |
| CN110885902B (zh) * | 2018-09-10 | 2022-10-21 | 北京亿森宝生物科技有限公司 | 用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的冻干微芯片、试剂盒及方法 |
| CN110885899B (zh) * | 2018-09-10 | 2023-04-14 | 中国动物疫病预防控制中心（农业部屠宰技术中心） | 用于鉴别16种禽病病原冻干微芯片、试剂盒及方法 |
| WO2025094815A1 (ja) * | 2023-10-30 | 2025-05-08 | Ｔｏｐｐａｎホールディングス株式会社 | 流体デバイス |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0775518B2 (ja) * | 1989-09-29 | 1995-08-16 | 勝 大鶴 | ▲高▼血圧、▲高▼脂血症、肥満の予防及び治療に有効な飲食用キノコタンパク質及びその抽出方法 |
| US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
| JPH08291078A (ja) * | 1995-04-21 | 1996-11-05 | M I O:Kk | 高血圧及び高脂血症の予防・治療に有効で且つ抗腫瘍作用を有する飲食用キノコタンパク質、及び肥満の予防・治療に有効で且つ抗腫瘍作用を有する飲食用キノコタンパク質、並びにそれらの抽出方法 |
| JPH10234822A (ja) * | 1997-02-28 | 1998-09-08 | Material Eng Tech Lab Inc | 凍結乾燥用容器 |
| EP1099387A4 (en) * | 1999-05-19 | 2005-07-06 | Inc Admin Agency Naro | METHOD AND DEVICE FOR LYOPHILIZATION AND LYOPHILIZED MATERIAL |
| US6875619B2 (en) * | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
| ES2180416B1 (es) * | 2001-03-12 | 2004-06-01 | BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. | Procedimiento para la preparacion de mezclas de reaccion estabilizadas, total o parcialmente desecadas, que comprenden, al menos, una enzima, mezclas de reaccion y kits que las contienen. |
| GB0414815D0 (en) * | 2004-07-02 | 2004-08-04 | Secr Defence | Method for stabilising reagents which are useful for nucleic acid amplification |
| CN100460013C (zh) * | 2006-09-05 | 2009-02-11 | 重庆康卫生物科技有限公司 | 口服重组幽门螺杆菌疫苗及其制备方法 |
| AU2009296528A1 (en) * | 2008-09-24 | 2010-04-01 | Straus Holdings Inc. | Imaging analyzer for testing analytes |
| AU2009331083B2 (en) * | 2008-12-25 | 2016-02-11 | Universal Bio Research Co., Ltd. | Method for pretreating specimen and method for assaying biological substance |
| EP2391452B1 (en) * | 2009-01-30 | 2015-06-17 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
| JP5691187B2 (ja) * | 2010-02-10 | 2015-04-01 | ソニー株式会社 | 核酸増幅反応用マイクロチップ及びその製造方法 |
| JP5786295B2 (ja) * | 2010-06-22 | 2015-09-30 | ソニー株式会社 | 核酸等温増幅反応用マイクロチップ及びその製造方法並びに核酸等温増幅方法 |
| JP2012080870A (ja) * | 2010-09-16 | 2012-04-26 | Sony Corp | 核酸定量方法及び核酸増幅反応用マイクロチップ |
-
2013
- 2013-01-16 US US14/378,588 patent/US9545630B2/en active Active
- 2013-01-16 IN IN1628MUN2014 patent/IN2014MN01628A/en unknown
- 2013-01-16 JP JP2014503511A patent/JP5987895B2/ja active Active
- 2013-01-16 WO PCT/JP2013/050652 patent/WO2013132891A1/ja active Application Filing
- 2013-01-16 KR KR1020147023987A patent/KR20140143139A/ko not_active Withdrawn
- 2013-01-16 RU RU2014135538A patent/RU2014135538A/ru not_active Application Discontinuation
- 2013-01-16 EP EP13758540.2A patent/EP2824172B1/en not_active Not-in-force
- 2013-01-16 CN CN201380012088.2A patent/CN104160011A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2824172A4 (en) | 2015-11-04 |
| JPWO2013132891A1 (ja) | 2015-07-30 |
| US9545630B2 (en) | 2017-01-17 |
| WO2013132891A1 (ja) | 2013-09-12 |
| EP2824172A1 (en) | 2015-01-14 |
| KR20140143139A (ko) | 2014-12-15 |
| US20150017318A1 (en) | 2015-01-15 |
| EP2824172B1 (en) | 2017-09-06 |
| IN2014MN01628A (en:Method) | 2015-05-15 |
| CN104160011A (zh) | 2014-11-19 |
| JP5987895B2 (ja) | 2016-09-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA94 | Acknowledgement of application withdrawn (non-payment of fees) |
Effective date: 20170213 |