RU2012122166A - Способы получения рекомбинантных белков с увеличенными эффективностями секреции - Google Patents
Способы получения рекомбинантных белков с увеличенными эффективностями секреции Download PDFInfo
- Publication number
- RU2012122166A RU2012122166A RU2012122166/10A RU2012122166A RU2012122166A RU 2012122166 A RU2012122166 A RU 2012122166A RU 2012122166/10 A RU2012122166/10 A RU 2012122166/10A RU 2012122166 A RU2012122166 A RU 2012122166A RU 2012122166 A RU2012122166 A RU 2012122166A
- Authority
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- Prior art keywords
- cell
- protein
- vps10
- activity
- vacuolar sorting
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims abstract 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims abstract 4
- 238000000034 method Methods 0.000 title claims 13
- 230000028327 secretion Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 claims abstract 35
- 230000000694 effects Effects 0.000 claims abstract 22
- 102000004169 proteins and genes Human genes 0.000 claims abstract 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract 11
- 230000037430 deletion Effects 0.000 claims abstract 11
- 238000012217 deletion Methods 0.000 claims abstract 11
- 230000002538 fungal effect Effects 0.000 claims abstract 7
- 102000003886 Glycoproteins Human genes 0.000 claims abstract 6
- 108090000288 Glycoproteins Proteins 0.000 claims abstract 6
- 239000013604 expression vector Substances 0.000 claims abstract 6
- 239000003112 inhibitor Substances 0.000 claims abstract 4
- 230000008030 elimination Effects 0.000 claims abstract 3
- 238000003379 elimination reaction Methods 0.000 claims abstract 3
- 230000013595 glycosylation Effects 0.000 claims abstract 3
- 238000006206 glycosylation reaction Methods 0.000 claims abstract 3
- 238000004519 manufacturing process Methods 0.000 claims abstract 2
- 230000035772 mutation Effects 0.000 claims abstract 2
- 108020004707 nucleic acids Proteins 0.000 claims abstract 2
- 102000039446 nucleic acids Human genes 0.000 claims abstract 2
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 2
- 239000002773 nucleotide Substances 0.000 claims abstract 2
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 2
- 229940121649 protein inhibitor Drugs 0.000 claims abstract 2
- 239000012268 protein inhibitor Substances 0.000 claims abstract 2
- 239000000126 substance Substances 0.000 claims abstract 2
- 210000004027 cell Anatomy 0.000 claims 33
- 241000235058 Komagataella pastoris Species 0.000 claims 12
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 9
- 241000235648 Pichia Species 0.000 claims 5
- -1 Vps38 Proteins 0.000 claims 4
- 241000233866 Fungi Species 0.000 claims 3
- 101150080255 PEP1 gene Proteins 0.000 claims 3
- 101150037257 VPS10 gene Proteins 0.000 claims 3
- 238000012258 culturing Methods 0.000 claims 2
- 239000001963 growth medium Substances 0.000 claims 2
- 238000002955 isolation Methods 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 230000001131 transforming effect Effects 0.000 claims 2
- 241000228245 Aspergillus niger Species 0.000 claims 1
- 101100049521 Caenorhabditis elegans vps-11 gene Proteins 0.000 claims 1
- 101100156534 Caenorhabditis elegans vps-18 gene Proteins 0.000 claims 1
- 101100102810 Caenorhabditis elegans vps-39 gene Proteins 0.000 claims 1
- 101100540621 Caenorhabditis elegans vps-53 gene Proteins 0.000 claims 1
- 241000222122 Candida albicans Species 0.000 claims 1
- 102100026314 Charged multivesicular body protein 6 Human genes 0.000 claims 1
- 241000235036 Debaryomyces hansenii Species 0.000 claims 1
- 102100022557 Hepatocyte growth factor-regulated tyrosine kinase substrate Human genes 0.000 claims 1
- 101001045469 Homo sapiens Hepatocyte growth factor-regulated tyrosine kinase substrate Proteins 0.000 claims 1
- 101000605630 Homo sapiens Phosphatidylinositol 3-kinase catalytic subunit type 3 Proteins 0.000 claims 1
- 101000702653 Homo sapiens Sorting nexin-1 Proteins 0.000 claims 1
- 101000708470 Homo sapiens Sorting nexin-3 Proteins 0.000 claims 1
- 101000868440 Homo sapiens Sorting nexin-8 Proteins 0.000 claims 1
- 101100174574 Mus musculus Pikfyve gene Proteins 0.000 claims 1
- 241000320412 Ogataea angusta Species 0.000 claims 1
- 102100038329 Phosphatidylinositol 3-kinase catalytic subunit type 3 Human genes 0.000 claims 1
- 241000235060 Scheffersomyces stipitis Species 0.000 claims 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 claims 1
- 102100030992 Sorting nexin-1 Human genes 0.000 claims 1
- 102100032829 Sorting nexin-3 Human genes 0.000 claims 1
- 102100032848 Sorting nexin-8 Human genes 0.000 claims 1
- 101150084332 VPS16 gene Proteins 0.000 claims 1
- 101150115214 VPS28 gene Proteins 0.000 claims 1
- 101150051052 VPS29 gene Proteins 0.000 claims 1
- 101150079645 VPS41 gene Proteins 0.000 claims 1
- 101150068211 VPS45 gene Proteins 0.000 claims 1
- 101150118009 VPS51 gene Proteins 0.000 claims 1
- 101150055870 VPS52 gene Proteins 0.000 claims 1
- 101150046315 VPS54 gene Proteins 0.000 claims 1
- 101150108883 VPS8 gene Proteins 0.000 claims 1
- 241000222126 [Candida] glabrata Species 0.000 claims 1
- 229940095731 candida albicans Drugs 0.000 claims 1
- 208000032343 candida glabrata infection Diseases 0.000 claims 1
- 101150085659 chmp3 gene Proteins 0.000 claims 1
- 230000001086 cytosolic effect Effects 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 210000002288 golgi apparatus Anatomy 0.000 claims 1
- 102000005962 receptors Human genes 0.000 claims 1
- 108020003175 receptors Proteins 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 101150002794 vps25 gene Proteins 0.000 claims 1
- 101150069463 vps26a gene Proteins 0.000 claims 1
- 101150090036 vps36 gene Proteins 0.000 claims 1
- 108700005075 Regulator Genes Proteins 0.000 abstract 1
- 230000002103 transcriptional effect Effects 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 238000011144 upstream manufacturing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25637909P | 2009-10-30 | 2009-10-30 | |
| US61/256,379 | 2009-10-30 | ||
| US35066810P | 2010-06-02 | 2010-06-02 | |
| US61/350,668 | 2010-06-02 | ||
| PCT/US2010/053903 WO2011053541A1 (en) | 2009-10-30 | 2010-10-25 | Methods for the production of recombinant proteins with improved secretion efficiencies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012122166A true RU2012122166A (ru) | 2013-12-10 |
Family
ID=43922476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012122166/10A RU2012122166A (ru) | 2009-10-30 | 2010-10-25 | Способы получения рекомбинантных белков с увеличенными эффективностями секреции |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20130011875A1 (enExample) |
| EP (1) | EP2494053A4 (enExample) |
| JP (1) | JP2013509180A (enExample) |
| KR (1) | KR20140015137A (enExample) |
| CN (1) | CN102686731A (enExample) |
| AU (1) | AU2010313608A1 (enExample) |
| BR (1) | BR112012009886A2 (enExample) |
| CA (1) | CA2777487A1 (enExample) |
| IN (1) | IN2012DN03823A (enExample) |
| MX (1) | MX2012004993A (enExample) |
| RU (1) | RU2012122166A (enExample) |
| WO (1) | WO2011053541A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120213728A1 (en) * | 2009-10-30 | 2012-08-23 | Merck | Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris |
| JP5932766B2 (ja) * | 2011-03-11 | 2016-06-08 | 株式会社カネカ | Ｖｐｓ遺伝子が破壊されている酵母を用いる異種タンパク質の製造方法 |
| BR112014016587A2 (pt) | 2012-01-05 | 2020-10-27 | Novartis Ag | células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas |
| CN102559740B (zh) * | 2012-02-25 | 2013-03-27 | 山东大学 | 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株 |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| BR112015021708A2 (pt) * | 2013-03-06 | 2017-08-29 | Protalix Ltd | Inibidor de polipetídeo do tnfa, polinucleotídeo isolado, estrutura de expressão de ácido nucleico, célula de planta, composição farmacêutica e método de produção de um inibidor de polipeptídeo do tnfa |
| DK3019602T3 (en) | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| CA2954974A1 (en) | 2014-07-21 | 2016-01-28 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| EA038931B9 (ru) * | 2014-11-20 | 2022-02-18 | Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. | Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений |
| WO2017021541A1 (en) * | 2015-08-05 | 2017-02-09 | Lonza Ltd | Promoter variants |
| WO2018132821A2 (en) | 2017-01-13 | 2018-07-19 | Bolt Threads, Inc. | Elastomeric proteins |
| US11795487B2 (en) * | 2017-07-31 | 2023-10-24 | Melt & Marble AB | Fungal cell with improved protein production capacity |
| SE2051317A1 (en) * | 2020-11-11 | 2022-05-12 | Nielsen Dina Petranovic | A genetically modified yeast cell |
| CN113604373A (zh) * | 2021-02-08 | 2021-11-05 | 江南大学 | 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株 |
| CN113736818A (zh) * | 2021-10-08 | 2021-12-03 | 江南大学 | 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法 |
| CN113862242B (zh) * | 2021-10-15 | 2024-06-25 | 江南大学 | 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法 |
| EP4194560A1 (en) | 2021-12-08 | 2023-06-14 | European Molecular Biology Laboratory | Improved production of secreted proteins in fungal cells |
| CN115851468A (zh) * | 2022-07-18 | 2023-03-28 | 江南大学 | 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用 |
| CN116478905B (zh) * | 2023-02-15 | 2025-07-22 | 北京中医药大学 | 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用 |
| WO2025000334A1 (zh) * | 2023-06-29 | 2025-01-02 | 中国科学院深圳先进技术研究院 | 融合蛋白及其在制备代谢产物中的应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| DE60139467D1 (de) | 2000-05-17 | 2009-09-17 | Mitsubishi Tanabe Pharma Corp | Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein |
| US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7598055B2 (en) | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| US8697394B2 (en) | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| KR100787073B1 (ko) | 2000-06-28 | 2007-12-21 | 글리코파이, 인크. | 변형된 당단백질의 제조방법 |
| GB0121088D0 (en) * | 2001-08-31 | 2001-10-24 | Cambridge Consultants | Electric kettle |
| KR101001243B1 (ko) | 2001-12-27 | 2010-12-17 | 글리코파이, 인크. | 포유동물-유형 탄수화물 구조의 설계 방법 |
| IL165717A0 (en) * | 2002-06-26 | 2006-01-15 | Flanders Interuniversity Inst | A strain of methylotrophic yeast for producing proteins |
| US7705203B2 (en) | 2002-11-12 | 2010-04-27 | Purdue Research Foundation | Benzoate inductible promoters |
| EP2341128A1 (en) | 2003-12-24 | 2011-07-06 | GlycoFi, Inc. | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
| US7479389B2 (en) | 2004-03-02 | 2009-01-20 | Glycofi, Inc. | ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration |
| CA2558635A1 (en) | 2004-03-17 | 2005-09-29 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
| US7465577B2 (en) | 2004-04-29 | 2008-12-16 | Glycofi, Inc. | Methods for reducing or eliminating α-mannosidase resistant glycans for the production of glycoproteins |
| US20060252069A1 (en) | 2005-04-21 | 2006-11-09 | Zhang Kunyan | Pcr for mrsa sccmec typing |
| CA2651456A1 (en) | 2006-05-19 | 2007-11-29 | Glycofi, Inc. | Recombinant vectors |
| US7851438B2 (en) * | 2006-05-19 | 2010-12-14 | GlycoFi, Incorporated | Erythropoietin compositions |
-
2010
- 2010-10-25 MX MX2012004993A patent/MX2012004993A/es not_active Application Discontinuation
- 2010-10-25 AU AU2010313608A patent/AU2010313608A1/en not_active Abandoned
- 2010-10-25 KR KR1020127010917A patent/KR20140015137A/ko not_active Withdrawn
- 2010-10-25 US US13/503,707 patent/US20130011875A1/en not_active Abandoned
- 2010-10-25 CN CN2010800519511A patent/CN102686731A/zh active Pending
- 2010-10-25 RU RU2012122166/10A patent/RU2012122166A/ru not_active Application Discontinuation
- 2010-10-25 JP JP2012536916A patent/JP2013509180A/ja not_active Withdrawn
- 2010-10-25 IN IN3823DEN2012 patent/IN2012DN03823A/en unknown
- 2010-10-25 EP EP20100827360 patent/EP2494053A4/en not_active Withdrawn
- 2010-10-25 BR BR112012009886A patent/BR112012009886A2/pt not_active IP Right Cessation
- 2010-10-25 WO PCT/US2010/053903 patent/WO2011053541A1/en not_active Ceased
- 2010-10-25 CA CA2777487A patent/CA2777487A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| KR20140015137A (ko) | 2014-02-06 |
| JP2013509180A (ja) | 2013-03-14 |
| US20130011875A1 (en) | 2013-01-10 |
| AU2010313608A1 (en) | 2012-06-07 |
| CA2777487A1 (en) | 2011-05-05 |
| WO2011053541A1 (en) | 2011-05-05 |
| MX2012004993A (es) | 2012-06-12 |
| IN2012DN03823A (enExample) | 2015-08-28 |
| EP2494053A1 (en) | 2012-09-05 |
| EP2494053A4 (en) | 2013-10-30 |
| BR112012009886A2 (pt) | 2015-09-15 |
| CN102686731A (zh) | 2012-09-19 |
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