SG11201600582QA - Ligase-assisted nucleic acid circularization and amplification - Google Patents

Ligase-assisted nucleic acid circularization and amplification

Info

Publication number
SG11201600582QA
SG11201600582QA SG11201600582QA SG11201600582QA SG11201600582QA SG 11201600582Q A SG11201600582Q A SG 11201600582QA SG 11201600582Q A SG11201600582Q A SG 11201600582QA SG 11201600582Q A SG11201600582Q A SG 11201600582QA SG 11201600582Q A SG11201600582Q A SG 11201600582QA
Authority
SG
Singapore
Prior art keywords
ligase
amplification
nucleic acid
assisted nucleic
circularization
Prior art date
Application number
SG11201600582QA
Other languages
English (en)
Inventor
Ryan Charles Heller
John Richard Nelson
Erik Leeming Kvam
Original Assignee
Gen Electric
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric filed Critical Gen Electric
Publication of SG11201600582QA publication Critical patent/SG11201600582QA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/501Ligase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30Oligonucleotides characterised by their secondary structure
    • C12Q2525/307Circular oligonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/137Concentration of a component of medium

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11201600582QA 2013-07-26 2014-07-25 Ligase-assisted nucleic acid circularization and amplification SG11201600582QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/952,040 US9217167B2 (en) 2013-07-26 2013-07-26 Ligase-assisted nucleic acid circularization and amplification
PCT/US2014/048179 WO2015013604A1 (en) 2013-07-26 2014-07-25 Ligase-assisted nucleic acid circularization and amplification

Publications (1)

Publication Number Publication Date
SG11201600582QA true SG11201600582QA (en) 2016-02-26

Family

ID=51300893

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201600582QA SG11201600582QA (en) 2013-07-26 2014-07-25 Ligase-assisted nucleic acid circularization and amplification

Country Status (12)

Country Link
US (1) US9217167B2 (es)
EP (1) EP3024945B1 (es)
JP (2) JP6574178B2 (es)
KR (2) KR102435352B1 (es)
CN (1) CN105392901B (es)
AU (1) AU2014292947B2 (es)
CA (1) CA2917452C (es)
ES (1) ES2721204T3 (es)
IL (1) IL243672B (es)
MX (1) MX366661B (es)
SG (1) SG11201600582QA (es)
WO (1) WO2015013604A1 (es)

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US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
EP2619329B1 (en) 2010-09-24 2019-05-22 The Board of Trustees of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US10655167B2 (en) 2013-07-26 2020-05-19 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11859246B2 (en) * 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US10335078B2 (en) 2014-08-04 2019-07-02 General Electric Company Device for separation and collection of plasma
CN115161178A (zh) 2015-09-09 2022-10-11 集联健康有限公司 用于样品收集、稳定化和保存的系统、方法和装置
CA2999902A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
ES2964592T3 (es) * 2015-11-05 2024-04-08 Global Life Sciences Solutions Operations UK Ltd Circularización y amplificación de ácido nucleico asistida por ligasa
US20190062827A1 (en) * 2016-03-14 2019-02-28 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
JP2019517252A (ja) 2016-05-31 2019-06-24 クアンタポール, インコーポレイテッド 二色ナノポア配列決定
CN107557355A (zh) * 2016-07-01 2018-01-09 Pgi股份有限公司 建构环状模板和检测dna分子的方法
CA3033749A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
KR102366854B1 (ko) 2016-09-06 2022-02-22 이화여자대학교 산학협력단 회전환 증폭 기반 분자 진단 방법
US11091791B2 (en) 2017-02-24 2021-08-17 Mgi Tech Co., Ltd. Methods for hybridization based hook ligation
WO2019090621A1 (zh) 2017-11-09 2019-05-16 深圳华大智造科技有限公司 钩状探针、核酸连接方法以及测序文库的构建方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
CN109161572B (zh) * 2018-07-05 2021-11-12 中国海洋大学 单链环状核酸及其制备方法和应用
CN112359090A (zh) * 2018-08-07 2021-02-12 深圳市真迈生物科技有限公司 一种核酸片段的连接方法、测序文库的构建方法及其应用
US11195518B2 (en) 2019-03-27 2021-12-07 Sonova Ag Hearing device user communicating with a wireless communication device
CN111321143B (zh) * 2020-02-16 2021-09-17 中国海洋大学 一种制备环状rna的方法
CN111718980A (zh) * 2020-05-14 2020-09-29 中国地质大学(武汉) 一种编辑识别特定核酸链的检测方法
WO2022015461A1 (en) 2020-07-17 2022-01-20 Qiagen Beverly Llc Thermostable ligase with reduced sequence bias
CN114836401A (zh) * 2022-04-24 2022-08-02 中国人民解放军海军军医大学 重组ssDNA核酸环化酶、制备方法及应用

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US7993839B2 (en) * 2001-01-19 2011-08-09 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
GB2378245A (en) 2001-08-03 2003-02-05 Mats Nilsson Nucleic acid amplification method
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
WO2006037102A2 (en) * 2004-09-27 2006-04-06 Epicentre Technologies Methods for identifying polymerase inhibitors
WO2007140417A2 (en) 2006-05-31 2007-12-06 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
JP2008109874A (ja) * 2006-10-30 2008-05-15 Toyobo Co Ltd Dnaの自己環状化用酵素組成物
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US8003330B2 (en) 2007-09-28 2011-08-23 Pacific Biosciences Of California, Inc. Error-free amplification of DNA for clonal sequencing
PT2963709T (pt) * 2008-10-24 2017-08-21 Epicentre Tech Corp Composições de extremidade de transposão e métodos de modificação de ácidos nucleicos
EP2396430B1 (en) 2009-02-16 2013-05-01 Epicentre Technologies Corporation Template-independent ligation of single-stranded dna
JP2012080871A (ja) * 2009-12-14 2012-04-26 National Agriculture & Food Research Organization Rnaの直接検出法
ES2626411T3 (es) 2010-03-10 2017-07-25 Ibis Biosciences, Inc. Producción de ácido nucleico circular monocatenario
WO2011123749A1 (en) * 2010-04-01 2011-10-06 New England Biolabs, Inc. Compositions and methods for adenylating oligonucleotides
US9487807B2 (en) 2010-12-27 2016-11-08 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular DNA

Also Published As

Publication number Publication date
US20150031086A1 (en) 2015-01-29
US9217167B2 (en) 2015-12-22
CN105392901A (zh) 2016-03-09
IL243672A0 (en) 2016-03-31
CN105392901B (zh) 2021-02-02
MX366661B (es) 2019-07-18
AU2014292947A1 (en) 2016-01-28
JP6574178B2 (ja) 2019-09-11
CA2917452C (en) 2022-12-06
JP2016525360A (ja) 2016-08-25
IL243672B (en) 2019-11-28
ES2721204T3 (es) 2019-07-29
KR20210029845A (ko) 2021-03-16
KR102435352B1 (ko) 2022-08-23
JP2019107015A (ja) 2019-07-04
CA2917452A1 (en) 2015-01-29
AU2014292947B2 (en) 2020-06-18
EP3024945B1 (en) 2019-03-27
EP3024945A1 (en) 2016-06-01
KR20160034305A (ko) 2016-03-29
WO2015013604A1 (en) 2015-01-29
MX2016001157A (es) 2016-08-08

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