PE20180743A1 - Metodo para produccion de l-aminoacido - Google Patents

Metodo para produccion de l-aminoacido

Info

Publication number
PE20180743A1
PE20180743A1 PE2017001642A PE2017001642A PE20180743A1 PE 20180743 A1 PE20180743 A1 PE 20180743A1 PE 2017001642 A PE2017001642 A PE 2017001642A PE 2017001642 A PE2017001642 A PE 2017001642A PE 20180743 A1 PE20180743 A1 PE 20180743A1
Authority
PE
Peru
Prior art keywords
amino acid
acid
gene
transporter
bacterium
Prior art date
Application number
PE2017001642A
Other languages
English (en)
Inventor
Mika Moriya
Kazuyuki Hayashi
Akari Yokokawa
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of PE20180743A1 publication Critical patent/PE20180743A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C227/28—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton from natural products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/34—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton containing six-membered aromatic rings
    • C07C229/36—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton containing six-membered aromatic rings with at least one amino group and one carboxyl group bound to the same carbon atom of the carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Referido a un metodo para producir un L-aminoacido, el cual comprende, cultivar una bacteria que tiene una capacidad productora de L-aminoacidos; y recolectar el L-aminoacido de medio y/o las celulas, en donde la bacteria ha sido modificada de manera que la actividad del transportador de la captacion de acido dicarboxilico C4 aumenta en comparacion con una cepa no modificada, en donde el transportador de captacion de acido dicarboxilico C4 consiste en uno o mas tipos de proteinas seleccionadas entre proteinas codificadas por un gen dctA, un gen dcuA y un gen dcuB, y en donde el L-aminoacido es un L-aminoacido distinto del acido L-aspartico, siempre que el L-aminoacido sea acido L-glutamico cuando el transportador de la incorporacion de acido Dicarboxilico C4 es una proteina codificada por un gen dctA
PE2017001642A 2016-10-03 2017-10-02 Metodo para produccion de l-aminoacido PE20180743A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2016195818 2016-10-03

Publications (1)

Publication Number Publication Date
PE20180743A1 true PE20180743A1 (es) 2018-04-27

Family

ID=61701255

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2017001642A PE20180743A1 (es) 2016-10-03 2017-10-02 Metodo para produccion de l-aminoacido

Country Status (6)

Country Link
US (1) US10428359B2 (es)
JP (1) JP2018057376A (es)
CN (1) CN107893089B (es)
BR (1) BR102017020967A2 (es)
FR (1) FR3056989B1 (es)
PE (1) PE20180743A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109007846A (zh) * 2018-06-20 2018-12-18 广东肇庆星湖生物科技股份有限公司 一种限制性氨基酸营养复合包及其制备方法
CN109020854B (zh) * 2018-10-17 2020-10-27 浙江工业大学 一种从发酵液中提取l-蛋氨酸的方法
KR102075160B1 (ko) * 2018-12-13 2020-02-10 대상 주식회사 L-글루탐산 생산능이 향상된 변이 균주 및 이를 이용한 l-글루탐산의 제조 방법
CN110218710B (zh) * 2019-04-30 2020-06-23 中国科学院天津工业生物技术研究所 活性提高的磷酸转酮酶及在生产代谢物中的应用
CN111363694B (zh) * 2020-01-21 2022-05-06 中国科学院城市环境研究所 一株停滞棒杆菌、筛选方法及其用途
CN111471638B (zh) * 2020-05-22 2021-11-23 江南大学 一株产l-高丝氨酸的谷氨酸棒杆菌突变株的构建与应用
CN112695036B (zh) * 2021-03-23 2021-07-06 中国科学院天津工业生物技术研究所 一种天冬氨酸激酶基因表达调控序列及其应用
EP4098658B1 (en) * 2021-04-12 2024-09-04 CJ CheilJedang Corporation Novel protein variant and method for producing l-lysine using same
CN113046398A (zh) * 2021-05-18 2021-06-29 通辽梅花生物科技有限公司 一种稳定高效生产l-异亮氨酸的发酵方法及发酵稳定剂
CN114107141B (zh) * 2021-08-19 2022-07-12 中国科学院天津工业生物技术研究所 高产l-脯氨酸的谷氨酸棒杆菌以及高产l-脯氨酸的方法
CN116814514B (zh) * 2021-08-27 2024-07-16 胡丹 提高大肠杆菌l-赖氨酸发酵产量的方法
KR20230042953A (ko) * 2021-09-23 2023-03-30 씨제이제일제당 (주) 고농도 l-글루탐산을 생산하기 위한 균주 및 이를 이용한 l-글루탐산 생산방법
CN116536226A (zh) * 2022-01-26 2023-08-04 廊坊梅花生物技术开发有限公司 产苏氨酸工程菌的构建方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030124685A1 (en) * 1999-02-01 2003-07-03 Yoko Kuwabara Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing l-arginine
AU2001293731A1 (en) 2000-09-19 2002-04-02 Degussa A.G. Nucleotide sequences coding for the dcta gene
DE10162650A1 (de) 2001-12-20 2003-07-03 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
DE10163167A1 (de) * 2001-12-21 2003-07-03 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren und Verwendung coryneformer Bakterien
RU2268300C2 (ru) * 2003-04-07 2006-01-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ, ОБЛАДАЮЩИХ ПОВЫШЕННОЙ ЭКСПРЕССИЕЙ ГЕНА pckA
ES2341264T3 (es) 2004-08-10 2010-06-17 Ajinomoto Co., Inc. El uso de fosfocetolasa para producir metabolitos utiles.
KR100948246B1 (ko) * 2004-12-28 2010-03-18 아지노모토 가부시키가이샤 Ｌ-글루탐산 생산 미생물 및 ｌ-글루탐산의 제조방법
US7794989B2 (en) * 2004-12-28 2010-09-14 Ajinomoto Co., Inc. L-glutamic acid-producing microorganism and a method for producing L-glutamic acid
CN101278041A (zh) * 2005-08-05 2008-10-01 密执安州大学 来自actinobacillus succinogenes130z（atcc 55618）用于从a.succinogenes c4－途径生产化学产品的基因
WO2007086608A1 (en) * 2006-01-27 2007-08-02 Ajinomoto Co., Inc. Method for producing l-amino acid
JP2010041920A (ja) * 2006-12-19 2010-02-25 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
RU2471870C2 (ru) 2010-06-03 2013-01-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА И L-ЦИТРУЛЛИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА pepA
WO2014185430A1 (ja) 2013-05-13 2014-11-20 味の素株式会社 Ｌ－アミノ酸の製造法
WO2015060314A1 (ja) 2013-10-21 2015-04-30 味の素株式会社 Ｌ－アミノ酸の製造法
JP6623690B2 (ja) 2015-10-30 2019-12-25 味の素株式会社 グルタミン酸系ｌ−アミノ酸の製造法

Also Published As

Publication number Publication date
JP2018057376A (ja) 2018-04-12
CN107893089A (zh) 2018-04-10
US20180094285A1 (en) 2018-04-05
BR102017020967A2 (pt) 2019-01-22
FR3056989A1 (fr) 2018-04-06
US10428359B2 (en) 2019-10-01
FR3056989B1 (fr) 2021-03-12
CN107893089B (zh) 2021-11-02

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