CN103080338A - 核酸捕获和测序方法 - Google Patents

核酸捕获和测序方法 Download PDF

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Publication number
CN103080338A
CN103080338A CN2011800416596A CN201180041659A CN103080338A CN 103080338 A CN103080338 A CN 103080338A CN 2011800416596 A CN2011800416596 A CN 2011800416596A CN 201180041659 A CN201180041659 A CN 201180041659A CN 103080338 A CN103080338 A CN 103080338A
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China
Prior art keywords
nucleic acid
genomic dna
solid support
target nucleic
dna fragment
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CN2011800416596A
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English (en)
Chinese (zh)
Inventor
S·塞沙基里
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication date
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Publication of CN103080338A publication Critical patent/CN103080338A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800416596A 2010-08-27 2011-08-25 核酸捕获和测序方法 Pending CN103080338A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40235010P 2010-08-27 2010-08-27
US61/402,350 2010-08-27
PCT/US2011/049151 WO2012027572A2 (en) 2010-08-27 2011-08-25 Methods for nucleic acid capture and sequencing

Publications (1)

Publication Number Publication Date
CN103080338A true CN103080338A (zh) 2013-05-01

Family

ID=44545975

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800416596A Pending CN103080338A (zh) 2010-08-27 2011-08-25 核酸捕获和测序方法

Country Status (10)

Country Link
US (1) US20130324419A1 (enExample)
EP (1) EP2609214A2 (enExample)
JP (1) JP2013535986A (enExample)
KR (1) KR20130101031A (enExample)
CN (1) CN103080338A (enExample)
BR (1) BR112013002299A2 (enExample)
CA (1) CA2803693A1 (enExample)
MX (1) MX2013001799A (enExample)
RU (1) RU2013113407A (enExample)
WO (1) WO2012027572A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113166795A (zh) * 2018-11-07 2021-07-23 西门子医疗有限公司 用于检测特定核酸的方法

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US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
NO2694769T3 (enExample) 2012-03-06 2018-03-03
WO2014015084A2 (en) * 2012-07-17 2014-01-23 Counsyl, Inc. System and methods for detecting genetic variation
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
CN106414765A (zh) 2013-12-20 2017-02-15 Illumina公司 在片段化的基因组dna样品中保留基因组连接信息
EP3636757A1 (en) * 2014-10-17 2020-04-15 Illumina Cambridge Limited Contiguity preserving transposition
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10395759B2 (en) * 2015-05-18 2019-08-27 Regeneron Pharmaceuticals, Inc. Methods and systems for copy number variant detection
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis

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NO165894C (no) * 1988-05-24 1991-04-24 Gunnar Paulsen Analysemetode for gener.
WO2008115185A2 (en) 2006-04-24 2008-09-25 Nimblegen Systems, Inc. Use of microarrays for genomic representation selection
EP2126072A4 (en) * 2007-02-21 2011-07-13 Life Technologies Corp MATERIALS AND METHOD FOR THE INDIVIDUAL MOLECULE SEQUENCING OF NUCLEIC ACIDS
WO2009024019A1 (en) * 2007-08-15 2009-02-26 The University Of Hong Kong Methods and compositions for high-throughput bisulphite dna-sequencing and utilities
US20110281740A1 (en) 2008-06-30 2011-11-17 Joseph Beechem Methods for Real Time Single Molecule Sequencing
WO2010027497A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
US20120149593A1 (en) 2009-01-23 2012-06-14 Hicks James B Methods and arrays for profiling dna methylation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
詹绍康等: "《实用医学统计方法》", 30 November 1999, 上海科学技术文献出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113166795A (zh) * 2018-11-07 2021-07-23 西门子医疗有限公司 用于检测特定核酸的方法

Also Published As

Publication number Publication date
EP2609214A2 (en) 2013-07-03
KR20130101031A (ko) 2013-09-12
US20130324419A1 (en) 2013-12-05
CA2803693A1 (en) 2012-03-01
RU2013113407A (ru) 2014-10-10
WO2012027572A3 (en) 2012-06-07
BR112013002299A2 (pt) 2016-05-24
MX2013001799A (es) 2013-05-20
JP2013535986A (ja) 2013-09-19
WO2012027572A2 (en) 2012-03-01

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