IN2012DN03823A - - Google Patents
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- Publication number
- IN2012DN03823A IN2012DN03823A IN3823DEN2012A IN2012DN03823A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A IN 3823DEN2012 A IN3823DEN2012 A IN 3823DEN2012A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A
- Authority
- IN
- India
- Prior art keywords
- modified
- vacuolar sorting
- vps10
- sorting activity
- host cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention is related to methods and for producing higher titers of recombinant protein in a modified yeast host cell, for example Pichia pastoris, wherein the modified yeast cell lacks vacuolar sorting activity or has decreased vacuolar sorting activity relative to an unmodified yeast host cell of the same species. In particular embodiments vacuolar sorting activity is reduced or eliminated by deletion or disruption of a gene encoding Vps10 or a Vps10 homolog. The invention is also related to the modified yeast cells which are modified in accordance with the methods disclosed herein.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25637909P | 2009-10-30 | 2009-10-30 | |
US35066810P | 2010-06-02 | 2010-06-02 | |
PCT/US2010/053903 WO2011053541A1 (en) | 2009-10-30 | 2010-10-25 | Methods for the production of recombinant proteins with improved secretion efficiencies |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2012DN03823A true IN2012DN03823A (en) | 2015-08-28 |
Family
ID=43922476
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN3823DEN2012 IN2012DN03823A (en) | 2009-10-30 | 2010-10-25 |
Country Status (12)
Country | Link |
---|---|
US (1) | US20130011875A1 (en) |
EP (1) | EP2494053A4 (en) |
JP (1) | JP2013509180A (en) |
KR (1) | KR20140015137A (en) |
CN (1) | CN102686731A (en) |
AU (1) | AU2010313608A1 (en) |
BR (1) | BR112012009886A2 (en) |
CA (1) | CA2777487A1 (en) |
IN (1) | IN2012DN03823A (en) |
MX (1) | MX2012004993A (en) |
RU (1) | RU2012122166A (en) |
WO (1) | WO2011053541A1 (en) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011053545A1 (en) * | 2009-10-30 | 2011-05-05 | Merck Sharp & Dohme Corp. | Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris |
JP5932766B2 (en) * | 2011-03-11 | 2016-06-08 | 株式会社カネカ | Method for producing heterologous protein using yeast in which VPS gene is disrupted |
RU2645252C2 (en) | 2012-01-05 | 2018-02-19 | Глюкос Финланд Ой | Felamentous fungi cell with protease deficit and methods of its application |
CN102559740B (en) * | 2012-02-25 | 2013-03-27 | 山东大学 | Method for improving secretory expression of heterologous protein by saccharomyces cerevisiae and special saccharomyces cerevisiae strain |
US9695454B2 (en) | 2012-05-23 | 2017-07-04 | Glykos Finland Oy | Production of fucosylated glycoproteins |
BR112015021708A2 (en) | 2013-03-06 | 2017-08-29 | Protalix Ltd | TNFA POLYPEPTIDE INHIBITOR, ISOLATED POLYNUCLEOTIDE, NUCLEIC ACID EXPRESSION STRUCTURE, PLANT CELL, PHARMACEUTICAL COMPOSITION AND METHOD OF PRODUCTION OF A TNFA POLYPEPTIDE INHIBITOR |
CA2916905A1 (en) | 2013-07-10 | 2015-01-15 | Novartis Ag | Multiple proteases deficient filamentous fungal cells and methods of use thereof |
CN108064266A (en) | 2014-07-21 | 2018-05-22 | 格利科斯芬兰公司 | The preparation of the glycoprotein with mammal sample N- glycan in filamentous fungi |
EA038931B9 (en) * | 2014-11-20 | 2022-02-18 | Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. | Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells |
JP2020503882A (en) * | 2017-01-13 | 2020-02-06 | ボルト スレッズ インコーポレイテッド | Elastomer protein |
US11795487B2 (en) * | 2017-07-31 | 2023-10-24 | Melt & Marble AB | Fungal cell with improved protein production capacity |
SE2051317A1 (en) * | 2020-11-11 | 2022-05-12 | Nielsen Dina Petranovic | A genetically modified yeast cell |
CN113604373A (en) * | 2021-02-08 | 2021-11-05 | 江南大学 | Pichia pastoris defective strain for improving yield and enzyme activity of human lysozyme |
CN113736818A (en) * | 2021-10-08 | 2021-12-03 | 江南大学 | Method for improving secretion efficiency and enzyme activity of human lysozyme in pichia pastoris |
CN113862242A (en) * | 2021-10-15 | 2021-12-31 | 江南大学 | Method for weakening vacuole separation and improving yield of trichoderma reesei cellulase |
EP4194560A1 (en) | 2021-12-08 | 2023-06-14 | European Molecular Biology Laboratory | Improved production of secreted proteins in fungal cells |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
US7198921B2 (en) | 2000-05-17 | 2007-04-03 | Mitsubishi Pharma Corporation | Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby |
WO2002000879A2 (en) | 2000-06-28 | 2002-01-03 | Glycofi, Inc. | Methods for producing modified glycoproteins |
US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
US7598055B2 (en) | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
US8697394B2 (en) | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
GB0121088D0 (en) * | 2001-08-31 | 2001-10-24 | Cambridge Consultants | Electric kettle |
EP1467615B2 (en) | 2001-12-27 | 2017-03-22 | GlycoFi, Inc. | Methods to engineer mammalian-type carbohydrate structures |
US7252933B2 (en) * | 2002-06-26 | 2007-08-07 | Flanders Interuniversity Institute For Biotechnology | Protein glycosylation modification in methylotrophic yeast |
US7705203B2 (en) | 2002-11-12 | 2010-04-27 | Purdue Research Foundation | Benzoate inductible promoters |
AU2004311545B2 (en) | 2003-12-24 | 2008-11-06 | Glycofi, Inc. | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
US7479389B2 (en) | 2004-03-02 | 2009-01-20 | Glycofi, Inc. | ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration |
WO2005090552A2 (en) | 2004-03-17 | 2005-09-29 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
US7465577B2 (en) | 2004-04-29 | 2008-12-16 | Glycofi, Inc. | Methods for reducing or eliminating α-mannosidase resistant glycans for the production of glycoproteins |
US20060252069A1 (en) | 2005-04-21 | 2006-11-09 | Zhang Kunyan | Pcr for mrsa sccmec typing |
CA2651456A1 (en) | 2006-05-19 | 2007-11-29 | Glycofi, Inc. | Recombinant vectors |
WO2007136752A2 (en) * | 2006-05-19 | 2007-11-29 | Glycofi, Inc. | Erythropoietin compositions |
-
2010
- 2010-10-25 IN IN3823DEN2012 patent/IN2012DN03823A/en unknown
- 2010-10-25 BR BR112012009886A patent/BR112012009886A2/en not_active IP Right Cessation
- 2010-10-25 CN CN2010800519511A patent/CN102686731A/en active Pending
- 2010-10-25 US US13/503,707 patent/US20130011875A1/en not_active Abandoned
- 2010-10-25 MX MX2012004993A patent/MX2012004993A/en not_active Application Discontinuation
- 2010-10-25 RU RU2012122166/10A patent/RU2012122166A/en not_active Application Discontinuation
- 2010-10-25 EP EP20100827360 patent/EP2494053A4/en not_active Withdrawn
- 2010-10-25 WO PCT/US2010/053903 patent/WO2011053541A1/en active Application Filing
- 2010-10-25 KR KR1020127010917A patent/KR20140015137A/en not_active Application Discontinuation
- 2010-10-25 AU AU2010313608A patent/AU2010313608A1/en not_active Abandoned
- 2010-10-25 CA CA2777487A patent/CA2777487A1/en not_active Abandoned
- 2010-10-25 JP JP2012536916A patent/JP2013509180A/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
BR112012009886A2 (en) | 2015-09-15 |
CN102686731A (en) | 2012-09-19 |
EP2494053A1 (en) | 2012-09-05 |
EP2494053A4 (en) | 2013-10-30 |
RU2012122166A (en) | 2013-12-10 |
AU2010313608A1 (en) | 2012-06-07 |
KR20140015137A (en) | 2014-02-06 |
WO2011053541A1 (en) | 2011-05-05 |
CA2777487A1 (en) | 2011-05-05 |
JP2013509180A (en) | 2013-03-14 |
US20130011875A1 (en) | 2013-01-10 |
MX2012004993A (en) | 2012-06-12 |
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