SG11201704625UA - Novel methods for enzyme mediated polypeptide conjugation using sortase - Google Patents

Novel methods for enzyme mediated polypeptide conjugation using sortase

Info

Publication number
SG11201704625UA
SG11201704625UA SG11201704625UA SG11201704625UA SG11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA
Authority
SG
Singapore
Prior art keywords
sortase
novel methods
enzyme mediated
polypeptide conjugation
mediated polypeptide
Prior art date
Application number
SG11201704625UA
Inventor
Mara Boenitz-Dulat
Peter Kratzsch
Martin Schatte
Original Assignee
F Hoffmann-La Roche Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP14198532 priority Critical
Application filed by F Hoffmann-La Roche Ag filed Critical F Hoffmann-La Roche Ag
Priority to PCT/EP2015/079617 priority patent/WO2016096741A1/en
Publication of SG11201704625UA publication Critical patent/SG11201704625UA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22Cysteine endopeptidases (3.4.22)
    • C12Y304/2207Sortase A (3.4.22.70)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472Cysteine endopeptidases (3.4.22)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
SG11201704625UA 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase SG11201704625UA (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP14198532 2014-12-17
PCT/EP2015/079617 WO2016096741A1 (en) 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase

Publications (1)

Publication Number Publication Date
SG11201704625UA true SG11201704625UA (en) 2017-07-28

Family

ID=52338848

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201704625UA SG11201704625UA (en) 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase

Country Status (11)

Country Link
US (1) US10190182B2 (en)
EP (1) EP3233905A1 (en)
JP (1) JP2018500901A (en)
KR (1) KR20170094216A (en)
CN (1) CN107001447A (en)
BR (1) BR112017010775A2 (en)
CA (1) CA2966287A1 (en)
MX (1) MX2017007503A (en)
RU (1) RU2017122029A3 (en)
SG (1) SG11201704625UA (en)
WO (1) WO2016096741A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106191015B (en) * 2016-08-25 2019-09-13 北京大学 The efficient mutant of staphylococcus aureus sorting enzyme A

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US4361544A (en) 1980-03-03 1982-11-30 Goldenberg Milton David Tumor localization and therapy with labeled antibodies specific to intracellular tumor-associated markers
US5342606A (en) 1984-10-18 1994-08-30 Board Of Regents, The University Of Texas System Polyazamacrocyclic compounds for complexation of metal ions
US5316757A (en) 1984-10-18 1994-05-31 Board Of Regents, The University Of Texas System Synthesis of polyazamacrocycles with more than one type of side-chain chelating groups
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US7018809B1 (en) 1991-09-19 2006-03-28 Genentech, Inc. Expression of functional antibody fragments
US5428139A (en) 1991-12-10 1995-06-27 The Dow Chemical Company Bicyclopolyazamacrocyclophosphonic acid complexes for use as radiopharmaceuticals
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RU2139731C1 (en) 1992-11-13 1999-10-20 Айдек Фармасьютикалс Корпорейшн (US Methods of treatment, antibodies, hybridoma
US5462725A (en) 1993-05-06 1995-10-31 The Dow Chemical Company 2-pyridylmethylenepolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents
US5385893A (en) 1993-05-06 1995-01-31 The Dow Chemical Company Tricyclopolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
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US5834456A (en) 1996-02-23 1998-11-10 The Dow Chemical Company Polyazamacrocyclofluoromonoalkylphosphonic acids, and their complexes, for use as contrast agents
US6528624B1 (en) 1998-04-02 2003-03-04 Genentech, Inc. Polypeptide variants
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
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US20100111856A1 (en) 2004-09-23 2010-05-06 Herman Gill Zirconium-radiolabeled, cysteine engineered antibody conjugates
EP2391714B2 (en) 2009-01-30 2019-07-24 Whitehead Institute for Biomedical Research Methods for ligation and uses thereof
EP2403530B1 (en) 2009-02-27 2016-05-11 Massachusetts Institute of Technology Engineered proteins with high affinity for dota chelates
CN103764665A (en) 2011-06-28 2014-04-30 怀特黑德生物医学研究所 Using sortases to install click chemistry handles for protein ligation
BR112014032193A2 (en) 2012-06-27 2017-06-27 Hoffmann La Roche bispecific antibody production and combination determination methods, bispecific antibody, formulation and use of bispecific antibody

Also Published As

Publication number Publication date
RU2017122029A3 (en) 2019-07-17
WO2016096741A1 (en) 2016-06-23
EP3233905A1 (en) 2017-10-25
US10190182B2 (en) 2019-01-29
BR112017010775A2 (en) 2018-02-27
CA2966287A1 (en) 2016-06-23
CN107001447A (en) 2017-08-01
JP2018500901A (en) 2018-01-18
US20170292166A1 (en) 2017-10-12
KR20170094216A (en) 2017-08-17
RU2017122029A (en) 2019-01-17
MX2017007503A (en) 2017-10-04

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