RU1800329C - Method for anabazine hydrochloride quantitative determination - Google Patents

Abstract

The inventive sample under the action of chlorodan (ammonium thiocyanate and chloramine B) in an acidic environment forms a piperidine derivative of gluta-aldehyde, which then condenses with an organic compound - dimedone in an alcohol-aqueous medium (pH 6.8-7.2) with the formation of colored products; the latter photometry at 490 nm. 1 tab.

Description

The invention relates to analytical chemistry, and in particular to methods for the quantitative determination of anabazine hydrochloride drug (s-piperidyl- / pyridine hydrochloride). Anabazine hydrochloride tablets are used as an anti-chronic nicotinism agent.

The purpose of the invention is to increase the sensitivity and accuracy of determination.

The method is carried out as follows. The analyzed sample of anabazine hydrochloride is treated with a 5% solution of ammonium thiocyanate and a 5% solution of chloramine B in an acidic medium created by 0.1 N. hydrochloric acid solution. Next, a 1% alcohol solution of dimedone (5-10 mg) in the presence of 0.1 N was added. sodium hydroxide solution; the colored reaction products photometer (I max 490 nm). The sensitivity of the reaction is 50 μg in 1 ml of solution. Beer's obedience to the law is observed in

the range of 0.1-1.0 mg of anabazine hydrochloride in 25 ml of solution.

An example of a specific implementation.

1.0 ml of the test solution of anabazine hydrochloride is added to one test tube, and 0.5 ml of the solution of a standard sample of the preparation is added to the other (0.001 g of anabazine hydrochloride is contained in 1 ml of the solution). 1 ml of 0.1 N was added to both tubes. hydrochloric acid solution, 1 ml of a 5% solution of ammonium thiocyanate, 1 ml of a 5% solution of chloramine B, 2 ml of 0.1 N. sodium hydroxide solution and 0.5 ml of a 1% alcohol solution of dimedone. Volumes were adjusted to 10 ml with alcohol. After 15-20 minutes, the optical density of the solution was measured at A 490 nm in cuvettes with a layer thickness of up to 10 mm.

The content of anabazine hydrochloride in% is calculated by the formula

fe

00

oh oh

CJ ND Yu

c Dl-Cc

100

DCT P

where Di is the relative optical density of the test solution;

CC - the content of anabazine hydrochloride in 0.5 ml of a solution of a standard sample, g;

100 - the total volume of the test solution, ml;

p is the volume of the test solution, taken for determination, ml.

Claims (1)

The claims A method for quantitative determination of anabazine hydrochloride by treating a sample with solutions of ammonium thiocyanate and chloramine B, and then with a color reagent containing a methylene group, and measuring the optical density of the colored solution, which determines the concentration of the detected substance, characterized in that, in order to increase sensitivity and the accuracy of determination, the processing of the analyzed sample with solutions of ammonium thiocyanate and chloramine B is carried out in an acidic medium created by 0.1 N hydrochloric acid solution, as a color reagent, containing methylene group, use 1% alcohol solution of dimedone in the presence of 0.1 N. sodium hydroxide solution and processing is carried out at a pH of 6.8-7.2 and the ratio of the color reagent of the analyzed sample 5: 1, and the optical density of the colored solution is measured on wavelength 490 nm. Anabazine quantification results.