AR014653A1 - Genes y promotores de la familia del maiz pr1, molecula de acido nucleico aislada, construccion de adn, vector, celula huesped, metodo para inducir laexpresion de una secuencia nucleotidica heterologa en una planta, metodo para expresar en forma constituyente una secuencia nucleotidica heterologa en - Google Patents

Genes y promotores de la familia del maiz pr1, molecula de acido nucleico aislada, construccion de adn, vector, celula huesped, metodo para inducir laexpresion de una secuencia nucleotidica heterologa en una planta, metodo para expresar en forma constituyente una secuencia nucleotidica heterologa en

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AR014653A1
AR014653A1 ARP990100801A ARP990100801A AR014653A1 AR 014653 A1 AR014653 A1 AR 014653A1 AR P990100801 A ARP990100801 A AR P990100801A AR P990100801 A ARP990100801 A AR P990100801A AR 014653 A1 AR014653 A1 AR 014653A1
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Argentina
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sequence
nucleotide sequence
nucleotide
expression
sec
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ARP990100801A
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Pioneer Hi Bred Int
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Secuencias nucleotídicas para promotores inducibles y constitutivos aislados a partir de una familia de genes de maíz que codifican las proteínasrelacionadas con patogénesis (PR-1), secuencias nucleotídicas para estos genes PR-1 de maíz, y secuencias de aminoácidos para las proteínas PR-1 codificada porlos mismos. Las secuencias nucleotídicas son utiles para regular la expresion de genes y para mejorar la resistencia a patogenos en plantas. Métodos pararegular la expresion de una secuencia nucleotídica heterologa en una planta de manera inducible o constitutiva, más particularmente, para regular la expresionde un gen PR-1 con el fin de mejorar la resistencia a enfermedades en una planta. Plantas transformadas, semillas y células de plantatransformadas conresistencia a enfermedades mejorada.Molécula de ácido nucleico aislada que tiene una secuencia nucleotídica seleccionada del grupo que consiste en:a) unasecuencia nucleotídica que comprende la secuencia mostrada en la SEC.ID. N* 1,2, 3 o 4; b) una secuencia nucleotídica que comprende por lo menos 40nucleotidos contiguos de la secuencia mostrada en la SEC. ID. N* 1, 2, 3 o 4; c) una secuencia nucleotídica que se híbrida bajo condiciones rigurosas con unasecuencia de a) o b); d)una secuencia nucleotídica que comprende la secuencia mostrada en SEC.ID. N* 5; e) una secuencia nucleotídica que comprende al menos40 nucleotidos contiguos de la secuencia mostrada en SEC. ID. N* 5; f) una secuencia nucleotídica que hibridiza bajocondiciones rigurosas a una secuenciade d) o e); g) una secuencia nucleotídica que comprende la secuencia mostrada en SEC. ID. N* 6, 8, 10 o 14; h) una secuencia nucleotídica que codificaun polipéptido que comprende la secuencia de aminoácidos mostrada en SEC. ID. N* 7, 9, 11 o 15; i) una secuencia nucleotídica que comprende al menos 16nucleotidos contiguos de una sencuencia de g) o h), e i) una secuencia nucleotídica que hibridiza bajo condiciones rigurosas a una secuencia de g), h) o i).
ARP990100801A 1998-02-26 1999-02-25 Genes y promotores de la familia del maiz pr1, molecula de acido nucleico aislada, construccion de adn, vector, celula huesped, metodo para inducir laexpresion de una secuencia nucleotidica heterologa en una planta, metodo para expresar en forma constituyente una secuencia nucleotidica heterologa en AR014653A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7610098P 1998-02-26 1998-02-26
US7964898P 1998-03-27 1998-03-27

Publications (1)

Publication Number Publication Date
AR014653A1 true AR014653A1 (es) 2001-03-28

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ARP990100801A AR014653A1 (es) 1998-02-26 1999-02-25 Genes y promotores de la familia del maiz pr1, molecula de acido nucleico aislada, construccion de adn, vector, celula huesped, metodo para inducir laexpresion de una secuencia nucleotidica heterologa en una planta, metodo para expresar en forma constituyente una secuencia nucleotidica heterologa en

Country Status (6)

Country Link
US (2) US6429362B1 (es)
EP (1) EP1056862A1 (es)
AR (1) AR014653A1 (es)
AU (1) AU754376B2 (es)
CA (1) CA2315549A1 (es)
WO (1) WO1999043819A1 (es)

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