PH12021552635A1 - Materials and methods for protein production - Google Patents
Materials and methods for protein productionInfo
- Publication number
- PH12021552635A1 PH12021552635A1 PH1/2021/552635A PH12021552635A PH12021552635A1 PH 12021552635 A1 PH12021552635 A1 PH 12021552635A1 PH 12021552635 A PH12021552635 A PH 12021552635A PH 12021552635 A1 PH12021552635 A1 PH 12021552635A1
- Authority
- PH
- Philippines
- Prior art keywords
- materials
- methods
- protein production
- nucleotide positions
- promoter element
- Prior art date
Links
- 239000000463 material Substances 0.000 title abstract 2
- 230000014616 translation Effects 0.000 title 1
- 108010025188 Alcohol oxidase Proteins 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03013—Alcohol oxidase (1.1.3.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Virology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Botany (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962835338P | 2019-04-17 | 2019-04-17 | |
| PCT/US2020/028858 WO2020215017A1 (en) | 2019-04-17 | 2020-04-17 | Materials and methods for protein production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PH12021552635A1 true PH12021552635A1 (en) | 2022-07-11 |
Family
ID=70614652
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PH1/2021/552635A PH12021552635A1 (en) | 2019-04-17 | 2020-04-17 | Materials and methods for protein production |
Country Status (13)
| Country | Link |
|---|---|
| US (3) | US11427932B2 (enExample) |
| EP (1) | EP3956454A1 (enExample) |
| JP (2) | JP7762567B2 (enExample) |
| KR (1) | KR20210153106A (enExample) |
| CN (1) | CN114555812A (enExample) |
| AU (2) | AU2020257270B2 (enExample) |
| BR (1) | BR112021020727A2 (enExample) |
| CA (1) | CA3137100A1 (enExample) |
| IL (1) | IL287318A (enExample) |
| MX (1) | MX2021012671A (enExample) |
| PH (1) | PH12021552635A1 (enExample) |
| SG (1) | SG11202111468SA (enExample) |
| WO (1) | WO2020215017A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107614516A (zh) | 2015-05-11 | 2018-01-19 | 非凡食品有限公司 | 用于基因工程改造甲基营养型酵母的表达构建体和方法 |
| WO2020215017A1 (en) | 2019-04-17 | 2020-10-22 | Impossible Foods Inc. | Materials and methods for protein production |
| KR20220004090A (ko) | 2019-04-25 | 2022-01-11 | 임파서블 푸즈 인크. | 헴-함유 단백질의 생산을 위한 균주 및 방법 |
| US10894812B1 (en) | 2020-09-30 | 2021-01-19 | Alpine Roads, Inc. | Recombinant milk proteins |
| IL301396A (en) | 2020-09-30 | 2023-05-01 | Nobell Foods Inc | Recombinant milk proteins and food compositions comprising the same |
| US10947552B1 (en) | 2020-09-30 | 2021-03-16 | Alpine Roads, Inc. | Recombinant fusion proteins for producing milk proteins in plants |
| EP4247959A4 (en) * | 2020-11-17 | 2024-10-09 | Ginkgo Bioworks, Inc. | METHANOL-INDUCIBLE SYNTHETIC PROMOTERS AND THEIR USE |
| WO2023069991A1 (en) | 2021-10-19 | 2023-04-27 | Eat Scifi Inc. | Plant base/animal cell hybrid meat substitute |
| MX2024007198A (es) * | 2021-12-16 | 2024-06-26 | Impossible Foods Inc | Factor genetico para aumentar la expresion de las proteinas recombinantes. |
Family Cites Families (52)
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|---|---|---|---|---|
| US4158065A (en) | 1977-12-16 | 1979-06-12 | Sugiyo Co., Ltd. | Crab meat substitute and method of preparing same |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5663143A (en) * | 1988-09-02 | 1997-09-02 | Dyax Corp. | Engineered human-derived kunitz domains that inhibit human neutrophil elastase |
| US5824511A (en) | 1995-08-01 | 1998-10-20 | University Technology Corporation | Method for enhancing the production of hemoproteins |
| PL328096A1 (en) | 1996-02-01 | 1999-01-04 | North American Vaccine | Expression of external membrane (mb3) protein of neisseria meningitidis of b group in yeasts and vaccines |
| US6100057A (en) | 1996-06-10 | 2000-08-08 | Novo Nordisk Biotech, Inc. | Method for increasing hemoprotein production in filamentous fungi |
| JP3794760B2 (ja) | 1996-07-30 | 2006-07-12 | 三井化学株式会社 | フィターゼ生産酵母 |
| US7803765B2 (en) | 1999-05-05 | 2010-09-28 | Phylogica Limited | Methods of constructing biodiverse gene fragment libraries and biological modulators isolated therefrom |
| US7498304B2 (en) * | 2000-06-16 | 2009-03-03 | Curis, Inc. | Angiogenesis-modulating compositions and uses |
| CA2525493C (en) | 2003-04-11 | 2018-10-30 | Ventria Bioscience | Human blood proteins expressed in oryza seeds |
| CN1308452C (zh) * | 2004-09-14 | 2007-04-04 | 上海永业农科生物工程有限公司 | 一种含有改组乙醇氧化酶启动子的毕赤酵母植酸酶表达系统 |
| AT501955B1 (de) * | 2005-02-23 | 2007-08-15 | Univ Graz Tech | Mutierte aox1-promotoren |
| CA2620420A1 (en) | 2005-08-24 | 2007-03-01 | The Scripps Research Institute | Translation enhancer-element dependent vector systems |
| JP2007228956A (ja) | 2006-02-28 | 2007-09-13 | Suntory Ltd | 醸造用酵母由来の有用タンパク質同定方法 |
| JP2008017733A (ja) | 2006-07-11 | 2008-01-31 | Tokyo Univ Of Agriculture | 異種タンパク質の発現方法及び異種タンパク質の発現方法により得られたタンパク質 |
| JP2008017774A (ja) | 2006-07-13 | 2008-01-31 | Nipro Corp | ピキア酵母を用いた組換えヒトヘモグロビンの製造 |
| US20100074998A1 (en) | 2006-11-01 | 2010-03-25 | Alicia Espeleta Vega | Meat Substitute Food Product And Process For Preparing The Same |
| DK2106447T3 (da) | 2007-01-26 | 2013-06-24 | Novozymes As | Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia |
| EP2058398A1 (en) | 2007-11-09 | 2009-05-13 | Nipro Corporation | Production of recombinant human hemoglobin using pichia yeast |
| CN102105589A (zh) | 2008-07-11 | 2011-06-22 | 诺维信公司 | 巴斯德毕赤酵母(Pichia pastoris) DAS启动子变体 |
| CA2765849A1 (en) | 2009-07-02 | 2011-01-06 | Verdezyne, Inc. | Biological methods for preparing adipic acid |
| EP2451960A2 (en) | 2009-07-09 | 2012-05-16 | Verdezyne, Inc. | Engineered microorganisms with enhanced fermentation activity |
| WO2011079388A1 (en) | 2009-12-30 | 2011-07-07 | Iogen Energy Corporation | Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates |
| CN102190731B (zh) | 2010-03-09 | 2016-01-27 | 中国科学院上海生命科学研究院 | 用人工转录因子诱导产生多能干细胞 |
| US9085766B2 (en) | 2010-05-21 | 2015-07-21 | Cornell University | Methods of producing recombinant heme-binding proteins and uses thereof |
| WO2012083424A1 (en) | 2010-12-21 | 2012-06-28 | The University Of Western Ontario | Novel amino acid linker sequences for ligand immobilization |
| WO2012102171A1 (ja) | 2011-01-27 | 2012-08-02 | 株式会社カネカ | 形質転換用酵母およびタンパク質の製造方法 |
| WO2012177499A1 (en) | 2011-06-23 | 2012-12-27 | The Trustees Of Princeton University | Systems for induction of gene expression and protein depletion in yeast |
| KR102724112B1 (ko) | 2011-07-12 | 2024-10-31 | 임파서블 푸즈 인크. | 소비재를 위한 방법 및 조성물 |
| US20140220217A1 (en) | 2011-07-12 | 2014-08-07 | Maraxi, Inc. | Method and compositions for consumables |
| US10039306B2 (en) | 2012-03-16 | 2018-08-07 | Impossible Foods Inc. | Methods and compositions for consumables |
| EP2565262A1 (en) | 2011-08-31 | 2013-03-06 | VTU Holding GmbH | Protein expression |
| JP6295512B2 (ja) | 2012-03-15 | 2018-03-20 | 株式会社豊田中央研究所 | 酵母における外来遺伝子の発現産物の生産方法、酵母における発現調節剤及びその利用 |
| WO2013158749A2 (en) | 2012-04-17 | 2013-10-24 | Gevo, Inc. | Engineered microorganisms with improved growth properties |
| CN102757976B (zh) | 2012-07-12 | 2014-10-08 | 华东理工大学 | 消除甲醇诱导型启动子单一甲醇碳源依赖性的方法 |
| JP5854537B2 (ja) | 2012-09-19 | 2016-02-09 | 国立大学法人大阪大学 | 肺炎球菌表面タンパク質ａを含む肺炎球菌ワクチン |
| SI2943072T1 (en) | 2013-01-11 | 2018-07-31 | Impossible Foods Inc. | Methods and compositions for affecting the flavor and aroma profile of consumables |
| WO2015020537A1 (en) | 2013-08-05 | 2015-02-12 | Agresearch Limited | A method and control system for optimising the quality of meat |
| JP6759103B2 (ja) | 2014-03-31 | 2020-09-23 | インポッシブル フーズ インコーポレイテッド | ひき肉レプリカ |
| US20160160299A1 (en) | 2014-10-31 | 2016-06-09 | Board Of Regents, The University Of Texas System | Short exogenous promoter for high level expression in fungi |
| CN107614516A (zh) | 2015-05-11 | 2018-01-19 | 非凡食品有限公司 | 用于基因工程改造甲基营养型酵母的表达构建体和方法 |
| EP3128006A1 (en) * | 2015-08-06 | 2017-02-08 | Consejo Superior de Investigaciones Cientificas (CSIC) | Tools for multiprotein complex expression in pichia pastoris |
| CA3077201A1 (en) | 2017-09-28 | 2019-04-04 | Precision Fermentation, Inc. | Methods, devices, and computer program products for yeast performance monitoring in fermentation systems |
| RU2658758C1 (ru) | 2017-10-02 | 2018-06-22 | ООО "Лаборатория Ангиофарм" | Рекомбинантная плазмида для экспрессии в дрожжах Pichia pastoris гена химерного белка ангиогенина человека и штамм дрожжей Pichia pastoris - продуцент рекомбинантного химерного белка ангиогенина человека |
| US20200325484A1 (en) | 2019-04-12 | 2020-10-15 | Delin Liang | Enhancing gene expression by linking self-amplifying transcription factor with viral 2A-like peptide |
| WO2020215017A1 (en) | 2019-04-17 | 2020-10-22 | Impossible Foods Inc. | Materials and methods for protein production |
| KR20220004090A (ko) | 2019-04-25 | 2022-01-11 | 임파서블 푸즈 인크. | 헴-함유 단백질의 생산을 위한 균주 및 방법 |
| US20210062206A1 (en) | 2019-07-08 | 2021-03-04 | The Regents Of The University Of California | Synthetic transcription factors |
| MX2024007198A (es) | 2021-12-16 | 2024-06-26 | Impossible Foods Inc | Factor genetico para aumentar la expresion de las proteinas recombinantes. |
-
2020
- 2020-04-17 WO PCT/US2020/028858 patent/WO2020215017A1/en not_active Ceased
- 2020-04-17 US US16/852,232 patent/US11427932B2/en active Active
- 2020-04-17 SG SG11202111468SA patent/SG11202111468SA/en unknown
- 2020-04-17 PH PH1/2021/552635A patent/PH12021552635A1/en unknown
- 2020-04-17 AU AU2020257270A patent/AU2020257270B2/en active Active
- 2020-04-17 BR BR112021020727A patent/BR112021020727A2/pt unknown
- 2020-04-17 EP EP20724682.8A patent/EP3956454A1/en active Pending
- 2020-04-17 MX MX2021012671A patent/MX2021012671A/es unknown
- 2020-04-17 JP JP2021561699A patent/JP7762567B2/ja active Active
- 2020-04-17 CA CA3137100A patent/CA3137100A1/en active Pending
- 2020-04-17 CN CN202080041851.4A patent/CN114555812A/zh active Pending
- 2020-04-17 KR KR1020217037340A patent/KR20210153106A/ko not_active Ceased
-
2021
- 2021-10-17 IL IL287318A patent/IL287318A/en unknown
-
2022
- 2022-07-14 US US17/812,650 patent/US12116699B2/en active Active
-
2024
- 2024-08-20 US US18/809,683 patent/US20250066951A1/en active Pending
-
2025
- 2025-03-11 JP JP2025038452A patent/JP2025102798A/ja active Pending
-
2026
- 2026-04-14 AU AU2026202808A patent/AU2026202808A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| IL287318A (en) | 2021-12-01 |
| US12116699B2 (en) | 2024-10-15 |
| US11427932B2 (en) | 2022-08-30 |
| SG11202111468SA (en) | 2021-11-29 |
| US20200332267A1 (en) | 2020-10-22 |
| US20250066951A1 (en) | 2025-02-27 |
| US20220389616A1 (en) | 2022-12-08 |
| JP7762567B2 (ja) | 2025-10-30 |
| KR20210153106A (ko) | 2021-12-16 |
| JP2022529021A (ja) | 2022-06-16 |
| MX2021012671A (es) | 2022-01-24 |
| JP2025102798A (ja) | 2025-07-08 |
| CN114555812A (zh) | 2022-05-27 |
| WO2020215017A1 (en) | 2020-10-22 |
| BR112021020727A2 (pt) | 2021-12-14 |
| AU2026202808A1 (en) | 2026-05-14 |
| EP3956454A1 (en) | 2022-02-23 |
| AU2020257270B2 (en) | 2026-01-15 |
| CA3137100A1 (en) | 2020-10-22 |
| AU2020257270A1 (en) | 2021-11-18 |
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