HN2001000250A - Plantas transgenicas conteniendo ansuelos moleculares que alteran el contenido proteinico - Google Patents

Plantas transgenicas conteniendo ansuelos moleculares que alteran el contenido proteinico

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Publication number
HN2001000250A
HN2001000250A HN2001000250A HN2001000250A HN2001000250A HN 2001000250 A HN2001000250 A HN 2001000250A HN 2001000250 A HN2001000250 A HN 2001000250A HN 2001000250 A HN2001000250 A HN 2001000250A HN 2001000250 A HN2001000250 A HN 2001000250A
Authority
HN
Honduras
Prior art keywords
transgenic plants
gene
protein content
protein
seq
Prior art date
Application number
HN2001000250A
Other languages
English (en)
Inventor
Mark A Confling
Yan Li
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Publication of HN2001000250A publication Critical patent/HN2001000250A/es

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/09Recombinant DNA-technology
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1077Pentosyltransferases (2.4.2)

Abstract

LA PRESENTE INVENCION DESCRIBE UN PROCESO PARA LA PRODUCCION DE PLANTAS TRANSGENICAS TALES COMO EL TABACO TRANSGENICO CON CONTENIDO PROTEINICO ALTERADO, QUE LLEVA A FENOTIPOS ALTERADOS TALES COMO NIVELES DE NICOTINA REDUCIDOS, CON LAS PLANTAS TRANSGENICAS ASI PRODUCIDAS Y SEMILLAS PARA TALES PLANTAS. EL PRIMER ASPECTO DE LA PRESENTE INVENCION ES UNA MOLECULA DE ACIDO NUCLEICO (e.g.a plasmid)) CONSISTENTE EN UN ELEMENTO REGULATORIO DE CIS-ACTIVO, Y EL USO DE TAL UN ACIDO NUCLEICO AISLADO PARA LA PRODUCCIÓN DE PLANTA TRANGENICA O CELULA HUESPED HABIENDO ALTERADO LOS NIVELES (a.g. NIVELES AUMENTADOS O DECRECIENTES) DE UNA PROTEINTA DE INTERESES ALLI CONTENIDA. UN EJEMPLO PARTICULAR ES UN PRODUCTO DE GENE NIC DE ELEMENTO RESPENSIVO TAL COMO (a) ACIDONUCLEICO AISLADO TENIENDO UNA SECUENCIA DE ACUERDO AL SEQ ID NO: 1 Y (B) ACIDOS NUCLEICOS AISLADO QUE HIBRIDIZAN AL COMPLEMENTO DE SEQ ID NO:1 Y DOBLAN O SON DE ALGUNA OTRA MANERA RESPONSIVO AL PRODUCTO DE GENE Ni (i. e. TRANSCRIPCIÓN AUMENTADAD O DECRECIENTE DE UN GENE ASOCIADO OPERANDO Y POR ENDE AUMENTA O DECRECE EL NIVEL DE PROTEINA CODIFICADA DE INTEREST EN LAS CELULAS HUESPED.
HN2001000250A 2000-08-30 2001-10-31 Plantas transgenicas conteniendo ansuelos moleculares que alteran el contenido proteinico HN2001000250A (es)

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US22919800P 2000-08-30 2000-08-30

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US (8) US7192771B2 (es)
EP (2) EP1724355A3 (es)
JP (1) JP2004507250A (es)
KR (1) KR20030029885A (es)
CN (1) CN1330753C (es)
AR (1) AR030513A1 (es)
AT (1) ATE333506T1 (es)
AU (1) AU2001286843A1 (es)
CA (1) CA2420724A1 (es)
DE (1) DE60121603T2 (es)
EA (1) EA200300317A1 (es)
ES (1) ES2267809T3 (es)
GT (1) GT200100176A (es)
HN (1) HN2001000250A (es)
IL (1) IL154678A0 (es)
PE (1) PE20020259A1 (es)
UY (1) UY26917A1 (es)
WO (1) WO2002018607A2 (es)

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CN1330753C (zh) 2007-08-08
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DE60121603T2 (de) 2007-06-21
EA200300317A1 (ru) 2003-10-30
US20070016975A1 (en) 2007-01-18
WO2002018607A3 (en) 2003-02-27
EP1313868A2 (en) 2003-05-28
US6911541B2 (en) 2005-06-28
US20040103454A1 (en) 2004-05-27
UY26917A1 (es) 2001-10-25
ATE333506T1 (de) 2006-08-15
US20060242730A1 (en) 2006-10-26
WO2002018607A2 (en) 2002-03-07
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GT200100176A (es) 2002-04-12
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CN1471577A (zh) 2004-01-28
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IL154678A0 (en) 2003-09-17
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