CA2803693A1 - Methods for nucleic acid capture and sequencing - Google Patents

Methods for nucleic acid capture and sequencing Download PDF

Info

Publication number
CA2803693A1
CA2803693A1 CA2803693A CA2803693A CA2803693A1 CA 2803693 A1 CA2803693 A1 CA 2803693A1 CA 2803693 A CA2803693 A CA 2803693A CA 2803693 A CA2803693 A CA 2803693A CA 2803693 A1 CA2803693 A1 CA 2803693A1
Authority
CA
Canada
Prior art keywords
nucleic acid
genomic dna
polymerase
dna fragment
solid support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2803693A
Other languages
English (en)
French (fr)
Inventor
Somasekar Seshagiri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of CA2803693A1 publication Critical patent/CA2803693A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2803693A 2010-08-27 2011-08-25 Methods for nucleic acid capture and sequencing Abandoned CA2803693A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40235010P 2010-08-27 2010-08-27
US61/402,350 2010-08-27
PCT/US2011/049151 WO2012027572A2 (en) 2010-08-27 2011-08-25 Methods for nucleic acid capture and sequencing

Publications (1)

Publication Number Publication Date
CA2803693A1 true CA2803693A1 (en) 2012-03-01

Family

ID=44545975

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2803693A Abandoned CA2803693A1 (en) 2010-08-27 2011-08-25 Methods for nucleic acid capture and sequencing

Country Status (10)

Country Link
US (1) US20130324419A1 (enExample)
EP (1) EP2609214A2 (enExample)
JP (1) JP2013535986A (enExample)
KR (1) KR20130101031A (enExample)
CN (1) CN103080338A (enExample)
BR (1) BR112013002299A2 (enExample)
CA (1) CA2803693A1 (enExample)
MX (1) MX2013001799A (enExample)
RU (1) RU2013113407A (enExample)
WO (1) WO2012027572A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104160040A (zh) * 2012-03-06 2014-11-19 伊鲁米纳剑桥有限公司 核酸测序的改进方法
US12680124B2 (en) 2018-11-07 2026-07-14 Siemens Healthineers Ag Method for detection of specific nucleic acids

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
AU2013292610B2 (en) * 2012-07-17 2018-05-17 Myriad Women's Health, Inc. System and methods for detecting genetic variation
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
BR122021026779B1 (pt) 2014-10-17 2023-12-19 Illumina Cambridge Limited Transposon de preservação de contiguidade
CN114045282B (zh) * 2014-10-17 2025-03-07 伊卢米纳剑桥有限公司 接近性保留性转座
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10429342B2 (en) 2014-12-18 2019-10-01 Edico Genome Corporation Chemically-sensitive field effect transistor
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10395759B2 (en) * 2015-05-18 2019-08-27 Regeneron Pharmaceuticals, Inc. Methods and systems for copy number variant detection
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
WO2026162398A1 (en) * 2025-01-31 2026-08-06 Medicover Genetics Ltd. Method of detecting methylation sites in genomic dna

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO165894C (no) * 1988-05-24 1991-04-24 Gunnar Paulsen Analysemetode for gener.
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
EP2010657A2 (en) 2006-04-24 2009-01-07 Nimblegen Systems, Inc. Use of microarrays for genomic representation selection
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
WO2008103848A1 (en) * 2007-02-21 2008-08-28 Invitrogen Corporation Materials and methods for single molecule nucleic acid sequencing
WO2009024019A1 (en) * 2007-08-15 2009-02-26 The University Of Hong Kong Methods and compositions for high-throughput bisulphite dna-sequencing and utilities
WO2010002939A2 (en) 2008-06-30 2010-01-07 Life Technologies Corporation Methods for real time single molecule sequencing
US8795961B2 (en) * 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
WO2010085343A1 (en) 2009-01-23 2010-07-29 Cold Spring Harbor Laboratory Methods and arrays for profiling dna methylation

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104160040A (zh) * 2012-03-06 2014-11-19 伊鲁米纳剑桥有限公司 核酸测序的改进方法
US9574226B2 (en) 2012-03-06 2017-02-21 Illumina, Inc. Methods of nucleic acid sequencing
CN104160040B (zh) * 2012-03-06 2017-07-07 伊鲁米纳剑桥有限公司 核酸测序的改进方法
US10267804B2 (en) 2012-03-06 2019-04-23 Illumina Cambridge Limited Methods of nucleic acid sequencing
US11834699B2 (en) 2012-03-06 2023-12-05 Illumina Cambridge Limited Methods of nucleic acid sequencing
US12680124B2 (en) 2018-11-07 2026-07-14 Siemens Healthineers Ag Method for detection of specific nucleic acids

Also Published As

Publication number Publication date
KR20130101031A (ko) 2013-09-12
WO2012027572A2 (en) 2012-03-01
MX2013001799A (es) 2013-05-20
CN103080338A (zh) 2013-05-01
BR112013002299A2 (pt) 2016-05-24
WO2012027572A3 (en) 2012-06-07
US20130324419A1 (en) 2013-12-05
RU2013113407A (ru) 2014-10-10
JP2013535986A (ja) 2013-09-19
EP2609214A2 (en) 2013-07-03

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Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20170825