RU2013113407A - Способы улавливания и секвенирования нуклеиновых кислот - Google Patents
Способы улавливания и секвенирования нуклеиновых кислот Download PDFInfo
- Publication number
- RU2013113407A RU2013113407A RU2013113407/10A RU2013113407A RU2013113407A RU 2013113407 A RU2013113407 A RU 2013113407A RU 2013113407/10 A RU2013113407/10 A RU 2013113407/10A RU 2013113407 A RU2013113407 A RU 2013113407A RU 2013113407 A RU2013113407 A RU 2013113407A
- Authority
- RU
- Russia
- Prior art keywords
- nucleic acid
- polymerase
- genomic dna
- solid support
- primer
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 36
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 30
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 30
- 238000000034 method Methods 0.000 title claims abstract 25
- 108020004414 DNA Proteins 0.000 claims abstract 16
- 239000007787 solid Substances 0.000 claims abstract 12
- 239000002773 nucleotide Substances 0.000 claims abstract 9
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 9
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims abstract 7
- 238000006116 polymerization reaction Methods 0.000 claims abstract 7
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract 5
- 238000009396 hybridization Methods 0.000 claims abstract 4
- 229910019142 PO4 Inorganic materials 0.000 claims abstract 3
- 235000021317 phosphate Nutrition 0.000 claims abstract 3
- 150000003013 phosphoric acid derivatives Chemical group 0.000 claims abstract 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims abstract 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims abstract 2
- 102100034343 Integrase Human genes 0.000 claims abstract 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims abstract 2
- 230000000694 effects Effects 0.000 claims abstract 2
- 239000000203 mixture Substances 0.000 claims abstract 2
- 238000012163 sequencing technique Methods 0.000 claims abstract 2
- 239000012634 fragment Substances 0.000 claims 12
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 2
- 239000002105 nanoparticle Substances 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US40235010P | 2010-08-27 | 2010-08-27 | |
| US61/402,350 | 2010-08-27 | ||
| PCT/US2011/049151 WO2012027572A2 (en) | 2010-08-27 | 2011-08-25 | Methods for nucleic acid capture and sequencing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2013113407A true RU2013113407A (ru) | 2014-10-10 |
Family
ID=44545975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2013113407/10A RU2013113407A (ru) | 2010-08-27 | 2011-08-25 | Способы улавливания и секвенирования нуклеиновых кислот |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20130324419A1 (enExample) |
| EP (1) | EP2609214A2 (enExample) |
| JP (1) | JP2013535986A (enExample) |
| KR (1) | KR20130101031A (enExample) |
| CN (1) | CN103080338A (enExample) |
| BR (1) | BR112013002299A2 (enExample) |
| CA (1) | CA2803693A1 (enExample) |
| MX (1) | MX2013001799A (enExample) |
| RU (1) | RU2013113407A (enExample) |
| WO (1) | WO2012027572A2 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| WO2012106546A2 (en) | 2011-02-02 | 2012-08-09 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| NO2694769T3 (enExample) | 2012-03-06 | 2018-03-03 | ||
| EP2875173B1 (en) * | 2012-07-17 | 2017-06-28 | Counsyl, Inc. | System and methods for detecting genetic variation |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| EP2970951B1 (en) | 2013-03-13 | 2019-02-20 | Illumina, Inc. | Methods for nucleic acid sequencing |
| AU2014364926B2 (en) | 2013-12-20 | 2017-12-14 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic DNA samples |
| KR102472027B1 (ko) * | 2014-10-17 | 2022-11-30 | 일루미나 케임브리지 리미티드 | 근접 보존 전위 |
| PT3207134T (pt) * | 2014-10-17 | 2019-09-17 | Illumina Cambridge Ltd | Transposição que preserva a contiguidade |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| EP3235010A4 (en) | 2014-12-18 | 2018-08-29 | Agilome, Inc. | Chemically-sensitive field effect transistor |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10395759B2 (en) * | 2015-05-18 | 2019-08-27 | Regeneron Pharmaceuticals, Inc. | Methods and systems for copy number variant detection |
| WO2017201081A1 (en) | 2016-05-16 | 2017-11-23 | Agilome, Inc. | Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids |
| EP3650553B1 (en) * | 2018-11-07 | 2023-07-12 | Siemens Healthcare GmbH | Method for detection of specific nucleic acids |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO165894C (no) * | 1988-05-24 | 1991-04-24 | Gunnar Paulsen | Analysemetode for gener. |
| US6982146B1 (en) * | 1999-08-30 | 2006-01-03 | The United States Of America As Represented By The Department Of Health And Human Services | High speed parallel molecular nucleic acid sequencing |
| WO2008115185A2 (en) | 2006-04-24 | 2008-09-25 | Nimblegen Systems, Inc. | Use of microarrays for genomic representation selection |
| WO2008096146A1 (en) * | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
| WO2008103848A1 (en) * | 2007-02-21 | 2008-08-28 | Invitrogen Corporation | Materials and methods for single molecule nucleic acid sequencing |
| WO2009024019A1 (en) * | 2007-08-15 | 2009-02-26 | The University Of Hong Kong | Methods and compositions for high-throughput bisulphite dna-sequencing and utilities |
| US20110281740A1 (en) | 2008-06-30 | 2011-11-17 | Joseph Beechem | Methods for Real Time Single Molecule Sequencing |
| US8795961B2 (en) * | 2008-09-05 | 2014-08-05 | Pacific Biosciences Of California, Inc. | Preparations, compositions, and methods for nucleic acid sequencing |
| WO2010085343A1 (en) | 2009-01-23 | 2010-07-29 | Cold Spring Harbor Laboratory | Methods and arrays for profiling dna methylation |
-
2011
- 2011-08-25 RU RU2013113407/10A patent/RU2013113407A/ru not_active Application Discontinuation
- 2011-08-25 MX MX2013001799A patent/MX2013001799A/es not_active Application Discontinuation
- 2011-08-25 BR BR112013002299A patent/BR112013002299A2/pt not_active IP Right Cessation
- 2011-08-25 EP EP11752056.9A patent/EP2609214A2/en not_active Withdrawn
- 2011-08-25 CA CA2803693A patent/CA2803693A1/en not_active Abandoned
- 2011-08-25 JP JP2013526153A patent/JP2013535986A/ja active Pending
- 2011-08-25 KR KR1020137007586A patent/KR20130101031A/ko not_active Withdrawn
- 2011-08-25 US US13/819,657 patent/US20130324419A1/en not_active Abandoned
- 2011-08-25 CN CN2011800416596A patent/CN103080338A/zh active Pending
- 2011-08-25 WO PCT/US2011/049151 patent/WO2012027572A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| BR112013002299A2 (pt) | 2016-05-24 |
| CN103080338A (zh) | 2013-05-01 |
| MX2013001799A (es) | 2013-05-20 |
| WO2012027572A3 (en) | 2012-06-07 |
| EP2609214A2 (en) | 2013-07-03 |
| KR20130101031A (ko) | 2013-09-12 |
| US20130324419A1 (en) | 2013-12-05 |
| JP2013535986A (ja) | 2013-09-19 |
| WO2012027572A2 (en) | 2012-03-01 |
| CA2803693A1 (en) | 2012-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2013113407A (ru) | Способы улавливания и секвенирования нуклеиновых кислот | |
| JP5986572B2 (ja) | 固定化プライマーを使用した標的ｄｎａの直接的な捕捉、増幅、および配列決定 | |
| US9944924B2 (en) | Polynucleotide modification on solid support | |
| US8329394B2 (en) | Methods and substances for isolation and detection of small polynucleotides | |
| US20140296094A1 (en) | Systems and methods for detection of genomic copy number changes | |
| CN103806111A (zh) | 高通量测序文库的构建方法及其应用 | |
| JP2019076100A (ja) | 多重メチル化特異的増幅システムおよび方法 | |
| CN101067156A (zh) | 一种基于选择性探针的多重pcr方法及其应用 | |
| JP2016537990A (ja) | 核酸プローブ及びゲノム断片検出方法 | |
| US20100273164A1 (en) | Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation | |
| JP2016539624A5 (enExample) | ||
| CA2888953A1 (en) | Method for the simultaneous amplification of a plurality of different nucleic acid target sequences | |
| RU2014126423A (ru) | Высокопроизводительный анализ однонуклеотидных полиморфизмов | |
| GB2612412A (en) | Systems and methods for targeted nucleic acid capture | |
| WO2012083481A1 (zh) | 用于hpa基因分型的方法及所用引物 | |
| JPWO2021087275A5 (enExample) | ||
| CN107109478B (zh) | 获得配对末端测序信息的方法 | |
| WO2008093336A3 (en) | Genome determination assay | |
| ES2421459B1 (es) | Cebadores especiales para la reacción en cadena de la polimerasa | |
| Qiu et al. | Mitochondrial single nucleotide polymorphism genotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using cleavable biotinylated dideoxynucleotides | |
| RU2014107505A (ru) | Генетический маркер для диагностики деменции с тельцами леви | |
| RU2006134100A (ru) | Способ проведения аллель-специфичной пцр для генотипирования крупного рогатого скота по аллелям а и в гена каппа-казеина | |
| RU2014113962A (ru) | Набор олигонуклеотидных праймеров и зондов для генотипирования полиморфных локусов днк, ассоциированных с риском развития спорадической формы болезни альцгеймера в российских популяциях | |
| RU2015151954A (ru) | Способ предсказания рецидива рака желудка | |
| Reinders | Amplification of bisulfite-converted DNA for genome-wide DNA methylation profiling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20160608 |