RU2011107993A - Алгоритм обнаружения для пцр-анализа - Google Patents
Алгоритм обнаружения для пцр-анализа Download PDFInfo
- Publication number
- RU2011107993A RU2011107993A RU2011107993/10A RU2011107993A RU2011107993A RU 2011107993 A RU2011107993 A RU 2011107993A RU 2011107993/10 A RU2011107993/10 A RU 2011107993/10A RU 2011107993 A RU2011107993 A RU 2011107993A RU 2011107993 A RU2011107993 A RU 2011107993A
- Authority
- RU
- Russia
- Prior art keywords
- approximately
- specified
- target nucleic
- nucleic acid
- nucleotide probe
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 36
- 239000000523 sample Substances 0.000 claims abstract 31
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 26
- 102000039446 nucleic acids Human genes 0.000 claims abstract 26
- 108020004707 nucleic acids Proteins 0.000 claims abstract 26
- 239000002773 nucleotide Substances 0.000 claims abstract 22
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 22
- 238000009396 hybridization Methods 0.000 claims abstract 5
- 238000002844 melting Methods 0.000 claims abstract 4
- 230000008018 melting Effects 0.000 claims abstract 4
- 238000004020 luminiscence type Methods 0.000 claims abstract 3
- 238000005259 measurement Methods 0.000 claims 6
- 239000007850 fluorescent dye Substances 0.000 claims 5
- 230000003993 interaction Effects 0.000 claims 4
- 239000011541 reaction mixture Substances 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- 125000004122 cyclic group Chemical group 0.000 claims 2
- 238000004925 denaturation Methods 0.000 claims 2
- 230000036425 denaturation Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 230000005855 radiation Effects 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13604008P | 2008-08-08 | 2008-08-08 | |
| US61/136,040 | 2008-08-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2011107993A true RU2011107993A (ru) | 2012-09-20 |
Family
ID=41259286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011107993/10A RU2011107993A (ru) | 2008-08-08 | 2009-08-10 | Алгоритм обнаружения для пцр-анализа |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20110151461A1 (cg-RX-API-DMAC7.html) |
| EP (1) | EP2315844A1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP2011530296A (cg-RX-API-DMAC7.html) |
| KR (1) | KR20110052681A (cg-RX-API-DMAC7.html) |
| CN (1) | CN102112632A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2009279457A1 (cg-RX-API-DMAC7.html) |
| BR (1) | BRPI0917434A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2733186A1 (cg-RX-API-DMAC7.html) |
| RU (1) | RU2011107993A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2010017543A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2009324806A1 (en) * | 2008-12-10 | 2011-07-07 | Smiths Detection Inc. | Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization |
| US20130203057A1 (en) * | 2012-01-20 | 2013-08-08 | Biohelix Corporation | Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions |
| CN107326085B (zh) | 2012-02-03 | 2022-05-03 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| EP2880184B1 (en) * | 2012-08-03 | 2021-03-31 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| WO2015147370A1 (en) * | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| KR102016747B1 (ko) * | 2014-12-09 | 2019-09-02 | 주식회사 씨젠 | 상이한 검출 온도 및 기준값을 사용한 타겟 핵산 서열의 검출 |
| CN107208139B (zh) | 2014-12-09 | 2020-11-10 | Seegene株式会社 | 与靶核酸序列有关的信号的分辨 |
| KR102110999B1 (ko) * | 2016-01-26 | 2020-05-14 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 시그널을 제공하는 방법 |
| AU2019209444A1 (en) | 2018-01-22 | 2020-07-30 | Luminex Corporation | Methods and compositions for discrete melt analysis |
| CN112424377A (zh) | 2018-04-17 | 2021-02-26 | 克罗玛科德公司 | 用于多重分析的方法和系统 |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| KR20240093623A (ko) * | 2021-10-21 | 2024-06-24 | 주식회사 씨젠 | 시료 내 표적 분석물질에 대한 양음성 판독 장치 및 방법 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5475098A (en) * | 1994-06-14 | 1995-12-12 | The United States Of America As Represented By The Department Of Health And Human Services | Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli |
| ATE440963T1 (de) * | 1998-07-02 | 2009-09-15 | Gen Probe Inc | Molekulare fackeln |
| US8900811B2 (en) * | 2000-11-16 | 2014-12-02 | Caliper Life Sciences, Inc. | Method and apparatus for generating thermal melting curves in a microfluidic device |
| US20050202470A1 (en) * | 2000-11-16 | 2005-09-15 | Caliper Life Sciences, Inc. | Binding assays using molecular melt curves |
| AU2002359329A1 (en) * | 2001-10-30 | 2003-05-12 | The Texas A And M University System | Method and apparatus for temperature gradient microfluidics |
| AU2006315489B2 (en) * | 2005-11-14 | 2012-09-06 | Gen-Probe Incorporated | Parametric calibration method |
-
2009
- 2009-08-10 CN CN2009801308017A patent/CN102112632A/zh active Pending
- 2009-08-10 JP JP2011522302A patent/JP2011530296A/ja active Pending
- 2009-08-10 AU AU2009279457A patent/AU2009279457A1/en not_active Abandoned
- 2009-08-10 KR KR1020117005036A patent/KR20110052681A/ko not_active Withdrawn
- 2009-08-10 EP EP09791325A patent/EP2315844A1/en not_active Withdrawn
- 2009-08-10 BR BRPI0917434A patent/BRPI0917434A2/pt not_active Application Discontinuation
- 2009-08-10 WO PCT/US2009/053253 patent/WO2010017543A1/en not_active Ceased
- 2009-08-10 CA CA2733186A patent/CA2733186A1/en not_active Abandoned
- 2009-08-10 RU RU2011107993/10A patent/RU2011107993A/ru not_active Application Discontinuation
- 2009-08-10 US US13/057,735 patent/US20110151461A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2009279457A1 (en) | 2010-02-11 |
| KR20110052681A (ko) | 2011-05-18 |
| CA2733186A1 (en) | 2010-02-11 |
| CN102112632A (zh) | 2011-06-29 |
| US20110151461A1 (en) | 2011-06-23 |
| EP2315844A1 (en) | 2011-05-04 |
| JP2011530296A (ja) | 2011-12-22 |
| BRPI0917434A2 (pt) | 2015-12-01 |
| WO2010017543A1 (en) | 2010-02-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA93 | Acknowledgement of application withdrawn (no request for examination) |
Effective date: 20120813 |