ES2721204T3 - Circularización y amplificación de ácidos nucleicos circulantes asistida por ligasa - Google Patents
Circularización y amplificación de ácidos nucleicos circulantes asistida por ligasa Download PDFInfo
- Publication number
- ES2721204T3 ES2721204T3 ES14750119T ES14750119T ES2721204T3 ES 2721204 T3 ES2721204 T3 ES 2721204T3 ES 14750119 T ES14750119 T ES 14750119T ES 14750119 T ES14750119 T ES 14750119T ES 2721204 T3 ES2721204 T3 ES 2721204T3
- Authority
- ES
- Spain
- Prior art keywords
- dna
- ligase
- circularization
- assisted
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/307—Circular oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/137—Concentration of a component of medium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Un método para generar un círculo de ADN monocatenario a partir de un ADN lineal, comprendiendo el método: (a) proporcionar el ADN lineal; (b) incubar el ADN lineal con una polinucleótido cinasa en presencia de un donador de fosfato para generar una secuencia de ADN ligable que tiene un grupo fosfato en un extremo 5' terminal y un grupo hidroxilo en un extremo 3' terminal; y (c) incubar la secuencia de ADN ligable con una ligasa que es capaz de ligación intramolecular independiente de la plantilla de una secuencia de ADN monocatenario para generar el círculo de ADN monocatenario, donde todas las etapas del método se llevan a cabo en un único recipiente de reacción sin intervención de ninguna etapa de aislamiento o purificación y donde la etapa (b) se lleva a cabo usando un donador de fosfato distinto de adenosina trifosfato o desoxiadenosina trifosfato.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/952,040 US9217167B2 (en) | 2013-07-26 | 2013-07-26 | Ligase-assisted nucleic acid circularization and amplification |
| PCT/US2014/048179 WO2015013604A1 (en) | 2013-07-26 | 2014-07-25 | Ligase-assisted nucleic acid circularization and amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2721204T3 true ES2721204T3 (es) | 2019-07-29 |
Family
ID=51300893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14750119T Active ES2721204T3 (es) | 2013-07-26 | 2014-07-25 | Circularización y amplificación de ácidos nucleicos circulantes asistida por ligasa |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9217167B2 (es) |
| EP (1) | EP3024945B1 (es) |
| JP (2) | JP6574178B2 (es) |
| KR (2) | KR102435352B1 (es) |
| CN (1) | CN105392901B (es) |
| AU (1) | AU2014292947B2 (es) |
| CA (1) | CA2917452C (es) |
| ES (1) | ES2721204T3 (es) |
| IL (1) | IL243672B (es) |
| MX (1) | MX366661B (es) |
| SG (1) | SG11201600582QA (es) |
| WO (1) | WO2015013604A1 (es) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8583380B2 (en) | 2008-09-05 | 2013-11-12 | Aueon, Inc. | Methods for stratifying and annotating cancer drug treatment options |
| EP2619329B1 (en) | 2010-09-24 | 2019-05-22 | The Board of Trustees of The Leland Stanford Junior University | Direct capture, amplification and sequencing of target dna using immobilized primers |
| US10655167B2 (en) | 2013-07-26 | 2020-05-19 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
| US10767222B2 (en) | 2013-12-11 | 2020-09-08 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
| US11859246B2 (en) * | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US10335078B2 (en) | 2014-08-04 | 2019-07-02 | General Electric Company | Device for separation and collection of plasma |
| CN115161178A (zh) | 2015-09-09 | 2022-10-11 | 集联健康有限公司 | 用于样品收集、稳定化和保存的系统、方法和装置 |
| CA2999902A1 (en) | 2015-10-09 | 2017-04-13 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| ES2964592T3 (es) * | 2015-11-05 | 2024-04-08 | Global Life Sciences Solutions Operations UK Ltd | Circularización y amplificación de ácido nucleico asistida por ligasa |
| US20190062827A1 (en) * | 2016-03-14 | 2019-02-28 | RGENE, Inc. | HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES |
| EP3458586B1 (en) | 2016-05-16 | 2022-12-28 | Accuragen Holdings Limited | Method of improved sequencing by strand identification |
| JP2019517252A (ja) | 2016-05-31 | 2019-06-24 | クアンタポール， インコーポレイテッド | 二色ナノポア配列決定 |
| CN107557355A (zh) * | 2016-07-01 | 2018-01-09 | Pgi股份有限公司 | 建构环状模板和检测dna分子的方法 |
| CA3033749A1 (en) | 2016-08-15 | 2018-02-22 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
| KR102366854B1 (ko) | 2016-09-06 | 2022-02-22 | 이화여자대학교 산학협력단 | 회전환 증폭 기반 분자 진단 방법 |
| US11091791B2 (en) | 2017-02-24 | 2021-08-17 | Mgi Tech Co., Ltd. | Methods for hybridization based hook ligation |
| WO2019090621A1 (zh) | 2017-11-09 | 2019-05-16 | 深圳华大智造科技有限公司 | 钩状探针、核酸连接方法以及测序文库的构建方法 |
| US11203782B2 (en) | 2018-03-29 | 2021-12-21 | Accuragen Holdings Limited | Compositions and methods comprising asymmetric barcoding |
| CN109161572B (zh) * | 2018-07-05 | 2021-11-12 | 中国海洋大学 | 单链环状核酸及其制备方法和应用 |
| CN112359090A (zh) * | 2018-08-07 | 2021-02-12 | 深圳市真迈生物科技有限公司 | 一种核酸片段的连接方法、测序文库的构建方法及其应用 |
| US11195518B2 (en) | 2019-03-27 | 2021-12-07 | Sonova Ag | Hearing device user communicating with a wireless communication device |
| CN111321143B (zh) * | 2020-02-16 | 2021-09-17 | 中国海洋大学 | 一种制备环状rna的方法 |
| CN111718980A (zh) * | 2020-05-14 | 2020-09-29 | 中国地质大学（武汉） | 一种编辑识别特定核酸链的检测方法 |
| WO2022015461A1 (en) | 2020-07-17 | 2022-01-20 | Qiagen Beverly Llc | Thermostable ligase with reduced sequence bias |
| CN114836401A (zh) * | 2022-04-24 | 2022-08-02 | 中国人民解放军海军军医大学 | 重组ssDNA核酸环化酶、制备方法及应用 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7993839B2 (en) * | 2001-01-19 | 2011-08-09 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| GB2378245A (en) | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| US6977153B2 (en) * | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| WO2006037102A2 (en) * | 2004-09-27 | 2006-04-06 | Epicentre Technologies | Methods for identifying polymerase inhibitors |
| WO2007140417A2 (en) | 2006-05-31 | 2007-12-06 | Sequenom, Inc. | Methods and compositions for the extraction and amplification of nucleic acid from a sample |
| JP2008109874A (ja) * | 2006-10-30 | 2008-05-15 | Toyobo Co Ltd | Ｄｎａの自己環状化用酵素組成物 |
| US20080242560A1 (en) | 2006-11-21 | 2008-10-02 | Gunderson Kevin L | Methods for generating amplified nucleic acid arrays |
| US8003330B2 (en) | 2007-09-28 | 2011-08-23 | Pacific Biosciences Of California, Inc. | Error-free amplification of DNA for clonal sequencing |
| PT2963709T (pt) * | 2008-10-24 | 2017-08-21 | Epicentre Tech Corp | Composições de extremidade de transposão e métodos de modificação de ácidos nucleicos |
| EP2396430B1 (en) | 2009-02-16 | 2013-05-01 | Epicentre Technologies Corporation | Template-independent ligation of single-stranded dna |
| JP2012080871A (ja) * | 2009-12-14 | 2012-04-26 | National Agriculture & Food Research Organization | Ｒｎａの直接検出法 |
| ES2626411T3 (es) | 2010-03-10 | 2017-07-25 | Ibis Biosciences, Inc. | Producción de ácido nucleico circular monocatenario |
| WO2011123749A1 (en) * | 2010-04-01 | 2011-10-06 | New England Biolabs, Inc. | Compositions and methods for adenylating oligonucleotides |
| US9487807B2 (en) | 2010-12-27 | 2016-11-08 | Ibis Biosciences, Inc. | Compositions and methods for producing single-stranded circular DNA |
-
2013
- 2013-07-26 US US13/952,040 patent/US9217167B2/en active Active
-
2014
- 2014-07-25 CN CN201480042259.0A patent/CN105392901B/zh active Active
- 2014-07-25 AU AU2014292947A patent/AU2014292947B2/en active Active
- 2014-07-25 MX MX2016001157A patent/MX366661B/es active IP Right Grant
- 2014-07-25 ES ES14750119T patent/ES2721204T3/es active Active
- 2014-07-25 JP JP2016530068A patent/JP6574178B2/ja active Active
- 2014-07-25 SG SG11201600582QA patent/SG11201600582QA/en unknown
- 2014-07-25 WO PCT/US2014/048179 patent/WO2015013604A1/en active Application Filing
- 2014-07-25 CA CA2917452A patent/CA2917452C/en active Active
- 2014-07-25 KR KR1020167001763A patent/KR102435352B1/ko active IP Right Grant
- 2014-07-25 EP EP14750119.1A patent/EP3024945B1/en active Active
- 2014-07-25 KR KR1020217006992A patent/KR20210029845A/ko not_active Application Discontinuation
-
2016
- 2016-01-19 IL IL24367216A patent/IL243672B/en active IP Right Grant
-
2019
- 2019-02-28 JP JP2019035123A patent/JP2019107015A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20150031086A1 (en) | 2015-01-29 |
| US9217167B2 (en) | 2015-12-22 |
| CN105392901A (zh) | 2016-03-09 |
| IL243672A0 (en) | 2016-03-31 |
| CN105392901B (zh) | 2021-02-02 |
| MX366661B (es) | 2019-07-18 |
| AU2014292947A1 (en) | 2016-01-28 |
| JP6574178B2 (ja) | 2019-09-11 |
| CA2917452C (en) | 2022-12-06 |
| JP2016525360A (ja) | 2016-08-25 |
| IL243672B (en) | 2019-11-28 |
| KR20210029845A (ko) | 2021-03-16 |
| KR102435352B1 (ko) | 2022-08-23 |
| JP2019107015A (ja) | 2019-07-04 |
| CA2917452A1 (en) | 2015-01-29 |
| AU2014292947B2 (en) | 2020-06-18 |
| EP3024945B1 (en) | 2019-03-27 |
| SG11201600582QA (en) | 2016-02-26 |
| EP3024945A1 (en) | 2016-06-01 |
| KR20160034305A (ko) | 2016-03-29 |
| WO2015013604A1 (en) | 2015-01-29 |
| MX2016001157A (es) | 2016-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2721204T3 (es) | Circularización y amplificación de ácidos nucleicos circulantes asistida por ligasa | |
| CY1125029T1 (el) | Τροποποιημενοι νουκλεοζιτες, νουκλεοτιδια, και νουκλεϊκα οξεα και χρησεις αυτων | |
| CO2017011957A2 (es) | Variantes de alfa-amilasa y polinucleótidos que codifican las mismas | |
| ES2610461T3 (es) | Método de detección de nucleótidos únicos | |
| MX2020003841A (es) | Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos. | |
| MX365323B (es) | Amplificación de ácidos nucléicos. | |
| WO2015196130A3 (en) | Alternative nucleic acid molecules and uses thereof | |
| EA201792538A1 (ru) | Способ биологического получения метакриловой кислоты | |
| EP3656873A3 (en) | Polynucleotide enrichment and amplification using argonaute systems | |
| CL2016003324A1 (es) | Construcciones de anticuerpos multiespecificos. | |
| BR112018010118A2 (pt) | inibidores de cxcr2 | |
| EA201101141A1 (ru) | Получение закрытой линейной днк | |
| BR112017008082A2 (pt) | composições e métodos para detectar um vírus de rna | |
| AR099462A1 (es) | Unidad de ultrafiltración para el intercambio continuo de medio o tampón de una solución proteica | |
| BR112017013691A2 (pt) | composição compreendendo um polímero em multiestágio, seu método de preparação e seu uso | |
| AU2013228077B2 (en) | Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction | |
| EP4269582A3 (en) | Target enrichment by unidirectional dual probe primer extension | |
| CL2016003066A1 (es) | Fertilizante de desmontaje basado en biomasa explotada | |
| DOP2022000085A (es) | Composiciones y métodos para inhibir la expresión del gen alas1 | |
| BR112017020513A2 (pt) | método de produção de peixe para transplante, peixe para transplante, método de produção de espécie híbrida de peixe e espécie híbrida de peixe | |
| CO2017009163A2 (es) | Moléculas de ácido nucleico de arn polimerasa ii33 para el control de plagas de insectos | |
| BR112018011209A2 (pt) | processo de cultura de algas vermelhas unicelulares (uras) com permeado lácteo | |
| WO2014210416A8 (en) | Helicase suppression of non-template amplification | |
| MX370616B (es) | Amplificacion de acidos nucleicos. | |
| BR112016030604B8 (pt) | Processo para preparação de ácidos 3- hidroxipicolínicos |