RU2012122166A - Способы получения рекомбинантных белков с увеличенными эффективностями секреции - Google Patents
Способы получения рекомбинантных белков с увеличенными эффективностями секреции Download PDFInfo
- Publication number
- RU2012122166A RU2012122166A RU2012122166/10A RU2012122166A RU2012122166A RU 2012122166 A RU2012122166 A RU 2012122166A RU 2012122166/10 A RU2012122166/10 A RU 2012122166/10A RU 2012122166 A RU2012122166 A RU 2012122166A RU 2012122166 A RU2012122166 A RU 2012122166A
- Authority
- RU
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- Prior art keywords
- cell
- protein
- vps10
- activity
- vacuolar sorting
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25637909P | 2009-10-30 | 2009-10-30 | |
| US61/256,379 | 2009-10-30 | ||
| US35066810P | 2010-06-02 | 2010-06-02 | |
| US61/350,668 | 2010-06-02 | ||
| PCT/US2010/053903 WO2011053541A1 (en) | 2009-10-30 | 2010-10-25 | Methods for the production of recombinant proteins with improved secretion efficiencies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012122166A true RU2012122166A (ru) | 2013-12-10 |
Family
ID=43922476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012122166/10A RU2012122166A (ru) | 2009-10-30 | 2010-10-25 | Способы получения рекомбинантных белков с увеличенными эффективностями секреции |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20130011875A1 (enExample) |
| EP (1) | EP2494053A4 (enExample) |
| JP (1) | JP2013509180A (enExample) |
| KR (1) | KR20140015137A (enExample) |
| CN (1) | CN102686731A (enExample) |
| AU (1) | AU2010313608A1 (enExample) |
| BR (1) | BR112012009886A2 (enExample) |
| CA (1) | CA2777487A1 (enExample) |
| IN (1) | IN2012DN03823A (enExample) |
| MX (1) | MX2012004993A (enExample) |
| RU (1) | RU2012122166A (enExample) |
| WO (1) | WO2011053541A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120213728A1 (en) * | 2009-10-30 | 2012-08-23 | Merck | Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris |
| WO2012124567A1 (ja) * | 2011-03-11 | 2012-09-20 | 株式会社カネカ | Vps遺伝子が破壊されている酵母を用いる異種タンパク質の製造方法 |
| EP2800809B1 (en) | 2012-01-05 | 2018-03-07 | Glykos Finland Oy | Protease deficient filamentous fungal cells and methods of use thereof |
| CN102559740B (zh) * | 2012-02-25 | 2013-03-27 | 山东大学 | 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株 |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| CA2904035A1 (en) * | 2013-03-06 | 2014-09-12 | Protalix Ltd. | Tnf alpha inhibitor polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same |
| DK3019602T3 (en) | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| AU2015293949B2 (en) | 2014-07-21 | 2019-07-25 | Teknologian Tutkimuskeskus Vtt Oy | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi |
| EA038931B9 (ru) * | 2014-11-20 | 2022-02-18 | Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. | Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений |
| US10752907B2 (en) * | 2015-08-05 | 2020-08-25 | Lonza Ltd | Promoter variants |
| US10988515B2 (en) | 2017-01-13 | 2021-04-27 | Bolt Threads, Inc. | Elastomeric proteins |
| EP4286524A3 (en) | 2017-07-31 | 2024-03-06 | Melt&Marble AB | Fungal cell with improved protein production capacity |
| SE2051317A1 (en) * | 2020-11-11 | 2022-05-12 | Nielsen Dina Petranovic | A genetically modified yeast cell |
| CN113604373A (zh) * | 2021-02-08 | 2021-11-05 | 江南大学 | 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株 |
| CN113736818A (zh) * | 2021-10-08 | 2021-12-03 | 江南大学 | 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法 |
| CN113862242B (zh) * | 2021-10-15 | 2024-06-25 | 江南大学 | 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法 |
| EP4194560A1 (en) | 2021-12-08 | 2023-06-14 | European Molecular Biology Laboratory | Improved production of secreted proteins in fungal cells |
| CN115851468A (zh) * | 2022-07-18 | 2023-03-28 | 江南大学 | 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用 |
| CN116478905B (zh) * | 2023-02-15 | 2025-07-22 | 北京中医药大学 | 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用 |
| WO2025000334A1 (zh) * | 2023-06-29 | 2025-01-02 | 中国科学院深圳先进技术研究院 | 融合蛋白及其在制备代谢产物中的应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| ATE438722T1 (de) | 2000-05-17 | 2009-08-15 | Mitsubishi Tanabe Pharma Corp | Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| US7598055B2 (en) | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| US8697394B2 (en) | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| ATE440959T1 (de) | 2000-06-28 | 2009-09-15 | Glycofi Inc | Verfahren für die herstellung modifizierter glykoproteine |
| US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| GB0121088D0 (en) | 2001-08-31 | 2001-10-24 | Cambridge Consultants | Electric kettle |
| EP2359685B1 (en) | 2001-12-27 | 2013-12-04 | GlycoFi, Inc. | Methods to engineer mammalian-type carbohydrate structures |
| US7252933B2 (en) * | 2002-06-26 | 2007-08-07 | Flanders Interuniversity Institute For Biotechnology | Protein glycosylation modification in methylotrophic yeast |
| AU2003291448A1 (en) | 2002-11-12 | 2004-06-03 | Purdue Research Foundation | Benzoate inducible promoters |
| CA2551484C (en) | 2003-12-24 | 2015-03-31 | Glycofi, Inc. | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
| US7479389B2 (en) | 2004-03-02 | 2009-01-20 | Glycofi, Inc. | ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration |
| WO2005090552A2 (en) | 2004-03-17 | 2005-09-29 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
| CN101084233B (zh) | 2004-04-29 | 2012-08-15 | 格利科菲公司 | 用于在糖蛋白产生中减少或消除α-甘露糖苷酶抗性聚糖的方法 |
| CA2606253A1 (en) | 2005-04-21 | 2006-10-26 | Uti Limited Partnership | Pcr for mrsa sccmec typing |
| BRPI0711898A2 (pt) | 2006-05-19 | 2011-12-27 | Glycofi Inc | composiÇço de proteÍna da eritropoietina substancialmente homogÊnea, composiÇço farmacÊutica, e, mÉtodos de aumentar hematàcrito em um mamÍfero, e de produzir uma composiÇço de eritropoietina |
| AU2007254141B2 (en) * | 2006-05-19 | 2012-10-11 | Glycofi, Inc. | Erythropoietin compositions |
-
2010
- 2010-10-25 AU AU2010313608A patent/AU2010313608A1/en not_active Abandoned
- 2010-10-25 RU RU2012122166/10A patent/RU2012122166A/ru not_active Application Discontinuation
- 2010-10-25 WO PCT/US2010/053903 patent/WO2011053541A1/en not_active Ceased
- 2010-10-25 US US13/503,707 patent/US20130011875A1/en not_active Abandoned
- 2010-10-25 MX MX2012004993A patent/MX2012004993A/es not_active Application Discontinuation
- 2010-10-25 CN CN2010800519511A patent/CN102686731A/zh active Pending
- 2010-10-25 KR KR1020127010917A patent/KR20140015137A/ko not_active Withdrawn
- 2010-10-25 EP EP20100827360 patent/EP2494053A4/en not_active Withdrawn
- 2010-10-25 IN IN3823DEN2012 patent/IN2012DN03823A/en unknown
- 2010-10-25 CA CA2777487A patent/CA2777487A1/en not_active Abandoned
- 2010-10-25 JP JP2012536916A patent/JP2013509180A/ja not_active Withdrawn
- 2010-10-25 BR BR112012009886A patent/BR112012009886A2/pt not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| BR112012009886A2 (pt) | 2015-09-15 |
| MX2012004993A (es) | 2012-06-12 |
| EP2494053A4 (en) | 2013-10-30 |
| CN102686731A (zh) | 2012-09-19 |
| KR20140015137A (ko) | 2014-02-06 |
| WO2011053541A1 (en) | 2011-05-05 |
| CA2777487A1 (en) | 2011-05-05 |
| AU2010313608A1 (en) | 2012-06-07 |
| EP2494053A1 (en) | 2012-09-05 |
| US20130011875A1 (en) | 2013-01-10 |
| JP2013509180A (ja) | 2013-03-14 |
| IN2012DN03823A (enExample) | 2015-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA93 | Acknowledgement of application withdrawn (no request for examination) |
Effective date: 20131028 |