IN2014MN01628A - - Google Patents
Download PDFInfo
- Publication number
- IN2014MN01628A IN2014MN01628A IN1628MUN2014A IN2014MN01628A IN 2014MN01628 A IN2014MN01628 A IN 2014MN01628A IN 1628MUN2014 A IN1628MUN2014 A IN 1628MUN2014A IN 2014MN01628 A IN2014MN01628 A IN 2014MN01628A
- Authority
- IN
- India
- Prior art keywords
- nucleic acid
- acid amplification
- amplification reaction
- microchip
- reaction
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
Abstract
Provided is a method for producing a microchip for use in a nucleic acid amplification reaction which enables a simple and highly accurate analysis. Provided is a method for producing a microchip for use in a nucleic acid amplification reaction comprising: a solidification step of drying a reagent solution containing at least a portion of a substance needed for the nucleic acid amplification reaction; and an accommodation step of placing a reagent containing the solidified substance in a well which is a reaction place for the nucleic acid amplification reaction. In the microchip for use in a nucleic acid amplification reaction which is produced by the production method the substance needed for the nucleic acid amplification reaction is accommodated in a solidified state. Therefore it becomes possible to inhibit the occurrence of a non specific amplification in a nucleic acid amplification reaction and therefore it becomes possible to carry out an analysis with high accuracy.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012052322 | 2012-03-08 | ||
PCT/JP2013/050652 WO2013132891A1 (en) | 2012-03-08 | 2013-01-16 | Method for producing microchip for use in nucleic acid amplification reaction |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2014MN01628A true IN2014MN01628A (en) | 2015-05-15 |
Family
ID=49116378
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN1628MUN2014 IN2014MN01628A (en) | 2012-03-08 | 2013-01-16 |
Country Status (8)
Country | Link |
---|---|
US (1) | US9545630B2 (en) |
EP (1) | EP2824172B1 (en) |
JP (1) | JP5987895B2 (en) |
KR (1) | KR20140143139A (en) |
CN (1) | CN104160011A (en) |
IN (1) | IN2014MN01628A (en) |
RU (1) | RU2014135538A (en) |
WO (1) | WO2013132891A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109477849B (en) * | 2016-03-15 | 2022-10-21 | 雅培分子公司 | Automatic analysis system and method |
WO2018109829A1 (en) * | 2016-12-13 | 2018-06-21 | 栄研化学株式会社 | Microchip |
CN110885899B (en) * | 2018-09-10 | 2023-04-14 | 中国动物疫病预防控制中心(农业部屠宰技术中心) | Freeze-drying microchip, kit and method for identifying 16 avian disease pathogens |
CN110885902B (en) * | 2018-09-10 | 2022-10-21 | 北京亿森宝生物科技有限公司 | Freeze-drying microchip, kit and method for detecting porcine reproductive and respiratory syndrome virus and identifying highly pathogenic classical variant strain of porcine reproductive and respiratory syndrome virus |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0775518B2 (en) | 1989-09-29 | 1995-08-16 | 勝 大鶴 | Mushroom protein for eating and drinking, which is effective in preventing and treating hypertension, hyperlipidemia and obesity, and its extraction method |
US5556771A (en) * | 1995-02-10 | 1996-09-17 | Gen-Probe Incorporated | Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification |
JPH08291078A (en) | 1995-04-21 | 1996-11-05 | M I O:Kk | Mushroom protein for food and beverage effective in preventing and treating hypertension and hyperlipemia and having antitumor action, mushroom protein for food and beverage effective in preventing and treating obesity and having antitumor action and method for extracting these proteins |
JPH10234822A (en) | 1997-02-28 | 1998-09-08 | Material Eng Tech Lab Inc | Container for lyophilization |
JP3626986B2 (en) | 1999-05-19 | 2005-03-09 | 有限会社つくば食料科学研究所 | Freeze-drying method, apparatus and freeze-dried product |
US6875619B2 (en) * | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
ES2180416B1 (en) * | 2001-03-12 | 2004-06-01 | BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. | PROCEDURE FOR THE PREPARATION OF STABILIZED REACTION MIXTURES, TOTAL OR PARTIALLY DESIRED, THAT INCLUDE, AT LEAST, ONE ENZYME, REACTION MIXES AND KITS CONTAINING THEM. |
GB0414815D0 (en) * | 2004-07-02 | 2004-08-04 | Secr Defence | Method for stabilising reagents which are useful for nucleic acid amplification |
CN100460013C (en) | 2006-09-05 | 2009-02-11 | 重庆康卫生物科技有限公司 | Oral administration recombinant helicobacterpylori vaccine and preparing method thereof |
US10384203B2 (en) | 2008-09-24 | 2019-08-20 | First Light Biosciences, Inc. | Kits and devices for detecting analytes |
US9182395B2 (en) * | 2008-12-25 | 2015-11-10 | Universal Bio Research Co., Ltd. | Method for pretreating specimen and method for assaying biological substance |
EP2391452B1 (en) * | 2009-01-30 | 2015-06-17 | Gen-Probe Incorporated | Systems and methods for detecting a signal and applying thermal energy to a signal transmission element |
JP5691187B2 (en) | 2010-02-10 | 2015-04-01 | ソニー株式会社 | Microchip for nucleic acid amplification reaction and method for producing the same |
JP5786295B2 (en) | 2010-06-22 | 2015-09-30 | ソニー株式会社 | Nucleic acid isothermal amplification microchip, method for producing the same, and nucleic acid isothermal amplification method |
JP2012080870A (en) | 2010-09-16 | 2012-04-26 | Sony Corp | Method for quantifying nucleic acid and microchip for nucleic acid amplification reaction |
-
2013
- 2013-01-16 US US14/378,588 patent/US9545630B2/en active Active
- 2013-01-16 WO PCT/JP2013/050652 patent/WO2013132891A1/en active Application Filing
- 2013-01-16 EP EP13758540.2A patent/EP2824172B1/en active Active
- 2013-01-16 RU RU2014135538A patent/RU2014135538A/en not_active Application Discontinuation
- 2013-01-16 CN CN201380012088.2A patent/CN104160011A/en active Pending
- 2013-01-16 IN IN1628MUN2014 patent/IN2014MN01628A/en unknown
- 2013-01-16 JP JP2014503511A patent/JP5987895B2/en active Active
- 2013-01-16 KR KR1020147023987A patent/KR20140143139A/en not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP2824172A4 (en) | 2015-11-04 |
US9545630B2 (en) | 2017-01-17 |
RU2014135538A (en) | 2016-03-20 |
KR20140143139A (en) | 2014-12-15 |
JP5987895B2 (en) | 2016-09-07 |
EP2824172A1 (en) | 2015-01-14 |
CN104160011A (en) | 2014-11-19 |
WO2013132891A1 (en) | 2013-09-12 |
EP2824172B1 (en) | 2017-09-06 |
JPWO2013132891A1 (en) | 2015-07-30 |
US20150017318A1 (en) | 2015-01-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
SG11201605344YA (en) | Analysis of nucleic acids associated with single cells using nucleic acid barcodes | |
HK1220494A1 (en) | Method of generating a three-dimensional nucleic acid containing matrix | |
WO2012129157A3 (en) | System for performing polymerase chain reaction nucleic acid amplification | |
IL234864B (en) | Integrated optoelectronic read and fluidic cartridge useful for nucleic acid sequencing | |
EP2821486A4 (en) | Method of introducing nucleic acid into plant cells | |
EP3003560A4 (en) | Cartridge for preparing a sample fluid containing cells for analysis | |
GB201205769D0 (en) | Methods for preparing samples for nucleic acid amplification | |
EP3054285A4 (en) | Biochip for raman quantitative analysis of biological samples | |
GB201219137D0 (en) | Direct nucleic acid amplification kit, reagent and method | |
IN2014MN01628A (en) | ||
MX342218B (en) | Analytical aid. | |
TN2013000369A1 (en) | Preventing or reducing scale in wet-process phosphoric acid production | |
MX2015013310A (en) | Method and reagent for determining vitamin d metabolites. | |
EP2837696A4 (en) | Method for preparing a nucleic acid with high precision used in detecting a nucleic acid with a nucleic acid polymerase | |
EP2789692A4 (en) | Method for measuring cells, and reagent for cell measurement | |
EP2722388A4 (en) | Nucleic acid probe for assaying nucleic acids | |
MX356270B (en) | Methods for decontaminating circuits for producing glucose polymers and hydrolysates of glucose polymers. | |
EP2832862A4 (en) | Method for measuring substance in blood sample | |
EP2990789A4 (en) | Method for analysis for organic substance contained in solution to be examined | |
EP2717054A4 (en) | Method for inhibiting non-specific reaction in pivka-ii measurement reagent | |
GB201317680D0 (en) | Representation of spatial-frequency data as a map | |
HK1217537A1 (en) | System and method for measuring an analyte in a sample and calculating hematocrit-insensitive glucose concentrations | |
WO2013140140A3 (en) | Electrochemical temperature measurement and sensor | |
EP2878673A4 (en) | Preparation method and preparation device for sample for nucleic acid amplification reaction | |
IN2014CN02937A (en) |