KR20110052681A - Ｐｃｒ 검정을 위한 검출 알고리즘 - Google Patents

Ｐｃｒ 검정을 위한 검출 알고리즘 Download PDF

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Publication number
KR20110052681A
KR20110052681A KR1020117005036A KR20117005036A KR20110052681A KR 20110052681 A KR20110052681 A KR 20110052681A KR 1020117005036 A KR1020117005036 A KR 1020117005036A KR 20117005036 A KR20117005036 A KR 20117005036A KR 20110052681 A KR20110052681 A KR 20110052681A
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KR
South Korea
Prior art keywords
nucleic acid
target nucleic
probe
label
temperature
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KR1020117005036A
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English (en)
Korean (ko)
Inventor
존 로버트 링크
배리 에드워드 보이즈
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스미스 디텍션 인크.
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Application filed by 스미스 디텍션 인크. filed Critical 스미스 디텍션 인크.
Publication of KR20110052681A publication Critical patent/KR20110052681A/ko
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
KR1020117005036A 2008-08-08 2009-08-10 Ｐｃｒ 검정을 위한 검출 알고리즘 Withdrawn KR20110052681A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13604008P 2008-08-08 2008-08-08
US61/136,040 2008-08-08

Publications (1)

Publication Number Publication Date
KR20110052681A true KR20110052681A (ko) 2011-05-18

Family

ID=41259286

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020117005036A Withdrawn KR20110052681A (ko) 2008-08-08 2009-08-10 Ｐｃｒ 검정을 위한 검출 알고리즘

Country Status (10)

Country Link
US (1) US20110151461A1 (cg-RX-API-DMAC7.html)
EP (1) EP2315844A1 (cg-RX-API-DMAC7.html)
JP (1) JP2011530296A (cg-RX-API-DMAC7.html)
KR (1) KR20110052681A (cg-RX-API-DMAC7.html)
CN (1) CN102112632A (cg-RX-API-DMAC7.html)
AU (1) AU2009279457A1 (cg-RX-API-DMAC7.html)
BR (1) BRPI0917434A2 (cg-RX-API-DMAC7.html)
CA (1) CA2733186A1 (cg-RX-API-DMAC7.html)
RU (1) RU2011107993A (cg-RX-API-DMAC7.html)
WO (1) WO2010017543A1 (cg-RX-API-DMAC7.html)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180030945A (ko) * 2014-03-28 2018-03-26 주식회사 씨젠 상이한 검출 온도를 이용한 타겟 핵산 서열의 검출
KR20180100446A (ko) * 2016-01-26 2018-09-10 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
WO2023068823A1 (ko) * 2021-10-21 2023-04-27 주식회사 씨젠 시료 내 표적 분석물질에 대한 양음성 판독 장치 및 방법

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009324806A1 (en) * 2008-12-10 2011-07-07 Smiths Detection Inc. Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization
US20130203057A1 (en) * 2012-01-20 2013-08-08 Biohelix Corporation Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
EP2880184B1 (en) * 2012-08-03 2021-03-31 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
KR102016747B1 (ko) * 2014-12-09 2019-09-02 주식회사 씨젠 상이한 검출 온도 및 기준값을 사용한 타겟 핵산 서열의 검출
CN107208139B (zh) 2014-12-09 2020-11-10 Seegene株式会社 与靶核酸序列有关的信号的分辨
AU2019209444A1 (en) 2018-01-22 2020-07-30 Luminex Corporation Methods and compositions for discrete melt analysis
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
ATE440963T1 (de) * 1998-07-02 2009-09-15 Gen Probe Inc Molekulare fackeln
US8900811B2 (en) * 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
US20050202470A1 (en) * 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
AU2002359329A1 (en) * 2001-10-30 2003-05-12 The Texas A And M University System Method and apparatus for temperature gradient microfluidics
AU2006315489B2 (en) * 2005-11-14 2012-09-06 Gen-Probe Incorporated Parametric calibration method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180030945A (ko) * 2014-03-28 2018-03-26 주식회사 씨젠 상이한 검출 온도를 이용한 타겟 핵산 서열의 검출
KR20180100446A (ko) * 2016-01-26 2018-09-10 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
WO2023068823A1 (ko) * 2021-10-21 2023-04-27 주식회사 씨젠 시료 내 표적 분석물질에 대한 양음성 판독 장치 및 방법

Also Published As

Publication number Publication date
AU2009279457A1 (en) 2010-02-11
RU2011107993A (ru) 2012-09-20
CA2733186A1 (en) 2010-02-11
CN102112632A (zh) 2011-06-29
US20110151461A1 (en) 2011-06-23
EP2315844A1 (en) 2011-05-04
JP2011530296A (ja) 2011-12-22
BRPI0917434A2 (pt) 2015-12-01
WO2010017543A1 (en) 2010-02-11

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Date Code Title Description
PA0105 International application

Patent event date: 20110303

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
PC1203 Withdrawal of no request for examination
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid