CN102686731A - 生产具有改善的分泌效率的重组蛋白质的方法 - Google Patents

生产具有改善的分泌效率的重组蛋白质的方法 Download PDF

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CN102686731A
CN102686731A CN2010800519511A CN201080051951A CN102686731A CN 102686731 A CN102686731 A CN 102686731A CN 2010800519511 A CN2010800519511 A CN 2010800519511A CN 201080051951 A CN201080051951 A CN 201080051951A CN 102686731 A CN102686731 A CN 102686731A
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vps10
protein
cell
yeast
pichia pastoris
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M·米尔
H·林
B-K·蔡
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Merck Sharp and Dohme LLC
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2010800519511A 2009-10-30 2010-10-25 生产具有改善的分泌效率的重组蛋白质的方法 Pending CN102686731A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25637909P 2009-10-30 2009-10-30
US61/256379 2009-10-30
US35066810P 2010-06-02 2010-06-02
US61/350668 2010-06-02
PCT/US2010/053903 WO2011053541A1 (en) 2009-10-30 2010-10-25 Methods for the production of recombinant proteins with improved secretion efficiencies

Publications (1)

Publication Number Publication Date
CN102686731A true CN102686731A (zh) 2012-09-19

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CN2010800519511A Pending CN102686731A (zh) 2009-10-30 2010-10-25 生产具有改善的分泌效率的重组蛋白质的方法

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US (1) US20130011875A1 (enExample)
EP (1) EP2494053A4 (enExample)
JP (1) JP2013509180A (enExample)
KR (1) KR20140015137A (enExample)
CN (1) CN102686731A (enExample)
AU (1) AU2010313608A1 (enExample)
BR (1) BR112012009886A2 (enExample)
CA (1) CA2777487A1 (enExample)
IN (1) IN2012DN03823A (enExample)
MX (1) MX2012004993A (enExample)
RU (1) RU2012122166A (enExample)
WO (1) WO2011053541A1 (enExample)

Cited By (3)

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CN107810271A (zh) * 2014-11-20 2018-03-16 耶路撒冷希伯来大学伊萨姆研发公司 用于在植物细胞中生产具有改变的糖基化模式的多肽的组合物和方法
CN113862242A (zh) * 2021-10-15 2021-12-31 江南大学 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法
WO2025000334A1 (zh) * 2023-06-29 2025-01-02 中国科学院深圳先进技术研究院 融合蛋白及其在制备代谢产物中的应用

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US20120213728A1 (en) * 2009-10-30 2012-08-23 Merck Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
US9102969B2 (en) * 2011-03-11 2015-08-11 Kaneka Corporation Method for producing heterologous protein using yeast with disruption of VPS gene
US9567596B2 (en) 2012-01-05 2017-02-14 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
CN102559740B (zh) * 2012-02-25 2013-03-27 山东大学 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
WO2014136113A1 (en) 2013-03-06 2014-09-12 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
MX2016000306A (es) 2013-07-10 2016-08-08 Novartis Ag Celulas de hongos filamentosos deficientes en multiples proteasas y métodos de uso de las mismas.
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
ES2902044T3 (es) * 2015-08-05 2022-03-24 Lonza Ag Variantes de promotor
WO2018132821A2 (en) 2017-01-13 2018-07-19 Bolt Threads, Inc. Elastomeric proteins
WO2019027364A1 (en) 2017-07-31 2019-02-07 Biopetrolia Ab FUNGIC CELL WITH ENHANCED PROTEIN PRODUCTION CAPACITY
SE2051317A1 (en) * 2020-11-11 2022-05-12 Nielsen Dina Petranovic A genetically modified yeast cell
CN113604373A (zh) * 2021-02-08 2021-11-05 江南大学 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株
CN113736818A (zh) * 2021-10-08 2021-12-03 江南大学 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法
EP4194560A1 (en) 2021-12-08 2023-06-14 European Molecular Biology Laboratory Improved production of secreted proteins in fungal cells
CN115851468A (zh) * 2022-07-18 2023-03-28 江南大学 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用
CN116478905B (zh) * 2023-02-15 2025-07-22 北京中医药大学 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用

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US20080139470A1 (en) * 2006-05-19 2008-06-12 Natarajan Sethuraman Erythropoietin compositions

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107810271A (zh) * 2014-11-20 2018-03-16 耶路撒冷希伯来大学伊萨姆研发公司 用于在植物细胞中生产具有改变的糖基化模式的多肽的组合物和方法
CN107810271B (zh) * 2014-11-20 2021-09-28 耶路撒冷希伯来大学伊萨姆研发公司 用于在植物细胞中生产具有改变的糖基化模式的多肽的组合物和方法
CN113862242A (zh) * 2021-10-15 2021-12-31 江南大学 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法
WO2025000334A1 (zh) * 2023-06-29 2025-01-02 中国科学院深圳先进技术研究院 融合蛋白及其在制备代谢产物中的应用

Also Published As

Publication number Publication date
EP2494053A1 (en) 2012-09-05
US20130011875A1 (en) 2013-01-10
EP2494053A4 (en) 2013-10-30
KR20140015137A (ko) 2014-02-06
JP2013509180A (ja) 2013-03-14
RU2012122166A (ru) 2013-12-10
BR112012009886A2 (pt) 2015-09-15
AU2010313608A1 (en) 2012-06-07
CA2777487A1 (en) 2011-05-05
IN2012DN03823A (enExample) 2015-08-28
WO2011053541A1 (en) 2011-05-05
MX2012004993A (es) 2012-06-12

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