SG11201704627XA - Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase - Google Patents

Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase

Info

Publication number
SG11201704627XA
SG11201704627XA SG11201704627XA SG11201704627XA SG11201704627XA SG 11201704627X A SG11201704627X A SG 11201704627XA SG 11201704627X A SG11201704627X A SG 11201704627XA SG 11201704627X A SG11201704627X A SG 11201704627XA SG 11201704627X A SG11201704627X A SG 11201704627XA
Authority
SG
Singapore
Prior art keywords
sortase
enzymatic
single step
pot reaction
polypeptide conjugation
Prior art date
Application number
SG11201704627XA
Inventor
Dieter Heindl
Erhard Kopetzki
Georg Tiefenthaler
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG11201704627XA publication Critical patent/SG11201704627XA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/2207—Sortase A (3.4.22.70)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
SG11201704627XA 2014-12-17 2015-12-15 Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase SG11201704627XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14198534 2014-12-17
PCT/EP2015/079692 WO2016096787A1 (en) 2014-12-17 2015-12-15 Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase

Publications (1)

Publication Number Publication Date
SG11201704627XA true SG11201704627XA (en) 2017-07-28

Family

ID=52338849

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201704627XA SG11201704627XA (en) 2014-12-17 2015-12-15 Enzymatic one-pot reaction for double polypeptide conjugation in a single step using sortase

Country Status (11)

Country Link
US (1) US11162127B2 (en)
EP (1) EP3233906B1 (en)
JP (1) JP6605606B2 (en)
KR (1) KR20170098821A (en)
CN (1) CN107001446A (en)
BR (1) BR112017010888A2 (en)
CA (1) CA2966289A1 (en)
MX (1) MX2017007204A (en)
RU (1) RU2711364C2 (en)
SG (1) SG11201704627XA (en)
WO (1) WO2016096787A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180069067A (en) * 2015-10-30 2018-06-22 엔비이-테라퓨틱스 아게 Anti-ROR1 antibody
CN110295157A (en) * 2016-08-25 2019-10-01 北京大学 A kind of efficient mutant of staphylococcus aureus sorting enzyme A

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT410798B (en) * 2001-01-26 2003-07-25 Cistem Biotechnologies Gmbh METHOD FOR IDENTIFYING, ISOLATING AND PRODUCING ANTIGENS AGAINST A SPECIFIC PATHOGEN
DK2032606T3 (en) 2006-05-30 2014-02-03 Genentech Inc Antibodies and immune conjugates and uses thereof
CN101490087B (en) * 2006-05-30 2013-11-06 健泰科生物技术公司 Antibodies and immunoconjugates and uses therefor
US8247198B2 (en) 2007-09-21 2012-08-21 Friedrich Srienc Enzymatic processing in deep eutectic solvents
WO2010087994A2 (en) * 2009-01-30 2010-08-05 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
ES2589769T3 (en) 2009-02-27 2016-11-16 Massachusetts Institute Of Technology Modified proteins with high affinity for DOTA chelates
WO2012145522A2 (en) 2011-04-19 2012-10-26 Georgia Tech Research Corporation Deep eutectic solvent systems and methods
JP2014525904A (en) * 2011-06-28 2014-10-02 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ Using sortase to install click chemistry handles for protein ligation
US9588110B2 (en) 2011-07-28 2017-03-07 Cell Signaling Technology, Inc. Multi component antibody based detection technology
WO2013153203A1 (en) 2012-04-12 2013-10-17 Technische Universiteit Eindhoven Pretreatment of lignocellulosic biomass and recovery of substituents using natural deep eutectic solvents/compound mixtures with low transition temperatures
US9731029B2 (en) 2012-05-21 2017-08-15 Massachusetts Institute Of Technology Protein retrosplicing enabled by a double ligation reaction
US20140030697A1 (en) 2012-06-14 2014-01-30 Massachusetts Institute Of Technology Sortase-mediated modification of viral surface proteins
US9267127B2 (en) 2012-06-21 2016-02-23 President And Fellows Of Harvard College Evolution of bond-forming enzymes
BR112014028368A2 (en) 2012-06-27 2017-11-14 Hoffmann La Roche method of producing antibody fc region conjugate, antibody fc region conjugate and pharmaceutical formulation
EP2867253B1 (en) * 2012-06-27 2016-09-14 F. Hoffmann-La Roche AG Method for selection and production of tailor-made highly selective and multi-specific targeting entities containing at least two different binding entities and uses thereof
WO2014055936A1 (en) 2012-10-04 2014-04-10 Integenx Inc. Preservation of biological materials in non-aqueous fluid media
GB201303666D0 (en) 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
EP2777714A1 (en) 2013-03-15 2014-09-17 NBE-Therapeutics LLC Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme
WO2014145441A1 (en) * 2013-03-15 2014-09-18 The Trustees Of The University Of Pennsylvania Sortase-mediated protein purification and ligation
US20180104349A9 (en) 2013-04-28 2018-04-19 Gang Qin Novel linker, preparation method, and application thereof
EP4119662A1 (en) 2013-05-10 2023-01-18 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
EP3693398A1 (en) 2013-05-10 2020-08-12 Whitehead Institute for Biomedical Research In vitro production of red blood cells with sortaggable proteins

Also Published As

Publication number Publication date
EP3233906B1 (en) 2019-05-01
CA2966289A1 (en) 2016-06-23
BR112017010888A2 (en) 2018-02-27
JP2018505661A (en) 2018-03-01
EP3233906A1 (en) 2017-10-25
US11162127B2 (en) 2021-11-02
JP6605606B2 (en) 2019-11-13
RU2711364C2 (en) 2020-01-16
RU2017122041A3 (en) 2019-07-17
CN107001446A (en) 2017-08-01
US20170314055A1 (en) 2017-11-02
MX2017007204A (en) 2017-08-28
WO2016096787A1 (en) 2016-06-23
KR20170098821A (en) 2017-08-30
RU2017122041A (en) 2019-01-21

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