PE20020259A1 - Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas - Google Patents
Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellasInfo
- Publication number
- PE20020259A1 PE20020259A1 PE2001000858A PE2001000858A PE20020259A1 PE 20020259 A1 PE20020259 A1 PE 20020259A1 PE 2001000858 A PE2001000858 A PE 2001000858A PE 2001000858 A PE2001000858 A PE 2001000858A PE 20020259 A1 PE20020259 A1 PE 20020259A1
- Authority
- PE
- Peru
- Prior art keywords
- nucleic acid
- plant
- nicotine
- tobacco plant
- refers
- Prior art date
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8227—Root-specific
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1077—Pentosyltransferases (2.4.2)
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Pharmacology & Pharmacy (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
SE REFIERE A UNA MOLECULA DE ACIDO NUCLEICO AISLADO COMPUESTA POR EL ELEMENTO QUE ES PRODUCTO DE UN GEN NIC RECEPTOR SELECCIONADO DE: a) ACIDOS NUCLEICOS AISLADOS TENIENDO UNA SECUENCIA SEQ ID NO:1; b) ACIDOS NUCLEICOS AISLADOS QUE HIBRIDIZAN EL COMPLEMENTO DE SEQ ID NO:1 Y SON RECEPTORES DEL ELEMENTO QUE ES PRODUCTO DE UN GEN NIC. EL ACIDO NUCLEICO ES ADN LINEAL, CIRCULAR. SE REFIERE TAMBIEN A UN VECTOR AGROBACTERIUM O LA CELULA DE UNA PLANTA QUE CONTIENEN EL ACIDO NUCLEICO. SE REFIERE ADEMAS A UN METODO PARA OBTENER UNA PLANTA DE TABACO TRANSGENICA CON REDUCCION DEL CONTENIDO DE NICOTINA QUE COMPRENDE: a) INTRODUCIR UN ACIDO NUCLEICO EXOGENO CONTENIENDO EL ELEMENTO QUE ES PRODUCTO DE UN GEN NIC RECEPTOR EN AL MENOS DOS CELULAS DE LA PLANTA DE TABACO PARA PRODUCIR POR LO MENOS UNA CELULA TRANSFORMADA DE LA PLANTA DEL TABACO; b) TOMAR AL MENOS UNA CELULA TRANSFORMADA DE LA PLANTA DE TABACO CONTENIENDO UN ACIDO NUCLEICO EXOGENO EN UN NUMERO SUFICIENTE DE COPIAS PARA REDUCIR EL NIVEL DE NICOTINA DE LA PLANTA DE TABACO REGENERADA Y c) COMPARAR EL NIVEL DE NICOTINA QUE PODRIA PRESENTAR EN LA AUSENCIA DE UN ACIDO NUCLEICO EXOGENO. FINALMENTE CREAR UNA PLANTA ALTERANDO EL CONTENIDO DE UNA PROTEINA QUE ES REGULADA POR UN ELEMENTO DE ACTIVACION CIS SELECCIONADO DE UAS-1 QUE UNE UN COMPUESTO ACTIVADOR EL CUAL AUMENTA LA EXPRESION DE DICHA PROTEINA Y QUE CON POR LO MENOS UNA CELULA TRANSFORMADA DE LA PLANTA CONTIENE EL ACIDO NUCLEICO EXOGENO
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22919800P | 2000-08-30 | 2000-08-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
PE20020259A1 true PE20020259A1 (es) | 2002-03-30 |
Family
ID=22860199
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PE2001000858A PE20020259A1 (es) | 2000-08-30 | 2001-09-12 | Plantas transgenicas que contienen factores enzimaticos moleculares que alteran el contenido proteico en ellas |
Country Status (18)
Country | Link |
---|---|
US (8) | US7192771B2 (es) |
EP (2) | EP1724355A3 (es) |
JP (1) | JP2004507250A (es) |
KR (1) | KR20030029885A (es) |
CN (1) | CN1330753C (es) |
AR (1) | AR030513A1 (es) |
AT (1) | ATE333506T1 (es) |
AU (1) | AU2001286843A1 (es) |
CA (1) | CA2420724A1 (es) |
DE (1) | DE60121603T2 (es) |
EA (1) | EA200300317A1 (es) |
ES (1) | ES2267809T3 (es) |
GT (1) | GT200100176A (es) |
HN (1) | HN2001000250A (es) |
IL (1) | IL154678A0 (es) |
PE (1) | PE20020259A1 (es) |
UY (1) | UY26917A1 (es) |
WO (1) | WO2002018607A2 (es) |
Families Citing this family (159)
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JP2004507250A (ja) * | 2000-08-30 | 2004-03-11 | ノース・キャロライナ・ステイト・ユニヴァーシティ | タンパク質含量を変化させる分子デコイを含有するトランスジェニック植物 |
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CA2435972C (en) * | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
KR20040019301A (ko) * | 2001-06-08 | 2004-03-05 | 벡터 토바코 리미티드 | 담배에서 니코틴 및 니트로사민 수준의 조절 |
US20060157072A1 (en) * | 2001-06-08 | 2006-07-20 | Anthony Albino | Method of reducing the harmful effects of orally or transdermally delivered nicotine |
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PL1959011T3 (pl) | 2003-10-24 | 2012-07-31 | Selexis Sa | Wysokowydajny transfer genu i ekspresja w komórkach ssaczych przez procedurę wielokrotnej transfekcji sekwencji regionu przylegania do macierzy |
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JP2006315017A (ja) * | 2005-05-11 | 2006-11-24 | Canon Inc | レーザ切断方法および被切断部材 |
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-
2001
- 2001-08-28 JP JP2002522513A patent/JP2004507250A/ja active Pending
- 2001-08-28 ES ES01966318T patent/ES2267809T3/es not_active Expired - Lifetime
- 2001-08-28 EA EA200300317A patent/EA200300317A1/ru unknown
- 2001-08-28 AU AU2001286843A patent/AU2001286843A1/en not_active Abandoned
- 2001-08-28 WO PCT/US2001/026788 patent/WO2002018607A2/en active IP Right Grant
- 2001-08-28 AT AT01966318T patent/ATE333506T1/de not_active IP Right Cessation
- 2001-08-28 US US10/363,069 patent/US7192771B2/en not_active Expired - Lifetime
- 2001-08-28 EP EP06116949A patent/EP1724355A3/en not_active Withdrawn
- 2001-08-28 CN CNB018181465A patent/CN1330753C/zh not_active Expired - Fee Related
- 2001-08-28 CA CA002420724A patent/CA2420724A1/en not_active Abandoned
- 2001-08-28 EP EP01966318A patent/EP1313868B1/en not_active Expired - Lifetime
- 2001-08-28 KR KR10-2003-7002995A patent/KR20030029885A/ko not_active Application Discontinuation
- 2001-08-28 DE DE60121603T patent/DE60121603T2/de not_active Expired - Lifetime
- 2001-08-28 US US09/941,042 patent/US6911541B2/en not_active Expired - Lifetime
- 2001-08-29 GT GT200100176A patent/GT200100176A/es unknown
- 2001-08-29 AR ARP010104110A patent/AR030513A1/es unknown
- 2001-08-30 UY UY26917A patent/UY26917A1/es not_active Application Discontinuation
- 2001-08-30 IL IL15467801A patent/IL154678A0/xx unknown
- 2001-09-12 PE PE2001000858A patent/PE20020259A1/es not_active Application Discontinuation
- 2001-10-31 HN HN2001000250A patent/HN2001000250A/es unknown
-
2004
- 2004-11-10 US US10/985,401 patent/US20060057723A1/en not_active Abandoned
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2006
- 2006-05-03 US US11/417,079 patent/US20060242730A1/en not_active Abandoned
- 2006-05-03 US US11/416,569 patent/US20060191039A1/en not_active Abandoned
- 2006-05-03 US US11/416,576 patent/US20060195936A1/en not_active Abandoned
- 2006-06-21 US US11/471,754 patent/US20060236434A1/en not_active Abandoned
- 2006-09-20 US US11/524,143 patent/US20070016975A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1724355A3 (en) | 2007-05-23 |
KR20030029885A (ko) | 2003-04-16 |
ATE333506T1 (de) | 2006-08-15 |
US20060242730A1 (en) | 2006-10-26 |
UY26917A1 (es) | 2001-10-25 |
WO2002018607A2 (en) | 2002-03-07 |
EP1313868A2 (en) | 2003-05-28 |
DE60121603T2 (de) | 2007-06-21 |
WO2002018607A3 (en) | 2003-02-27 |
AR030513A1 (es) | 2003-08-20 |
US20030018997A1 (en) | 2003-01-23 |
AU2001286843A1 (en) | 2002-03-13 |
CN1471577A (zh) | 2004-01-28 |
EA200300317A1 (ru) | 2003-10-30 |
US20060057723A1 (en) | 2006-03-16 |
US20060195936A1 (en) | 2006-08-31 |
IL154678A0 (en) | 2003-09-17 |
CA2420724A1 (en) | 2002-03-07 |
US20040103454A1 (en) | 2004-05-27 |
HN2001000250A (es) | 2002-03-13 |
US7192771B2 (en) | 2007-03-20 |
EP1313868B1 (en) | 2006-07-19 |
ES2267809T3 (es) | 2007-03-16 |
JP2004507250A (ja) | 2004-03-11 |
GT200100176A (es) | 2002-04-12 |
DE60121603D1 (de) | 2006-08-31 |
US6911541B2 (en) | 2005-06-28 |
US20060236434A1 (en) | 2006-10-19 |
US20070016975A1 (en) | 2007-01-18 |
US20060191039A1 (en) | 2006-08-24 |
CN1330753C (zh) | 2007-08-08 |
EP1724355A2 (en) | 2006-11-22 |
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