MX376535B - Reacción enzimática en un solo recipiente para conjugación de polipéptido doble en un solo paso usando sortasa. - Google Patents
Reacción enzimática en un solo recipiente para conjugación de polipéptido doble en un solo paso usando sortasa.Info
- Publication number
- MX376535B MX376535B MX2017007204A MX2017007204A MX376535B MX 376535 B MX376535 B MX 376535B MX 2017007204 A MX2017007204 A MX 2017007204A MX 2017007204 A MX2017007204 A MX 2017007204A MX 376535 B MX376535 B MX 376535B
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- polypeptide
- sortase
- amino acid
- sub
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 10
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 10
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 10
- 230000021615 conjugation Effects 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 238000005580 one pot reaction Methods 0.000 title 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 4
- 125000000539 amino acid group Chemical group 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 239000004471 Glycine Substances 0.000 abstract 1
- 101900206500 Staphylococcus aureus Sortase A Proteins 0.000 abstract 1
- 241000193996 Streptococcus pyogenes Species 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 108090000250 sortase A Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La presente invención se refiere a un método para producir un producto de la conjugación enzimática de tres polipéptidos, que comprende la incubación simultánea de i) un primer polipéptido que comprende la secuencia de aminoácido LPXTG (SEC ID NO: 20, en donde X puede ser cualquier residuo de aminoácido), un segundo polipéptido que comprende la secuencia de aminoácido LPXTA (SEC ID NO: 31, en donde X puede ser cualquier residuo de aminoácido), un tercer polipéptido que tiene dos extremos N-terminal por lo cual el polipéptido tiene una oligo-glicina Gm (m = 2 (SEC ID NO: 22), o 3 (SEC ID NO: 23), 0 4 (SEC ID NO: 24), o 5 (SEC ID NO: 25)) secuencias de aminoácidos en su primer extremo N-terminal y una oligo15 alanina Am (m = 2 (SEC ID NO: 26) , o 3 (SEC ID NO: 27) , o 4 (SEC ID NO: 28), o 5 (SEC ID NO: 29)) secuencias de aminoácidos en su segundo extremo N-terminal, un cuarto polipéptido con actividad de sortasa por lo cual el polipéptido se deriva de sortasa A de Staphylococcus aureus, y un quinto polipéptido con actividad de sortasa por lo cual el polipéptido se deriva de sortasa A de Streptococcus pyogenes y la recuperación del conjugado de la mezcla de reacción.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14198534 | 2014-12-17 | ||
| PCT/EP2015/079692 WO2016096787A1 (en) | 2014-12-17 | 2015-12-15 | Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2017007204A MX2017007204A (es) | 2017-08-28 |
| MX376535B true MX376535B (es) | 2025-03-07 |
Family
ID=52338849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017007204A MX376535B (es) | 2014-12-17 | 2015-12-15 | Reacción enzimática en un solo recipiente para conjugación de polipéptido doble en un solo paso usando sortasa. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US11162127B2 (es) |
| EP (1) | EP3233906B1 (es) |
| JP (1) | JP6605606B2 (es) |
| KR (1) | KR20170098821A (es) |
| CN (1) | CN107001446A (es) |
| BR (1) | BR112017010888A2 (es) |
| CA (1) | CA2966289A1 (es) |
| MX (1) | MX376535B (es) |
| RU (1) | RU2711364C2 (es) |
| SG (1) | SG11201704627XA (es) |
| WO (1) | WO2016096787A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HK1254836A1 (zh) * | 2015-10-30 | 2019-07-26 | Nbe-Therapeutics Ag | 抗-ror1抗体 |
| CN110295157A (zh) * | 2016-08-25 | 2019-10-01 | 北京大学 | 一种金黄色葡萄球菌分选酶a高效突变体 |
| WO2020002325A1 (en) * | 2018-06-25 | 2020-01-02 | Spero Therapeutics, Inc. | Compounds |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT410798B (de) * | 2001-01-26 | 2003-07-25 | Cistem Biotechnologies Gmbh | Verfahren zur identifizierung, isolierung und herstellung von antigenen gegen ein spezifisches pathogen |
| US8524865B2 (en) | 2006-05-30 | 2013-09-03 | Genentech, Inc. | Antibodies and immunoconjugates and uses therefor |
| UA95958C2 (en) * | 2006-05-30 | 2011-09-26 | Дженентек, Инк. | Antibody that binds to cd22, immunoconjugates and uses therefor |
| US8247198B2 (en) | 2007-09-21 | 2012-08-21 | Friedrich Srienc | Enzymatic processing in deep eutectic solvents |
| WO2010087994A2 (en) * | 2009-01-30 | 2010-08-05 | Whitehead Institute For Biomedical Research | Methods for ligation and uses thereof |
| ES2589769T3 (es) | 2009-02-27 | 2016-11-16 | Massachusetts Institute Of Technology | Proteínas modificadas con alta afinidad por quelatos de DOTA |
| WO2012145522A2 (en) | 2011-04-19 | 2012-10-26 | Georgia Tech Research Corporation | Deep eutectic solvent systems and methods |
| JP2014525904A (ja) * | 2011-06-28 | 2014-10-02 | ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ | タンパク質連結用クリックケミストリーハンドルを設置するためのソルターゼの使用 |
| US9588110B2 (en) | 2011-07-28 | 2017-03-07 | Cell Signaling Technology, Inc. | Multi component antibody based detection technology |
| US20150094459A1 (en) | 2012-04-12 | 2015-04-02 | Maaike Christine Kroon | Pretreatment of Lignocellulosic Biomass and Recovery of Substituents using Natural Deep Eutectic Solvents/Compound Mixtures with Low Transition Temperatures |
| CA2874436A1 (en) | 2012-05-21 | 2013-11-28 | Massachusetts Institute Of Technology | Translocation of non-natural chemical entities through anthrax protective antigen pore |
| US20140030697A1 (en) | 2012-06-14 | 2014-01-30 | Massachusetts Institute Of Technology | Sortase-mediated modification of viral surface proteins |
| US9267127B2 (en) | 2012-06-21 | 2016-02-23 | President And Fellows Of Harvard College | Evolution of bond-forming enzymes |
| EP2867254B1 (en) | 2012-06-27 | 2017-10-25 | F. Hoffmann-La Roche AG | Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof |
| KR20150023889A (ko) * | 2012-06-27 | 2015-03-05 | 에프. 호프만-라 로슈 아게 | 2 개 이상의 상이한 결합 단위를 함유하는 맞춤-제작된 고도로 선별적이고 다중-특이적인 표적화 단위의 선별 및 제조 방법 및 이의 용도 |
| WO2014055936A1 (en) | 2012-10-04 | 2014-04-10 | Integenx Inc. | Preservation of biological materials in non-aqueous fluid media |
| GB201303666D0 (en) | 2013-03-01 | 2013-04-17 | Goldsborough Andrew S | Sample fixation and stabilisation |
| EP2777714A1 (en) | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
| WO2014145441A1 (en) * | 2013-03-15 | 2014-09-18 | The Trustees Of The University Of Pennsylvania | Sortase-mediated protein purification and ligation |
| CN105722851A (zh) | 2013-04-28 | 2016-06-29 | 秦刚 | 一种新型的连接子及其制备方法和用途 |
| AU2014262469B2 (en) | 2013-05-10 | 2019-11-14 | Whitehead Institute For Biomedical Research | Protein modification of living cells using sortase |
| EP3546484B1 (en) | 2013-05-10 | 2021-09-08 | Whitehead Institute for Biomedical Research | In vitro production of red blood cells with sortaggable proteins |
-
2015
- 2015-12-15 EP EP15817154.6A patent/EP3233906B1/en active Active
- 2015-12-15 CN CN201580068342.XA patent/CN107001446A/zh active Pending
- 2015-12-15 CA CA2966289A patent/CA2966289A1/en active Pending
- 2015-12-15 SG SG11201704627XA patent/SG11201704627XA/en unknown
- 2015-12-15 BR BR112017010888A patent/BR112017010888A2/pt active Search and Examination
- 2015-12-15 JP JP2017531746A patent/JP6605606B2/ja not_active Expired - Fee Related
- 2015-12-15 RU RU2017122041A patent/RU2711364C2/ru active
- 2015-12-15 KR KR1020177015798A patent/KR20170098821A/ko not_active Ceased
- 2015-12-15 WO PCT/EP2015/079692 patent/WO2016096787A1/en not_active Ceased
- 2015-12-15 MX MX2017007204A patent/MX376535B/es active IP Right Grant
-
2017
- 2017-06-16 US US15/625,918 patent/US11162127B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20170314055A1 (en) | 2017-11-02 |
| EP3233906B1 (en) | 2019-05-01 |
| KR20170098821A (ko) | 2017-08-30 |
| SG11201704627XA (en) | 2017-07-28 |
| CN107001446A (zh) | 2017-08-01 |
| RU2711364C2 (ru) | 2020-01-16 |
| US11162127B2 (en) | 2021-11-02 |
| CA2966289A1 (en) | 2016-06-23 |
| JP6605606B2 (ja) | 2019-11-13 |
| RU2017122041A3 (es) | 2019-07-17 |
| EP3233906A1 (en) | 2017-10-25 |
| BR112017010888A2 (pt) | 2018-02-27 |
| JP2018505661A (ja) | 2018-03-01 |
| WO2016096787A1 (en) | 2016-06-23 |
| MX2017007204A (es) | 2017-08-28 |
| RU2017122041A (ru) | 2019-01-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |