SG11201704625UA - Novel methods for enzyme mediated polypeptide conjugation using sortase - Google Patents
Novel methods for enzyme mediated polypeptide conjugation using sortaseInfo
- Publication number
- SG11201704625UA SG11201704625UA SG11201704625UA SG11201704625UA SG11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA SG 11201704625U A SG11201704625U A SG 11201704625UA
- Authority
- SG
- Singapore
- Prior art keywords
- sortase
- novel methods
- enzyme mediated
- polypeptide conjugation
- mediated polypeptide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6472—Cysteine endopeptidases (3.4.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP14198532 | 2014-12-17 | ||
| PCT/EP2015/079617 WO2016096741A1 (en) | 2014-12-17 | 2015-12-14 | Novel methods for enzyme mediated polypeptide conjugation using sortase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201704625UA true SG11201704625UA (en) | 2017-07-28 |
Family
ID=52338848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201704625UA SG11201704625UA (en) | 2014-12-17 | 2015-12-14 | Novel methods for enzyme mediated polypeptide conjugation using sortase |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10190182B2 (en) |
| EP (1) | EP3233905B1 (en) |
| JP (1) | JP6618539B2 (en) |
| KR (1) | KR20170094216A (en) |
| CN (1) | CN107001447B (en) |
| BR (1) | BR112017010775A2 (en) |
| CA (1) | CA2966287A1 (en) |
| MX (1) | MX2017007503A (en) |
| RU (1) | RU2714963C2 (en) |
| SG (1) | SG11201704625UA (en) |
| WO (1) | WO2016096741A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110295157A (en) * | 2016-08-25 | 2019-10-01 | 北京大学 | A kind of efficient mutant of staphylococcus aureus sorting enzyme A |
| CN116888258A (en) * | 2021-02-04 | 2023-10-13 | 西湖生物医药科技(杭州)有限公司 | Modified erythrocytes and their use for treating hyperuricemia and gout |
| WO2023134573A1 (en) * | 2022-01-12 | 2023-07-20 | Westlake Therapeutics (Shanghai) Co., Limited | Modified cells and uses thereof |
| CN114487409B (en) * | 2022-04-14 | 2022-07-19 | 启德医药科技（苏州）有限公司 | Detection method and detection kit for activity of transpeptidase |
| CN116949004B (en) * | 2023-07-13 | 2024-04-09 | 苏州源启生物科技有限公司 | Transpeptidase Sortase A and preparation method and application thereof |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4150149A (en) | 1976-11-29 | 1979-04-17 | Professional Staff Association Of The Los Angeles County Harbor General Hospital | Method and means for the early detection and diagnosis of certain types of cancers |
| US4275149A (en) | 1978-11-24 | 1981-06-23 | Syva Company | Macromolecular environment control in specific receptor assays |
| US4318980A (en) | 1978-04-10 | 1982-03-09 | Miles Laboratories, Inc. | Heterogenous specific binding assay employing a cycling reactant as label |
| US4361544A (en) | 1980-03-03 | 1982-11-30 | Goldenberg Milton David | Tumor localization and therapy with labeled antibodies specific to intracellular tumor-associated markers |
| US4444744A (en) | 1980-03-03 | 1984-04-24 | Goldenberg Milton David | Tumor localization and therapy with labeled antibodies to cell surface antigens |
| US5342606A (en) | 1984-10-18 | 1994-08-30 | Board Of Regents, The University Of Texas System | Polyazamacrocyclic compounds for complexation of metal ions |
| US5316757A (en) | 1984-10-18 | 1994-05-31 | Board Of Regents, The University Of Texas System | Synthesis of polyazamacrocycles with more than one type of side-chain chelating groups |
| US4737456A (en) | 1985-05-09 | 1988-04-12 | Syntex (U.S.A.) Inc. | Reducing interference in ligand-receptor binding assays |
| US5959177A (en) | 1989-10-27 | 1999-09-28 | The Scripps Research Institute | Transgenic plants expressing assembled secretory antibodies |
| WO1993006217A1 (en) | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
| US5428139A (en) | 1991-12-10 | 1995-06-27 | The Dow Chemical Company | Bicyclopolyazamacrocyclophosphonic acid complexes for use as radiopharmaceuticals |
| US5480990A (en) | 1991-12-10 | 1996-01-02 | The Dow Chemical Company | Bicyclopolyazamacrocyclocarboxylic acid complexes for use as contrast agents |
| US5739294A (en) | 1991-12-10 | 1998-04-14 | The Dow Chemical Company | Bicyclopol yazamacrocyclophosphonic acid complexes for use as contrast agents |
| ZA932522B (en) | 1992-04-10 | 1993-12-20 | Res Dev Foundation | Immunotoxins directed against c-erbB-2(HER/neu) related surface antigens |
| ATE196606T1 (en) | 1992-11-13 | 2000-10-15 | Idec Pharma Corp | THERAPEUTIC USE OF CHIMERIC AND LABELED ANTIBODIES DIRECTED AGAINST A DIFFERENTIATION ANTIGEN WHICH EXPRESSION IS RESTRICTED TO HUMAN B LYMPHOCYTES, FOR THE TREATMENT OF B-CELL LYMPHOMA |
| US5462725A (en) | 1993-05-06 | 1995-10-31 | The Dow Chemical Company | 2-pyridylmethylenepolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents |
| US5385893A (en) | 1993-05-06 | 1995-01-31 | The Dow Chemical Company | Tricyclopolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents |
| US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5840523A (en) | 1995-03-01 | 1998-11-24 | Genetech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
| US5834456A (en) | 1996-02-23 | 1998-11-10 | The Dow Chemical Company | Polyazamacrocyclofluoromonoalkylphosphonic acids, and their complexes, for use as contrast agents |
| US6040498A (en) | 1998-08-11 | 2000-03-21 | North Caroline State University | Genetically engineered duckweed |
| US6528624B1 (en) | 1998-04-02 | 2003-03-04 | Genentech, Inc. | Polypeptide variants |
| ATE303445T1 (en) | 1999-10-04 | 2005-09-15 | Medicago Inc | METHOD FOR REGULATION OF TRANSCRIPTION OF FOREIGN GENES IN THE PRESENCE OF NITROGEN |
| US7125978B1 (en) | 1999-10-04 | 2006-10-24 | Medicago Inc. | Promoter for regulating expression of foreign genes |
| US20100111856A1 (en) | 2004-09-23 | 2010-05-06 | Herman Gill | Zirconium-radiolabeled, cysteine engineered antibody conjugates |
| ZA200809776B (en) * | 2006-05-30 | 2010-03-31 | Genentech Inc | Antibodies and immunoconjugates and uses therefor |
| WO2010087994A2 (en) | 2009-01-30 | 2010-08-05 | Whitehead Institute For Biomedical Research | Methods for ligation and uses thereof |
| US8648176B2 (en) | 2009-02-27 | 2014-02-11 | Massachusetts Institute Of Technology | Engineered proteins with high affinity for DOTA chelates |
| WO2013003555A1 (en) | 2011-06-28 | 2013-01-03 | Whitehead Institute For Biomedical Research | Using sortases to install click chemistry handles for protein ligation |
| BR112014032193A2 (en) * | 2012-06-27 | 2017-06-27 | Hoffmann La Roche | bispecific antibody production and combination determination methods, bispecific antibody, formulation and use of bispecific antibody |
-
2015
- 2015-12-14 CA CA2966287A patent/CA2966287A1/en active Pending
- 2015-12-14 BR BR112017010775A patent/BR112017010775A2/en active Search and Examination
- 2015-12-14 JP JP2017531742A patent/JP6618539B2/en active Active
- 2015-12-14 RU RU2017122029A patent/RU2714963C2/en active
- 2015-12-14 EP EP15817140.5A patent/EP3233905B1/en active Active
- 2015-12-14 MX MX2017007503A patent/MX2017007503A/en unknown
- 2015-12-14 WO PCT/EP2015/079617 patent/WO2016096741A1/en active Application Filing
- 2015-12-14 KR KR1020177016141A patent/KR20170094216A/en active Search and Examination
- 2015-12-14 SG SG11201704625UA patent/SG11201704625UA/en unknown
- 2015-12-14 CN CN201580068354.2A patent/CN107001447B/en active Active
-
2017
- 2017-06-16 US US15/625,975 patent/US10190182B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3233905A1 (en) | 2017-10-25 |
| CN107001447B (en) | 2021-07-23 |
| JP6618539B2 (en) | 2019-12-11 |
| MX2017007503A (en) | 2017-10-04 |
| CN107001447A (en) | 2017-08-01 |
| US10190182B2 (en) | 2019-01-29 |
| EP3233905B1 (en) | 2020-01-29 |
| RU2017122029A (en) | 2019-01-17 |
| BR112017010775A2 (en) | 2018-02-27 |
| RU2714963C2 (en) | 2020-02-21 |
| WO2016096741A1 (en) | 2016-06-23 |
| KR20170094216A (en) | 2017-08-17 |
| RU2017122029A3 (en) | 2019-07-17 |
| US20170292166A1 (en) | 2017-10-12 |
| JP2018500901A (en) | 2018-01-18 |
| CA2966287A1 (en) | 2016-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL251194A0 (en) | Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria | |
| IL247096A0 (en) | Multimeric fc proteins | |
| EP3122371A4 (en) | Fgf23 fusion proteins | |
| HK1232136A1 (en) | P97-ids fusion proteins p97-ids | |
| IL247246A0 (en) | Multimeric fc proteins | |
| HK1232234A1 (en) | P97 fusion proteins p97 | |
| EP3149176A4 (en) | Methods for altering polypeptide expression | |
| HRP20182029T1 (en) | Uti fusion proteins | |
| EP3164410B8 (en) | Protein recovery | |
| SG11201704625UA (en) | Novel methods for enzyme mediated polypeptide conjugation using sortase | |
| EP3204427A4 (en) | Chimeric proteins | |
| SG11201606501PA (en) | Nanobody-fluorescent protein fusion | |
| IL246533A0 (en) | Novel polypeptides | |
| AU2014904028A0 (en) | Chimeric proteins | |
| EP3221449A4 (en) | Process for refolding recombinant chymotrypsin | |
| GB201418989D0 (en) | Novel conjugation process and reagents | |
| GB201418186D0 (en) | Novel conjugation process and reagents | |
| GB201419237D0 (en) | Novel polypeptides | |
| GB201412797D0 (en) | Proteins | |
| GB201412649D0 (en) | Proteins | |
| GB201412648D0 (en) | Proteins | |
| GB201412646D0 (en) | Proteins | |
| GB201406894D0 (en) | Proteins | |
| GB201405955D0 (en) | Proteins | |
| GB201405952D0 (en) | Proteins |